Article
Multidisciplinary Sciences
Laura Bernal, Pamela Sotelo-Hitschfeld, Christine Koenig, Viktor Sinica, Amanda Wyatt, Zoltan Winter, Alexander Hein, Filip Touska, Susanne Reinhardt, Aaron Tragl, Ricardo Kusuda, Philipp Wartenberg, Allen Sclaroff, John D. Pfeifer, Fabien Ectors, Andreas Dahl, Marc Freichel, Viktorie Vlachova, Sebastian Brauchi, Carolina Roza, Ulrich Boehm, David E. Clapham, Jochen K. Lennerz, Katharina Zimmermann
Summary: This study clarifies the molecular and cellular components of the dental cold sensing system, highlighting the role of TRPC5 and TRPA1 in cold sensing in teeth. Odontoblasts are shown to be the direct site of cold transduction. This expands our understanding of the mechanisms behind cold sensitivity in teeth.
Article
Pharmacology & Pharmacy
M. Veronica Donoso, Felipe Hernandez, Rafael Barra, J. Pablo Huidobro-Toro
Summary: This study evaluated the effects of the bisphosphonate clodronate on ATP/metabolites and NA overflow in sympathetic perivascular nerve terminals. The results showed that clodronate did not affect the time course of ATP or NA overflow induced by nerve terminal depolarization, but it significantly increased perfusate adenosine concentration at a concentration of 10 nM.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Keita Tatsushima, Nao Hasuzawa, Lixiang Wang, Miki Hiasa, Shohei Sakamoto, Kenji Ashida, Nobuyuki Sudo, Yoshinori Moriyama, Masatoshi Nomura
Summary: The study found that VNUT plays a crucial role in the progression of NASH by regulating vesicular ATP release to control triglyceride secretion in hepatocytes and involve in chronic inflammation. Blockade of vesicular ATP release through VNUT inhibition protects against the progression of steatohepatitis, suggesting VNUT may be a potential pharmacological target for NASH.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Physiology
Mayumi Matsunaga, Maki Kimura, Takehito Ouchi, Takashi Nakamura, Sadao Ohyama, Masayuki Ando, Sachie Nomura, Toshifumi Azuma, Tatsuya Ichinohe, Yoshiyuki Shibukawa
Summary: Odontoblasts play crucial roles in dentin formation and sensory transduction. Mechanical stimulation induces intracellular Ca2+ signaling through Piezo1 channels, promoting intercellular communication and regulating dentinogenesis. Understanding the role of Piezo1 channels in odontoblast function may provide insights into dentin sensitivity and mineralization processes.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Medicine, Research & Experimental
Cai-rong Dong, Wen-jun Zhang, Hong-liang Luo
Summary: Neuropathic pain is a common symptom that greatly affects the physical and mental health of patients. Researchers are increasingly interested in exploring potential therapeutic strategies for neuropathic pain, with a focus on the role of P2X3 receptors in pain development. This paper summarizes the structure and characteristics of the P2X3 receptor gene, discusses its function in the nervous system, and provides valuable information for the development of novel pain treatment strategies.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Nao Hasuzawa, Sawako Moriyama, Lixiang Wang, Ayako Nagayama, Kenji Ashida, Yoshinori Moriyama, Masatoshi Nomura
Summary: Quinacrine, a fluorescent amphipathic amine, is not a suitable fluorescent probe for vesicular ATP storage as its accumulation in vesicles is not due to interaction with ATP but driven by pH gradient as an amphipathic amine.
PURINERGIC SIGNALLING
(2021)
Review
Cell Biology
Georg A. A. Petroianu, Lujain Aloum, Abdu Adem
Summary: Physiopathology and neurotransmission of pain are complex, and our ability to suppress chronic pain is limited. The number of clinically available medications is low and their success is modest. Pain Medicine practitioners face an ethical dichotomy, as they strive to adhere to the principle of primum non nocere while also aiming for successful pain relief. This article provides an overview of nociceptive pain, including nociceptive input, modulatory output, and local control. It also discusses the transition to neuropathic pain and the process of pain sensitization and chronification. The article then explores the sites and modes of action of clinically available drugs used in chronic pain treatment, with a focus on adjuvant medication.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Immunology
Teruyuki Ishikura, Makoto Kinoshita, Mikito Shimizu, Yoshiaki Yasumizu, Daisuke Motooka, Daisuke Okuzaki, Kazuya Yamashita, Hisashi Murata, Shohei Beppu, Toru Koda, Satoru Tada, Naoyuki Shiraishi, Yasuko Sugiyama, Katsuichi Miyamoto, Susumu Kusunoki, Tomoyuki Sugimoto, Atsushi Kumanogoh, Tatsusada Okuno, Hideki Mochizuki
Summary: The study demonstrates the release of extracellular ATP from astrocytes induced by anti-AQP4 antibody. The ATP-mediated development of mechanical allodynia was also suggested in rats treated with anti-AQP4 antibody. The pivotal role of ATP in the pain mechanism of NMOSD is indicated by the study.
JOURNAL OF NEUROINFLAMMATION
(2021)
Review
Medicine, Research & Experimental
Cai-Rong Dong, Wen-Jun Zhang, Hong-Liang Luo
Summary: Neuropathic pain is a common symptom that greatly affects the physical and mental health of patients. The role of P2X3 receptors in the development of neuropathic pain has been extensively studied, as they participate in neural transmission and related signaling pathways. This paper provides a summary of the structure and characteristics of P2X3 receptors, discusses their role in the nervous system, outlines the pathological mechanisms of pain, and describes the relationship between P2X3 receptors and chronic pain, offering valuable information for the development of novel treatment strategies for pain.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Pharmacology & Pharmacy
Nao Hasuzawa, Keita Tatsushima, Rie Tokubuchi, Masaharu Kabashima, Masatoshi Nomura
Summary: ATP, used as an energy source in cells, also functions as an extracellular signaling molecule. Studies on purinergic receptor subtypes have shown its significant roles in different cell types. VNUT, a vital transporter in the SLC17 family, plays a crucial role in storing and releasing ATP in cells, impacting chronic inflammation and metabolic diseases like type 2 diabetes and NAFLD.
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
(2021)
Article
Immunology
Charlie H. T. Kwok, Yuta Kohro, Michael Mousseau, Melissa S. O'Brien, John R. Matyas, Jason J. McDougall, Tuan Trang
Summary: Painful injury increases afferent input, enhancing the activity of central nociceptive circuits through neuron-neuron and neuron-glia interactions. Microglia play a crucial role in the pathogenesis of chronic pain, and peripheral joint injury signals the CNS to engage spinal microglial responses.Joint nociceptors are activated by early peripheral joint injury, triggering a central spinal microglial response and potentially modulating communication between sensory neurons and spinal microglia through ATP signaling during joint degeneration.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Yongqiang Shi, Chaoyang Gong, Wei Nan, Wenming Zhou, Zeyuan Lei, Kaisheng Zhou, Linna Wang, Guanghai Zhao, Haihong Zhang
Summary: Botulinum toxin type A (BoNT/A) directly alleviates neuropathic pain by inhibiting the release of substance P, calcitonin gene-related peptide (CGRP), and glutamate. This study reveals that BoNT/A also affects the expression of vesicular nucleotide transporter (VNUT), a key regulator of ATP release in neuropathic pain. By decreasing VNUT expression, BoNT/A exhibits significant analgesic activity in chronic constriction injury of the sciatic nerve (CCI) induced neuropathic pain in rats. Moreover, overexpression of VNUT reverses the antinociceptive effect of BoNT/A.
Article
Neurosciences
Shi-qi Hu, Jia-ling Hu, Fei-long Zou, Ji-peng Liu, Hong-liang Luo, Dong-xia Hu, Li-dong Wu, Wen-jun Zhang
Summary: Different studies have confirmed the crucial role of P2X7 receptor-mediated inflammatory mediators in the development of pain. By mediating the release of inflammatory mediators, activation of P2X7 receptors can induce pain. This paper discusses the pathological mechanism of P2X7 receptor-mediated inflammation and pain, focusing on the internal relationship between P2X7 receptor and pain. The effects of some antagonists on pain relief by inhibiting the activities of P2X7 receptors are also described.
BRAIN RESEARCH BULLETIN
(2022)
Article
Biology
Stephen P. Finch, Michael D. D'Emic
Summary: A study found that there is no relationship between body mass and daily dentine apposition rate (DDAR) in non-ever-growing teeth of amniotes. Other factors such as metabolic rate, number of tooth generations, diet, and habitat also do not predict or correspond with DDARs. Large terrestrial mammals, dinosaurs, and marine reptiles have similar DDARs, while primates, cetaceans, and some smaller marine reptiles have independently evolved exceptionally slow rates. Life-history factors may explain the evolution of dentine apposition rates.
Article
Endocrinology & Metabolism
Kazumi Kawata, Keishi Narita, Ayako Washio, Chiaki Kitamura, Tatsuji Nishihara, Satoshi Kubota, Sen Takeda
Summary: This study investigates the function of primary cilia in dentin-producing odontoblasts and highlights their role in regulating odontoblastic differentiation through the canonical Wnt signaling pathway. Additionally, the study suggests the existence of a feedback loop between primary cilia and the canonical Wnt pathway.