Article
Physics, Applied
Yundong Liu, Hailiang Chen
Summary: The study proposed a dual-polarized photonic crystal fiber sensor based on SPR for measuring the refractive indexes of liquids in the range of 1.36-1.42, achieving dual-polarized detection through external SPR effect, with maximum RI sensitivities of 15,900 nm RIU-1 and 16,900 nm RIU-1, respectively.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Chemistry, Analytical
Edwin J. Ortiz-Riano, Diana L. Mancera-Zapata, Martha Ulloa-Ramirez, Fernando Arce-Vega, Eden Morales-Narvaez
Summary: Macromolecular association is important in biomedical sciences. This study presents a real-time biosensing platform using graphene oxide-modified microwell plates and fluorophore-labeled proteins to determine protein-binding constants. The platform proves to be comparable and consistent with existing methods in determining protein kinetics constants.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Martina Vrablova, Katerina Smutna, Ivan Koutnik, Dominika Markova, Daniel Vrabl, Kamil Maciej Gorecki, Radim Zebrak
Summary: In order to overcome the limitations of traditional methods for measuring membrane permeability, we propose a new method based on surface plasmon resonance imaging (SPRi) that allows for rapid evaluation of membrane permeability. Through our experiments, we have demonstrated the effectiveness of this method in studying the penetration behavior of organic compounds in plant organs.
Article
Chemistry, Analytical
Pengfei Zhang, Rui Wang, Zijian Wan, Xinyu Zhou, Guangzhong Ma, Jayeeta Kolay, Jiapei Jiang, Shaopeng Wang
Summary: The article introduces a total internal reflection-based evanescent scattering microscopy technique that can detect single proteins without labeling and provides a high-precision measurement method for protein binding kinetics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Pengfei Zhang, Xinyu Zhou, Jiapei Jiang, Jayeeta Kolay, Rui Wang, Guangzhong Ma, Zijian Wan, Shaopeng Wang
Summary: Plasmonic scattering microscopy (PSM) adds a dry objective to a surface plasmon resonance (SPR) system, providing higher spatial resolution and larger field of view. PSM allows studying multiple cells simultaneously and quantifying the mechanical properties of cell-substrate contacts.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Optics
Yong Wei, Chen Shi, Chunlan Liu, Chunbiao Liu, Xingkai Wang, Yixiong Tang, Rui Wang, Zhihai Liu
Summary: In this paper, a 3D displacement sensor based on fiber SPR is proposed to measure micro displacement in X, Y and Z axes.
Article
Optics
Shu-cheng Lo, Chia-wei Lee, Ruey-lin Chern, Pei-kuen Wei
Summary: In this work, high-performance surface plasmonic sensors using gold nanostructures and Bragg photonic structures are presented. The hybrid Tamm-plasmon mode, resulting from the Fano coupling between higher order Tatum plasmon states and Bloch-wave surface plasmon polariton on the gold nanoslit array, demonstrates high surface sensitivity and resonant quality. The experimental results show that the hybrid mode has significantly higher sensitivity and better signal-to-noise ratios in biomolecular interaction measurement.
Article
Chemistry, Multidisciplinary
Chun-Yuan Wang, Yungang Sang, Xinyue Yang, Soniya S. Raja, Chang-Wei Cheng, Haozhi Li, Yufeng Ding, Shuoyan Sun, Hyeyoung Ahn, Chih-Kang Shih, Shangjr Gwo, Jinwei Shi
Summary: This study demonstrates a strong coupling system achieved by coupling localized surface plasmon modes. Under specific conditions, a root N scaling of Rabi splitting energy is observed, along with a confirmed giant Rabi splitting in the visible spectral range. Additionally, the coupling strength reaches the ultrastrong coupling regime in some cases, representing about 10% of the mode energy.
Article
Chemistry, Multidisciplinary
Batakrishna Jana, Seongeon Jin, Eun Min Go, Yumi Cho, Dohyun Kim, Sangpil Kim, Sang Kyu Kwak, Ja-Hyoung Ryu
Summary: Lysosomes are important organelles and targets for cancer therapy. Recent studies have shown that lysosomal membrane permeabilization can induce cell death, providing an effective way to treat cancer. However, most lysosome-targeted anticancer drugs lack selectivity for cancer cells. This study demonstrates the use of intra-lysosomal self-assembly of a peptide amphiphile as a powerful technique to overcome this problem. The peptide amphiphile localizes in the cancer lysosome and undergoes enzyme-instructed supramolecular assembly, leading to lysosomal damage and caspase-independent apoptotic death of cancer cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Zhijian Mai, Ye Yuan, Jung-Shen B. Tai, Bohdan Senyuk, Bing Liu, Hao Li, Yao Wang, Guofu Zhou, Ivan I. Smalyukh
Summary: Dispersing pentagonal gold bipyramids in nematic liquid crystals allows for narrow and controlled polarization-dependent surface plasmon resonance spectra, with easy electric switching. The bipyramids orient with their C-5 rotation symmetry axes along the nematic director, showing spatially homogeneous density in aligned samples. Topological solitons enable spatial reorganization of the nanoparticles based on elastic free energy density within their micrometer-scale structures.
Article
Biochemical Research Methods
Francesco Barbero, Sara Michelini, Oscar H. Moriones, Javier Patarroyo, Jordi Rosell, Muriel F. Gusta, Michele Vitali, Luna Martin, Francesc Canals, Albert Duschl, Jutta Horejs-Hoeck, Laura Mondragon, Neus G. Bastus, Victor Puntes
Summary: Sodium citrate-stabilized gold nanoparticles can be destabilized in cell culture media, leading to their aggregation and formation of a protein corona. The presence of certain substances, such as antibiotics, can alter the formation and composition of the corona, resulting in changes in nanoparticle uptake.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yujia Zhou, Xingtao Zhou, Tao Hong, Wucheng Qi, Ke Zhang, Fang Geng, Shaoping Nie
Summary: Tea polysaccharides (TPs) inhibit the proliferation of colon cancer cells CT26 by targeting lysosomes and inducing apoptosis, mainly through disrupting lysosome function, causing mitochondrial damage, and activating caspase cascade.
Article
Chemistry, Analytical
Teliang Zhang, Xueliang Wang, Youjun Zeng, Songfeng Huang, Xiaoqi Dai, Weifu Kong, Qian Liu, Jiajie Chen, Junle Qu, Yonghong Shao
Summary: Surface plasmon resonance microscopy (SPRM) is widely used in biological fields for its high spatial resolution and label-free detection. In this study, a home-built SPRM system based on total internal reflection (TIR) was utilized to study the imaging of a single nanoparticle. By using a ring filter and deconvolution algorithm, the parabolic tail of the nanoparticle image was removed, achieving a spatial resolution of 248 nm. Additionally, specific binding between human IgG antigen and goat anti-human IgG antibody was successfully measured using TIR-based SPRM, demonstrating its capability in imaging sparse nanoparticles and monitoring biomolecular interactions.
Article
Chemistry, Multidisciplinary
Timothy J. J. Palinski, Bin Guan, Bronwyn H. H. Bradshaw-Hajek, Michael A. A. Lienhard, Craig Priest, Felix A. Miranda
Summary: This paper presents a colorimetric sensing system for rapid detection of gas-phase analytes from flowing micro-volume fluid samples. The sensor platform combines an analyte-responsive metal-insulator-metal (MIM) thin-film structure with a large area quartz micropillar array, allowing precise alignment and separation of optical and fluidic structures. The sensor's response time is reduced from minutes to seconds, making it suitable for portable/wearable devices. The platform shows potential for continuous, compact, and quantitative colorimetric analysis of volatile analytes in low-volume samples.
Article
Chemistry, Analytical
Xinpu Zhang, Zeliu Li, Wen Yan, Ang Li, Fenglin Zhang, Xiaotong Li, Mengdi Lu, Wei Peng
Summary: Prism-based surface plasmon resonance (SPR) system is a leading candidate concept for scale application and commercial solution. By optimizing and integrating the optical system, a miniaturized and customizable SPR system has been developed to meet the various needs of different users and application scenes.
Article
Biochemistry & Molecular Biology
Esperanza Rivera-de-Torre, Juan Palacios-Ortega, Jessica E. Garb, J. Peter Slotte, Jose G. Gavilanes, Alvaro Martinez-del-Pozo
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)
Article
Biochemistry & Molecular Biology
Miriam Olombrada, Cohue Pena, Olga Rodriguez-Galan, Purnima Klingauf-Nerurkar, Daniela Portugal-Calisto, Michaela Oborska-Oplova, Martin Altvater, Jose G. Gavilanes, Alvaro Martinez-del-Pozo, Jesus de la Cruz, Lucia Garcia-Ortega, Vikram Govind Panse
NUCLEIC ACIDS RESEARCH
(2020)
Article
Microbiology
Angelica Partida-Hanon, Moises Maestro-Lopez, Stefania Vitale, David Turra, Antonio Di Pietro, Alvaro Martinez-del-Pozo, Marta Bruix
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Juan Palacios-Ortega, Esperanza Rivera-de-Torre, Jose G. Gavilanes, J. Peter Slotte, Alvaro Martinez-del-Pozo
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2020)
Article
Biochemistry & Molecular Biology
Juan Palacios-Ortega, Esperanza Rivera-de-Torre, Sara Garcia-Linares, Jose G. Gavilanes, Alvaro Martinez-del-Pozo, J. Peter Slotte
Summary: Sticholysins are pore-forming toxins produced by sea anemones, capable of oligomerizing in solution and forming pores with specific stoichiometry. The study also indicates that the stoichiometry of actinoporin pores is conserved in equilibrium, regardless of the particular composition.
Review
Biochemistry & Molecular Biology
Esperanza Rivera-de-Torre, Juan Palacios-Ortega, J. Peter Slotte, Jose G. Gavilanes, Alvaro Martinez-del-Pozo, Sara Garcia-Linares
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Juan Palacios-Ortega, Sara Garcia-Linares, Esperanza Rivera-de-Torre, Diego Heras-Marquez, Jose G. Gavilanes, J. Peter Slotte, Alvaro Martinez-del-Pozo
Summary: Actinoporins are a family of pore-forming toxins produced by sea anemones, which exert their activity by specifically recognizing sphingomyelin at their target membranes and killing cells. This review examines the structural features of actinoporins down to the residue level, focusing on the roles of specific amino acids in their function and fold. The membrane requirements for pore formation, the effect of lipid composition, and the process of pore formation are also discussed in light of the latest findings in the field.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Sara Garcia-Linares, Rafael Amigot-Sanchez, Carmen Garcia-Montoya, Diego Heras-Marquez, Carlos Alfonso, Juan Roman Luque-Ortega, Jose G. Gavilanes, Alvaro Martinez-Del-Pozo, Juan Palacios-Ortega
Summary: Sticholysins are pore-forming toxins produced by the sea anemone Stichodactyla helianthus. StnII can enhance the activity of StnI and promote its oligomerization. Analytical ultracentrifugation experiments show that StnI is more prone to oligomerization in water solution, but a small amount of StnII in StnI-StnII mixtures can promote oligomerization.
Review
Biochemistry & Molecular Biology
Juan Palacios-Ortega, Diego Heras-Marquez, Rafael Amigot-Sanchez, Carmen Garcia-Montoya, Carlos Torrijos, Diego Laxalde, Jose G. Gavilanes, Sara Garcia-Linares, Alvaro Martinez-del-Pozo
Summary: Spanish or Spanish-speaking scientists constitute a significant group studying pore-forming proteins, with a focus on actinoporins. These proteins can transition from a soluble form to a membrane-bound state, triggering cell death by recognizing specific components in the membrane. Our research team from Universidad Complutense of Madrid has been investigating the role of sterols in this transition process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Juan Palacios-Ortega, Rafael Amigot-Sanchez, Carmen Garcia-Montoya, Ana Gorse, Diego Heras-Marquez, Sara Garcia-Linares, Alvaro Martinez-Del-Pozo, J. Peter Slotte
Summary: This study provides evidence for the contact of Sticholysins with phosphatidylcholine lipids and reveals the different effects of cholesterol on each Sticholysin isotoxin in membranes. Additionally, the depth of tryptophan residues of Sticholysins in the bilayer was investigated.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Saurabh Saraswat, Archana Chugh
Summary: Cell penetrating peptides (CPP) are important tools for intracellular delivery of molecules. In this study, a novel marine-derived CPP from Engraulis japonicus, called Engraulisin, was reported. Engraulisin demonstrated successful cellular uptake and selective antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA), making it a potential candidate for drug delivery.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Niu, Si Jia Chen, Jeffery B. Klauda
Summary: Macrophage membranes in the activated state are more tightly packed, exhibit increased chain order across lipid species, and form specific lipid clusters. These findings provide physiologically accurate models for future computational studies of macrophage membranes and their proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Niki Baccile, Vincent Chaleix, Ingo Hoffmann
Summary: This study measured the bending rigidity of self-assembled structures formed by a new biobased glucolipid bioamphiphile using neutron spin-echo (NSE) technology. The results showed that the bending rigidity of these structures is lower or higher compared to phospholipid membranes, providing a new theoretical basis for the application of these new molecular systems.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Alain Bolano Alvares, Pablo E. A. Rodriguez, Gerardo D. Fidelio
Summary: The behavior of amphiphilic molecules at the air/water interface was studied to evaluate the arrangement formed in a confined area. The surface properties of zwitterionic DPPC lipid and A beta(1-40) amyloid peptide in mixed films were investigated at different temperatures. DPPC undergoes a phase transition depending on temperature and lateral pressure, which allows for the study of its influence on amyloid structure formation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Lukasz Plachta, Marzena Mach, Magdalena Kowalska, Pawel Wydro
Summary: Resveratrol, a popular phytoalexin found in grapes and red wine, has beneficial effects on the cardiovascular system, exhibits antiviral, antibacterial, and antifungal properties, and may have therapeutic effects against cancer. This study investigates the interaction of resveratrol with model cell membranes, demonstrating changes in their physicochemical parameters and highlighting the role of cholesterol content in resveratrol incorporation. The findings suggest that the molecular mechanism of action of resveratrol may involve interactions with lipid rafts.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Edurne Rujas, Beatriz Apellaniz, Johana Torralba, David Andreu, Jose M. M. Caaveiro, Shixia Wang, Shan Lu, Jose L. Nieva
Summary: This study investigates the role of fusion peptide and Trp-rich membrane proximal external region in HIV-1 fusion and demonstrates that liposome-based formulations containing FP-MPER hybrid peptides can induce the production of specific neutralizing antibodies in rabbits. The results support the use of liposomes as vaccine carriers and the inclusion of lipid membranes in immunogens to elicit specific humoral responses.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Dailin Li, Dingyuan Shi, Lei Wang
Summary: G protein-gated inwardly rectifying potassium (GIRK) channels play a significant role in regulating cell excitability. This study investigated the ion permeation mechanism in GIRK2 mutants using molecular dynamic simulations and QM/MM methods. The results revealed the importance of a multi-ion distribution for ion conduction.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Max J. den Uijl, Arnold J. M. Driessen
Summary: Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. The phospholipid dependence of membrane protein insertion has remained mostly unknown. This study reveals that the insertion of the mannitol permease MtlA into the membrane depends on the presence of phosphatidylglycerol and is stimulated by phosphatidylethanolamine.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick Allen, Adam C. Smith, Vernon Benedicto, Abbas Abdulhasan, Vasanthy Narayanaswami, Enrico Tapavicza
Summary: We explore the stability, size, and structure of nanodiscs formed by the N-terminal domain of apolipoprotein E3 and a variable number of 1,2-dimyristoyl-sn-glycero-3-phosphocholine molecules. Our simulations suggest that nanodiscs containing 240 to 420 DMPC molecules are stable. The antiparallel configuration shows more protein-protein interactions and ionic contacts, as well as greater stability and rigidity compared to the parallel configuration.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick M. Keating, Nicholas P. Schifano, Xinrui Wei, Matthew Y. Kong, Jinwoo Lee
Summary: Lassa virus (LASV) infection is dependent on the fusion of its viral membrane with the host cell membrane, and our study reveals that this fusion process involves pH-dependent conformational changes in the transmembrane domain of LASV. These findings provide valuable insights into the fusion mechanism of LASV and can be utilized in the design of therapeutics to combat Lassa virus infections and prevent its potential spread.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Ashley L. Bennett, Kristen N. Cranford, Austin L. Bates, Christopher R. Sabatini, Hee-Seung Lee
Summary: This study employed molecular dynamics simulations to elucidate the binding, folding, and insertion details of TP10W in the cell membrane. The simulations revealed that TP10W can translocate across the lipid membrane as a monomer, and the charged amino acid side chains can move between lipid leaflets. Comparison with point mutated variants demonstrated significant conformational changes due to charge distribution.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Jessica H. van Wonderen, Jason C. Crack, Marcus J. Edwards, Thomas A. Clarke, Gerhard Saalbach, Carlo Martins, Julea N. Butt
Summary: Electrogenic bacteria transfer excess respiratory electrons to metal oxide particles and electrodes through the MtrCAB complex. The crystal structure of MtrCAB from S. baltica OS185 was resolved, and liquid chromatography mass spectrometry was used to determine the mass values of the three proteins in purified MtrCAB complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Review
Biochemistry & Molecular Biology
Yi-Ming Chen, Ching-Tai Lu, Chia-Wen Wang, Wolfgang B. Fischer
Summary: A series of dye ligands were ranked based on docking experiments, selecting the most suitable ligands for binding with different proteins. Additionally, a comparison study was conducted on a series of repurposing drugs and known antivirals.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
