Article
Genetics & Heredity
Ryosuke Tokuda, Nozomu Iwabuchi, Yugo Kitazawa, Takamichi Nijo, Masato Suzuki, Kensaku Maejima, Kenro Oshima, Shigetou Namba, Yasuyuki Yamaji
Summary: Phytoplasmas are bacteria that infect plants and cause abnormal floral organ development. They possess phyllogens, which are effector proteins responsible for this abnormal development. This study analyzed the synteny of phyllogen flanking genomic regions in 17 phytoplasma strains and found that phyllogens are often flanked by potential transposable elements. These elements drive the horizontal transfer of phyllogens among different phytoplasma species and strains. These findings shed light on the sharing of symptom-determinant genes among phytoplasmas.
FRONTIERS IN GENETICS
(2023)
Article
Multidisciplinary Sciences
Gabriela N. Ramos-Lizardo, Jonathan J. Mucherino-Munoz, Eric R. G. R. Aguiar, Carlos Priminho Pirovani, Ronan Xavier Correa
Summary: This study aimed to predict genes encoding effector proteins of C. cacaofunesta through a comparative analysis of Ceratocystis species genomes. A total of 160 candidate effector proteins were identified, with 102 categorized as cytoplasmic and 58 as apoplastic. The study also generated a database containing information on pathogenicity-related genes involved in plant interactions.
SCIENTIFIC REPORTS
(2023)
Review
Plant Sciences
Amelia H. Lovelace, Sara Dorhmi, Michelle T. Hulin, Yufei Li, John W. Mansfield, Wenbo Ma
Summary: Effectors play a crucial role in plant-pathogen interactions, serving as key virulence proteins that are essential for disease development. Understanding the mechanisms of effector virulence has provided valuable insights into microbial pathogenesis and disease resistance. Effector recognition by immune receptors and subsequent immune signaling pathways are important for implementing resistance.
Review
Microbiology
Xinyu Lu, Jinlu Miao, Danyu Shen, Daolong Dou
Summary: Anthracnose caused by plant pathogenic Colletotrichum fungi results in significant economic losses in global field crop production. Understanding the effector proteins of Colletotrichum pathogens is crucial for resistance breeding and disease management strategies. Genomic and bioinformatics tools have identified a large repertoire of putative effectors in Colletotrichum genomes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Zhiyuan Yin, Nan Wang, Weiwei Duan, Lei Pi, Danyu Shen, Daolong Dou
Summary: The CBM1 family is predominantly found in fungi and oomycetes, playing crucial roles in plant-microbe interactions. PcCBP3, identified in Phytophthora capsici, is an apoplastic effector that induces cell death in plants, suggesting its importance in the virulence of the pathogen.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Food Science & Technology
Marc J. A. Stevens, Nicole Cernela, Roger Stephan, Angelika Lehner
Summary: Sequencing of Cronobacter sakazakii strains from a powdered infant formula plant over a 15-year period revealed two major sequence types, ST83 and ST4. Genomic comparisons showed clonality of most ST83 and some ST4 strains, with population-specific genes potentially involved in persistence, such as aggregation and stress-resistance genes in ST83 strains and fimbrial genes in ST4 strains. Several antimicrobial resistance genes, including a colistin resistance gene acquired via horizontal gene transfer, were found in these strains. The findings suggest that evolving C. sakazakii populations in an industrial environment may have an impact on public health.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
You Che, Yu Yang, Xiaoqing Xu, Karel Brinda, Martin F. Polz, William P. Hanage, Tong Zhang
Summary: The study found that plasmid-borne antimicrobial resistance genes are mainly enriched in conjugative plasmids. Scientists reported the discovery of a massive insertion sequence-associated antimicrobial resistance gene transfer network, suggesting a general evolutionary mechanism for horizontal gene transfer mediated by the interaction between conjugative plasmids and ISs. Experimental results confirmed the importance of these interactions in aiding the horizontal transfer and expanding the genetic range of antimicrobial resistance genes within complex microbial communities.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Georgios Tzelepis, Fredrik Doelfors, Louise Holmquist, Christina Dixelius
Summary: A novel fungal effector RsCRP1 was discovered targeting distinct plant cell organelles to facilitate successful infection in the early stages of disease development. RsCRP1 localized to chloroplasts and mitochondria and failed to induce necrosis or suppress hypersensitive response in Nicotiana benthamiana.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Lirong Han, Yubo Sun, Xin Zhou, Xinchang Hao, Meng Wu, Xing Zhang, Juntao Feng
Summary: The novel glycoprotein GP-1 from Streptomyces sp. ZX01 induces plant immunity and enhances viral resistance in Nicotiana benthamiana by rapidly inducing Ca2+ influx, reactive oxygen species (ROS) and nitric oxide (NO). Transcriptome analysis revealed upregulated defense-related pathways and gene expression in GP-1-treated plants. Furthermore, GP-1 promotes salicylic acid (SA) and jasmonic acid (JA) contents, strengthening plant secondary metabolites activities and systemic accumulation of pathogenesis-related proteins for antiviral activity against TMV.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Microbiology
Neha Sahu, Boris Indic, Johanna Wong-Bajracharya, Zsolt Merenyi, Huei-Mien Ke, Steven Ahrendt, Tori-Lee Monk, Sandor Kocsube, Elodie Drula, Anna Lipzen, Balazs Balint, Bernard Henrissat, Bill Andreopoulos, Francis M. Martin, Christoffer Bugge Harder, Daniel Rigling, Kathryn L. Ford, Gary D. Foster, Jasmyn Pangilinan, Alexie Papanicolaou, Kerrie Barry, Kurt LaButti, Mate Viragh, Maxim Koriabine, Mi Yan, Robert Riley, Simang Champramary, Krista L. Plett, Igor V. Grigoriev, Isheng Jason Tsai, Jason Slot, Gyoergy Sipos, Jonathan Plett, Laszlo G. Nagy
Summary: This study reveals that the fungal genus Armillaria has undergone horizontal gene transfer, which may have affected its ability to degrade plant biomass and cause diseases, and explains its unusual soft rot-like wood decay strategy.
NATURE MICROBIOLOGY
(2023)
Review
Plant Sciences
Qi-Qing Cheng, Yue Ouyang, Zi-Yu Tang, Chi-Chou Lao, Yan-Yu Zhang, Chun-Song Cheng, Hua Zhou
Summary: This article discusses the importance and widespread application of medicinal plant genome research with the development of sequencing technology, providing important guidance and support for breeding, evolutionary history, herbal synthesis, etc. in medicinal plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Abdullah Ibn Mafiz, Yingshu He, Wei Zhang, Yifan Zhang
Summary: The study revealed potential antimicrobial resistance in soil and vegetable samples from Detroit community gardens, with a high percentage of bacteria showing resistance to various antibiotics. Gene transfer was observed among bacterial isolates, indicating a possibility of resistance spread.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Minkyu Park, Ali Sarkhosh, Violeta Tsolova, Islam El-Sharkawy
Summary: Horizontally transferred transposable elements play a significant role in plant genome evolution by generating additional TE subfamilies and causing differential proliferation in host genomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biology
Emilie Aubin, Moaine El Baidouri, Olivier Panaud
Summary: Plants maintain species integrity through vertical transmission of genetic information during reproduction, but recent reports have shown that horizontal gene transfers are common in nature, involving all organisms including plants. The advent of next-generation sequencing technologies has provided new perspectives for studying HGTs.
Article
Immunology
James B. Pettengill, Jennifer Beal, Maria Balkey, Marc Allard, Hugh Rand, Ruth Timme
Summary: Open-source DNA sequence databases are beneficial for public health, but incomplete and non-standard metadata can impact interpretative labor costs and foodborne outbreak detection and response. Improving the quality of sequence-associated metadata can lead to earlier detection of emerging food safety hazards and faster response to outbreaks.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Plant Sciences
Alexandra J. Weisberg, Yu wu, Jeff H. Chang, Erh-Min Lai, Chih-Horng Kuo
Summary: Agrobacteria are feared as agricultural pathogens, causing abnormalities and economic damage to plants, but they are also revered in the laboratory for their usefulness as models and tools in biological research. This review synthesizes elegant work on agrobacterial virulence mechanisms and ecology, emphasizing their genomic and plasmid characteristics, and highlights areas for further research.
ANNUAL REVIEW OF PHYTOPATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yi-Hsin Liu, Tong-Seung Tseng, Ching-Rong Wu, Shu-Ting Cho, Chih-Horng Kuo, Xin-Jie Huang, Jung-Yi Cheng, Kuo-Hsuan Hsu, Kung-Fu Lin, Chia-Chin Liu, Ching-Hui Yeh
Summary: The small heat shock proteins (sHSPs) OsHsp16.9A and OsHsp18.0 have been found to accumulate in response to heat shock and play different roles in thermotolerance in rice. Mass spectrometry analysis revealed that OsHsp16.9A forms a stable complex with OsHsp101, and specific amino acid residues of OsHsp16.9A are essential for this interaction. The interaction between OsHsp16.9A and OsHsp101 is crucial for enhancing thermotolerance in rice plants.
Article
Microbiology
Si-Chong Wang, Ai-Ping Chen, Shu-Jen Chou, Chih-Horng Kuo, Erh-Min Lai
Summary: The T6SS is used by proteobacteria to secrete effector proteins and influence bacterial competition or pathogenesis. In this study, the researchers demonstrated that the T6SS affects disease occurrence and the composition of the microbial community in crown galls.
Article
Infectious Diseases
Ivana Goic-Barisic, Martina Seruga Music, Marina Drcelic, Semra Tuncbilek, Gulcin Akca, Sanja Jakovac, Marija Tonkic, Jasna Hrenovic
Summary: The aim of this study was to characterize 11 colistin- and carbapenem-resistant Acinetobacter baumannii isolates recently emerging in hospital settings. A. baumannii isolates were collected from hospitalised patients under colistin treatment in Turkey, Croatia, and Bosnia and Herzegovina. The isolates were highly resistant to colistin and had point mutations in pmrCAB operon genes. The pattern of point mutations suggests a spread of specific colistin-resistant isolates within the hospital.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Agronomy
Shih-Hsun Walter Hung, Tsai-Ching Huang, Yu-Chih Lai, I-Chen Wu, Chia-Ho Liu, Yuh-Feng Huarng, Hau-Hsuan Hwang, En-Pei Isabel Chiang, Chih-Horng Kuo, Chieh-Chen Huang
Summary: Using Burkholderia seminalis 869T2 as a biostimulant can enhance the growth and quality of leafy vegetables, reduce fertilizer usage, and decrease costs while increasing product value.
Article
Microbiology
Emina Pustijanac, Jasna Hrenovic, Mirna Vranic-Ladavac, Martina Mocenic, Natalie Karcic, Lorena Lazaric Stefanovic, Irena Hrstic, Jasenka Loncaric, Martina Seruga Music, Marina Drcelic, Dijana Majstorovic, Ines Kovacic
Summary: The aim of this study was to identify the source of Acinetobacter baumannii in the ICU after a COVID-19 outbreak, as it was not found on usually screened surfaces. A single A. baumannii isolate was recovered from the air conditioner and compared to clinical isolates from patients. Molecular and antibiotic resistance analysis confirmed that the isolate from the air conditioner was the same strain as those from patients. The study highlights the importance of disinfecting hospital air conditioners to prevent A. baumannii outbreaks.
Article
Microbiology
Yalu Li, Xiao-Hua Yan, Yanzhi Liu, Shen-Chian Pei, Chih-Horng Kuo, Weijie Huang
Summary: This work presents the complete genome sequence of Candidatus Phytoplasma aurantifolia TB2022, a bacterium associated with sweet potato little leaf disease in Fujian Province, China. The genome consists of one 670,073-bp circular chromosome.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Xiao-Hua Yan, Jixiu Lin, Yanzhi Liu, Peizhi Huang, Jianmi Liu, Qibin Hu, Yalu Li, Shen-Chian Pei, Weijie Huang, Chih-Horng Kuo
Summary: The complete genome sequence of Candidatus Phytoplasma asteris QS2022, a bacterium associated with lettuce chlorotic leaf rot disease in Fujian Province, China, is presented in this study. The genome consists of a single 834,303-bp circular chromosome.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Biology
Thomas C. C. Mathers, Roland H. M. Wouters, Sam T. T. Mugford, Roberto Biello, Cock van Oosterhout, Saskia A. A. Hogenhout
Summary: The English and Indian grain aphids belong to a cryptic species complex with highly differentiated lineages. Hybridization has significantly contributed to grain aphid diversity, increasing the evolutionary potential of this important pest species.
Article
Microbiology
Shen-Chian Pei, Ai-Ping Chen, Shu-Jen Chou, Ting-Hsuan Hung, Chih-Horng Kuo
Summary: This study presents the complete genome sequence of Candidatus Phytoplasma pruni strain PR2021, which includes one 705,138 bp circular chromosome and one 4,757 bp circular plasmid, and is associated with the poinsettia cultivar Princettia Pink.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
John Raymond B. Salazar, Mary Nia M. Santos, Jeremy L. Palaad, Hsi-Ching Yen, Jose Louis A. Castellano, Leobert D. dela Pena, Edgar C. Amar, Erh-Min Lai, Chih-Horng Kuo
Summary: This study reports the complete genome sequence of Vibrio parahaemolyticus strain PH1273 collected from a Penaeus vannamei pond in the Philippines in 2015. Genome analysis revealed the absence of the pirAB gene responsible for causing acute hepatopancreatic necrosis disease, but the presence of multiple secretion systems and their associated effectors.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Shu-Ting Cho, Ai-Ping Chen, Shu-Jen Chou, Xiao-Hua Yan, Chan-Pin Lin, Chih-Horng Kuo
Summary: This study presents the complete genome sequence of Candidatus Phytoplasma cynodontis strain GY2015, which is an uncultivated plant-pathogenic bacterium associated with Bermuda grass white leaf disease in Taoyuan, Taiwan.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Correction
Plant Sciences
Jelena Plavec, Martina Seruga Music
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Jelena Plavec, Martina Seruga Music
Summary: Flavescence doree phytoplasma (FDp) poses a major threat to grapevine cultivation in Europe. A multiplex real-time PCR assay has been developed to detect the three genetic clusters of FDp, providing a rapid and sensitive method for early detection and discrimination.
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Microbiology
Shih-Hsun Walter Hung, I-Chen Wu, Chieh-Chen Huang, Chih-Horng Kuo
Summary: This study reports the isolation and genome sequence of a coral-associated Erythrobacteraceae bacterium, strain WH01K, which consists of a circular chromosome of 2,745,896 bp and a circular plasmid of 172,502 bp.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)