Article
Parasitology
Yihan Wu, Zhu Ying, Jing Liu, Zhepeng Sun, Shuang Li, Qun Liu
Summary: This study identified and characterized the ANT protein in T. gondii. Depletion of TgANT inhibited parasite growth, impaired mitochondrial and apicoplast biogenesis, and resulted in abnormal cell division.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Justin Sui, Jennifer C. Boatz, Jian Shi, Qianjiang Hu, Xiaoyun Li, Yingze Zhang, Melanie Koenigshoff, Corrine R. Kliment
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease characterized by lung scarring and remodeling. This study investigates the role of adenine nucleotide translocases (ANTs) in IPF pathogenesis and finds that the loss of ANT1 leads to mitochondrial dysfunction, increased cellular senescence, and enhanced lung fibrosis. The findings suggest that modulation of ANTs could be a potential therapeutic avenue for limiting pulmonary fibrosis.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Andrea Doerner, Oleg Lynetskiy, Gerhild Euler, Ulf Landmesser, Klaus-Dieter Schlueter, Jacqueline Heger
Summary: Transgenic overexpression of ANT1 showed stronger protective effects on mitochondrial function than wild-type rats, coping with I/R injury more efficiently by maintaining mitochondrial energy balance, improving diastolic function, and enhancing calcium dynamics after reperfusion.
Article
Biochemical Research Methods
Jean Finlayson, Neusha Barakati, Paul R. Langlais, Janet Funk, Rocio Zapata Bustos, Dawn K. Coletta, Moulun Luo, Wayne T. Willis, Lawrence J. Mandarino
Summary: This study characterized ANT1 abundance and acetylation in human skeletal muscle using a novel method with small amounts of tissue samples. The findings indicate that acetylation of Lys23 reduces ADP binding to ANT1, providing insights into the potential effects of acetylation on ANT1 function in human muscle.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Entomology
Zhen He, Yang Fang, Fengchao Zhang, Yang Liu, Xinkai Cheng, Jiajia Wang, Dechen Li, Dengsong Chen, Fan Wu
Summary: In Drosophila, Ant2 plays a crucial role in spermatid individualization and maturation. Knockdown of Ant2 leads to abnormalities in spermatid individualization and sperm maturation, resulting in male infertility. Ant2 knockdown also causes a decrease in tubulin polyglycylation, disruption of mitochondrial function, and an increase in apoptotic cells. Transcriptome and proteome analyses reveal significant changes in gene and protein expression, including reproductive-related glutathione transferases.
Article
Biochemistry & Molecular Biology
Veronica Demicheli, Florencia Tomasina, Santiago Sastre, Ari Zeida, Veronica Tortora, Analia Lima, Carlos Batthyany, Rafael Radi
Summary: The interaction between cytochrome c and cardiolipin plays a crucial role in mitochondrial redox homeostasis by influencing electron transfer and protein conformation. Peroxynitrite-induced nitration of cytochrome c is enhanced by cardiolipin, targeting the Tyr67 site specifically. This nitration modifies protein-cardiolipin affinity and could impact processes such as apoptosis by altering mitochondrial redox balance.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Pavla Pruchova, Klara Gotvaldova, Katarina Smolkova, Lukas Alan, Blanka Holendova, Jan Tauber, Alexander Galkin, Petr Jezek, Martin Jaburek
Summary: This study reveals the antioxidant and anti-inflammatory roles of iPLA(2)gamma in the brain. It demonstrates that iPLA(2)gamma is activated by tert-butylhydroperoxide and releases free fatty acids, which leads to an increase in respiratory rate and a decrease in mitochondrial superoxide production. It also shows that iPLA(2)gamma plays a key role in the remodeling of cardiolipins in the brain.
Article
Cell Biology
Fatih Yergoz, Julian Friebel, Nicolle Kraenkel, Ursula Rauch-Kroehnert, Heinz-Peter Schultheiss, Ulf Landmesser, Andrea Doerner
Summary: ANT1 in ischemic heart tissue promotes an anti-inflammatory immune response by increasing the infiltration of anti-inflammatory cells and reducing the activity of pro-inflammatory cells, resulting in improved cardiac function.
Article
Cell Biology
Corrine R. Kliment, Jennifer M. K. Nguyen, Mary Jane Kaltreider, YaWen Lu, Steven M. Claypool, Josiah E. Radder, Frank C. Sciurba, Yingze Zhang, Alyssa D. Gregory, Pablo A. Iglesias, Venkataramana K. Sidhaye, Douglas N. Robinson
Summary: Airway hydration and ciliary function are critical to airway homeostasis and dysregulated in chronic obstructive pulmonary disease (COPD), and members of the mitochondrial ADP/ATP transporter family adenine nucleotide translocase (ANT) play a protective role against cigarette smoke, highlighting a potential for upregulation of ANT proteins in COPD protection.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yan Wang, Chun Jie Wu, Yu Du, Yu Qing Liu, Jing Ran Cai, Xue Qing Wu, Shu Qun Hu
Summary: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are produced during renal ischemia/reperfusion, with peroxynitrite being a major player in renal cell apoptosis. Inhibiting the nitration of SIRT2 tyrosine could be a promising strategy to prevent ischemic renal diseases involving AKI, as it leads to impaired renal function and cell apoptosis.
FREE RADICAL RESEARCH
(2021)
Article
Cell Biology
Tatiana A. Tarasenko, Ekaterina S. Klimenko, Vladislav Tarasenko, Milana Koulintchenko, Andre Dietrich, Frederique Weber-Lotfi, Yuri M. Konstantinov
Summary: Mitochondria can import RNA and DNA fragments through distinct channels, which may be activated based on the size of the DNA substrate. Analysis of knockout lines in Arabidopsis thaliana revealed different VDAC isoforms' involvement in alternative channel formation. Several outer and inner membrane proteins are proposed as partners of VDAC in these pathways.
Review
Cell Biology
Elena Frigo, Ludovica Tommasin, Giovanna Lippe, Michela Carraro, Paolo Bernardi
Summary: This article introduces the formation of Ca2+-activated, high-conductance channels by F1FO (F)-ATP synthase and adenine nucleotide translocase (ANT) in the inner membrane of mitochondria from various eukaryotes. The permeability transition (PT), a Ca2+-dependent increase in permeability in the inner mitochondrial membrane, and its molecular mechanisms have been challenging for scientists for the past 70 years. The features of the PT (or lack thereof) in mammals, yeast, fruit flies, brine shrimp, and nematodes, as well as the intrinsic pathway of apoptosis and other forms of cell death, are discussed in this review.
Article
Physiology
Tomas A. Schiffer, Liza Loef, Radiosa Gallini, Masood Kamali-Moghaddam, Mattias Carlstroem, Fredrik Palm
Summary: The study reveals a dynamic interaction between ANT2 and UCP2 that is regulated by mitochondrial respiratory activity, potentially impacting mitochondrial efficiency and cellular substrate utilization.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Xavier R. Chapa-Dubocq, Jorge F. Garcia-Baez, Jason N. Bazil, Sabzali Javadov
Summary: This study investigates the modulation of mitochondrial metabolism and function by changes in matrix Ca2+. It identifies the main mechanism responsible for mitochondrial Ca2+ overload and proposes a potential role of adenine nucleotide translocator (ANT) in the gating of mitochondrial permeability transition pores (PTP).
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Engineering, Biomedical
Jinyan Zhang, Liwei Liu, Zhen Dong, Xicun Lu, Wenxuan Hong, Jin Liu, Xiaoyi Zou, Jinfeng Gao, Hao Jiang, Xiaolei Sun, Kai Hu, Youjun Yang, Junbo Ge, Xiao Luo, Aijun Sun
Summary: This study utilizes a peroxynitrite-triggered carbon monoxide donor to generate a biomimetic carbon monoxide nanogenerator, which is coated with macrophage membrane for targeted delivery to the ischemic area and neutralization of proinflammatory cytokines, effectively improving myocardial ischemia-reperfusion injury. This research provides a new perspective on the safe therapeutic use of carbon monoxide for myocardial ischemia-reperfusion injury by utilizing a novel carbon monoxide donor combined with biomimetic technology. The M/PCOD@PLGA nanogenerator offers targeted delivery of carbon monoxide to the ischemic area, minimizing potential toxicity and enhancing therapeutic efficacy.
BIOACTIVE MATERIALS
(2023)