Article
Cell & Tissue Engineering
Jr. Ferreira, G. Q. Teixeira, E. Neto, C. Ribeiro-Machado, A. M. Silva, J. Caldeira, C. Leite Pereira, S. Bidarra, A. F. Maia, M. Lamghari, M. M. Barbosa, R. M. Goncalves
Summary: This study showed that MSC secretome can reduce pro-inflammatory gene expression in degenerated IVDs, promote matrix degrading enzyme expression, and increase aggrecan deposition. Additionally, the secretome had a strong immunomodulatory role in the degenerated IVD, making it a potential therapeutic approach for low-back pain.
EUROPEAN CELLS & MATERIALS
(2021)
Review
Immunology
Sylwia Dabrowska, Anna Andrzejewska, Miroslaw Janowski, Barbara Lukomska
Summary: Mesenchymal stem cells (MSCs) possess supportive, differentiating, and immunoregulatory functions, while the extracellular vesicles (EVs) they produce can serve as a potential cellular therapy tool.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Benjamin Leon-Mancilla, Moises Martinez-Castillo, Zaira Medina-Avila, Armando Perez-Torres, Jorge Garcia-Loya, Ana Alfaro-Cruz, Cristina Pina-Barba, Gabriela Gutierrez-Reyes
Summary: Liver diseases are a leading cause of death worldwide, with liver transplantation being the recommended procedure for advanced patients. Extracellular matrix scaffolds have emerged as an alternative for tissue restoration, offering benefits such as biocompatibility and graft acceptance. Xenoimplantation of a collagen matrix scaffold in a rat liver showed promising results in liver tissue repair and regeneration.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Materials Science, Biomaterials
Xiufang Li, Renchuan You, Qiang Zhang, Shuqin Yan, Zuwei Luo, Jing Qu, Mingzhong Li
Summary: The research aims to improve cell transplantation efficacy by utilizing scaffolds with nanofibrous microstructure for cell colonization and vascularization. Experimental results demonstrate that multiscale hierarchical design significantly enhances the ability of transplanted cells to promote neovascularization and tissue reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Materials Science, Ceramics
Patricia Ros-Tarraga, Carlos M. Martinez, Miguel A. Rodriguez, Piedad N. De Aza
Summary: The elderly population is increasing, leading to an increase in bone and joint problems. Scaffold for bone tissue regeneration can solve these problems, and material selection is crucial for satisfactory results. This study designed a three-dimensional porous ceramic scaffold and analyzed its biocompatibility and osteoinductive capacity. The results suggest that it has potential applications as bone substitutes.
CERAMICS INTERNATIONAL
(2022)
Review
Allergy
Ming Wang, Ning Zhao, Chengshuo Wang, Zi-Bing Jin, Luo Zhang
Summary: Allergic rhinitis is a common chronic inflammatory disorder that significantly impacts patients' health and quality of life. Current treatments for allergic rhinitis are limited in their ability to restore immune balance and target specific allergens. Mesenchymal stem cells (MSCs) offer a potential therapeutic strategy for allergic rhinitis due to their immune privilege and strong immunomodulatory effects. Recent studies have investigated the use of MSCs in animal models of allergic rhinitis and have shown promising results. This review discusses the immunomodulatory effects of MSCs on allergic airway inflammation, highlights recent research on the role of MSCs in modulating immune cells, and explores the clinical potential of MSC-based therapy for allergic rhinitis.
Article
Engineering, Multidisciplinary
Yaling Yu, Weiying Zhang, Xuanzhe Liu, Hongshu Wang, Junjie Shen, Huimin Xiao, Jin Mei, Yimin Chai, Gen Wen
Summary: Decellularized biomaterials have shown great potential in regenerative medicine, with good biocompatibility and interaction with the immune system being important factors to consider. Understanding the immunogenicity and immunomodulatory effects of decellularized scaffolds on innate and adaptive immune systems is crucial for their clinical translation. Host responses mediated by decellularized biomaterials can provide insights for further development and application in vivo.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Alireza Yeganeh, Anwar Fathollahi, Seyed Mahmoud Hashemi, Farshid Yeganeh
Summary: The study evaluated the effect of mesenchymal stem cells-derived extracellular vesicles (MSC-EVs) on cytokine production and gene expression related to T helper cell subsets. The treatment with MSC-EV increased the frequency of CD4(+)CD25(+)FOXP3(+) T cells and upregulated Foxp3 mRNA level.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Cell Biology
Chunxue Zhang, Liqiang Zhou, Zhen Wang, Wenxia Gao, Wei Chen, Huina Zhang, Bo Jing, Xu Zhu, Lei Chen, Changhong Zheng, Kaiyan Shi, Li Wu, Liming Cheng, Kunshan Zhang, Yi Eve Sun
Summary: This study revealed variations in cell proliferation and immune modulatory functions of MSCs from different donors, with IFN-gamma and NF-kappa B signaling being positively associated with immune modulatory function. Activation of these pathways could eradicate donor-dependent variations, but overall transcriptomic differences between donor-derived MSCs still exist.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Hao Cai, Haidong Guo
Summary: Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic tool for various diseases due to their ability to differentiate into multiple cell types and their secreted factors. In recent years, the extracellular vesicles (EVs) derived from MSCs have been shown to possess similar therapeutic functions as MSCs. This review provides an overview of the research progress in MSCs and their EVs in different disease conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Huynh-Quang-Dieu Nguyen, Chen-Yu Kao, Chien-Ping Chiang, Yu-Han Hung, Chun-Min Lo
Summary: This study investigates the immunomodulatory properties of extracellular matrix (ECM) and mesenchymal stem cells (MSCs). The results show that degradation products from porcine bladder ECM can reduce inflammation and increase the production of anti-inflammatory cytokines from macrophages. MSCs cultured on the ECM hydrogel demonstrate immunomodulatory properties at both gene and protein levels. The findings provide a basis for further research on immunomodulation therapy.
Article
Engineering, Biomedical
Guangzhao Tian, Shuangpeng Jiang, Junqi Li, Fu Wei, Xu Li, Yi Ding, Zhen Yang, Zhiqiang Sun, Kangkang Zha, FuXin Wang, Bo Huang, Liqing Peng, Qiuming Wang, Zhuang Tian, Xi Yang, Zhigang Wang, Quanyi Guo, Weimin Guo, Shuyun Liu
Summary: This study demonstrates that pepsin-solubilized DCM (PDCM) can activate bone marrow-derived macrophages to polarize towards a beneficial macrophage phenotype. These polarized macrophages promote bone marrow-derived mesenchymal stem cell invasion, migration, proliferation, and chondrogenic differentiation. Combining DCM scaffolds with early intra-articular injection of IL-4 creates a wound-healing microenvironment that enhances cartilage regeneration in a rat knee osteochondral defect model.
ACTA BIOMATERIALIA
(2021)
Article
Nanoscience & Nanotechnology
Baojie Guo, Yongchao Duan, Zhongwen Li, Yao Tian, Xuedi Cheng, Chunxu Liang, Wenjing Liu, Bin An, Wumei Wei, Tingting Gao, Shasha Liu, Xiyuan Zhao, Shuaishuai Niu, Chenxin Wang, Yukai Wang, Liu Wang, Guihai Feng, Wei Li, Jie Hao, Qi Gu, Qi Zhou, Jun Wu
Summary: The researchers developed a 21-day long-term serum-free culture system and successfully created cell sheets (CSs) with human embryonic stem cell-derived immunity-and-matrix-regulatory cells (IMRCs) that have stronger mechanical properties and greater extracellular matrix secretion capacity. These IMRC-CSs were effective in promoting corneal chemical injury repair and could be transformed into injectable temperature-sensitive hydrogels for uterine adhesion repair through a decellularization process.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Pharmacology & Pharmacy
Rui Alvites, Mariana Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Ana Colette Mauricio
Summary: Mesenchymal Stem/Stromal Cells (MSCs) have high clinical value and have been extensively studied. Their immunomodulatory influence occurs through both direct and paracrine routes, but the mechanisms are not fully understood. This review summarizes the immunoregulatory capacity of MSCs and their secretion products, with a focus on migration, homing, direct cell action, and paracrine activity, and explores related techniques.
Review
Cell Biology
Ekaterina Sergeevna Novoseletskaya, Pavel Vladimirovich Evdokimov, Anastasia Yurievna Efimenko
Summary: The extracellular matrix plays a crucial role in regulating cell behavior and fate in stem cell microenvironments. Different types of stem cells possess molecules that interact with the extracellular matrix and cause changes in gene expression. Signals from the extracellular matrix are important for embryonic and postnatal development, as well as tissue renewal and repair. Various methods are used to study the cellular signaling networks from the extracellular matrix and predict the pathways in target cells.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Veterinary Sciences
G. A. Lake-Bakaar, L. G. Griffiths, M. D. Kittleson
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2017)
Article
Engineering, Biomedical
Cai Li, Jeny Shklover, Mojtaba Parvizi, Benjamin E. Sherlock, Alba Alfonso Garcia, Anne K. Haudenschild, Leigh G. Griffiths, Laura Marcu
ANNALS OF BIOMEDICAL ENGINEERING
(2018)
Article
Immunology
Katherine V. Gates, Leigh G. Griffiths
IMMUNOLOGIC RESEARCH
(2018)
Article
Biochemical Research Methods
Alba Alfonso-Garcia, Jeny Shklover, Benjamin E. Sherlock, Alyssa Panitch, Leigh G. Griffiths, Laura Marcu
JOURNAL OF BIOPHOTONICS
(2018)
Article
Multidisciplinary Sciences
Bence Hegyi, Julie Bossuyt, Leigh G. Griffiths, Rafael Shimkunas, Zana Coulibaly, Zhong Jian, Kristin N. Grimsrud, Claus S. Sondergaard, Kenneth S. Ginsburg, Nipavan Chiamvimonvat, Luiz Belardinelli, Andras Varro, Julius G. Papp, Piero Pollesello, Jouko Levijoki, Leighton T. Izu, W. Douglas Boyd, Tamas Banyasz, Donald M. Bers, Ye Chen-Izu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Engineering, Biomedical
Ailsa J. Dalgliesh, Mojtaba Parvizi, Manuela Lopera-Higuita, Jeny Shklover, Leigh G. Griffiths
ACTA BIOMATERIALIA
(2018)
Article
Biochemical Research Methods
Katherine V. Gates, Qi. Xing, Leigh G. Griffiths
PROTEOMICS CLINICAL APPLICATIONS
(2019)
Article
Multidisciplinary Sciences
Ailsa J. Dalgliesh, Mojtaba Parvizi, Christopher Noble, Leigh G. Griffiths
Article
Chemistry, Analytical
Cai Li, Alba Alfonso-Garcia, James McMasters, Julien Bec, Brent Weyers, Lauren Uyesaka, Leigh Griffiths, Alyssa Panitch, Laura Marcu
METHODS AND APPLICATIONS IN FLUORESCENCE
(2019)
Article
Cell & Tissue Engineering
Manuela Lopera Higuita, Leigh G. Griffiths
TISSUE ENGINEERING PART B-REVIEWS
(2020)
Article
Multidisciplinary Sciences
Jeny Shklover, James McMasters, Alba Alfonso-Garcia, Manuela Lopera Higuita, Alyssa Panitch, Laura Marcu, Leigh Griffiths
SCIENTIFIC REPORTS
(2019)
Article
Engineering, Biomedical
Higuita Manuela Lopera, Leigh G. Griffiths
ACTA BIOMATERIALIA
(2020)
Article
Chemistry, Analytical
Tanveer Ahmed Shaik, Alba Alfonso-Garcia, Xiangnan Zhou, Katherine M. Arnold, Anne K. Haudenschild, Christoph Krafft, Leigh G. Griffiths, Juergen Popp, Laura Marcu
ANALYTICAL CHEMISTRY
(2020)
Article
Cell Biology
Jingjing Wu, Hyunwoo Yuk, Tiffany L. Sarrafian, Chuan Fei Guo, Leigh G. Griffiths, Christoph S. Nabzdyk, Xuanhe Zhao
Summary: This study introduces an off-the-shelf bioadhesive gastrointestinal (GI) patch that can rapidly and robustly repair GI defects without the need for sutures. The patch has been shown to be biocompatible and degradable, and has demonstrated successful sealing and healing of GI defects in animal models.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Review
Cell & Tissue Engineering
Likitha Somasekhar, Leigh G. Griffiths
Summary: Whole organ tissue engineering encompasses a variety of approaches, including 3D printed tissues, cell-based self-assembly, and cellular incorporation into synthetic or xenogeneic extracellular matrix (ECM) scaffolds. This review article addresses the importance of whole organ tissue engineering for various solid organ applications, focusing on the use of extracellular (ECM) matrix scaffolds in such engineering endeavors. This work highlights the emerging barriers and potential solutions for ECM scaffold-based tissue-engineered organs, including recapitulation of a functional vascular tree, delivery and orientation of cells into parenchymal void spaces, and driving differentiation of delivered cells toward the appropriate lineage. The insights presented will maximize the potential of allogeneic or xenogeneic ECM scaffolds in future whole organ tissue-engineering efforts.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)