Article
Plant Sciences
Yaning Cui, Yanxia Zhao, Yuqing Lu, Xiao Su, Yingying Chen, Yingbai Shen, Jinxing Lin, Xiaojuan Li
Summary: The study revealed the factors that regulate the dynamics of aquaporins during water translocation, emphasizing the importance of the cytoskeleton in guard cells and subsidiary cells. This sheds light on the heterogeneous mechanisms that regulate membrane protein dynamics in plants in response to pathogens.
Article
Multidisciplinary Sciences
Sruti Rayaprolu, Sara Bitarafan, Juliet Santiago, Ranjita Betarbet, Sydney Sunna, Lihong Cheng, Hailian Xiao, Ruth S. Nelson, Prateek Kumar, Pritha Bagchi, Duc M. Duong, Annie M. Goettemoeller, Viktor Janos Olah, Matt Rowan, Allan Levey, Levi B. Wood, Nicholas T. Seyfried, Srikant Rangaraju
Summary: By using cell type-specific protein labeling, we can better study the proteomic differences between neurons and astrocytes, and identify potential factors related to neurological diseases.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
S. Andrew Shuster, Jiefu Li, URee Chon, Miley C. Sinantha-Hu, David J. Luginbuhl, Namrata D. Udeshi, Dominique Kiki Carey, Yukari H. Takeo, Qijing Xie, Chuanyun Xu, D. R. Mani, Shuo Han, Alice Y. Ting, Steven A. Carr, Liqun Luo
Summary: A new method called iPEEL was developed to identify cell-surface proteomes in specific cell types in their native tissues. By applying iPEEL to studying developing and mature cerebellar Purkinje cells, researchers discovered differential enrichment in cell-surface proteomes with post-translational protein processing and synaptic functions in these cells. They also identified the critical role of Armh4 in Purkinje cell dendrite morphogenesis through in vivo loss-of-function screening. Disrupting Armh4's endocytosis augmented its effect on dendrite morphogenesis. This study highlights the importance of CSP profiling in native mammalian tissues for understanding cell-surface signaling regulations.
Article
Neurosciences
Priyadharshini Prabhakar, Rainer Pielot, Peter Landgraf, Josef Wissing, Anne Bayrhammer, Marco van Ham, Eckart D. Gundelfinger, Lothar Jaensch, Daniela C. Dieterich, Anke Mueller
Summary: Astrocytes display regional heterogeneity in morphology, function, and molecular composition, and play a role in supporting and modulating neuronal function and signaling. Using mass spectrometry and gene enrichment analysis, we discovered subtle differences in astrocytic proteomes among different brain regions, with the cerebellum showing a strong association with the calcium signaling pathway or bipolar disorder. Additionally, our toolbox proved to be applicable for studying astrocytic proteome differences in vivo.
Article
Multidisciplinary Sciences
Ricardo F. H. Giehl, Paulina Flis, Jorg Fuchs, Yiqun Gao, David E. Salt, Nicolaus von Wiren
Summary: In this study, a method combining fluorescence-activated cell sorting (FACS) with inductively coupled plasma mass spectrometry (ICP-MS) was developed to assess the ionome of different cell populations within Arabidopsis thaliana roots. The results showed that most elements exhibited a radial concentration gradient increasing from the rhizodermis to inner cell layers, and previously unknown ionomic changes were detected due to perturbed xylem loading processes. Additionally, a strong accumulation of manganese in trichoblasts of iron-deficient roots was identified. These findings indicate the existence of cell type-specific constraints for efficient metal sequestration in roots.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Samreen Fathima, Swati Sinha, Sainitin Donakonda
Summary: The study examined glioblastoma at the cell type level in the brain, identifying principal transcription factors and protein regulators that could be targeted for more efficient glioma therapy.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Multidisciplinary Sciences
Xianfu Yi, Zhanye Zheng, Hang Xu, Yao Zhou, Dandan Huang, Jianhua Wang, Xiangling Feng, Ke Zhao, Xutong Fan, Shijie Zhang, Xiaobao Dong, Zhao Wang, Yujun Shen, Hui Cheng, Lei Shi, Mulin Jun Li
Summary: This study revealed the precise modulation of gene expression by context-specific activities of transcription regulators in the nucleus through large-scale data analysis. Exploring across different tissues and cell types, it discovered both common and specific patterns of cooperation among transcription regulators, providing new insights into cell type-specific gene regulation.
Article
Cell Biology
Changfu Yao, Stephanie A. Bora, Tanyalak Parimon, Tanzira Zaman, Oren A. Friedman, Joseph A. Palatinus, Nirmala S. Surapaneni, Yuri P. Matusov, Giuliana Cerro Chiang, Alexander G. Kassar, Nayan Patel, Chelsi E. R. Green, Adam W. Aziz, Harshpreet Suri, Jo Suda, Andres A. Lopez, Gislaine A. Martins, Barry R. Stripp, Sina A. Gharib, Helen S. Goodridge, Peter Chen
Summary: Recent studies have shown immunologic dysfunction in severely ill patients with COVID-19, particularly in those with ARDS, where defective antigen presentation and interferon responsiveness were observed in monocytes. There was also suppressed cytotoxic activity in NK cells and CD8 T lymphocytes, along with deficient B cell activation, indicating delayed viral clearance in severely ill patients. These findings suggest an immune imbalance in COVID-19 patients with ARDS, potentially contributing to a more severe disease course.
Article
Biochemical Research Methods
Natalia Prudente de Mello, Caroline Fecher, Adrian Marti Pastor, Fabiana Perocchi, Thomas Misgeld
Summary: This protocol enables the ex vivo immunocapture of cell-type-specific mitochondria directly from their tissue context in heterogeneous tissues, such as the nervous system, using MitoTag reporter mice. The protocol allows for the selective isolation of functional mitochondria from medium-to-low-abundant cell types, revealing cell-type-specific mitochondrial diversity in molecular composition and function. The approach facilitates the identification of marker proteins to label cell-type-specific organelle populations and elucidates cell-type-enriched mitochondrial metabolic and signaling pathways.
Article
Multidisciplinary Sciences
Rie Kamiyama, Kota Banzai, Peiwei Liu, Abhijit Marar, Ryo Tamura, Fangchao Jiang, Miyuki A. Fitch, Jin Xie, Daichi Kamiyama
Summary: The Drosophila experimental system significantly impacts modern biology studies, with the ability to label endogenously expressed proteins being crucial. Researchers have successfully developed a method using self-complementing split fluorescent proteins in Drosophila to label endogenous proteins in specific cell types and enhance fluorescence signals for subcellular live-cell imaging.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Johanna Simon, Michael Fichter, Gabor Kuhn, Maximilian Brueckner, Cinja Kappel, Jenny Schunke, Tanja Klaus, Stephan Grabbe, Katharina Landfester, Volker Mailaender
Summary: The major challenge in nanocarrier-based anti-cancer vaccination approaches is the targeted delivery of antigens and immunostimulatory agents to specific cells, such as dendritic cells, to induce strong anti-tumor responses. This study presents a surface functionalization strategy to attach antibodies onto nanocarriers, enabling specific targeting and avoiding non-specific uptake. The development of antibody-functionalized nanocarriers for dendritic cell-based vaccination in cancer immunotherapy is greatly facilitated by this novel conjugation technique.
Article
Cell Biology
Salvador Dura-Bernal, Samuel A. Neymotin, Benjamin A. Suter, Joshua Dacre, Joao V. S. Moreira, Eugenio Urdapilleta, Julia Schiemann, Ian Duguid, Gordon M. G. Shepherd, William W. Lytton
Summary: Understanding cortical function requires studying multiple scales, and a multiscale mouse primary motor cortex model with over 10,000 neurons and 30 million synapses has been developed. The model accurately predicts in vivo layer- and cell-type-specific responses associated with behavioral states and experimental manipulations. This theoretical framework sheds light on the cell-type-specific multiscale dynamics associated with several experimental conditions and behaviors.
Article
Engineering, Biomedical
Yih Rue Ong, Robert De Rose, Angus P. R. Johnston
Summary: Nanoparticles show promise for drug delivery, but their rapid clearance by immune cells in blood poses a challenge. In vivo studies reveal a significant role of neutrophils in nanoparticle binding, contrary to ex vivo findings. The increase in circulating neutrophils and decrease in monocytes after intravenous injection affect the overall association of nanoparticles with immune cells.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Plant Sciences
Danilo de Menezes Daloso, Eva Gomes Morais, Karen Fernanda Oliveira e Silva, Thomas Christopher Rhys Williams
Summary: Every plant organ contains different cell types with specialized functions. Investigating cell-type-specific metabolism is challenging due to the complexity of different cells within tissues. However, techniques like cell isolation, microscopy, and modeling have provided insight into cell-type-specific metabolism.
Article
Biochemistry & Molecular Biology
Wei Wei, Nicholas M. Riley, Andrew C. Yang, Joon T. Kim, Stephanie M. Terrell, Veronica L. Li, Marta Garcia-Contreras, Carolyn R. Bertozzi, Jonathan Z. Long
Summary: This study introduced a proximity biotinylation strategy to label, detect, and enrich secreted polypeptides in a cell type-selective manner in mice, resulting in a proteomic atlas of secretomes from various cell types. The research confirmed known cell type-protein pairs, identified new secreted polypeptides distinguishing between cell types, and uncovered a dynamic nutrient-dependent reprogramming of hepatocyte secretome. The strategy enables dynamic and cell type-specific dissection of the plasma proteome and intercellular signaling mediators.
NATURE CHEMICAL BIOLOGY
(2021)