Article
Genetics & Heredity
Hongwei Duan, Junjie Hu, Longfei Xiao, Jianshu Lv, Yong Zhang, Xingxu Zhao
Summary: In sheep granulosa cells (GCs), the production of melatonin was inhibited by E2 through an ERA and ERB-mediated process, which also affected the expression of melatonin receptors.
Article
Reproductive Biology
Liang Huang, Lutong Zhang, Shengjie Shi, Xiaoge Zhou, Huan Yuan, Xiangrong Song, Yamei Hu, Weijun Pang, Gongshe Yang, Lei Gao, Guiyan Chu
Summary: The CLOCK gene plays a critical role in estradiol synthesis in porcine granulosa cells. The alteration of the CLOCK gene leads to impaired mitochondrial function and reduced estradiol synthesis.
Article
Agriculture, Dairy & Animal Science
Ling Yang, Zimo Zhao, Maosheng Cui, Leying Zhang, Qianjun Li
Summary: Heat stress negatively affects the quality and in vitro maturation of oocytes, but melatonin treatment can counteract these effects and reduce damage. Heat stress alters gene expression, while melatonin can partially restore this effect.
Article
Biochemistry & Molecular Biology
Arjoune Asma, Sirard Marc-Andre
Summary: This study found that melatonin may regulate metabolic processes during folliculogenesis by activating PKA and PKB signaling pathways, thereby slowing down follicular growth and differentiation and preventing follicular atresia and early luteinization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Yamei Hu, Junjie Xu, Sheng Jie Shi, Xiaoge Zhou, Liguang Wang, Liang Huang, Lei Gao, Weijun Pang, Gongshe Yang, Guiyan Chu
Summary: The research found that fibroblast growth factor 21 (FGF21) promotes the proliferation and estradiol (E2) synthesis in porcine ovarian granulosa cells (GCs) by activating signaling pathways.
Article
Biochemistry & Molecular Biology
Isao Tamura, Hiroshi Tamura, Mai Kawamoto-Jozaki, Yuichiro Shirafuta, Taishi Fujimura, Yumiko Doi-Tanaka, Yumiko Mihara, Toshiaki Taketani, Norihiro Sugino
Summary: Melatonin is a potential reagent for improving assisted reproductive technology (ART) outcomes in infertility patients. However, its effectiveness varies among patients, and the specific conditions under which melatonin is effective remain unclear. This study found that melatonin can increase fertilization rates and blastocyst formation rates, particularly in patients with low previous-cycle rates. Furthermore, melatonin alters the transcriptome of granulosa cells, improving oocyte quality and ultimately enhancing ART outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Reproductive Biology
Qiuzi Shen, Yu Liu, Honggang Li, Ling Zhang
Summary: Mitophagy is crucial for maintaining mitochondria homeostasis and cell survival. While aberrant mitochondria can be removed through mitophagy during early stages of oogenesis, dysfunctional mitochondria can be transmitted from oocytes to embryos when mitophagy is not actively initiated. Granulosa cells can improve mitochondrial function through mitophagy, enhancing oocyte developmental capacity. Research on substances and environmental conditions influencing mitophagy in oocytes and granulosa cells may provide new strategies for improving oocyte quality in assisted reproductive technology treatment.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Xuan Zhang, Dingding Zhang, Hongmin Li, Zhaojun Liu, Yatong Yang, Jiameng Li, Lishiyuan Tang, Jingli Tao, Honglin Liu, Ming Shen
Summary: This study reveals an innovative protective mechanism of melatonin against hypoxia-induced granulosa cell (GC) apoptosis by selectively inhibiting mitochondrial ROS (mtROS) generation. Melatonin activates MTNR1B to promote SOD2 expression, effectively suppressing mtROS-JNK-FOXO1-mediated apoptosis.
Article
Cell Biology
Seul-Gi Yang, Seung-Yeon Joe, Jin-Wook Bae, Gyeong-Deok Heo, Hyo-Jin Park, Deog-Bon Koo
Summary: The study found a close relationship between mitochondrial function and oocyte maturation in pigs, with melatonin being able to rehabilitate meiotic maturation disrupted by Mdivi-1. Melatonin also has direct beneficial effects on meiotic maturation through tubulin formation factors during porcine oocyte maturation, reducing DNA damage and improving spindle assembly.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Xinyuan Zhu, Shanshan Zhao, Shibo Xu, Dongyu Zhang, Minghui Zhu, Qingjie Pan, Jiaojiao Huang
Summary: Assisted reproductive technology has significant clinical applications and commercial values in the horse industry. This study found that co-culture with granulosa cells (GCs) improves the cytoplasmic maturation of mare oocytes. The increased expression of COL1A2, COL6A1, and COL6A2 is involved in this improvement through the regulation of collagen.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Liang Huang, Huan Yuan, Shengjie Shi, Xiangrong Song, Lutong Zhang, Xiaoge Zhou, Lei Gao, Weijun Pang, Gongshe Yang, Guiyan Chu
Summary: The CLOCK protein was found to inhibit the proliferation of porcine ovarian granulosa cells by increasing ASB9 level.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Reproductive Biology
Ke Hu, Chen Wang, Yifan Xu, Fan Li, Xuefeng Han, Chuanwang Song, Meng Liang
Summary: The functions and mechanisms of lncRNAs in reproduction are extensively studied, but the regulation of hormone synthesis in ovarian follicular granulosa cells by lncRNAs is not well understood. This study identified the interaction between lncRNA Gm2044 and the EEF2 protein, and demonstrated that lncRNA Gm2044 promotes estradiol synthesis in follicular granulosa cells. Knockout mice experiments further confirmed the role of lncRNA Gm2044 in regulating estradiol synthesis. Understanding the mechanism of lncRNA Gm2044/EEF2 protein in estradiol synthesis can have implications for treating estrogen-related reproductive diseases.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Shuaifei Song, Wenfei Ding, Hui Yao, Lei Wang, Bijun Li, Yukun Wang, Xue Tang, Yiyu Zhang, Deli Huang, Dejun Xu, Zhongquan Zhao
Summary: The findings showed that BMP6 had no significant effects on the proliferation, cell cycle, and apoptosis of goat ovarian granulosa cells. However, it did increase the secretion of 17 beta-estradiol (E2) and progesterone (P4) by upregulating the mRNA expression of CYP19A1 and CYP11A1.
Article
Cell Biology
Jung-Chien Cheng, Lanlan Fang, Yang Yan, Jingyan He, Yanjie Guo, Qiongqiong Jia, Yibo Gao, Xiaoyu Han, Ying-Pu Sun
Summary: Estradiol, a main steroid hormone secreted by the ovaries, is crucial for maintaining normal female reproductive function;TGF-beta1 has been shown to stimulate aromatase expression and E2 production in ovarian granulosa cells through the involvement of SMAD2 and ERK1/2 signaling pathways.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
G. D. Catandi, D. R. Bresnahan, S. O. Peters, K. J. Fresa, L. J. Maclellan, C. D. Broeckling, E. M. Carnevale
Summary: Many age-related changes in metabolite abundance suggest impaired mitochondrial metabolic function and oxidative stress in oocytes and follicular cells. The results also indicate impaired transfer of carbohydrate and free fatty acid substrates from cumulus cells to the oocytes of old mares, potentially related to disruption of transzonal projections between the cell types. The identification of age-associated alterations in the abundance of specific metabolites and their correlations among cells contribute to our understanding of follicular dysfunction with maternal aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)