Journal
ACS CHEMICAL BIOLOGY
Volume 11, Issue 11, Pages 3165-3171Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.6b00714
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Funding
- Academy of Sciences of the Czech Republic [RVO: 61388963]
- Czech Science Foundation [14-04289S]
- Marie Sklodowska-Curie Innovative Training Network (ITN) Click Gene [H2020-MSCA-ITN-2014-642023]
- Gilead Sciences, Inc. (Foster City, CA, U.S.A.)
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A complete series of 5-substituted uracil or cytosine, as well as 7-substituted 7-deazaadenine and 7-deazaguanine 2'-deoxyribonucleoside triphosphates (dNTPs) bearing substituents of increasing bulkiness (H, Me, vinyl, ethynyl, and phenyl) were systematically studied in competitive primer extension in the presence of their natural counterparts (nonmodified dNTPs), and their kinetic data were determined. The results show that modified dNTPs bearing, pi-electron containing substituents (vinyl, ethynyl, Ph) are typically excellent substrates for DNA polymerases comparable to or better than natural dNTPs. The kinetic studies revealed that these modified dNTPs have higher affinity to the active site of the enzyme primer template complex, and the calculations (semiempirical quantum mechanical scoring function) suggest that it is due to the cation-pi interaction of the modified dNTP with Arg629 in the active site of Bst DNA polymerase.
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