Journal
MICROCHIMICA ACTA
Volume 186, Issue 2, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3236-9
Keywords
Staphylococcus aureus; Dendritic nucleic acid nanostructure; Hybridization chain reaction; Electrochemical nanoprobe; ELISA; Contaminated food; Toxic protein; Ruthenium(III) hexammine; Electrostatic adsorption
Categories
Funding
- National Key Research and Development Program of China [2018YFC1602800]
- National Natural Science Foundation of China [21804071]
- Natural Science Foundation of Jiangsu Province of China [BK20180688]
- University Science Research Project of Jiangsu Province in China [16KJB550005]
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A rapid and ultrasensitive method is described for the detection of staphylococcal enterotoxin B (SEB). It is based on the formation of a dendritic DNA superstructure by integrating (a) target-induced triggering of DNA release with (b) signal amplification by a hybridization chain reaction. Partially complementary pairing of aptamer and trigger DNA forms a duplex structure. The capture DNA is then placed on the surface of a gold electrode through gold-thiol chemistry. In the presence of SEB, the aptamer-target conjugate is compelled to form. This causes the release of trigger DNA owing to a strong competition with SEB. The trigger DNA is subsequently hybridized with the partial complementary sequences of the capture DNA to trigger HCR with three auxiliary DNA sequances (referred to as H1, H2, H3). Finally, the dendritic DNA superstructure is bound to hexaammineruthenium(III) cation by electrostatic adsorption and assembled onto the modified gold electrode. This produces an amplified electrochemical signal that is measured by chronocoulometry. Under optimal conditions, the charge difference increases linearly with the logarithm of the SEB concentrations in the range from 5pgmL(-1) to 100ngmL(-1) with a detection limit as low as 3pgmL(-1) (at S/N=3).
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