Article
Immunology
Brian T. Fisher, Craig L. K. Boge, Rui Xiao, Sydney Shuster, Dawn Chin-Quee, John Allen, Shareef Shaheen, Randall Hayden, Sri Suganda, Theoklis E. Zaoutis, Yeh Chung Chang, Dwight E. Yin, Anna R. Huppler, Lara Danziger-Isakov, William J. Muller, Emmanuel Roilides, Jose Romero, Paul K. Sue, David Berman, Rachel L. Wattier, Natasha Halasa, Alice Pong, Gabriela Maron, Pere Soler-Palacin, Susan C. Hutto, Blanca E. Gonzalez, Christine M. Salvatore, Sujatha Rajan, Michael Green, Elizabeth Doby Knackstedt, Sarmistha B. Hauger, William J. Steinbach
Summary: This study suggests that the T2Candida assay alone or in combination with the Platelia CandidaAg Plus assay can be beneficial for rapid detection of invasive candidiasis in children.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Helena de Puig, Rose A. Lee, Devora Najjar, Xiao Tan, Luis R. Soekensen, Nicolaas M. Angenent-Mari, Nina M. Donghia, Nicole E. Weckman, Audrey Ory, Carlos F. Ng, Peter Q. Nguyen, Angelo S. Mao, Thomas C. Ferrante, Geoffrey Lansberry, Hani Sallum, James Niemi, James J. Collins
Summary: The miSHERLOCK is a low-cost CRISPR-based POC diagnostic platform that can rapidly detect SARS-CoV-2 and its variants with high sensitivity in just 1 hour. This modular system can be quickly adapted to different viruses and variants, and is complemented by a smartphone application for output interpretation and result reporting.
Article
Chemistry, Analytical
Meiting Yang, Yidan Tang, Lijuan Qi, Sicai Zhang, Yichen Liu, Baiyang Lu, Jiaxue Yu, Kun Zhu, Bingling Li, Yan Du
Summary: A portable and accurate nucleic acid detection system was developed to address the urgent need for on-site virus screening and infection control. It utilizes ultrasensitive isothermal amplification to achieve rapid and sensitive detection of SARS-CoV-2 RNA, providing a cost-effective solution for emergency detection of the virus.
ANALYTICAL CHEMISTRY
(2021)
Review
Microbiology
Kyaw M. Hlaing, Lea M. Monday, Marcio Nucci, Simone A. Nouer, Sanjay G. Revankar
Summary: The COVID-19 pandemic has caused over 6 million deaths worldwide, largely due to respiratory failure. Hospitalized patients, especially those in the intensive care unit, frequently experience complications. Fungal infections, including invasive aspergillosis, candidiasis, and mucormycosis, have posed significant morbidity and mortality risks. Risk factors include changes in immune defenses caused by COVID-19 and immunosuppression from various therapies in severely ill patients. Diagnosis is often challenging due to limited sensitivity of current tests. Overall outcomes are poor, with mortality rates exceeding 50% in some studies, primarily due to comorbidities and delayed diagnosis. Early diagnosis and appropriate antifungal therapy require a high index of clinical suspicion.
Article
Chemistry, Analytical
Xiuen Cao, Chuanpin Chen, Qubo Zhu
Summary: This review summarizes the applications of functional nucleic acids and isothermal amplification techniques in biosensing, highlighting their important contributions to the field. These technologies have great potential to enhance the performance of biosensors.
Review
Multidisciplinary Sciences
Maria C. Cardona-Trujillo, Tatiana Ocampo-Cardenas, Fredy A. Tabares-Villa, Augusto Zuluaga-Velez, Juan C. Sepulveda-Arias
Summary: This systematic review explores the advances in the molecular diagnosis of Zika and Chikungunya viruses in humans, emphasizing the need for standardization in reporting and interpreting new diagnostic methods. It also highlights the benefits of new alternatives for the molecular diagnosis of these arboviruses compared to established methods.
Review
Food Science & Technology
Tai-Yong Kim, Xiaoning Zhu, Se-Min Kim, Jeong-A Lim, Min-Cheol Lim, Ke Luo
Summary: Viruses are major pathogens in food poisoning, and the development of foodborne virus detection technologies for the entire food distribution chain is crucial for food safety. Polymerase chain reaction (PCR) is the gold standard method for monitoring food contamination by viruses, but it is time-consuming and vulnerable to inhibitors in food samples. Optical and electrical readout biosensors based on nucleic acid isothermal amplification technology and nanomaterials have emerged as alternatives, offering portability and on-site operation. However, the complexity of food components and lack of stability studies on residual food components limit the practical application of biosensors as a universal detection method.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Yong Chen, Xiaoling Xu, Jiachun Wang, Yibin Zhang, Wentao Zeng, Yizhen Liu, Xueji Zhang
Summary: A photoactivated CRISPR/Cas12a strategy was proposed in this study to achieve high-sensitivity nucleic acid detection in one pot, avoiding amplicon contamination and lowering the threshold for molecular diagnostics in POC.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Nan Zong, Ying Gao, Yong Chen, Xueqing Luo, Xingyu Jiang
Summary: A centrifugal microfluidic platform was developed for combined pretreatment and molecular diagnosis. Multiple signal enhancement systems were used to avoid false-negative results. The platform successfully detected and genotyped hepatitis B virus from whole blood. Compared to traditional diagnostic processes, the platform significantly reduced time consumption and the need for professional labor.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Chemistry, Analytical
Hao Jiang, Yuan Li, Xuefei Lv, Yulin Deng, Xiaoqiong Li
Summary: Nucleic acid amplification techniques, such as polymerase chain reaction (PCR) and isothermal amplification, are important for nucleic acid detection. However, PCR is limited by temperature control and isothermal amplification has its own limitations. Efforts to integrate enzymes and amplification techniques have the potential to overcome these limitations. This review provides a comprehensive summary of cascade amplification, including design fundamentals, signal generation, evolution, applications, challenges, and trends.
Article
Immunology
F. Lamoth, H. Akan, D. Andes, M. Cruciani, O. Marchetti, L. Ostrosky-Zeichner, Z. Racil, C. J. Clancy
Summary: BDG testing may be useful for diagnosing IF, but its performance and utility vary depending on patient population and pretest likelihood of IF.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Nanoscience & Nanotechnology
Fangfei Yin, Haipei Zhao, Shasha Lu, Juwen Shen, Min Li, Xiuhai Mao, Fan Li, Jiye Shi, Jiang Li, Baijun Dong, Wei Xue, Xiaolei Zuo, Xiurong Yang, Chunhai Fan
Summary: The article introduces a DNA-encoded molecular classifier that can classify multidimensional molecular clinical data and has been tested in prostate cancer patients.
NATURE NANOTECHNOLOGY
(2023)
Review
Chemistry, Analytical
Mengtao Chen, Xinyue Lan, Longjiao Zhu, Ping Ru, Haiyan Liu, Wentao Xu
Summary: The safety in agriculture, food, and medicine production and processing is crucial for global economic and social stability. Efficient techniques for microorganism detection, driven by functional nucleic acids (FNAs) and molecular amplification technologies, have greatly improved operation time, sensitivity, and high throughput analysis. This review summarizes novel microorganism detection methods in three aspects: direct to indirect methods, simple to integrated strategies, and representative applications, providing a useful reference and guide for future development of advanced nucleic acid-aided molecular amplification techniques.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Marta Broto, Michael M. Kaminski, Christopher Adrianus, Nayoung Kim, Robert Greensmith, Schan Dissanayake-Perera, Alexander J. Schubert, Xiao Tan, Hyemin Kim, Anand S. Dighe, James J. Collins, Molly M. Stevens
Summary: This study combines CRISPR-Cas reaction with nanozyme-linked ELISA to achieve quantitative detection of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). The study shows that CrisprZyme is adaptable to lateral flow reading and different Cas enzymes, and can detect various non-coding RNAs. The platform can be used to identify disease patients and monitor cellular differentiation. CrisprZyme can serve as a universally applicable signal catalyst for CRISPR-based diagnostics, expanding the target range of preamplification-free, quantitative detection.
NATURE NANOTECHNOLOGY
(2022)
Article
Infectious Diseases
Margarita Estreya Zvezdanova, Manuel J. Arroyo, Gema Mendez, Ana Candela, Luis Mancera, Julio Garcia Rodriguez, Julia Lozano Serra, Rosa Jimenez, Inmaculada Lozano, Carmen Castro, Concepcion Lopez, Patricia Munoz, Jesus Guinea, Pilar Escribano, Belen Rodriguez-Sanchez
Summary: The study demonstrates the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to accurately detect azole resistance in species of the Aspergillus fumigatus complex. The results show that MALDI-TOF MS combined with peak analysis is a novel tool that allows the differentiation of A. fumigatus from other species within the complex and the detection of azole-resistant A. fumigatus isolates.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Mycology
Clara Vinado, Rosa Maria Giron, Elisa Ibanez, Alberto Garcia-Ortega, Ines Perez, Dinora Polanco, Javier Peman, Amparo Sole
Summary: Chronic filamentous fungal colonization in patients with CF is associated with a significant annual decline of lung function, which is linked to poorer quality of life. However, protocolized antifungal therapy can improve lung function, especially in patients with more advanced disease.
REVISTA IBEROAMERICANA DE MICOLOGIA
(2021)
Article
Microbiology
Victor Garcia-Bustos, Amparo Ruiz-Sauri, Alba Ruiz-Gaitan, Ignacio Antonio Sigona-Giangreco, Marta Dafne Cabanero-Navalon, Oihana Sabalza-Baztan, Miguel Salavert-Lleti, Maria Angeles Tormo, Javier Peman
Summary: This study used an in vivo insect model to evaluate the virulence of Candida auris, an emergent multidrug-resistant fungal pathogen. It was found to have an intermediate degree of virulence compared to other known Candida species, with significant variability between strains. The ability of C. auris to filament in vivo and its early dissemination through the host, with a remarkable respiratory tropism, were also documented.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Victor Garcia-Bustos, Javier Peman, Alba Ruiz-Gaitan, Marta Dafne Cabanero-Navalon, Ana Cabanilles-Boronat, Maria Fernandez-Calduch, Lucia Marcilla-Barreda, Ignacio A. Sigona-Giangreco, Miguel Salavert, Maria Angeles Tormo-Mas, Amparo Ruiz-Sauri
Summary: This study analyzes the in vivo behavior of Candida auris compared to other Candida species in G. mellonella. It reveals that C. auris exhibits higher immunogenic activity, unique invasion patterns, and inter-phenotypic tissue tropism differences. The study also highlights the importance of phenotypic identification in clinical settings.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Immunology
Guillermo Quindos, Katherine Miranda-Cadena, Rosario San-Millan, Katyna Borroto-Esoda, Emilia Canton, Maria Jose Linares-Sicilia, Axel Hamprecht, Isabel Montesinos, Anna Maria Tortorano, Anna Prigitano, Matxalen Vidal-Garcia, Cristina Marcos-Arias, Andrea Guridi, Ferran Sanchez-Reus, Jesus Machuca-Barcena, Manuel Antonio Rodriguez-Iglesias, Estrella Martin-Mazuelos, Carmen Castro-Mendez, Leyre Lopez-Soria, Alba Ruiz-Gaitan, Marcelo Fernandez-Rivero, Damaris Lorenzo, Javier Capilla, Antonio Rezusta, Javier Peman, Josep Guarro, Joana Pereira, Celia Pais, Orazio Romeo, Guillermo Ezpeleta, Nerea Jauregizar, David Angulo, Elena Eraso
Summary: This study assessed the in vitro activity of Ibrexafungerp and other drugs against 434 blood isolates of Candida in Europe. The results showed that Ibrexafungerp exhibited potent activity against these isolates and also showed activity against fluconazole- and echinocandin-resistant isolates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Correction
Immunology
E. Moreno-Garcia, P. Puerta-Alcalde, G. Gariup, M. Fernandez-Ruiz, L. E. Lopez Cortes, G. Cuervo, M. Salavert, P. Merino, M. Machado, J. Guinea, J. Garcia-Rodriguez, J. Garnacho-Montero, C. Cardozo, J. Peman, M. Montejo, J. Fortun, B. Almirante, C. Castro, J. Rodriguez-Bano, J. M. Aguado, J. A. Martinez, J. Carratala, A. Soriano, C. Garcia-Vidal
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Microbiology
Juan Vicente Mulet Bayona, Carme Salvador Garcia, Nuria Tormo Palop, Amparo Valentin Martin, Carmelo Gonzalez Padron, Javier Colomina Rodriguez, Javier Peman, Concepcion Gimeno Cardona
Summary: The CHROMagar(TM) Candida Plus (CC-Plus) medium is able to rapidly identify most clinical Candida species and differentiate C. auris. A multicentric study demonstrated that CC-Plus performed well in detecting C. auris and other clinically relevant Candida species in surveillance and environmental cultures.
Article
Dermatology
Julia Serrano-Lobo, Ana Gomez, Elena Reigadas, Patricia Munoz, Pilar Escribano, Jesus Guinea
Summary: This study evaluated the impact of not filtering conidial suspensions on the screening method for A. fumigatus isolates and found that unfiltered suspensions do not negatively affect the performance of the method.
Article
Dermatology
Julia Serrano-Lobo, Ana Gomez, Elena Reigadas, Patricia Munoz, Pilar Escribano, Jesus Guinea
Summary: This study evaluated the performance of gradient diffusion strips (GDSs) in detecting azole resistance in Aspergillus fumigatus. The results showed that GDSs can effectively identify azole-resistant isolates of A. fumigatus sensu stricto, but have poor performance against cryptic species.
Article
Medicine, General & Internal
Alba Ruiz-Gaitan, Ignacio Sigona-Giangreco, Jose Manuel Perez-Royo, Victor Garcia-Bustos, Marta Garcia-Hita, Eulogio Valentin-Gomez, Salvador Giner Almaraz, Piet W. J. de Groot, Javier Peman
Summary: This study describes the morphological characteristics of C. auris colonies on three commercial chromogenic media. CHROMagar (TM) Candida Plus and HiCrome (TM) Candida can provide presumptive identification of C. auris, but identification may be difficult in the presence of mixed cultures. The use of CHROMagar (TM) Candida medium with 32 mg/L fluconazole offers better performance for the identification of C. auris by inhibiting fluconazole-susceptible strains and selecting rare or high fluconazole MIC (>32 mg/L) isolates.
Article
Engineering, Biomedical
Andy Hernandez-Montoto, M. Nieves Aranda, Isabel Caballos, Alba Lopez-Palacios, Maria Angeles Tormo-Mas, Javier Peman, Mireya Prieto Rodriguez, Carlos Picornell, Elena Aznar, Ramon Martinez-Manez
Summary: Fluorogenic probes based on oligonucleotide capped nanoporous anodic alumina films were developed for specific and sensitive detection of human papilloma virus (HPV) DNA. The probes showed high sensitivity and selectivity in detecting 14 different high-risk (hr) HPV types in clinical samples. The optimized synthesis protocol allowed for the production of sensors with high reproducibility and reliable fluorescence signal reading.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Isabel Caballos, Maria Nieves Aranda, Alba Lopez-Palacios, Luis Pla, Sara Santiago-Felipe, Andy Hernandez-Montoto, Maria Angeles Tormo-Mas, Javier Peman, Maria Dolores Gomez-Ruiz, Eva Calabuig, Beatriz Sanchez-Sendra, Clara Frances-Gomez, Ron Geller, Elena Aznar, Ramon Martinez-Manez
Summary: The COVID-19 pandemic has emphasized the importance of testing and tracking infected individuals to mitigate the virus spread. This study introduces a biosensor that uses oligonucleotide-gated nanomaterials for specific detection of the SARS-CoV-2 spike protein. The biosensor showed high sensitivity and successfully detected low concentrations of the pseudotype virus. It was also tested on nasopharyngeal samples from suspected COVID-19 individuals.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Microbiology
Carlos Vallejo, Isidro Jarque, Jesus Fortun, Araceli Casado, Javier Peman
Summary: Recent years have seen an increase in invasive fungal infections (IFIs), particularly caused by non-Candida albicans species, non-Aspergillus molds, and azole-resistant Aspergillus fumigatus. To determine the most common management strategies for IFIs, a cross-sectional multicenter survey involving 55 hematologists and infectious disease specialists in 31 Spanish hospitals was conducted. Key strategies identified include early treatment for persistent febrile neutropenia, switching to another broad-spectrum antifungal if azole-resistant Aspergillus is suspected, prophylactic use of broad-spectrum azoles and echinocandins in patients receiving midostaurin or venetoclax, and the use of liposomal amphotericin B for breakthrough IFIs after prophylaxis with echinocandins in patients receiving new targeted therapies. When antifungals fail to reach adequate levels and invasive aspergillosis is suspected, combining antifungals from different families is deemed appropriate.
Review
Infectious Diseases
Victor Garcia-Bustos, Marta Dafne Cabanero-Navalon, Alba Ruiz-Gaitan, Miguel Salavert, Maria Angeles Tormo-Mas, Javier Peman
Summary: This review summarizes the genetic background, ecological niches, and global dissemination mechanisms of Candida auris, and highlights the potential role of animals in transmission. The research suggests that the emergence of C. auris may be related to global warming, and also discusses the relationship between human impact on the environment and the rise of new fungal pathogenic species.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Critical Care Medicine
Emilio Maseda, Ignacio Martin-Loeches, Rafael Zaragoza, Javier Peman, Jesus Fortun, Santiago Grau, Gerardo Aguilar, Marina Varela, Marcio Borges, Maria-Jose Gimenez, Alejandro Rodriguez