Article
Immunology
Arpa Aintablian, Sandra Strozniak, Marion Heuer, Manfred B. Lutz
Summary: Myeloid-derived suppressor cells (MDSC) are major regulators of immune responses, controlling T cells via their inducible nitric oxide synthase (iNOS) and arginase 1 (Arg1). IL-3, similar to GM-CSF, can generate monocytic MDSC (M-MDSC) with increased Arg1 expression and Arg1-dependent suppression.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Hong-Wei Sun, Wen-Chao Wu, Hai-Tian Chen, Yi-Tuo Xu, Yan-Yan Yang, Jing Chen, Xing-Juan Yu, Zilian Wang, Ze-Yu Shuang, Limin Zheng
Summary: The study demonstrates that glutamine deprivation can enhance the expression of G-CSF and GM-CSF in breast cancer cells, leading to generation of immunosuppressive myeloid-derived suppressor cells (MDSCs) due to impaired HPC-maintaining capacity in the bone marrow.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Qianyue Zhang, Qiaoling Song, Shan Liu, Yuting Xu, Danling Gao, Peizhe Lu, Yuantao Liu, Guanghui Zhao, Lihong Wu, Chenyang Zhao, Jinbo Yang
Summary: This study investigated the metabolic differences between GM-BMDMs and M-BMDMs and their consistency with protein levels through transcriptome and metabolome analysis. The core metabolic pathways of macrophages were found to be closely associated with canonical macrophage functions such as inflammatory response and phagocytosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Xiu Teng, Li-Ping Li, Meng Tang, Hui-Fang Li, Xiang-Yi Ren, Ling Jiao, Sha-Sha Wu, Da-Chao Mou, Zhi-Yong Miao
Summary: This study induced a novel T cell subset called Th-GM cells in a mouse CHS model and found that they were mainly expanded at the site of injury and draining lymph nodes. This method can be used to further investigate the biology of Th-GM cells and explore therapeutic strategies centered on GM-CSF in various conditions.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Immunology
Seul Hye Ryu, Hyun Soo Shin, Hye Hyeon Eum, Ji Soo Park, Wanho Choi, Hye Young Na, Hyunju In, Tae-Gyun Kim, Sejung Park, Soomin Hwang, Moah Sohn, Eun-Do Kim, Kyoung Yul Seo, Hae-Ock Lee, Min-Geol Lee, Min Kyung Chu, Chae Gyu Park
Summary: Dendritic cells (DCs) play a crucial role in initiating adaptive immunity. Injecting granulocyte macrophage-colony stimulating factor (GM-CSF) can generate a distinct subset of DCs called GMiDCs in the spleen, which have superior antigen-presenting ability and can activate CD4(+) T cells and polarize Th2 cells. The generation of GMiDCs depends on the expression of GM-CSF receptor, and they can also be found in other tissues and during chronic allergic inflammation. This study provides important insights into the development and immune regulation of DCs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Lucia Minarrieta, Gloria J. Godoy, Lis N. Velazquez, Peyman Ghorbani, Tim Sparwasser, Luciana Berod
Summary: The CD11c(+)MHCII(+) compartment in GM-CSF cultures contains two populations with different metabolic profiles: MHCII(low)CD11b(high) cells (GM-Macs) and MHCII(high)CD11b(int) cells (GM-DCs). GM-Macs upregulate iNOS and produce nitric oxide (NO) to inhibit mitochondrial respiration (OXPHOS), while promoting glycolytic metabolism in GM-DCs, which do not express iNOS naturally.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Tatsuma Honzawa, Kazuhiko Matsuo, Shunya Hosokawa, Mayu Kamimura, Yuichiro Kaibori, Yuta Hara, Daisuke Nagakubo, Naoki Oiso, Akira Kawada, Atsushi Otsuka, Osamu Yoshie, Takashi Nakayama
Summary: This study investigated the role of CCR4 in collagen-induced arthritis (CIA) and found that CCR4 plays a critical role in the pathogenesis of CIA and may represent a new therapeutic target for rheumatoid arthritis (RA).
INTERNATIONAL IMMUNOLOGY
(2022)
Article
Allergy
Samuel Philip Nobs, Lea Pohlmeier, Fengqi Li, Merve Kayhan, Burkhard Becher, Manfred Kopf
Summary: Through experimental animal models, it was found that the GM-CSF signaling pathway plays a key role in regulating the accumulation of neutrophils in the lungs in chronic asthma, thereby promoting the development of airway hyperresponsiveness in chronic diseases.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Hyunju In, Ji Soo Park, Hyun Soo Shin, Seul Hye Ryu, Moah Sohn, Wanho Choi, Sejung Park, Soomin Hwang, Jeyun Park, Lihua Che, Tae-Gyun Kim, Min Kyung Chu, Hye Young Na, Chae Gyu Park
Summary: DCs can be generated from mouse bone marrow with GM-CSF or FLT3L treatment, and can be classified into different subsets based on marker expression and antibody staining. In FLT3L-treated cultures, a majority of cDC2-gated CD11c(+)MHCII(hi) cells exhibited a specific phenotype, suggesting a new cDC precursor population.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Annette D. D. Wagner, Ulrike Wittkop, Jessica Thalmann, Tina Willmen, Vega Goedecke, Justyna Hodam, Simon Ronicke, Martin Zenke
Summary: In patients with GCA, glucocorticoid therapy leads to a rapid reduction in the number of DCs, an increase in apoptotic cells, and a decrease in the expression of mediators for cell migration. These findings suggest GM-CSF as a potential therapeutic target for GCA.
FRONTIERS IN MEDICINE
(2021)
Article
Cell Biology
Shannon E. Gilchrist, Grace M. Pennelli, Sassan Hafizi
Summary: The study reveals that Gas6 regulates the inflammatory response of CNS glial cells to LPS by inhibiting Csf2 gene induction and NF-kappa B signaling pathway. Microglia are identified as the major cellular source of GM-CSF in neuroinflammatory response.
Article
Medicine, Research & Experimental
Adam L. Burrack, Zoe C. Schmiechen, Michael T. Patterson, Ebony A. Miller, Ellen J. Spartz, Meagan R. Rollins, Jackson F. Raynor, Jason S. Mitchell, Tsuneyasu Kaisho, Brian T. Fife, Ingunn M. Stromnes
Summary: We investigated the differential impact of myeloid subsets on immunity to pancreatic ductal adenocarcinoma (PDA). Our study demonstrates that tumor antigenicity shapes the composition and functionality of myeloid cells. The therapeutic effectiveness of adoptive T cell therapy or programmed cell death ligand 1 blockade relies on the presence of type 1 dendritic cells (cDC1), which support the survival and function of antitumor T cells. Our research highlights the essential role of cDC1s in sustaining effective antitumor T cells and reveals differential roles for cDC1s and monocytes/macrophages in guiding T cell fate and immunotherapy response.
Article
Cell & Tissue Engineering
Shuhei Kobashi, Tomoya Terashima, Miwako Katagi, Makoto Urushitani, Hideto Kojima
Summary: The study demonstrates that intraspinal transplantation of bone marrow-derived inducible microglia-like (BM-iMG) cells improves motor function, prolongs survival, and reduces neuronal cell death and inflammation in ALS model mice.
Article
Pediatrics
Fook-Choe Cheah, Pietro Presicce, Tian-Lee Tan, Brenna C. Carey, Suhas G. Kallapur
Summary: The study found that exposure to GM-CSF antenatally results in accumulation of immature macrophages in the fetal lung interstitium. Lack of GM-CSF did not eliminate but delayed the transitioning of interstitial macrophages. These results suggest that other perinatal factors may play a role in modulating the maturation of alveolar macrophages in the developing fetal lung.
FRONTIERS IN PEDIATRICS
(2021)
Article
Immunology
Ee Shan Pang, Ghazal Daraj, Katherine R. Balka, Dominic De Nardo, Christophe Macri, Hubertus Hochrein, Kelly-Anne Masterman, Peck S. Tan, Angus Shoppee, Zoe Magill, Nazneen Jahan, Mariam Bafit, Yifan Zhan, Benjamin T. Kile, Kate E. Lawlor, Kristen J. Radford, Mark D. Wright, Meredith O'Keeffe
Summary: This study compared the responses of mouse and human plasmacytoid DCs and conventional DC subsets upon STING activation. It was found that both mouse and human DC subsets were activated by STING stimulation and produced Type I interferons. However, only human DCs produced large amounts of IFN-lambda 1, which is not expressed in the mouse genome. Additionally, mouse plasmacytoid DCs underwent rapid cell death upon STING activation, while human plasmacytoid DCs did not.
FRONTIERS IN IMMUNOLOGY
(2022)