Article
Biochemistry & Molecular Biology
Hiroshi Maruoka, Tomohito Tanaka, Hikaru Murakami, Hiromitsu Tsuchihashi, Akihiko Toji, Misa Nunode, Atsushi Daimon, Shunsuke Miyamoto, Ruri Nishie, Shoko Ueda, Sousuke Hashida, Shinichi Terada, Hiromi Konishi, Yuhei Kogata, Kohei Taniguchi, Kazumasa Komura, Masahide Ohmichi
Summary: This study identified cancer-specific miRNAs in tissue-exudative extracellular vesicles (Te-EVs) of ovarian clear cell carcinomas (OCCs). The levels of these miRNAs were significantly decreased in postoperative serum samples, indicating their potential as biomarkers for the early diagnosis and detection of OCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Yukang Lu, Mengting Zhang, Jiajun Zhou, Xiulan Liu, Lanfeng Wang, Xinyi Hu, Yiping Mao, Rongfa Gan, Zhiping Chen
Summary: This review provides an overview of recent studies on the role of extracellular vesicles (EVs) in the progression of renal cell carcinoma (RCC), including their involvement in tumor cell interaction with M2 macrophages, mediating immune tolerance, and acting as communication messengers in the tumor microenvironment. The review also discusses the potential use of engineered sEVs or EV tumor vaccines, mesenchymal stem cell EV therapy, and reduction of tumor-derived EV secretion in RCC treatment. Overall, the review highlights the importance of EVs in RCC and their potential as a pathway for future treatment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Corinna Steidel, Fanny Ender, Achim Rody, Nikolas von Bubnoff, Frank Gieseler
Summary: This study discusses the potential causal role of tissue factor-bearing extracellular vesicles (TF+ EVs) released from tumor cells in the pathological coagulation status of ovarian cancer patients. Results show that EVs derived from ascites of ovarian cancer patients have significantly high TF activities and are associated with a high rate of VTE, suggesting a potential role in prognosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tanja Eichhorn, Rene Weiss, Silke Huber, Marie Ebeyer-Masotta, Marwa Mostageer, Robert Emprechtinger, Ludwig Knabl Sr, Ludwig Knabl, Reinhard Wuerzner, Viktoria Weber
Summary: Patients with severe COVID-19 have increased platelet activation and platelet-leukocyte aggregate formation, resulting in elevated levels of phosphatidylserine (PS)-exposing extracellular vesicles (EVs) and increased tissue factor (TF) expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Su-Ran Li, Qi-Wen Man, Xin Gao, Hao Lin, Jing Wang, Fu-Chuan Su, Han-Qi Wang, Lin-Lin Bu, Bing Liu, Gang Chen
Summary: Extracellular vesicles (EVs) are lipid-bilayer membrane structures secreted by most cell types, acting as messengers through the transfer of lipids, proteins, and nucleic acids to influence cellular processes. Tissue-derived EVs (Ti-EVs) offer advantages such as tissue specificity and accurate reflection of the tissue microenvironment, playing important roles in intercellular communication and disease development insights. Current research on Ti-EVs is limited compared to body fluid or cell culture-derived EVs, highlighting the need for further exploration in basic and clinical applications.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Cell Biology
Liwei Meng, Zhaoquan Xing, Zhaoxin Guo, Yue Qiu, Zhaoxu Liu
Summary: In RCC, elevated levels of miR-155 in EVs were found in patients compared to healthy controls, with hypoxia-induced EVs showing higher levels of miR-155 and promoting cell proliferation. MiR-155 was identified as targeting FOXO3 in RCC cells, and hypoxia-induced EVs were able to significantly inhibit FOXO3 activation through miR-155 up-regulation, thereby enhancing cellular tumor progression.
Review
Oncology
Jose Pedro Sequeira, Vera Constancio, Joao Lobo, Rui Henrique, Carmen Jeronimo
Summary: Liquid biopsies have emerged as a new tool for early diagnosis, with circulating or exosome miRNAs showing great potential in renal cell carcinoma. The study of these miRNAs in liquid biopsies has experienced exponential growth, signaling a promising development in clinical applications.
Review
Biochemistry & Molecular Biology
Zhengke Zhi, Qiaochu Sun, Weibing Tang
Summary: Extracellular vesicles (EV) are vesicular structures with phospholipid bilayer, found in biological fluids and the extracellular microenvironment. They play a crucial role in intercellular communication by delivering lipids, proteins, and RNAs to recipient cells. Tissue derived extracellular vesicles (Ti-EVs), distinct from those derived from biofluids and cell culture, provide us with more accurate and enriched information about the tissue microenvironment, participating directly in the crosstalk between various cell types within the microenvironment. The study of Ti-EVs is increasing as they have the potential to accurately reflect the occurrence and development of human diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Xingyi Chen, Baolong Liu, Xingzhi Li, Thuy T. An, You Zhou, Gang Li, Judy Wu-Smart, Sophie Alvarez, Michael J. Naldrett, James Eudy, Gregory Kubik, Richard A. Wilson, Stephen D. Kachman, Juan Cui, Jiujiu Yu
Summary: Honey has been used as a traditional medicinal food and modern research has discovered its anti-inflammatory properties, as well as a new bioactive component that can alleviate inflammation and liver damage.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Oncology
Kate Glennon, Mahafarin Maralani, Narges Abdian, Antoine Paccard, Laura Montermini, Alice Jisoo Nam, Madeleine Arseneault, Alfredo Staffa, Pouria Jandaghi, Brian Meehan, Fadi Brimo, Simon Tanguay, Janusz Rak, Yasser Riazalhosseini
Summary: Renal cell carcinoma (RCC) poses challenges in identifying high-risk patients for relapse or metastasis due to lack of reliable clinical biomarkers. Our study developed an improved assay to capture prognostic genomic biomarkers in RCC ctDNA, showing reliable performance in detecting gene mutations in tumor DNA isolated from plasma and extracellular vesicles. This study presents an RCC-appropriate sequencing assay and workflow for ctDNA analysis, demonstrating the feasibility of detecting tumor-specific mutations in liquid biopsy in RCC patients.
Review
Immunology
Rui Rui, Liqun Zhou, Shiming He
Summary: This review provides a comprehensive summary of the advancements in exosome research in the context of renal cell carcinoma, emphasizing the importance of exosomes as tumor markers and therapeutic targets.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Cell Biology
Xinyi Hu, Yukang Lu, Jiajun Zhou, Lanfeng Wang, Mengting Zhang, Yiping Mao, Zhiping Chen
Summary: Colorectal cancer is a prevalent malignant tumor with high mortality and incidence rates. Small extracellular vesicles (sEVs) play a significant role in the communication and progression of cancer cells. Regulatory RNAs have emerged as potential biomarkers for the diagnosis and prognosis of colorectal cancer, influencing various cancer-related processes. This review highlights the importance of sEVs as biomarkers in colorectal cancer diagnosis and suggests their potential application in treatment, emphasizing the need for further research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Pathology
Bo Qin, Xi-min Hu, Zhen-hong Su, Xiao-bo Zeng, Hong-ying Ma, Kun Xiong
Summary: Extracellular vesicles (EVs) are structures released by cells into the environment, and studies on directly isolating EVs from tissues are limited. Such studies may provide us with better information.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Chemistry, Multidisciplinary
Shaowei Guo, Lior Debbi, Barak Zohar, Roee Samuel, Roni S. Arzi, Adina Fried, Tahel Carmon, Dudi Shevach, Idan Redenski, Inbar Schlachet, Alejandro Sosnik, Shulamit Levenberg
Summary: Applying mechanical stimuli in bioreactors significantly enhances EV production from dental pulp stem cells. Further mechanistic investigation reveals that this process is mediated by the mechanosensitivity of yes-associated protein (YAP). EVs from mechanically stimulated cells display superior capability in inducing axonal sprouting.
Article
Cell Biology
Kyong-Su Park, Kristina Svennerholm, Rossella Crescitelli, Cecilia Lasser, Inta Gribonika, Jan Lotvall
Summary: Bacterial outer membrane vesicles (OMV) have potential as a new cancer vaccine platform, but they can cause severe toxicity by activating the innate immune system. Synthetic Bacterial Vesicles (SyBV) were produced as an alternative to OMV, showing similar effectiveness in activating dendritic cells but without inducing toxic responses. By combining SyBV with tumor extracellular vesicles, anti-tumor immunity and tumor regression were achieved in melanoma-bearing mice, with a synergistic enhancement when combined with anti-PD-1 inhibitor.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Oncology
Koji Hatano, Tohru Yoneyama, Shingo Hatakeyama, Eisuke Tomiyama, Mutsumi Tsuchiya, Mitsuhisa Nishimoto, Kazuhiro Yoshimura, Eiji Miyoshi, Hirotsugu Uemura, Chikara Ohyama, Norio Nonomura, Kazutoshi Fujita
Summary: By simultaneously analyzing α2,3-Sia-PSA and α1,6-Fuc-PSA, the SF index can effectively differentiate HGPC, providing valuable information for decision making on prostate biopsy in men with abnormal PSA levels.
BRITISH JOURNAL OF CANCER
(2022)
Article
Oncology
Takeshi Ujike, Motohide Uemura, Taigo Kato, Koji Hatano, Atsunari Kawashima, Akira Nagahara, Kazutoshi Fujita, Ryoichi Imamura, Norio Nonomura
Summary: This study assessed the diagnostic validity of a CAD system for bone scans in RCC patients and developed a novel diagnostic model based on the combination of ANN values and ICTP. The model showed high accuracy in detecting bone metastases.
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Oncology
Saizo Fujmoto, Kazutoshi Fujita, Mitsuhisa Nishimoto, Mamoru Hamaguchi, Ken Kuwahara, Mamoru Hashimoto, Shogo Adomi, Takafumi Minami, Masahiro Nozawa, Kazuhiro Yoshimura, Hirotsugu Uemura
Summary: Switching from enzalutamide or apalutamide to darolutamide may be an effective therapeutic option for patients with non-metastatic castration-resistant prostate cancer. This retrospective study demonstrated that approximately 55.5% of patients had a PSA decline >50% after switching to darolutamide, with an average PSA decline of 73%.
Article
Oncology
Mamoru Hashimoto, Kazutoshi Fujita, Eisuke Tomiyama, Saizo Fujimoto, Shogo Adomi, Eri Banno, Takafumi Minami, Tetsuya Takao, Masahiro Nozawa, Hiroaki Fushimi, Kazuhiro Yoshimura, Norio Nonomura, Hirotsugu Uemura
Summary: This study investigated the relationship between nectin-4 and HER2 and EGFR in patients with upper urinary tract urothelial carcinoma (UTUC). The results showed high expression of HER2, EGFR, and nectin-4 in UTUC patients. Combination targeted therapy for HER2, EGFR, and nectin-4 may be an effective treatment option for UTUC patients.
ANTICANCER RESEARCH
(2023)
Article
Oncology
Toshiki Oka, Koji Hatano, Yohei Okuda, Akinaru Yamamoto, Toshihiro Uemura, Gaku Yamamichi, Eisuke Tomiyama, Yu Ishizuya, Yoshiyuki Yamamoto, Taigo Kato, Atsunari Kawashima, Kazutoshi Fujita, Norio Nonomura
Summary: This study analyzed patients with castration-resistant prostate cancer (CRPC) who received enzalutamide as first-line therapy. The results showed that lymph node metastasis and time to CRPC can predict the PSA response rate and PSA-progression-free survival of patients treated with enzalutamide.
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Urology & Nephrology
Satoshi Katayama, Benjamin Pradere, Nico C. Grossman, Aaron M. Potretzke, Stephen A. Boorjian, Alireza Ghoreifi, Sia Daneshmand, Hooman Djaladat, John P. Sfakianos, Andrea Mari, Zine-Eddine Khene, David D'Andrea, Nozomi Hayakawa, Alberto Breda, Matteo Fontana, Kazutoshi Fujita, Alessandro Antonelli, Thomas van Doeveren, Christina Steinbach, Keiichiro Mori, Ekaterina Laukhtina, Morgan Roupret, Vitaly Margulis, Pierre Karakiewicz, Motoo Araki, Eva Comperat, Yasutomo Nasu, Shahrokh F. Shariat
Summary: The technical limitations of ureteroscopic biopsy are believed to contribute to the high upgrading rate in upper tract urothelial carcinoma (UTUC). However, the impact of tumor specific factors on upgrading has not been investigated. This study found that high-grade and low-grade biopsy results on ureteroscopy are associated with different biological and clinical features of UTUC tumors.
INTERNATIONAL JOURNAL OF UROLOGY
(2023)
Article
Urology & Nephrology
Maximilian Pallauf, David D'Andrea, Frederik Koenig, Ekaterina Laukhtina, Takafumi Yanagisawa, Morgan Roupret, Siamak Daneshmand, Hooman Djaladat, Alireza Ghoreifi, Francesco Soria, Kazutoshi Fujita, Stephen A. Boorjian, Aaron M. Potretzke, Andrea Mari, Mathieu Roumiguie, Alessandro Antonelli, Alberto Bianchi, Zine-Eddine Khene, John P. Sfakianos, Marcus Jamil, Joost L. Boormans, Jay D. Raman, Nico C. Grossmann, Alberto Breda, Axel Heidenreich, Francesco Del Giudice, Nirmish Singla, Shahrokh F. Shariat, Benjamin Pradere
Summary: This study evaluated the diagnostic accuracy of conventional cross-sectional imaging for lymph node staging in upper tract urothelial cancer and found low sensitivity. Therefore, lymph node dissection should be performed during surgery to obtain accurate staging information.
JOURNAL OF UROLOGY
(2023)
Article
Oncology
Takero Hirata, Osamu Suzuki, Keisuke Otani, Akimitsu Miyake, Keisuke Tamari, Yuji Seo, Fumiaki Isohashi, Naoki Kai, Koji Hatano, Kazutoshi Fujita, Motohide Uemura, Ryoichi Imamura, Setsuo Tamenaga, Yutaro Yoshino, Yasutoshi Fumimoto, Yasuo Yoshioka, Norio Nonomura, Kazuhiko Ogawa
Summary: This study evaluated the toxicity and efficacy of different SBRT doses for PCa and selected an optimal dose. The results showed that an SBRT dose of 35 Gy per 5 fractions is less likely to cause adverse events than 37.5 and 40 Gy doses in PCa patients.
Review
Oncology
Kazutoshi Fujita, Makoto Matsushita, Marco A. A. De Velasco, Koji Hatano, Takafumi Minami, Norio Nonomura, Hirotsugu Uemura
Summary: The gut microbiome plays a crucial role in the development of diseases like prostate cancer. Gut dysbiosis caused by factors like diet leads to the leakage of gut bacterial metabolites, resulting in prostate cancer growth. Lifestyle factors, especially diet, impact the gut microbiome and are associated with prostate cancer risk. Alterations in the gut microbiome have been identified in patients with prostate cancer, highlighting the importance of considering the gut-prostate axis in screening and treating prostate cancer patients.
Article
Oncology
Nico C. Grossmann, Francesco Soria, Tristan Juvet, Aaron M. Potretzke, Hooman Djaladat, Alireza Ghoreifi, Eiji Kikuchi, Andrea Mari, Zine-Eddine Khene, Kazutoshi Fujita, Jay D. Raman, Alberto Breda, Matteo Fontana, John P. Sfakianos, John L. Pfail, Ekaterina Laukhtina, Pawel Rajwa, Maximillian Pallauf, Cedric Poyet, Giovanni E. Cacciamani, Thomas van Doeveren, Joost L. Boormans, Alessandro Antonelli, Marcus Jamil, Firas Abdollah, Guillaume Ploussard, Axel Heidenreich, Enno Storz, Siamak Daneshmand, Stephen A. Boorjian, Morgan Roupret, Michael Rink, Shahrokh F. Shariat, Benjamin Pradere
Summary: The study compared perioperative and oncologic outcomes among patients who underwent open, laparoscopic, and robotic RNU for non-metastatic upper urinary tract urothelial carcinoma. The results showed worse bladder recurrence-free survival in patients who had laparoscopic and robotic RNU compared to open RNU. However, recurrence-free, cancer-specific, and overall survival were similar between the three surgical approaches. Laparoscopic and robotic RNU were associated with shorter hospital stay and fewer major postoperative complications. Further research is needed to investigate the factors responsible for the inferior bladder recurrence-free survival in patients treated with minimally invasive techniques.
Article
Pathology
Satoshi Nojima, Tokimu Kadoi, Ayana Suzuki, Chiharu Kato, Shoichi Ishida, Kansuke Kido, Kazutoshi Fujita, Yasushi Okuno, Mitsuyoshi Hirokawa, Kei Terayama, Eiichi Morii
Summary: Deep learning systems have been developed to differentiate between follicular thyroid carcinoma and follicular adenoma based on histopathological assessment. The results show that the systems can predict the risk of recurrence, vascular invasion, and wide capsular invasion with a certain level of accuracy based on random views of tumor tissue histology.
Article
Urology & Nephrology
Takafumi Minami, Kazutoshi Fujita, Mamoru Hashimoto, Mitsuhisa Nishimoto, Shogo Adomi, Eri Banno, Masahiro Nozawa, Kazuhiro Nose, Kazuhiro Yoshimura, Masahiro Inada, Masaki Yokokawa, Kiyoshi Nakamatsu, Hirotsugu Uemura
Summary: Patients with prostate cancer treated with brachytherapy may have an increased risk of developing bladder cancer, especially those who smoke heavily and receive external beam radiotherapy as part of their treatment.
WORLD JOURNAL OF UROLOGY
(2023)
Review
Andrology
Makoto Matsushita, Kazutoshi Fujita, Koji Hatano, Marco A. De Velasco, Akira Tsujimura, Hirotsugu Uemura, Norio Nonomura
Summary: The gut microbiota, influenced by both environmental and genetic factors, has been found to have a significant impact on human health. Research has shown a close association between the gut microbiome and various non-intestinal diseases, particularly in the field of cancer biology and therapy. Prostate cancer cells are directly affected by the microbiota in local tissues and urine, suggesting a relationship between prostate cancer cells and the gut microbiota. The bacterial composition of the gut microbiota differs depending on prostate cancer characteristics, such as histological grade and castration resistance. Additionally, certain intestinal bacteria have been shown to be involved in testosterone metabolism, indicating a potential influence on prostate cancer progression and treatment. Basic research suggests that the gut microbiome plays a crucial role in prostate cancer biology through microbial-derived metabolites and components. This review focuses on the emerging relationship between the gut microbiome and prostate cancer, referred to as the gut-prostate axis.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Urology & Nephrology
Mamoru Hashimoto, Nobutaka Shimizu, Saizo Fujimoto, Ken Kuwahara, Mitsuhisa Nishimoto, Shogo Adomi, Eri Banno, Takafumi Minami, Kazutoshi Fujita, Kazuhiro Yoshimura, Akihide Hirayama, Hirotsugu Uemura
Summary: This study aimed to elucidate the pathophysiology of post-micturition dribble (PMD) by analyzing pressure flow study (PFS) findings and symptoms questionnaire. The results showed that PMD was significantly associated with larger prostate volume (PV) and higher bladder outlet obstruction index (BOOI) evaluated by PFS. Furthermore, PMD was found to significantly exacerbate the quality of life (QOL). The severity of PMD and other m-IPSS sub-scores (except nocturia) could have intercorrelation with each other.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2023)
Article
Urology & Nephrology
Naoki Matsumura, Kazutoshi Fujita, Mitsuhisa Nishimoto, Takafumi Minami, Hideo Tahara, Kazuhiro Yoshimura, Hirotsugu Uemura
Summary: The therapeutic landscape for metastatic hormone-sensitive prostate cancer has changed dramatically, with upfront systemic therapy showing improved survival and quality of life. Future research directions include triplet therapy, local therapy, and personalized treatment.
WORLD JOURNAL OF UROLOGY
(2023)
