Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 116, Issue 7, Pages 2577-2582Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1812632116
Keywords
influenza virus; single-particle tracking; quantum dots; uncoating; vRNP dynamics
Categories
Funding
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB29050201]
- National Key Research and Development Program of China [2018ZX10301405]
- National Natural Science Foundation of China [31470269, 21727816, 91743108, 31470837]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences
Ask authors/readers for more resources
Uncoating is an obligatory step in the virus life cycle that serves as an antiviral target. Unfortunately, it is challenging to study viral uncoating due to methodology limitations for detecting this transient and dynamic event. The uncoating of influenza A virus (IAV), which contains an unusual genome of eight segmented RNAs, is particularly poorly understood. Here, by encapsulating quantum dot (QD)-conjugated viral ribonucleoprotein complexes (vRNPs) within infectious IAV virions and applying single-particle imaging, we tracked the uncoating process of individual IAV virions. Approximately 30% of IAV particles were found to undergo uncoating through fusion with late endosomes in the around-nucleus region at 30 to 90 minutes postinfection. Inhibition of viral M2 proton channels and cellular endosome acidification prevented IAV uncoating. IAV vRNPs are released separately into the cytosol after virus uncoating. Then, individual vRNPs undergo a three-stage movement to the cell nucleus and display two diffusion patterns when inside the nucleus. These findings reveal IAV uncoating and vRNP trafficking mechanisms, filling a critical gap in knowledge about influenza viral infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available