Journal
PROCESS BIOCHEMISTRY
Volume 78, Issue -, Pages 71-81Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2019.01.009
Keywords
Co-immobilization; Dextransucrase; Oligosaccharides; Epoxy-agarose; Eupergit CM
Categories
Funding
- CNPq
- National Institute of Tropical Fruits (INCT-FT)
- FUNCAP
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) [001]
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The best support reported for dextransucrase (DS) and dextranase (DN) immobilization was Eupergit C, but this carrier was discontinued and is no longer available in the market. Thus, epoxy-agarose support is suggested as an alternative to co-immobilize DS from Leuconostoc mesenteroides B-512F and DN from Chaetomium erraticum. The co-immobilization approach improved DS performance, compared to immobilized DS, due to the removal of the dextran layer accumulated on DS by DN, and enhanced both enzymes catalytic activities under a wide range of pH and temperature. Since DS and DN have the same optimum pH and temperature, the co-immobilization was done in a single step. The EPA-DS-DN0.5 (co-immobilized DS (26.16 UI) and DN (2.76 UI) in epoxy-agarose) showed the highest recovered activity (59.54%). The biocatalyst was stable at 4 degrees C, retaining above 70% of activity for 60 days and was efficient producing oligosaccharides, yielding 12.68 +/- 0.09 g/L of oligosaccharides with a degree of polymerization up to 5.
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