Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 140, Issue 45, Pages 15161-15165Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.8b09037
Keywords
-
Categories
Funding
- national research foundation of South Korea (NRF) grant - Korea government (MSIP
- Ministry of Science, ICT & Future Planning) [2017R1C1B2010945]
- DGIST - MSIP [18-LC-01]
- NRF - MSIP [NRF-2018R1A2B3004096]
- DGIST Undergraduate Group Research Program (UGRP) grant
- National Research Foundation of Korea [2017R1C1B2010945] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Monitoring the dynamics of proteins in live cells on appropriate spatiotemporal scales may provide key information regarding long-standing questions in molecular and cellular regulatory mechanisms. However, tools capable of imaging the conformational changes over time have been elusive. Here, we present a single-molecule stroboscopic imaging probes by developing gyroscopic plasmonic nanoparticles, allowing for replication of protein protein interactions and the conformational dynamics based on rotational and lateral velocities. This study fundamentally monitors the rotational motion of a membrane protein, epidermal growth factor receptor (EGFR), to decipher undiscovered structural dynamics in live cells without any molecular perturbations. This method offers a strategy to visualize assemblies and conformational changes, and provides unique insights into the mechanism underlying the molecular dynamics for receptors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available