Article
Biochemistry & Molecular Biology
Marcos Quintela-Baluja, Kelly Jobling, David W. Graham, Shamas Tabraiz, Burhan Shamurad, Mohamed Alnakip, Karola Bohme, Jorge Barros-Velazquez, Monica Carrera, Pilar Calo-Mata
Summary: This study employed proteomic analysis to rapidly characterize two bacteriocin-producing Enterococcus faecium strains and demonstrated the great potential of this workflow in determining and optimizing the bioengineering and biotechnology properties of other LAB strains in the food industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Samantha Joy D. Valledor, Clarizza May Dioso, Jorge Enrique Vazquez Bucheli, Yu Jin Park, Dong Ho Suh, Eun Sung Jung, Bobae Kim, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: Enterococcus faecium ST20Kc and ST41Kc isolated from kimchi produce bacteriocins with strong activity against Listeria monocytogenes and various Enterococcus spp. The antimicrobials are proteinaceous and stable, with a bactericidal effect on targeted bacterial strains. Both strains are susceptible to antibiotics and potentially produce additional antimicrobial compounds with metabolomics analysis.
Article
Microbiology
Svetoslav Dimitrov Todorov, Richard Weeks, Igor Popov, Bernadette Dora Gombossy de Melo Franco, Michael Leonidas Chikindas
Summary: This study focuses on two strains of lactic acid bacteria that produce bacteriocin-like inhibitor substances (BLIS) active against Candida albicans. The BLIS showed antimicrobial activity against both Listeria monocytogenes and C. albicans. It was found that the adsorption levels of BLIS to C. albicans were influenced by factors such as temperature, pH, and the presence of a contraceptive.
Article
Food Science & Technology
Mengjie Xia, Shuting Mu, Yaowei Fang, Xiaomeng Zhang, Guang Yang, Xiaoyue Hou, Fuxiang He, Yaling Zhao, Yichen Huang, Wei Zhang, Juan Shen, Shu Liu
Summary: The potential application of Enterococcus faecium FUA027 in industrial fermentation for the production of urolithin A was evaluated through genetic and probiotic characteristics analysis. The strain exhibited the ability to transform ellagic acid to urolithin A and showed sensitivity to clinically relevant antibiotics and good antioxidant activity.
Article
Agriculture, Dairy & Animal Science
Ashraf Khalifa, Hairul Islam Mohamed Ibrahim
Summary: In this study, beneficial probiotics, specifically Enterococcus faecium, were isolated from broiler chicken carcasses and found to improve chicken health against Salmonella infections. Chickens fed with E. faecium showed improved body weight, reduced mortality, and lower levels of S. enterica. The presence of E. faecium also led to increased levels of short-chain fatty acids and reduced levels of pro-inflammatory markers. These findings highlight the potential of E. faecium as a preventive measure against bacterial infections in broiler chickens.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
B. S. Sabna, Bency Thankappan, Ramasamy Mahendran, Gayathri Muthusamy, Daniel Raja Femil Selta, Jayaraman Angayarkanni
Summary: This study evaluated the GABA production and probiotic capability of lactic acid bacteria strains isolated from dairy products, identifying Enterococcus faecium BS5 as the most efficient GABA producer. This strain showed resistance to acid stress, bile salt, and antibiotics, making it potentially valuable for large-scale industrial production of GABA and functional fermented product development.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Multidisciplinary Sciences
Nashwa Tarek, Ahmed F. Azmy, Ahmed S. Khairalla, Medhat Abdel-Fattah, Ohoud A. Jefri, Mohamed Shaban, Abdelaziz A. A. El-Sayed, Ahmed O. El-Gendy
Summary: This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. The bacterium NT04 was identified as Enterococcus faecium through 16S rRNA gene sequencing. Genomic analysis confirmed the presence of genes encoding diverse metabolic and probiotic properties in strain NT04. No pathogenicity islands or plasmid insertions were found. The genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.
Article
Pharmacology & Pharmacy
Virginia Barreto Lordello, Andreia Bagliotti Meneguin, Sarah Raquel de Annunzio, Maria Pia Taranto, Marlus Chorilli, Carla Raquel Fontana, Daniela Cardoso Umbelino Cavallini
Summary: The orodispersible film developed in this study successfully delivered probiotic Enterococcus faecium CRL 183 into the oral cavity, demonstrating good antifungal activity against Candida albicans and reducing biofilm formation.
Article
Microbiology
Ingrid Maria Cecilia Rubin, Sarah Mollerup, Christa Broholm, Signe Boye Knudsen, Adam Baker, Morten Helms, Mona Katrine Alberthe Holm, Thomas Kallemose, Henrik Westh, Jenny Dahl Knudsen, Mette Pinholt, Andreas Munk Petersen
Summary: This trial aimed to evaluate the efficacy of Lactobacillus rhamnosus GG (LGG) supplementation for 4 weeks in eliminating the gastrointestinal carrier state of vancomycin-resistant Enterococcus faecium (VREfm) in hospitalized adults. The study found no significant effect of LGG in eradicating VREfm and observed a high natural clearance rate. Microbiome analysis showed no significant difference in diversity between the LGG and placebo groups.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Noha A. Ahmed, Rania Abdelmonem Khattab, Yasser M. Ragab, Mariam Hassan
Summary: This study evaluated the probiotic and safety characteristics of two E. lactis strains isolated from the human gut using in-vitro and in silico approaches. The results showed that the strains exhibited resistance to bile salts and displayed antibacterial activity against certain pathogens. Comparative genome analysis revealed the absence of transferable antibiotic resistance genes. These findings suggest that E. lactis could be a potential source for safer Enterococcus probiotic supplements.
Article
Agriculture, Dairy & Animal Science
Pragathi B. Shridhar, Raghavendra G. Amachawadi, Mike Tokach, Isha Patel, Jayanthi Gangiredla, Mark Mammel, T. G. Nagaraja
Summary: Whole-genome sequencing of Enterococcus faecium strains isolated from commercial swine and cattle probiotics revealed the presence of antimicrobial resistance genes, but absence of major virulence genes. This suggests that these probiotic strains are unlikely to cause opportunistic infection, but they may pose a risk of spreading antimicrobial resistance genes to other gut bacteria.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Abubakar Siddique, Sara Azim, Amjad Ali, Fazal Adnan, Maryum Arif, Muhammad Imran, Erika Ganda, Abdur Rahman
Summary: Enteric infections like Salmonella spp. are common in poultry, and antibiotic resistance is emerging due to continued use of growth-promoting antibiotics. Probiotics from poultry gut can potentially control antibiotic-resistant bacteria. Lactic acid bacteria from poultry gut, like L. reuteri PFS4, E. faecium PFS13, and E. faecium PFS14, show promise as potential probiotics against multi-drug resistant Salmonella Typhimurium and S. Enteritidis. Further research is needed to assess the in vivo potential for controlling Salmonella in poultry and feed.
Article
Agriculture, Dairy & Animal Science
H. H. Azzaz, A. A. Awad, H. A. Murad, Noha A. Hassaan
Summary: The addition of two strains of probiotics to the diet of ewes improved nutrient digestibility, increased blood glucose and protein concentrations, and enhanced milk yield and composition.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Microbiology
Da Hye Kim, Seul-Ah Kim, Na Gyeong Jo, Jae-Han Bae, Minh Tri Nguyen, Yu Mi Jo, Nam Soo Han
Summary: This study provides evidence that the Enterococcus faecium strain EFEL8600, isolated from Korean soy-meju, exhibits probiotic characteristics including inhibitory activity against Listeria monocytogenes, resilience in gastrointestinal conditions, antioxidant and anti-inflammatory activities, and protection of the intestinal barrier.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Elke Albrecht, Rudolf Zitnan, Viera Karaffova, Viera Revajova, Michaela Cechova, Martin Levkut Jr, Monika Roentgen
Summary: The application of the probiotic Enterococcus faecium in broiler chickens has been found to improve the development and tissue properties of the pectoralis major muscle, including increased myonuclei number per fiber, improved capillarization, larger myofibers, and increased body and muscle weight. These effects are possibly related to elevated intramuscular expression of IGF-1 and reduced MYF5 expression.
Article
Microbiology
Graham A. J. Redweik, Karrie Daniels, Andrew J. Severin, Mark Lyte, Melha Mellata
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Psychology, Biological
Nancy K. Dess, Clinton D. Chapman, Farnaz Fouladi, Anthony A. Fodor, Mark Lyte
PHYSIOLOGY & BEHAVIOR
(2020)
Article
Multidisciplinary Sciences
Meicen Liu, Mark Lyte
Article
Nutrition & Dietetics
Danielle R. Reed, Amber L. Alhadeff, Gary K. Beauchamp, Nirupa Chaudhari, Valerie B. Duffy, Monica Dus, Alfredo Fontanini, John Glendinning, Barry G. Green, Paule Joseph, George A. Kyriazis, Mark Lyte, Padma Maruvada, John P. McGann, John T. McLaughlin, Timothy H. Moran, Claire Murphy, Emily E. Noble, M. Yanina Pepino, Jennifer L. Pluznick, Kristina Rother, Enrique Saez, Alan C. Spector, Catia Sternini, Richard D. Mattes
Summary: The workshop discussed the need to optimize human chemosensory testing and assessment, the plasticity of chemosensory systems, and the interplay of chemosensory signals, cognitive signals, dietary intake, and metabolism. Research priorities identified included refining methods for measuring chemosensation in large cohort studies, characterizing interindividual differences in chemosensory function, defining circuit-level organization and function, and discovering new ligands for chemosensory receptors. The COVID-19 pandemic has highlighted the urgent need to understand the effects of the virus on flavor perception and the potential long-term implications for health and nutrition policy.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2021)
Article
Agriculture, Dairy & Animal Science
Joshua M. Lyte, Sandip Shrestha, Basanta R. Wagle, Rohana Liyanage, Diego A. Martinez, Annie M. Donoghue, Karrie M. Daniels, Mark Lyte
Summary: Microbial endocrinology research has shown that neurochemicals, such as serotonin, may impact the colonization of bacteria like Campylobacter jejuni in the gut. Further studies are needed to understand the specific effects and potential applications of serotonin in controlling bacterial colonization in vivo.
Article
Substance Abuse
Ella E. Bauer, Allyse Shoeman, Trevor J. Buhr, Karrie M. Daniels, Mark Lyte, Peter J. Clark
Summary: The study found that alcohol abuse has an impact on gut monoamine-related neurochemical concentrations, primarily affecting dopamine activity; there is a correlation between neurochemical patterns in the small intestine and the striatum, indicating modulatory activity between these areas; binge drinking influences monoaminergic activity in the hypothalamic region, in addition to brain areas in the mesolimbic dopamine system. Sex differences were observed in gut neurochemical concentrations, particularly in the small intestine.
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
(2021)
Article
Food Science & Technology
Jordi Mayneris-Perxachs, Wellington Amaral, Gabriele R. Lubach, Mark Lyte, Gregory J. Phillips, Joram M. Posma, Christopher L. Coe, Jonathan R. Swann
Summary: Infants worldwide are seriously affected by iron deficiency, but the lingering metabolic and microbial effects after natural recovery have been found in juvenile monkeys, while early treatment with iron dextran and B vitamins can prevent these long-term biochemical derangements.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Microbiology
Joshua M. Lyte, James Keane, Julia Eckenberger, Nicholas Anthony, Sandip Shrestha, Daya Marasini, Karrie M. Daniels, Valentina Caputi, Annie M. Donoghue, Mark Lyte
Summary: This study reveals significant differences in cecal microbial community structure, tissue neurochemical concentrations, and gut structural morphologies between birds with different corticosterone responses to stress. Unique neurochemical changes in the intestine following stress suggest a potential relationship between the cecal microbiome and overall avian physiology.
Article
Biochemistry & Molecular Biology
Danielle N. Rendina, Gabriele R. Lubach, Mark Lyte, Gregory J. Phillips, Ankush Gosain, Joseph F. Pierre, Roza M. Vlasova, Martin A. Styner, Christopher L. Coe
Summary: This study tracked the assembly of gut microbiota during the initial nursing period and transition to solid food in infant monkeys. The dynamic bacterial community structure reflected different maturational phases, influenced early by breast milk and later by solid foods. Higher abundance of certain microbial taxa during nursing was associated with slower growth trajectories and smaller brain volumes at one year of age.
Article
Surgery
Joseph F. Pierre, Greg J. Phillips, Lawrance C. Chandra, Danielle N. Rendina, Neena F. Thomas-Gosain, Gabriele R. Lubach, Mark Lyte, Christopher L. Coe, Ankush Gosain
Summary: The study found that inclusion of lyticase in the sample preparation process significantly increased fungal ITS reads, enhanced detection of numerous fungal genera in non-human primate fecal samples, and maintained fidelity of microbiome identification and community features.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Margie D. Lee, Ignacio R. Ipharraguerre, Ryan J. Arsenault, Mark Lyte, Joshua M. Lyte, Brooke Humphrey, Roselina Angel, Douglas R. Korver
Summary: This paper is a compilation of talks given by leading international researchers in the field of poultry gut microbiology and microbiome research. The talks cover various aspects such as historical developments, the impact of the microbiome on health, and the mechanisms of host-microbiome interactions, emphasizing the importance of a deeper understanding of host microbiomes.
Article
Biology
Graham A. J. Redweik, Michael H. Kogut, Ryan J. Arsenault, Mark Lyte, Melha Mellata
Summary: The study demonstrates that reserpine enhances Salmonella resistance in the chicken intestine by modulating neuro-immunometabolic signaling, leading to reduced bacterial colonization and improved food safety.
COMMUNICATIONS BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Joshua M. Lyte, Diego A. Martinez, Kelsy Robinson, Annie M. Donoghue, Karrie M. Daniels, Mark Lyte
Summary: Microbial endocrinology is the study of neurochemical-based inter kingdom signaling and its impact on host-microbe interaction. Neurochemicals play a crucial role in determining bacterial colonization and interaction with the gut epithelium. However, little is known about the biogeography of neurochemicals in the avian gut. This study aimed to determine the concentrations of stress-related neurochemicals in different regions of the broiler chicken intestinal tract and investigate changes with age. The results showed regional differences in neurochemical concentrations and age-related changes for certain neurochemicals.
Article
Microbiology
Edward Fox, Mark Lyte
Summary: This study reveals the anatomical relationship between microbes and mucosal elements in the gut, showing that the majority of microbiota are associated with food. It suggests that microbiota may potentially influence behavior through the secretion of bacterial products that interact with gut neurons.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Lucas R. Koester, Kris Hayman, Chiron J. Anderson, Bienvenido W. Tibbs-Cortes, Karrie M. Daniels, Faith M. Seggerman, Patrick J. Gorden, Mark Lyte, Stephan Schmitz-Esser
Summary: Mitigating the effects of heat stress on livestock is crucial for their health and performance. One method is to enhance feed palatability by adding artificial sweeteners, although their impact on the rumen microbial communities is not fully understood. This study found that supplementing dairy cattle's diet with a saccharin-based sweetener did not affect their performance during heat stress, but it did lead to changes in the rumen microbial communities. Further research is needed to fully understand the role of artificial sweeteners in mitigating stress conditions for livestock and their effects on microbial communities.
JOURNAL OF ANIMAL SCIENCE
(2023)