Journal
PROTEOMICS CLINICAL APPLICATIONS
Volume 13, Issue 3, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.201800079
Keywords
early-onset; leucine-rich alpha-2-glycoprotein; myocardial infarction; pregnancy zone protein; proteomic
Funding
- National Natural Science Foundation of China [81672073, 81800238]
- China Postdoctoral Science Foundation [2016M590620]
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Purpose The present study aims to discover novel serum biomarkers of early-onset myocardial infarction (MI) using proteomic analysis. Experimental design In the first stage, the iTRAQ-coupled LC-MS/MS technique is utilized to investigate protein profiles of patients with early-onset MI. In the second stage, these candidate proteins are validated using ELISA. Results A total of 538 proteins are quantified, with pregnancy zone protein (PZP), leucine-rich alpha-2-glycoprotein (LRG) and Apolipoprotein C-I (Apo C-I) being upregulated and Apolipoprotein A-I (Apo A-I) and Apolipoprotein A-IV (Apo A-IV) downregulated in early-onset MI patients. Results from the validation stage demonstrate that the serum concentrations of PZP and LRG are significantly increased in the early-onset MI group. The correlation between the concentrations of C-reactive protein (CRP) and the two candidate biomarkers is positive. Area under the curve values used to diagnose early-onset MI for LRG and PZP are 0.939 and 0.874, respectively. Conclusions and clinical relevance Five differential serum proteins are identified in early-onset MI using proteomic analysis. Lipoprotein-related biomarkers further demonstrate the close relationship between lipid metabolism and the disease. Inflammation-associated LRG and PZP may be novel biomarkers of the disease. In addition, changes in these proteins may partly reveal the possible mechanisms in the pathogenesis and pathophysiology of early-onset MI.
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