Review
Biotechnology & Applied Microbiology
Kai Ma, Chun-Liu Mi, Xiang-Xiang Cao, Tian-Yun Wang
Summary: Gene delivery systems are crucial for gene therapy and recombinant protein production, with non-viral vector gene delivery reagents offering advantages such as large packaging load capacity and low immunogenicity. Liposomes and non-liposome cationic polymers are commonly used as gene delivery reagents, with different head structures including quaternary ammonium salt, amine, amino acid, polypeptide, guanidine salt, and a heterocyclic ring.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anna A. Efimova, Anton S. Popov, Alexey V. Kazantsev, Pavel I. Semenyuk, Irina M. Le-Deygen, Nikolay V. Lukashev, Alexander A. Yaroslavov
Summary: pH-sensitive liposomes with embedded AMS showed rapid cargo release when pH was altered, but the mechanism is not yet fully understood. This article presents the details of fast cargo release based on ATR-FTIR spectroscopy and molecular modeling. The findings are relevant to the potential use of AMS-containing pH-sensitive liposomes for drug delivery.
Article
Pharmacology & Pharmacy
Yunchun Zhao, Haili Zheng, Xiaorong Wang, Xiaoling Zheng, Yongquan Zheng, Yue Chen, Weidong Fei, Jiahuan Zhu, Wenxi Wang, Caihong Zheng
Summary: Novel cationic lipid-based liposomes prepared using a new lipid material have shown promising results in enhancing gene transfection efficiency, cellular uptake, and protection against nucleic acid degradation. These liposomes have potential for clinical gene delivery due to their high gene loading capabilities and efficient endosomal escape mechanism.
Article
Chemistry, Medicinal
Ruiqi Huang, Vijay Gyanani, Shen Zhao, Yifan Lu, Xin Guo
Summary: In this study, three novel lipids were prepared and incorporated into PEGylated liposomes containing doxorubicin to enhance their activity against solid tumors. These lipids were designed to cluster with negatively charged phosphatidylethanolamine-polyethylene glycol at acidic tumor sites, thereby triggering the conversion of liposomes into pH-sensitive convertible liposomes. The pH drop resulted in increased positive surface charges, lipid phase separation, and aggregation with cell membrane-mimetic liposomes, leading to enhanced drug release and better anticancer activity against both monolayer cells and 3D multicellular spheroids.
Article
Chemistry, Physical
Catarina Costa, Isabel S. Oliveira, Joao P. N. Silva, Sandra G. Silva, Claudia Botelho, M. Luisa C. do Vale, Maria Elisabete C. D. Real Oliveira, Andreia C. Gomes, Eduardo F. Marques
Summary: The study focused on developing nanovectors for siRNA delivery based on three gemini amino acid-based surfactants combined with MO as helper lipid. The systems showed efficient complexation of siRNA and high levels of gene silencing, with minimal cytotoxicity observed. These gemini:MO systems are promising candidates as lipofection vectors for RNA interference (RNAi)-based therapies.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Review
Pharmacology & Pharmacy
Kenan Aloss, Peter Hamar
Summary: Doxorubicin (DOX) is a potent anti-cancer agent that has attracted significant research interest for its high efficacy but dose-limiting toxicities. Various strategies, including liposomes, targeted liposomes, and pH-sensitive or thermo-sensitive liposomes combined with local heating, have been explored to enhance DOX delivery to tumors. Clinical trials have shown promising results for lyso-thermosensitive liposomal DOX (LTLD), MM-302, and C225-immunoliposomal (IL)-DOX. Additionally, preclinical models have demonstrated improved anti-tumor activity with functionalized PEGylated liposomal DOX (PLD), TSLs, and PSLs. However, further investigations are needed to address issues such as fast clearance, ligand density optimization, stability, and release rate.
Article
Chemistry, Medicinal
Mani Sharma, S. S. S. S. Sudha Ambadipudi, Neeraj Kumar Chouhan, V. Lakshma Nayak, Srihari Pabbaraja, Sai Balaji Andugulapati, Ramakrishna Sistla
Summary: Therapeutically active lipids in drug delivery systems can enhance the safety and efficacy of treatment. The liposome formulation created using synthesized biologically active lipids showed additive anti-cancer effects and reduced tumorigenic potential.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Eiji Yuba, Yuna Kado, Nozomi Kasho, Atsushi Harada
Summary: Introducing cationic lipids and using pH-responsive polysaccharides with intrinsic adjuvant function can improve the cellular association of liposomes with dendritic cells and increase cytokine production. These liposomes can promote tumor suppression by improving intra-tumoral immunosuppression and inducing antigen-specific cellular immunity.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Pharmacology & Pharmacy
Zhou Lu, Gan Zongjie, Zhang Qianyu, Liu Xueyan, Wu Kexin, Chen Baoyan, Tao Ran, Ren Fang, Hu Hui, Chen Huali
Summary: Gemini surfactants (GS) have potential as non-viral vectors for gene delivery. However, their cytotoxicity and in vivo limitations have hindered their development. In this study, GS-based gene delivery systems complexed with red blood cell membranes (RBCM) and/or DOPE-PEG2000 (DP) were prepared and evaluated. The results showed that the dual incorporation of RBCM and DP significantly improved the transfection efficiency and cell viability in hepatocytes.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Medicine, Research & Experimental
Oscar Escalona-Rayo, Ye Zeng, Renzo A. Knol, Thomas J. F. Kock, Dennis Aschmann, Bram Slutter, Alexander Kros
Summary: This study examined the effects of three clinically-approved ionizable cationic lipids (ICLs) on the performance of lipid nanoparticles (LNPs) for mRNA delivery and vaccine efficacy. The results showed differences in the in vitro and in vivo performance of LNPs containing different ICLs, suggesting that in vitro data may not adequately predict their behavior in vivo.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Nanoscience & Nanotechnology
Liefeng Hu, Shuting Li, Yonggang Lv
Summary: In this study, a pH-sensitive linear poly(ortho ester) polycation was successfully prepared and used for gene transfection in neuroma cells. The polymer showed good biocompatibility and highly specified transfection efficiency on human neuroblastoma cells, with serum tolerance and deeper transfection depth. Compared with the control group, this polymer demonstrated great potential in neuroma therapy.
ACS APPLIED NANO MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Shintaro Fumoto, Tsuyoshi Yamamoto, Kazuya Okami, Yuina Maemura, Chisato Terada, Asako Yamayoshi, Koyo Nishida
Summary: Nucleic acid and genetic medicines show great potential in treating various intractable diseases. Understanding their in vivo fate is crucial for rational design and development of drugs. Plasma protein binding can be utilized to manipulate pharmacokinetics, while ligand conjugation method is widely used for controlling drug distribution in the body.
Article
Biochemistry & Molecular Biology
Sepanta Hosseinpour, Chun Xu, Laurence J. Walsh
Summary: This study investigated the impact of photobiomodulation (PBM) on cationic liposome plasmid DNA transfection efficiency and toxicity. The results showed that using 810 nm and 970 nm lasers significantly enhanced GFP transfection efficiency, while 445 nm and 685 nm lasers reduced efficiency. Treatment with 685, 810, and 970 nm lasers at 12 J maintained cell viability and prevented toxicity of cationic liposomes, supporting the concept that PBM using near infrared laser wavelengths can improve transfection efficiency and cell viability in gene therapy.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Takashi Nakamura, Taisei Nakade, Koharu Yamada, Yusuke Sato, Hideyoshi Harashima
Summary: The development of CL1H6-LNP offers a promising approach for efficient gene silencing in NK-92 cells with low cytotoxicity. The results suggest that CL1H6-LNP has the potential for delivering siRNA to NK-92 cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
C. Bellefroid, C. Reusch, A. Lechanteur, B. Evrard, F. Debacq-Chainiaux, D. Mottet, G. Piel
Summary: This study aimed to develop a suitable gene delivery formulation for topical use on skin, and found that different lipid combinations can effectively deliver plasmid DNA into skin cells. Certain formulations showed high transfection rates with limited cytotoxicity, presenting promising potential for gene therapy in treating skin diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Andrew Pardo, Tyrone J. Hogenauer, Zhefeng Cai, Julian A. Vellucci, Efrain M. Castillo, Carl W. Dirk, Andreas H. Franz, Katja Michael
Article
Biochemistry & Molecular Biology
Sravan C. Penchala, Mark R. Miller, Arindom Pal, Jin Dong, Nikhil R. Madadi, Jinghang Xie, Hyun Joo, Jerry Tsai, Patrick Batoon, Vyacheslav Samoshin, Andreas Franz, Trever Cox, Jesse Miles, William K. Chan, Miki S. Park, Mamoun M. Alhamadsheh
NATURE CHEMICAL BIOLOGY
(2015)
Article
Biochemical Research Methods
Pachai S. Moua, Alfonso Gonzalez, Kristin T. Oshiro, Vivian Tam, Zhiguo Harry Li, Jennifer Chang, Wilson Leung, Amy Yon, Der Thor, Sri Venkatram, Andreas H. Franz, Douglas D. Risser, Joan Lin-Cereghino, Geoff P. Lin-Cereghino
PROTEIN EXPRESSION AND PURIFICATION
(2016)
Article
Chemistry, Organic
Sven Hackbusch, Andreas H. Franz
TETRAHEDRON LETTERS
(2016)
Review
Biochemistry & Molecular Biology
Sven Hackbusch, Amelia Watson, Andreas H. Franz
CARBOHYDRATE RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Yu Zheng, Xin Liu, Nataliya M. Samoshina, Vyacheslav V. Samoshin, Andreas H. Franz, Xin Guo
CHEMISTRY AND PHYSICS OF LIPIDS
(2018)
Review
Biochemistry & Molecular Biology
Amelia Watson, Sven Hackbusch, Andreas H. Franz
CARBOHYDRATE RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Christopher A. Naranjo, Anita D. Jivan, Maria N. Vo, Katherine H. de Sa Campos, Jared S. Deyarmin, Ryan M. Hekman, Christina Uribe, Aaron Hang, Kai Her, Michelle M. Fong, Joyce J. Choi, Caroline Chou, Taylor R. Rabara, Gina Myers, Pachai Moua, Der Thor, Douglas D. Risser, Craig A. Vierra, Andreas H. Franz, Joan Lin-Cereghino, Geoff P. Lin-Cereghino
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Amelia Watson, Cate Simmermaker, Ei Aung, Stephen Do, Sven Hackbusch, Andreas H. Franz
Summary: The conformational preferences of isomalto-oligosaccharides were investigated using NMR and MD simulations, revealing a right-handed helical structure and a more compact structure in specific branched chains. These findings offer insights into the conformational preferences of isomalto-oligosaccharides and provide potential assistance for designing drug delivery systems or targeting the degradation of capsular polysaccharides in multi-drug resistant bacteria.
CARBOHYDRATE RESEARCH
(2021)
Article
Chemistry, Organic
Amelia Watson, Christopher Wallis, Andreas H. Franz
Article
Chemistry, Organic
Sven Hackbusch, Amelia Watson, Andreas H. Franz
Article
Chemistry, Organic
Sven Hackbusch, Andreas H. Franz
Article
Chemistry, Organic
Andreas H. Franz, Ilona Serebnitskaya, Gurbir Gudial, Christopher Wallis
Article
Chemistry, Organic
Irina A. Dotsenko, Qinliang Zhao, Andreas H. Franz, Patrick Batoon, Nataliya M. Samoshina, Vyacheslav V. Samoshin
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Saurabh Saraswat, Archana Chugh
Summary: Cell penetrating peptides (CPP) are important tools for intracellular delivery of molecules. In this study, a novel marine-derived CPP from Engraulis japonicus, called Engraulisin, was reported. Engraulisin demonstrated successful cellular uptake and selective antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA), making it a potential candidate for drug delivery.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Niu, Si Jia Chen, Jeffery B. Klauda
Summary: Macrophage membranes in the activated state are more tightly packed, exhibit increased chain order across lipid species, and form specific lipid clusters. These findings provide physiologically accurate models for future computational studies of macrophage membranes and their proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Niki Baccile, Vincent Chaleix, Ingo Hoffmann
Summary: This study measured the bending rigidity of self-assembled structures formed by a new biobased glucolipid bioamphiphile using neutron spin-echo (NSE) technology. The results showed that the bending rigidity of these structures is lower or higher compared to phospholipid membranes, providing a new theoretical basis for the application of these new molecular systems.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Alain Bolano Alvares, Pablo E. A. Rodriguez, Gerardo D. Fidelio
Summary: The behavior of amphiphilic molecules at the air/water interface was studied to evaluate the arrangement formed in a confined area. The surface properties of zwitterionic DPPC lipid and A beta(1-40) amyloid peptide in mixed films were investigated at different temperatures. DPPC undergoes a phase transition depending on temperature and lateral pressure, which allows for the study of its influence on amyloid structure formation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Lukasz Plachta, Marzena Mach, Magdalena Kowalska, Pawel Wydro
Summary: Resveratrol, a popular phytoalexin found in grapes and red wine, has beneficial effects on the cardiovascular system, exhibits antiviral, antibacterial, and antifungal properties, and may have therapeutic effects against cancer. This study investigates the interaction of resveratrol with model cell membranes, demonstrating changes in their physicochemical parameters and highlighting the role of cholesterol content in resveratrol incorporation. The findings suggest that the molecular mechanism of action of resveratrol may involve interactions with lipid rafts.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Edurne Rujas, Beatriz Apellaniz, Johana Torralba, David Andreu, Jose M. M. Caaveiro, Shixia Wang, Shan Lu, Jose L. Nieva
Summary: This study investigates the role of fusion peptide and Trp-rich membrane proximal external region in HIV-1 fusion and demonstrates that liposome-based formulations containing FP-MPER hybrid peptides can induce the production of specific neutralizing antibodies in rabbits. The results support the use of liposomes as vaccine carriers and the inclusion of lipid membranes in immunogens to elicit specific humoral responses.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Dailin Li, Dingyuan Shi, Lei Wang
Summary: G protein-gated inwardly rectifying potassium (GIRK) channels play a significant role in regulating cell excitability. This study investigated the ion permeation mechanism in GIRK2 mutants using molecular dynamic simulations and QM/MM methods. The results revealed the importance of a multi-ion distribution for ion conduction.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Max J. den Uijl, Arnold J. M. Driessen
Summary: Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. The phospholipid dependence of membrane protein insertion has remained mostly unknown. This study reveals that the insertion of the mannitol permease MtlA into the membrane depends on the presence of phosphatidylglycerol and is stimulated by phosphatidylethanolamine.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick Allen, Adam C. Smith, Vernon Benedicto, Abbas Abdulhasan, Vasanthy Narayanaswami, Enrico Tapavicza
Summary: We explore the stability, size, and structure of nanodiscs formed by the N-terminal domain of apolipoprotein E3 and a variable number of 1,2-dimyristoyl-sn-glycero-3-phosphocholine molecules. Our simulations suggest that nanodiscs containing 240 to 420 DMPC molecules are stable. The antiparallel configuration shows more protein-protein interactions and ionic contacts, as well as greater stability and rigidity compared to the parallel configuration.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick M. Keating, Nicholas P. Schifano, Xinrui Wei, Matthew Y. Kong, Jinwoo Lee
Summary: Lassa virus (LASV) infection is dependent on the fusion of its viral membrane with the host cell membrane, and our study reveals that this fusion process involves pH-dependent conformational changes in the transmembrane domain of LASV. These findings provide valuable insights into the fusion mechanism of LASV and can be utilized in the design of therapeutics to combat Lassa virus infections and prevent its potential spread.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Ashley L. Bennett, Kristen N. Cranford, Austin L. Bates, Christopher R. Sabatini, Hee-Seung Lee
Summary: This study employed molecular dynamics simulations to elucidate the binding, folding, and insertion details of TP10W in the cell membrane. The simulations revealed that TP10W can translocate across the lipid membrane as a monomer, and the charged amino acid side chains can move between lipid leaflets. Comparison with point mutated variants demonstrated significant conformational changes due to charge distribution.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Jessica H. van Wonderen, Jason C. Crack, Marcus J. Edwards, Thomas A. Clarke, Gerhard Saalbach, Carlo Martins, Julea N. Butt
Summary: Electrogenic bacteria transfer excess respiratory electrons to metal oxide particles and electrodes through the MtrCAB complex. The crystal structure of MtrCAB from S. baltica OS185 was resolved, and liquid chromatography mass spectrometry was used to determine the mass values of the three proteins in purified MtrCAB complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Review
Biochemistry & Molecular Biology
Yi-Ming Chen, Ching-Tai Lu, Chia-Wen Wang, Wolfgang B. Fischer
Summary: A series of dye ligands were ranked based on docking experiments, selecting the most suitable ligands for binding with different proteins. Additionally, a comparison study was conducted on a series of repurposing drugs and known antivirals.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)