Journal
MICROCHIMICA ACTA
Volume 186, Issue 2, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-018-3193-8
Keywords
Mercury ion; T-Hg2+-T; Exonuclease III; AuNPs; Visual detection; UV-vis absorption
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Funding
- National Natural Science Foundation of China [31471644]
- Primary Research AMP
- Development Plan of Shandong Province [2017GSF220009]
- University of Jinan Scientific Research Fund [XKY1421]
- Doctoral Fundation of University of Jinan [XBS1431]
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A colorimetric biosensor and visual test is described for the determination of mercury(II). It relies on the specific thymine-Hg(II)-thymine (T-Hg2+-T) interaction which induces a cyclic amplification process (caused by the enzyme exonuclease III) and the aggregation of gold nanoparticles. These results in a color change from red to violet. Under optimized conditions, this colorimetric assay (best performed at 524 nm) has a detection limit as low as 0.9 nM with a detection range over 4 orders of magnitude (from 1 nM to 10 mu M).
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