Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 8, Issue 17, Pages 10775-10787Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b02145
Keywords
calcium sulfate; hydroxyapatite; bisphosphonates; biomimetic; osteoblasts; osteoinductivity; osteopromotive
Funding
- Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India
- Department of Science and Technology (DST), Ministry of Science and Technology, Government of India
- IIT Kanpur
- DBT TATA Innovation Fellowship, Government of India
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In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma. Saos-2 cells derived bone morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also incorporated to cement. In vitro studies conducted using Saos-2 demonstrated enhanced cell proliferation on gelatin (0.2%w/v) cement. The differentiation of C2C12 mouse myoblast cells into bone forming cells showed 6-fold increase in ALP. levels on gelatin cement. Polymerase chain reaction (PCR) for bone biomarkers showed osteoinductive potential of gelatin cement, We investigated efficacy for local delivery of these bioactive molecules in,enhancing bone substitution qualities of bone cements by implanting in 3.5 mm critical size defect in tibial metaphysis of wistar rats. The rats were sacrificed after 12 weeks and 16 weeks post implantation. X-ray, micro-CT, histology, and histomorphometry analysis were performed to check bone healing. The cement materials slowly resorbed; from the defect site leaving HAP creating porous matrix providing surface for bone formation. The materials showed high biocompatibility and initial bridging was observed in all the animals but maximum bone formation was observed in animals implanted with cement incorporated with zoledronic acid followed by cement with BMP's compared. to. other groups.
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