Article
Chemistry, Applied
Renxiang Liu, Xiao-Cong Zhou, Xin-Yi He, Yuan-Qiang Li, Weiqin Zheng, Xiu Wang, Junkai Guo, Chuanfa Ni, Jinbo Hu
Summary: This article presents a general and practical method for the preparation of the new fluorination reagent, SulfoxFluor, and its adaption to large-scale production. The method simplifies the process and reduces safety risks by using chloramine-T trihydrate instead of anhydrous chloramine-T. Furthermore, the research provides valuable insights into the imidation reaction of sulfinyl chlorides with chloramine-T in the presence of water.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Article
Infectious Diseases
Jose Diego Brito-Sousa, Felipe Murta, Sheila Vitor-Silva, Vanderson S. Sampaio, Maxwell O. Mendes, Marcelo A. M. Brito, Talita S. B. Batista, Alicia P. C. Santos, Leonardo L. G. Marques, Laila R. A. Barbosa, Marly M. Melo, Djane C. Baia-da-Silva, Alexandre V. Silva-Neto, Thalie C. Santos, Brenda K. A. Souza, Erick F. G. Figueiredo, Emanuelle L. Silva, Sheila Rodovalho, Theresa H. Nakagawa, Ana Ruth Arcanjo, Andre M. Siqueira, Gisely C. Melo, Judith Recht, Gonzalo J. Domingo, Quique Bassat, Germana Bancone, Wuelton M. Monteiro, Marcus V. G. Lacerda
Summary: This study conducted G6PD screening in malaria treatment units in an endemic area using the CareStart G6PD test, showing high sensitivity but concerning low specificity. Healthcare workers were able to perform the test after training, but limitations in specificity were observed, leading to missed opportunities for radical cure.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Chemistry, Multidisciplinary
Jordi C. J. Hintzen, Yan Luo, Miriam R. B. Porzberg, Paul B. White, Jie Jian, Giordano Proietti, Jasmin Mecinovic
Summary: Histone lysine methylation and acetylation play important roles in regulating gene expression in humans. This study introduces the use of gamma-difluorolysine as a mimic for lysine and F-19 NMR probe to examine histone methylation and acetylation.
CHEMICAL COMMUNICATIONS
(2021)
Article
Microbiology
Alexander B. J. Grey, Melissa M. Cadelis, Yiwei Diao, Duckchul Park, Thomas Lumley, Bevan S. Weir, Brent R. Copp, Siouxsie Wiles
Summary: The study outlines a method to screen fungi for activity against Mycobacteria, revealing that most tested fungi exhibit anti-mycobacterial activity. The research also indicates that growth media may influence antibacterial activity, and that some activity may stem from linoleic acid and other unknown compounds. Ongoing investigations aim to identify the sources of anti-mycobacterial activity and assess the potential for novel bioactive compounds.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Runqing Yang, Dong Wei, Georg Pohnert
Summary: This study reveals that a combination of tryptone and urea as organic nitrogen sources can enhance fucoxanthin production in mixotrophic cultivation of Phaeodactylum tricornutum. Among the amino acids present in the medium, arginine has the highest nitrogen utilization rate and promotes cell growth the most. The optimal combination of arginine and urea as nitrogen sources leads to the highest biomass concentration, fucoxanthin content, and productivity. This study provides new insights into the promotion of fucoxanthin biosynthesis and suggests a promising strategy for efficient enhancement of fucoxanthin production.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Multidisciplinary Sciences
Timothy J. Fazekas, Jill W. Alty, Eliza K. Neidhart, Austin S. Miller, Frank A. Leibfarth, Erik J. Alexanian
Summary: This study reports an approach to achieve diverse functionality on hydrocarbons by utilizing radical chain transfer. By using an easily prepared reagent, a range of functionalizations on small molecules and polyolefins can be achieved. This method has also been applied in the processing of plastic waste and enables the introduction of ionic functionality on polyolefins, transforming them into elastomers with high-value properties.
Article
Chemistry, Multidisciplinary
Samuel Lauzon, Thierry Ollevier
Summary: By designing a novel chiral ligand, high levels of stereoselectivity can be achieved in synthetic reactions. The unique properties of the new ligand can enhance both reaction yields and enantioselectivities.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Wenjing Zhao, Shanjian Liu, Mengqian Yin, Zhisen He, Dongmei Bi
Summary: The use of lignocellulose and nitrogen carriers to produce nitrogen-rich bio-oil and biochar has gained attention for its high-value conversion and utilization of biomass. This study focused on the co-pyrolysis of cellulose with urea and chitosan to produce nitrogen-containing chemicals. The optimal conditions for nitrogen-rich cellulose pyrolysis were analyzed and summarized, revealing the different nitrogen compounds formed with urea and chitosan. The addition of chitosan promoted biochar yield, while the nitrogen content varied with temperature due to the presence of amino groups.
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
(2023)
Article
Geochemistry & Geophysics
Dingwei Gan, Yeting Guo, Xiangmin Lei, Min Zhang, Songsen Fu, Jianxi Ying, Yufen Zhao
Summary: The emergence of prebiotic peptides on the early Earth was a crucial step for the origin of life. Carbamoyl amino acids obtained with nearly 100% yield under prebiotic conditions were reported in this study. The reaction mechanism involves nucleophilic substitution with the release of NH3 from urea, which was verified by 15N-labeling approach. Meanwhile, experiments indicated that carbamoyl amino acids can be also well-obtained under UV radiation in the urea pool, which provides a better condition for carbamoyl amino acids to form prebiotic peptides. The study implies the presence of peptide precursors other than classical amino acids on the primitive Earth.
EARTH AND PLANETARY SCIENCE LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Timo Broese, Peter Ehlers, Peter Langer, Jan von Langermann
Summary: This study investigates the chemoenzymatic preparation of chiral alcohols with a heteroaromatic ring and increasing degrees of fluorination at the alpha-position. The method shows excellent selectivity and yield, with enantiomeric excess ranging from 95 to >99%.
Article
Chemistry, Physical
Jian Zhang, Mingyang Song, Weijun Tang, Dong Xue, Jianliang Xiao, Huaming Sun, Chao Wang
Summary: Converting racemic compounds to enantioenriched products is a common method in accessing chiral molecules. In this study, a kinetic resolution method is used to transform racemic allylic alcohols into chiral compounds, with good yields and stereoselectivities.
Article
Soil Science
Kazumichi Fujii, Chie Hayakawa
Summary: In nitrogen-limited permafrost areas, black spruce roots have the potential to absorb urea, in addition to amino acids and inorganic nitrogen, which is significant for nitrogen acquisition strategies of the trees.
SOIL BIOLOGY & BIOCHEMISTRY
(2022)
Article
Chemistry, Organic
Jitendra L. Nalawade, Pravin C. Mhaske, Abhijit D. Shinde, Abhijit P. Chavan, Yogita K. Abhale, Dhiman Sarkar, Vivek D. Bobade
Summary: A series of novel compounds were efficiently synthesized using molecular simplification, and their activities against Mycobacterium tuberculosis and cytotoxicity on various cell lines were evaluated. The compounds showed promising results in inhibitory activity against the bacteria while being non-toxic to host cells.
Article
Biochemistry & Molecular Biology
Runjin Zhao, Biling Huang, Gang Lu, Songsen Fu, Jianxi Ying, Yufen Zhao
Summary: Amino acid (AA) analysis is crucial in various fields such as biochemistry, food science, and clinical medicine. However, due to limitations, AAs often require derivatization for better separation and determination. In this study, a liquid chromatography-mass spectrometry (LC-MS) method utilizing urea as a derivatization agent was introduced. The urea-derivatized products of 20 AAs exhibited enhanced separation and detection compared to the underivatized counterparts. This fast, simple, and cost-effective method showed potential for AA analysis in complex samples, as demonstrated using a cell culture media model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Organic
Vladimir Kubyshkin, Rebecca Davis, Nediljko Budisa
Summary: Fluorinated proline replacements, such as (4R)- and (4S)-fluoroproline, have shown advantages in elevating protein expression speed and yields, as well as improving folding profiles. They are considered useful tools in biotechnological applications and may potentially be used for proteome-wide proline-to-fluoroproline substitutions in the future. Furthermore, laboratory evolutionary methods with fluoroprolines could enable the introduction of fluorine into living cells, potentially creating synthetic cells with artificial biodiversity containing fluorine as a bioelement.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Eva Havrankova, Vladimir Garaj, Sarka Mascaretti, Andrea Angeli, Zuzana Soldanova, Miroslav Kemka, Jozef Motycka, Marie Brazdova, Jozef Csoellei, Josef Jampilek, Claudiu T. Supuran
Summary: A series of potential human carbonic anhydrase inhibitors were synthesized and evaluated for their inhibition of different isoenzymes. Some compounds showed significant effects on hCA IX isoenzyme and demonstrated good activity against vancomycin-resistant Enterococcus faecalis (VRE).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Jana Curillova, Maria Pechacova, Tereza Padrtova, Daniel Pecher, Sarka Mascaretti, Josef Jampilek, Ludmila Paskova, Frantisek Bilka, Gustav Kovac, Ivan Malik
Summary: This research focused on the synthesis, analysis, physicochemical evaluation, and biological evaluation of hybrid molecules with specific structures. The molecules showed inhibitory effects on mycobacteria and were also tested for toxicity on human liver cancer cells. However, some molecules also exhibited toxicity on normal cells.
APPLIED SCIENCES-BASEL
(2022)
Review
Biochemistry & Molecular Biology
Josef Jampilek, Katarina Kralova
Summary: Climate change and environmental contamination caused by human activities have a growing negative impact on human life. Developing technologies and sophisticated materials, such as graphene-based nanomaterials, could help overcome these negative effects. Graphene-based nanomaterials have promising applications in various fields including medicine and agriculture, but their potential negative effects on living organisms should be carefully considered.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Physical
Josef Jampilek, Katarina Kralova
Summary: Due to the prevalence of drug-resistant microorganisms, there is a decreasing number of new antimicrobial drugs capable of combating these strains. Therefore, formulation innovations and nanotechnologies offer promising alternatives to improve the effectiveness of existing antimicrobial drugs.
Article
Chemistry, Physical
Izabela Jendrzejewska, Tadeusz Gron, Joachim Kusz, Zbigniew Stoklosa, Ewa Pietrasik, Tomasz Goryczka, Bogdan Sawicki, Jerzy Goraus, Josef Jampilek, Henryk Duda, Beata Witkowska-Kita
Summary: This study investigates the magnetic, electrical, and thermal properties of ZnCr2S4:Pb single crystals, revealing magnetic interactions and spin fluctuations induced by the incorporation of lead ions.
Article
Chemistry, Medicinal
Tomas Gonec, Dominika Pindjakova, Lucia Vrablova, Tomas Strharsky, Hana Michnova, Tereza Kauerova, Peter Kollar, Michal Oravec, Izabela Jendrzejewska, Alois Cizek, Josef Jampilek
Summary: The prepared carbamate compounds showed high activity against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA), with most compounds having a high selectivity index. However, these compounds were ineffective against Enterococcus faecalis.
Article
Biochemistry & Molecular Biology
Dawid Wardecki, Malgorzata Dolowy, Katarzyna Bober-Majnusz, Josef Jampilek
Summary: This study aimed to evaluate the lipophilicity of a series of substances lowering the concentration of uric acid in blood and anti-androgen drugs. Thin-layer chromatography in reversed-phase systems and computational methods were used. The results showed that the experimental and calculated logP values gave similar results, and the RP-TLC or RP-HPTLC systems can be successfully applied to estimate the lipophilicity of the studied compounds. Chemometric methods were also used to compare the compounds and a new approach based on the sum of ranking differences was applied to compare the chromatographically obtained and theoretical lipophilicity descriptors.
Editorial Material
Biochemistry & Molecular Biology
Milos Hricovini, Josef Jampilek
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Izabela Jendrzejewska, Tomasz Goryczka, Ewa Pietrasik, Joanna Klimontko, Josef Jampilek
Summary: Twelve drugs containing sildenafil compounds were analyzed using X-ray studies and thermal analysis. The presence of sildenafil was confirmed in all investigated drugs, and the diffraction patterns and interplanar distances were consistent with the ICDD database. The combination of X-ray study and thermal analysis can help identify irregularities in pharmaceutical specimens, such as counterfeit products or inaccurate amounts of active substance.
Review
Chemistry, Physical
Dagmara Slota, Karina Pietak, Josef Jampilek, Agnieszka Sobczak-Kupiec
Summary: The conventional intake of drugs and active substances through oral administration often results in unwanted side effects and the need for increased dosage to achieve the desired effect, as the substances also distribute to other tissues. Tissue engineering offers a solution by designing intelligent systems to deliver active substances precisely to the site of disease conversion.
Article
Chemistry, Multidisciplinary
Dagmara Slota, Karina Pietak, Wioletta Florkiewicz, Josef Jampilek, Agnieszka Tomala, Mateusz M. M. Urbaniak, Agata Tomaszewska, Karolina Rudnicka, Agnieszka Sobczak-Kupiec
Summary: Bioactive calcium phosphate ceramics (CaPs) are important components of bone structure. This study compared different ceramic powders and analyzed their physicochemical properties. The results confirmed the purity of the materials and the validity of the synthesis methods. Additionally, the specific surface area of the ceramics was found to affect drug release efficiency.
Article
Chemistry, Multidisciplinary
Ewa Kisiel-Nawrot, Malgorzata Latocha, Andrzej Bak, Violetta Kozik, Josef Jampilek, Andrzej Zieba
Summary: Evaluated the antitumor potency of antibacterial quinobenzothiazines against different types of human cancer cell lines. Identified the most active compounds and investigated the structure-activity relationship. Explored the potential ligand-enzyme compositions and binding mode of the most potent agents.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Ewa Kisiel-Nawrot, Dominika Pindjakova, Malgorzata Latocha, Andrzej Bak, Violetta Kozik, Kinga Suwinska, Alois Cizek, Josef Jampilek, Andrzej Zieba
Summary: This paper presents a new method for synthesizing hybrid combinations of 1,2,3-triazoles with a tetracyclic quinobenzothiazinium system. The synthesized compounds were tested for their anticancer and antibacterial activity, and the results showed a dependence on the substituent type and its position in the quinobenzothiazinium system. Additionally, distance-guided property evaluation was performed using principal component analysis and hierarchical clustering analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Izabela Jendrzejewska, Tadeusz Gron, Joachim Kusz, Zbigniew Stoklosa, Ewa Pietrasik, Tomasz Goryczka, Bogdan Sawicki, Jerzy Goraus, Josef Jampilek, Beata Witkowska-Kita
Summary: This study obtained and investigated rhenium-doped zinc chromium selenide single crystals using chemical vapour transport. X-ray study confirmed the cubic structure of the crystals. Thermal, magnetic, electrical, and specific heat measurements accurately determined the physicochemical characteristics. The results showed that the obtained single crystals are p-type semiconductors with antiferromagnetic order below the Neel temperature T-N = 21.7 K. The substitution of rhenium ions caused an increase in several properties compared to pure zinc chromium selenide, and the crystals were thermally stable up to 1100 degrees C.
Article
Biochemistry & Molecular Biology
Izabela Jendrzejewska, Robert Musiol, Tomasz Goryczka, Ewa Pietrasik, Joanna Klimontko, Josef Jampilek
Summary: X-ray powder diffraction and thermal analysis are solid-state techniques that can be used to identify and quantify chemical compounds. In this study, 31 dietary supplements were analyzed to confirm the presence of iron compounds claimed by the manufacturers, and the influence of excipients on peak shapes and positions was observed.