Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection

Phase 1 clinical study of an embryonic stem cell–derived retinal pigment epithelium patch in age-related macular degeneration

(2018) Lyndon da Cruz et al.   NATURE BIOTECHNOLOGY

A bioengineered retinal pigment epithelial monolayer for advanced, dry age-related macular degeneration

(2018) Amir H. Kashani et al.   Science Translational Medicine

Generation of a rod-specific NRL reporter line in human pluripotent stem cells

(2018) M. Joseph Phillips et al.   Scientific Reports

Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection

(2018) Benjamin Steyer et al.   Stem Cell Reports

‘Cold shock’ increases the frequency of homology directed repair gene editing in induced pluripotent stem cells

(2018) Q. Guo et al.   Scientific Reports

Autologous Induced Stem-Cell–Derived Retinal Cells for Macular Degeneration

(2017) Michiko Mandai et al.   NEW ENGLAND JOURNAL OF MEDICINE

Hit and go CAS9 delivered through a lentiviral based self-limiting circuit

(2017) Gianluca Petris et al.   Nature Communications

Enhanced Stem Cell Differentiation and Immunopurification of Genome Engineered Human Retinal Ganglion Cells

(2017) Valentin M. Sluch et al.   Stem Cells Translational Medicine

Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection

(2017) Katie A. Mitzelfelt et al.   Stem Cell Reports

Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells

(2017) Karl J. Wahlin et al.   Scientific Reports

Parkinson-associated risk variant in distal enhancer of α-synuclein modulates target gene expression

(2016) Frank Soldner et al.   NATURE

Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies

(2016) Gang Wang et al.   Nature Protocols

Induced pluripotent stem cell technology: a decade of progress

(2016) Yanhong Shi et al.   NATURE REVIEWS DRUG DISCOVERY

Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair

(2016) Xiangjun He et al.   NUCLEIC ACIDS RESEARCH

Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells

(2016) Brian R. Shy et al.   NUCLEIC ACIDS RESEARCH

CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice

(2016) Asami Oji et al.   Scientific Reports

Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis

(2016) Manuela Völkner et al.   Stem Cell Reports

Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9

(2015) Yuejun Chen et al.   Cell Stem Cell

Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells

(2015) Chen Yu et al.   Cell Stem Cell

Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy: follow-up of two open-label phase 1/2 studies

(2015) Steven D Schwartz et al.   LANCET

Small-molecule–directed, efficient generation of retinal pigment epithelium from human pluripotent stem cells

(2015) Julien Maruotti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus

(2015) Florian T. Merkle et al.   Cell Reports

A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells

(2015) Zengrong Zhu et al.   Stem Cell Reports

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

(2015) Manuel Stemmer et al.   PLoS One

Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line

(2015) Valentin M. Sluch et al.   Scientific Reports

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells

(2014) Federico González et al.   Cell Stem Cell

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System

(2014) Kai Li et al.   PLoS One

Dual ReporterMESP1mCherry/w-NKX2-5eGFP/whESCs Enable Studying Early Human Cardiac Differentiation

(2014) Sabine C. Den Hartogh et al.   STEM CELLS

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

Transplantation of Embryonic and Induced Pluripotent Stem Cell-Derived 3D Retinal Sheets into Retinal Degenerative Mice

(2014) Juthaporn Assawachananont et al.   Stem Cell Reports

Kinetics of drug selection systems in mouse embryonic stem cells

(2013) Yuhki Nakatake et al.   BMC BIOTECHNOLOGY

Enhanced Efficiency of Human Pluripotent Stem Cell Genome Editing through Replacing TALENs with CRISPRs

(2013) Qiurong Ding et al.   Cell Stem Cell

LHX2 Is Necessary for the Maintenance of Optic Identity and for the Progression of Optic Morphogenesis

(2013) A. Roy et al.   JOURNAL OF NEUROSCIENCE

Optimization of scarless human stem cell genome editing

(2013) Luhan Yang et al.   NUCLEIC ACIDS RESEARCH

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models

(2012) Qiurong Ding et al.   Cell Stem Cell

Short DNA sequences inserted for gene targeting can accidentally interfere with off-target gene expression

(2010) Ingo D. Meier et al.   FASEB JOURNAL

The LIM homeobox transcription factor Lhx2 is required to specify the retina field and synergistically cooperates with Pax6 for Six6 trans-activation

(2008) Nicolas Tétreault et al.   DEVELOPMENTAL BIOLOGY