Journal
SENSORS
Volume 19, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/s19010052
Keywords
influenza A; hemagglutinin; H1N1; Quenchbody; immunosensor
Funding
- JSPS KAKENHI from the Japan Society for the Promotion of Science [JP15H04191, JP18H03851, JP26420793]
- Nakatani Foundation, Japan
- National Research Foundation of Korea - Korean government (MSIT) [2018R1C1B5044988]
- 2018 Hongik University Research Fund, Korea
- Natural Science Foundation of the Shandong Province [ZR2017MB037]
- National Natural Science Foundation of China [21775064]
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We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.
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