Evidence of disulfide bond scrambling during production of an antibody-drug conjugate

Direct mass spectrometric characterization of disulfide linkages

(2018) Xiaoyan Guan et al.   mAbs

Influence of disulfide bond isoforms on drug conjugation sites in cysteine-linked IgG2 antibody-drug conjugates

(2018) Lily Liu-Shin et al.   mAbs

Strategies and challenges for the next generation of antibody–drug conjugates

(2017) Alain Beck et al.   NATURE REVIEWS DRUG DISCOVERY

Development of a sample preparation method for monitoring correct disulfide linkages of monoclonal antibodies by liquid chromatography–mass spectrometry

(2016) Yi Wang et al.   ANALYTICAL BIOCHEMISTRY

Evaluation of disulfide scrambling during the enzymatic digestion of bevacizumab at various pH values using mass spectrometry

(2016) Wang-Chou Sung et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Characterization and Higher-Order Structure Assessment of an Interchain Cysteine-Based ADC: Impact of Drug Loading and Distribution on the Mechanism of Aggregation

(2016) Jianxin Guo et al.   BIOCONJUGATE CHEMISTRY

Influence of immunoglobulin isotype on therapeutic antibody function

(2016) Stephen A. Beers et al.   BLOOD

Fast and Efficient non-reduced Lys-C digest using pressure cycling technology for antibody disulfide mapping by LC–MS

(2016) Ying Cheng et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Conformational Difference in Human IgG2 Disulfide Isoforms Revealed by Hydrogen/Deuterium Exchange Mass Spectrometry

(2015) Aming Zhang et al.   BIOCHEMISTRY

Effect of Attachment Site on Stability of Cleavable Antibody Drug Conjugates

(2015) Magdalena Dorywalska et al.   BIOCONJUGATE CHEMISTRY

Investigation of antibody disulfide reduction and re-oxidation and impact to biological activities

(2015) Tian Wang et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Characterization of Cysteine-Linked Conjugation Profiles of Immunoglobulin G1 and Immunoglobulin G2 Antibody–Drug Conjugates

(2015) Brian Wiggins et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Molecular properties of human IgG subclasses and their implications for designing therapeutic monoclonal antibodies against infectious diseases

(2015) Vashti Irani et al.   MOLECULAR IMMUNOLOGY

Antibody-Drug Conjugates: Design, Formulation and Physicochemical Stability

(2015) Satish K. Singh et al.   PHARMACEUTICAL RESEARCH

Auristatin Antibody Drug Conjugate Physical Instability and the Role of Drug Payload

(2014) Yilma T. Adem et al.   BIOCONJUGATE CHEMISTRY

Long-Term Stabilization of Maleimide–Thiol Conjugates

(2014) Shaun D. Fontaine et al.   BIOCONJUGATE CHEMISTRY

IgG Subclasses and Allotypes: From Structure to Effector Functions

(2014) Gestur Vidarsson et al.   Frontiers in Immunology

Investigation into Temperature-Induced Aggregation of an Antibody Drug Conjugate

(2013) Nia S. Beckley et al.   BIOCONJUGATE CHEMISTRY

Location Matters: Site of Conjugation Modulates Stability and Pharmacokinetics of Antibody Drug Conjugates

(2013) Pavel Strop et al.   CHEMISTRY & BIOLOGY

IgG2 disulfide isoform conversion kinetics

(2013) Yaoqing Diana Liu et al.   MOLECULAR IMMUNOLOGY

Three-Dimensional Structure of the Human Myeloma IgG2

(2013) Sergey Ryazantsev et al.   PLoS One

Kinetics and Mechanisms of Thiol–Disulfide Exchange Covering Direct Substitution and Thiol Oxidation-Mediated Pathways

(2012) Péter Nagy   ANTIOXIDANTS & REDOX SIGNALING

Isotype and glycoform selection for antibody therapeutics

(2012) Roy Jefferis   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Engineering an Improved IgG4 Molecule with Reduced Disulfide Bond Heterogeneity and Increased Fab Domain Thermal Stability

(2012) Shirley J. Peters et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Characterization and Comparison of Disulfide Linkages and Scrambling Patterns in Therapeutic Monoclonal Antibodies: Using LC-MS with Electron Transfer Dissociation

(2011) Yi Wang et al.   ANALYTICAL CHEMISTRY

Tunable Degradation of Maleimide–Thiol Adducts in Reducing Environments

(2011) Aaron D. Baldwin et al.   BIOCONJUGATE CHEMISTRY

Mechanism of Immunoglobulin G4 Fab-arm Exchange

(2011) Theo Rispens et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Disulfide Scrambling in IgG2 Monoclonal Antibodies: Insights from Molecular Dynamics Simulations

(2011) Xiaoling Wang et al.   PHARMACEUTICAL RESEARCH

Acid-Induced Aggregation of Human Monoclonal IgG1 and IgG2: Molecular Mechanism and the Effect of Solution Composition

(2010) Sanjay B. Hari et al.   BIOCHEMISTRY

Human immunoglobulin allotypes

(2010) Roy Jefferis et al.   mAbs

Stability of IgG isotypes in serum

(2010) Ivan Correia   mAbs

Intrachain disulfide bond in the core hinge region of human IgG4

(2010) James W. Bloom et al.   PROTEIN SCIENCE

Determination of Fab−Hinge Disulfide Connectivity in Structural Isoforms of a Recombinant Human Immunoglobulin G2 Antibody

(2009) Bing Zhang et al.   ANALYTICAL CHEMISTRY

The Role of Thiols and Disulfides on Protein Stability

(2009) Maulik Trivedi et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

Analysis of human antibody IgG2 domains by reversed-phase liquid chromatography and mass spectrometry

(2009) Boxu Yan et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Mass measurement and top-down HPLC/MS analysis of intact monoclonal antibodies on a hybrid linear quadrupole ion trap-orbitrap mass spectrometer

(2009) Pavel V. Bondarenko et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Human IgG2 Antibodies Display Disulfide-mediated Structural Isoforms

(2008) Jette Wypych et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural and Functional Characterization of Disulfide Isoforms of the Human IgG2 Subclass

(2008) Thomas M. Dillon et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Human IgG2 Antibody Disulfide Rearrangementin Vivo

(2008) Y. Diana Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY