Journal
MOLECULAR MEDICINE REPORTS
Volume 19, Issue 2, Pages 1159-1167Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2018.9722
Keywords
sphingomyelin synthase 1; transforming growth factor 1; epithelial-to-mesenchymal transition; MDA-MB-231 cells
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Funding
- National Natural Science Foundation of China [81560151]
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Breast cancer is the most common cancer in women and a leading cause of cancer-associated mortalities in the world. Epithelial-to-mesenchymal transition (EMT) serves an important role in the process of metastasis and invasive ability in cancer cells, and transforming growth factor 1 (TGF-1) have been investigated for promoting EMT. However, in the present study, the role of the sphingomyelin synthase 1 (SMS1) in TGF-1-induced EMT development was investigated. Firstly, bioinformatics analysis demonstrated that the overexpression of SMS1 negatively regulated the TGF receptor I (TRI) level of expression. Subsequently, the expression of SMS1 was decreased, whereas, SMS2 had no significant difference when MDA-MB-231 cells were treated by TGF-1 for 72 h. Furthermore, the present study constructed an overexpression cells model of SMS1 and these cells were treated by TGF-1. These results demonstrated that overexpression of SMS1 inhibited TGF-1-induced EMT and the migration and invasion of MDA-MB-231 cells, increasing the expression of E-cadherin while decreasing the expression of vimentin. Furthermore, the present study further confirmed that SMS1 overexpression could decrease TRI expression levels and blocked smad family member 2 phosphorylation. Overall, the present results suggested that SMS1 could inhibit EMT and the migration and invasion of MDA-MB-231 cells via TGF-/Smad signaling pathway.
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