Journal
JOURNAL OF RAMAN SPECTROSCOPY
Volume 49, Issue 12, Pages 2050-2052Publisher
WILEY
DOI: 10.1002/jrs.5493
Keywords
Bacillus anthracis; DCDR; dipicolinic acid; Drop coating deposition Raman
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Funding
- Charles University Research Centre [UNCE/SCI/010]
- Czech Science Foundation [18-10897S]
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Dipicolinic acid (DPA) is an important spore biomarker for Bacillus anthracis (anthrax). Drop coating deposition Raman (DCDR) spectroscopy on a commercial hydrophobic SpectRIM (TM) plate allowed DPA to be detected at 5 x 10(-7)-M deposited concentration, equivalent to approximately 90 spores, significantly below the infective dose (10(4) spores). The DCDR spectra of DPA differ from Raman spectrum of DPA powder, crystalline state, and aqueous solution.
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