Journal
JOURNAL OF CELLULAR PHYSIOLOGY
Volume 234, Issue 3, Pages 2491-2499Publisher
WILEY
DOI: 10.1002/jcp.26776
Keywords
iTRAQ; osteogenic differentiation; SEMA3B-AS1
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Funding
- National Natural Science Foundation of China [81660156, 81760136]
- Joint special fund of Applied Fundamental Research of Kunming Medical University - Science and Technology Offce of Yunnan [2014FZ048, 2017FE468(-181)]
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Human mesenchymal stem cells (hMSCs) are fibroblastoid multipotent adult stem cells with capacities of differentiation into osteoblasts and chondrocytes and show great potential in new bone formation and bone repair-related clinical settings, such as osteoporosis. Long noncoding RNAs (lncRNAs) have been demonstrated to play important roles in various biological processes. Here, we report an antisense lncRNA SEMA3B-AS1 regulating hMSCs osteogenesis. SEMA3B-AS1 is proximal to a member of the semaphorin family Sema3b. Overexpression of SEMA3B-AS1 using the lentivirus system markedly inhibits the proliferation of hMSCs and meanwhile reduces osteogenic differentiation. Using a comprehensive proteomic technique named isobaric tag for relative and absolute quantitation, we found that SEMA3B-AS1 significantly alters the process of osteogenesis through downregulating the expression of proteins involved in actin cytoskeleton, focal adhesion, and extracellular matrix-receptor interaction, while increasing the expression of proteins in the spliceosome. Collectively, we find that SEMA3B-AS1 is a target for controlling osteogenesis of hMSCs.
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