Article
Genetics & Heredity
Jianbo Song, Hanyi Mao, Jie Cheng, Yong Zhou, Rongrong Chen, Liming Zeng, Hua Li, Yihua Wang
Summary: This study identified 11 MtTPS genes in the genome of Medicago truncatula, divided into two subfamilies, and found that five of them could be induced by drought, salt or cold stresses, providing a foundation for further research on the regulatory function of TPS genes in response to abiotic stresses.
Article
Plant Sciences
Liugen Xu, Yong Zhou, Jie Cheng, Liqing Kang, Yuqi Qiang, Xuemei Yan, Yuhua Yan, Ying Tang, Yihua Wang, Hua Li, Jianbo Song
Summary: Thioredoxin (Trx) is a heat-stable protein involved in reduction reactions, with 46 MtTrx genes identified in Medicago truncatula and shown to play important roles in various tissues and stress responses.
ACTA PHYSIOLOGIAE PLANTARUM
(2022)
Article
Biotechnology & Applied Microbiology
Qian Li, Wenxuan Du, Xinge Tian, Wenbo Jiang, Bo Zhang, Yuxiang Wang, Yongzhen Pang
Summary: The HAK gene family plays a vital role in ion uptake and stress responses in plants. This study identified 22 HAK genes in each Medicago genome and found that they exhibited distinct expression patterns in response to salt and drought treatments. The presence of defense and stress-related cis-acting elements in their promoter regions suggests their involvement in responses to various abiotic stresses.
Review
Plant Sciences
Xiuxiu Zhang, Yu Sun, Xiao Qiu, Hai Lu, Inhwan Hwang, Tianzuo Wang
Summary: Legume plants are important food sources, but their production is limited by abiotic stresses. This review provides a comprehensive summary of the adaptive mechanism of the model legume plant Medicago truncatula in response to drought, salt, and cold stress, and discusses future research directions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Wenxuan Du, Junfeng Yang, Qian Li, Qian Su, Dengxia Yi, Yongzhen Pang
Summary: Growth Regulatory Factors (GRF) are plant-specific transcription factors that play critical roles in plant growth and development as well as plant tolerance against stress. In this study, 16 GRF genes were identified from the genomes of Medicago truncatula and Medicago sativa, and their sequences and expression patterns were analyzed. The results showed that these GRF genes are important for plant growth and stress tolerance, particularly in response to salt and drought stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wenxuan Du, Junfeng Yang, Qian Li, Chunfeng He, Yongzhen Pang
Summary: Nuclear factor YB (NF-YB) genes are critical regulators in plant growth, development, and stress resistance. In this study, 49 NF-YB genes were identified in Medicago, and their expression levels were found to be induced by drought and salt treatments. The study provides valuable information for functional characterization and application of NF-YB genes in alfalfa breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Weiyi Kong, Jia Shi, Bo Yang, Shuhan Yu, Pengcheng Zhao, Zhenfei Guo, Haifeng Zhu
Summary: In this study, the researchers identified and characterized 54 MtWAK genes in Medicago truncatula. These genes were distributed across all chromosomes, with a concentration on chromosomes 1 and 3. Comparison with other plant species revealed orthologous pairs between M. truncatula and Arabidopsis thaliana, as well as Glycine max. The expression patterns of MtWAK genes varied in different tissues, developmental stages, and biotic and abiotic stresses. The study provides insights into the molecular functions of MtWAKs in plant growth, development, and stress tolerance.
Article
Plant Sciences
Jie Cheng, Songsong Zhou, Kun Yang, Hongyang Yu, Rongrong Chen, Liming Zeng, Hua Li, Yihua Wang, Jianbo Song
Summary: In this study, 170 putative RNA helicase genes were identified in the M. truncatula genome, classified into three subfamilies and shown to contain conserved functional domains. Chromosomal mapping and synteny analyses revealed tandem and segment duplications of these genes. Transcriptome and real-time PCR analysis demonstrated that the expression of 35 RNA helicase genes was affected by abiotic stress, with some genes responding to multiple stress types.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Plant Sciences
Junfeng Yang, Lin Ma, Wenbo Jiang, Yu Yao, Yuhong Tang, Yongzhen Pang
Summary: This study identified and classified 51 BGLU genes in the model legume plant Medicago truncatula, revealing their distinct expression patterns in response to various abiotic stresses and hormonal cues. The results suggest that BGLU genes play crucial roles in plant physiological processes by activating phytohormones and defense compounds in response to stressors. Further analysis indicated conserved motifs and dual targeting of MtBGLU proteins to vacuole and/or chloroplast, as well as identification of regulatory elements related to stress and phytohormones.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Shuhan Yu, Qiguo Sun, Jiaxuan Wu, Pengcheng Zhao, Yanmei Sun, Zhenfei Guo
Summary: Short-chain dehydrogenase/reductase (SDR) belongs to the NAD(P)(H)-dependent oxidoreductase superfamily and participates in diverse metabolisms. In Medicago truncatula, 213 MtSDR genes were identified, categorized into seven subgroups based on phylogenetic analysis, and divided into three types based on the cofactor-binding site and active site. Many MtSDRs show tissue-specific expression and contain cis-acting elements responsive to plant hormones and stresses in their promoters. Genes from certain MtSDR families are responsive to drought, salt, and cold, providing valuable information for future investigation on the biological functions of MtSDRs, especially in abiotic stress adaptation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Wenxuan Du, Junfeng Yang, Lin Ma, Qian Su, Yongzhen Pang
Summary: This study identified 23 CBL and 58 CIPK genes in Medicago sativa and Medicago truncatula, and classified them into different groups through phylogenetic analysis. The research revealed distinct expression patterns of these genes under various stress conditions, especially in response to drought, salt, and abscisic acid treatments. The findings suggested that CBL and CIPK genes play crucial roles in response to various abiotic stresses in Medicago.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xiaoxi Zhu, Andong Yu, Yingying Zhang, Qianwen Yu, Ruicai Long, Junmei Kang, Qingchuan Yang, Changhong Guo, Mingna Li
Summary: This study provides a comprehensive analysis of the FtsH gene family in Medicago truncatula, laying a foundation for future functional investigations. The findings also expand the potential candidate genes for improving abiotic stress tolerance in legume crops.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Xiao Li, Yiyao Hou, Fan Zhang, Mingna Li, Fengyan Yi, Junmei Kang, Qingchuan Yang, Ruicai Long
Summary: In this study, the HD-ZIP I genes in Medicago truncatula were identified and characterized, revealing their crucial roles in plant development and response to abiotic stresses. The findings provide a valuable reference for further research and contribute to the understanding of the evolution and regulatory mechanisms of the HD-ZIP I gene family.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Plant Sciences
Tao Guo, Shumin Wang, Tiejun Zhang, Lixin Xu, Yinruizhi Li, Yuehui Chao, Liebao Han
Summary: The study identified the MtDof32 gene in plants, revealing its novel functions in growth and development regulation as well as stress responses. Transgenic plants exhibited unique phenotypes such as reduced branch number and increased rosettes number, which had not been observed in previous studies.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Alvaro Lopez-Zaplana, Nicolas Martinez-Garcia, Micaela Carvajal, Gloria Barzana
Summary: This study investigated the expression levels of aquaporins in melon plants under various abiotic stresses and showed that these expression levels are related to plant water relations and nutrient levels.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Xueyang Min, Xiaoshan Lin, Boniface Ndayambaza, Yanrong Wang, Wenxian Liu
Article
Biotechnology & Applied Microbiology
Gisele Kanzana, Yufei Zhang, Tiantian Ma, Wenxian Liu, Fan Wu, Qi Yan, Xueyang Min, Zhuanzhuan Yan, Blaise Pascal Muvunyi, Jie Li, Zhengshe Zhang, Yufeng Zhao, Jiyu Zhang
JOURNAL OF APPLIED GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Qiuxia Wang, Na Wei, Xiaoyu Jin, Xueyang Min, Yitong Ma, Wenxian Liu
Summary: This study identified and characterized the MsCOPT gene family in alfalfa, revealing their high transcription levels in roots and nodules which may play crucial roles in copper absorption and transport. Analysis of gene structure indicated that chromosomal and tandem duplications contributed to the evolution of these genes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agricultural Engineering
Chuan-Jie Zhang, Yang Gao, Changji Jiang, Li Liu, Yawen Wang, Do-Soon Kim, Jialin Yu, Liqin Yu, Feifei Li, Yi Fan, Min Chen, Youxin Zhang, Xueyang Min, Haixi Zhang, Xuebing Yan
Summary: Camelina is a suitable oilseed crop for northern China, with a relatively short growing cycle and high seed yield. The oil from camelina contains various polyunsaturated fatty acids, including DHA commonly found in aquatic ecosystems, making it an attractive crop for its unique fatty acid composition.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Agronomy
Yang Gao, Youxin Zhang, Jialin Yu, Li Liu, Yawen Wang, Jingxue Zhang, Minghui Chen, Mahoney Jonathan, Xuebing Yan, Xueyang Min, Zhenwu Wei, Chuan-Jie Zhang
Summary: This study evaluated the shade tolerance of different Medicago genotypes and identified shade tolerant candidates for intercropping. The results showed that shade significantly affected branch number and plant dry weight, but not plant height and leaf area. The annual Medicago genotype, M. polymorpha 'Jinhuacai', outperformed the perennial genotypes at all shade levels.
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2022)
Article
Genetics & Heredity
Xueyang Min, Kai Luo, Wenxian Liu, Keyou Zhou, Junyi Li, Zhenwu Wei
Summary: This study identified differentially expressed genes (DEGs) between multifoliate and trifoliate alfalfa using RNA-Seq technology, and found that SPL genes play an important role in regulating compound leaf development in alfalfa. Analysis of miR156-targeted MsSPL genes revealed their diversity and positive roles under drought and salt conditions. Furthermore, miR156/MsSPL08 was significantly suppressed in multifoliate alfalfa.
Article
Biochemistry & Molecular Biology
Xiaoshan Lin, Qiuxia Wang, Xueyang Min, Wenxian Liu, Zhipeng Liu
Summary: Common vetch is a plant with strong adaptability to abiotic stress. This study investigated the transcriptomic response of common vetch to salinity stress and identified differentially expressed genes in the leaves and roots. The findings provide insights into the salinity tolerance mechanisms of common vetch and could contribute to the development of salt-tolerant cultivars.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xueyang Min, Qiuxia Wang, Zhenwu Wei, Zhipeng Liu, Wenxian Liu
Summary: This study explored the transcriptome data of common vetch subjected to cold and drought stress in leaves and roots, and identified hub abiotic stress-responsive genes. The results showed that the response to concurrent stress is distinct from single stress, and revealed the significantly enriched categories and pathways in different tissues. Additionally, overexpression of candidate genes increased yeast tolerance to cold-drought combined stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Chuan-Jie Zhang, Yang Gao, Do-Soon Kim, Jialin Yu, Changji Jiang, Yawen Wang, Youxin Zhang, Jixing Diao, Ning Wu, Min Chen, Xueyang Min, Zhao Chen, Shengnan Sun, Zhenwu Wei, Xuebing Yan
Summary: This study investigated the difference in the formation of alfalfa pollen cloud density caused by honeybees and bumblebees, and their indirect effects on wind-blown pollen dispersal. The results showed that bumblebees triggered more flowers and generated a higher pollen cloud density compared to honeybees. The wind direction and distance also affected the pollen density.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2022)
Article
Genetics & Heredity
Junyi Li, Xueyang Min, Kai Luo, Assane Hamidou Abdoulaye, Xiaofei Zhang, Wei Huang, Rui Zhang, Yinhua Chen
Summary: In this study, a comprehensive bioinformatics analysis was performed on the MsGH3 gene family in alfalfa. The analysis revealed the presence of 31 MsGH3 genes in the alfalfa genome, which were classified into GH3-I, GH3-II, and GH3-III subgroups. Most of the MsGH3 genes showed tissue specificity and responsiveness to environmental stress-related hormones. Some of these genes, particularly MsGH3-12, MsGH3-13, and MsGH3-15, were found to play important roles in stress responses, as demonstrated by qRT-PCR results following exposure to high temperature, drought, and salt treatments.
Article
Multidisciplinary Sciences
Jia Wei, Bo Luo, Shiyi Kong, Wenxian Liu, Chuanjie Zhang, Zhenwu Wei, Xueyang Min
Summary: In this study, a comprehensive transcriptome analysis of common vetch under multiple abiotic stress treatments was conducted using hybrid-sequencing technology. Various important genetic elements, such as alternative splicing events, simple sequence repeats, and putative transcription factors, were identified. Additionally, long noncoding RNAs and complete coding sequences were also discovered. Through gene expression, annotation information, and protein-protein interaction networks, seven candidate genes responsive to multiple abiotic stresses were obtained. This research not only enriched the understanding of common vetch's transcriptome, but also provided valuable insights into the molecular mechanism of stress tolerance in this and related leguminous plants.
Article
Agronomy
Jiaqing Li, Zhenwu Wei, Xueyang Min, Peizhou Zhao, Linghua Yang, Nana Liu
Summary: Wenling Medic, a common plant in southern China, is used as a vegetable and raw ingredient. However, its seed germination rate is low and its storage life is short. The decrease in germination ability is related to higher lipid peroxidation and decreased carbohydrate levels, but is not influenced by protein concentrations or alpha-amylase activity. Careful control of dehydration timing is necessary when using osmoregulation to prime Wenling Medic seeds.
Article
Plant Sciences
Nana Liu, Zhenwu Wei, Xueyang Min, Linghua Yang, Youxin Zhang, Jiaqing Li, Yuwei Yang
Summary: In this study, 23 SWEET transporter members were identified in the Medicago polymorpha genome, divided into four subfamilies. These genes showed similar gene structure and motif composition within the same subfamily. Some genes were specifically expressed in different developmental stages or tissues, and six genes were significantly upregulated under abiotic stress treatments.
Article
Plant Sciences
Linghua Yang, Xueyang Min, Zhenwu Wei, Nana Liu, Jiaqing Li, Youxin Zhang, Yuwei Yang
Summary: This study identified 36 MpDof genes in the M. polymorpha genome and classified them into 10 groups based on comparative phylogenetic analysis. The essential information of MpDof genes, including chromosomal localization, gene structure, conserved motifs, and selective pressures, was analyzed. The MpDof genes showed diverse expression patterns in different tissues, and some genes were specifically expressed in certain organs, indicating their crucial roles in development. Expression analysis also revealed that several MpDof genes were significantly up-regulated under various stress conditions. This comprehensive analysis provides new insights into the function of Dof genes in Medicago plants.
Article
Plant Sciences
Boniface Ndayambaza, Xiaoyu Jin, Xueyang Min, Xiaoshan Lin, Xiaofan Yin, Wenxian Liu
Summary: The bHLH transcription factor is an important regulator in plant growth and development, particularly in response to stresses. This study identified and characterized a total of 159 MtbHLH and 133 MsbHLH genes in barrel medic and alfalfa, respectively, and revealed their expression patterns in drought, salt, and cold stresses. Phylogenetic analyses highlighted stress-related subfamilies VIII, IX, and X in these two species, providing insights into their evolutionary relationships and potential for molecular breeding to improve plant yield and stress tolerance.
JOURNAL OF PLANT GROWTH REGULATION
(2021)
