Article
Biochemistry & Molecular Biology
Xiaoyu Lu, Min Yang, Shengwen Zhou, Shuo Yang, Xiran Chen, Mehwish Khalid, Kexin Wang, Yaqun Fang, Chaoming Wang, Ren Lai, Zilei Duan
Summary: Staphylococcus aureus (S. aureus) infections are a leading cause of morbidity and mortality, and drug resistance worsens their severity. Researchers have discovered a new antimicrobial peptide called RK22 that exhibits good antibacterial activity against S. aureus, rapidly killing the bacteria without exacerbating the coagulation system. In in vivo experiments, RK22 significantly inhibited S. aureus infection, including a clinically resistant strain, making it a potential candidate for the treatment of S. aureus infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jonggwan Park, Hyeongsun Kim, Hee-Kyoung Kang, Moon-Chang Choi, Yoonkyung Park
Summary: Lycosin-II, an antimicrobial peptide, exhibits antifungal activity against Candida albicans by damaging the fungal membrane and inducing oxidative stress. It also inhibits dual-species biofilm formation of Candida albicans and Staphylococcus aureus.
Article
Microbiology
Diana Gutierrez, Lorena Rodriguez-Rubio, Patricia Ruas-Madiedo, Lucia Fernandez, Ana Belen Campelo, Yves Briers, Martin Weiss Nielsen, Karl Pedersen, Rob Lavigne, Pilar Garcia, Ana Rodriguez
Summary: A series of engineered proteins based on endolysins were designed and screened, with LysRODI Delta Ami identified as having high lytic activity, stability, and antimicrobial properties to effectively prevent and treat Staphylococcus aureus skin infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Tingting Hong, Lin Qiu, Wen Rui, Ying Li, Ronghui Zheng, Qianqian Guo, Shuwen Zhou, Pengfei Cui, Jingyu Sheng, Xinye Ni, Jianhao Wang, Pengju Jiang
Summary: A novel peptide containing antimicrobial sequence and gelatinase cleavage sites was designed for Staphylococcus aureus detection. The results showed that capillary electrophoresis was efficient for determining Staphylococcus aureus content, with higher efficiency, enhanced sensitivity, and low sample consumption compared to traditional approaches. Moreover, the proposed peptide also exhibited desirable antimicrobial activity, indicating a new path for Staphylococcus aureus detection by capillary electrophoretic method.
Article
Immunology
Marco Lebtig, Jasmin Scheurer, Marie Muenkel, Janna Becker, Effie Bastounis, Andreas Peschel, Dorothee Kretschmer
Summary: Keratinocytes play an important role in protecting the skin barrier by producing inflammatory modulators. Activation of the FPR2 receptor in keratinocytes promotes inflammation and cell proliferation, which is necessary for eliminating bacteria like Staphylococcus aureus from the skin.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Darya V. Poshvina, Diana S. Dilbaryan, Alexey S. Vasilchenko
Summary: Antibiotic resistance is a significant issue in the medical field, and Staphylococcus aureus poses a serious threat as it is responsible for many infections and has high mortality rates. We studied the gene expression of gausemycin A-resistant S. aureus and found that changes in the cell wall and cell membrane are crucial for bacterial resistance. In the late-exponential phase, genes involved in cell wall turnover, membrane charge, phospholipid metabolism, stress response, and proteolytic system showed increased expression, while in the stationary phase, genes involved in phospholipid metabolism and proteolytic system showed decreased expression.
Article
Agriculture, Multidisciplinary
Yanan Shi, Yufang Li, Kun Yang, Guangqiang Wei, Aixiang Huang
Summary: In this study, the antimicrobial peptide BCp12 was shown to significantly suppress bacterial growth, induce cell apoptosis, and modulate malonylation levels in Staphylococcus aureus cells. Through multiple analyses, it was revealed that BCp12 treatment led to changes in malonylated proteins and metabolites, particularly in the arginine synthesis pathway. The integration of lysine malonylation and metabolomic data provided new insights into the mechanism by which BCp12 inhibits S. aureus.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Zhiyuan Huang, Wenming Dong, Jiangping Fan, Yang Tian, Aixiang Huang, Xuefeng Wang
Summary: Our study investigated the antimicrobial mechanism of the peptide MOp2 from Moringa oleifera seeds against Staphylococcus aureus using TMT-based proteomics technology. We identified differentially expressed proteins involved in inositol phosphate metabolism, cell wall synthesis, and energy metabolism, which resulted in membrane damage, oxidative stress, and cell death in S. aureus. Molecular docking further showed that MOp2 interacted with multiple proteins, confirming its multi-targeted mechanism. These findings provide a theoretical basis for the application of MOp2 as a novel food preservative and antibiotic substitute.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Zijin Yuan, Jude Juventus Aweya, Jian Li, Zhongyan Wang, Shiying Huang, Mingjing Zheng, Linfan Shi, Shanggui Deng, Shen Yang
Summary: This study identified a novel antimicrobial peptide, LCMHC, from the myosin heavy chain of Larimichthys crocea and found that it exhibited strong antibacterial activity against Staphylococcus aureus when combined with low-intensity ultrasound treatment. The peptide was able to improve the cell membrane permeability of S. aureus and assist in the binding of DNA when combined with ultrasound.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Review
Microbiology
Sahana Vasudevan, Helma David, Lakshmi Chanemougam, Jayalakshmi Ramani, Maanasa Ramesh Sangeetha, Adline Princy Solomon
Summary: A stable but reversible phenotype switch from normal to persister state is advantageous to the intracellular pathogens to cause recurrent infections and to evade the host immune system. Staphylococcus aureus is a versatile opportunistic pathogen known to cause chronic infections with significant mortality. One of the notable features is the ability to switch to a per-sisters cell, which is found in planktonic and biofilm states. This review attempts to provide a constructed argument discussing the most widely accepted mechanisms and identifying the various attributes of persistence.
CRITICAL REVIEWS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jose Geraldo de Alencar Santos Junior, Henrique Douglas Melo Coutinho, Joao Pedro Viana Rodrigues, Vanessa Pinheiro Goncalves Ferreira, Jose Bezerra de Araujo Neto, Maria Milene Costa da Silva, Ana Carolina Justino de Araujo, Raimundo Luiz Silva Pereira, Pedro Everson Alexandre de Aquino, Cicera Datiane de Morais Oliveira-Tintino, Roberto Nicolete
Summary: Liposomes can enhance the efficacy of antibiotics, reduce toxicity, and increase selectivity. This study aims to raise awareness about the need to address bacterial resistance to antibiotics and explore the potential of antibiotics carried by liposomes. The experimental results indicate that liposomes associated with antibiotics have a better growth inhibition effect on strains containing the NorA gene compared to the wild type strain.
CHEMISTRY AND PHYSICS OF LIPIDS
(2022)
Article
Biochemistry & Molecular Biology
Wan Yang, Vijay Singh Gondil, Dehua Luo, Jin He, Hongping Wei, Hang Yang
Summary: The study involves incorporating silica-binding peptide and anti-staphylococcal lysin into functional coatings to kill Staphylococcus aureus and prevent biofilm formation. SiBP1-ClyF-functionalized coatings effectively kill MRSA strains and support normal growth of mammalian cells on surfaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marcela Manrique-Moreno, Mario Suwalsky, Edwin Patino-Gonzalez, Estefania Fandino-Devia, Magorzata Jemiola-Rzeminska, Kazimierz Strzalka
Summary: The study demonstrated the antimicrobial activity of the novel synthetic peptide Delta M3, which interacts mainly with the inner monolayer of erythrocyte membrane rich in anionic lipids. The results suggest that Delta M3 is a non-cytotoxic peptide with antibacterial activity.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Veterinary Sciences
Chunling Zhu, Yaya Zhao, Xueqin Zhao, Shanqin Liu, Xiaojing Xia, Shouping Zhang, Yimin Wang, Huihui Zhang, Yanzhao Xu, Shijun Chen, Jinqing Jiang, Yundi Wu, Xilong Wu, Gaiping Zhang, Yueyu Bai, Jianhe Hu, Hanna Fotina, Lei Wang, Xueming Zhang
Summary: This study explores the antibacterial activity and mechanism of an antibacterial peptide called MPX extracted from wasp venom against Staphylococcus aureus. MPX exhibits potent bactericidal activity against S. aureus, with a minimum inhibitory concentration of 0.08 mu M. It can destroy the integrity of the bacterial membrane, increase permeability, disrupt membrane potential, and cause leakage of cellular content, resulting in bactericidal effects. In a mouse scratch model, MPX inhibits colonization by S. aureus, reduces wound size, inflammation, and promotes wound healing.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Des Field, Kiera Considine, Paula M. O'Connor, R. Paul Ross, Colin Hill, Paul D. Cotter
Summary: This article discusses the application and improvement of nisin in the treatment of bovine mastitis. Through bioengineering strategies, the specific activity of nisin against pathogens has been enhanced, and three nisin derivatives with improved activity have been proposed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Parasitology
Atul A. Chaudhari, Youngsub Lee, Hyun S. Lillehoj
VETERINARY PARASITOLOGY
(2020)
Article
Immunology
Tesfaye Belay, Rajnish Sahu, Elisha Martin, Kristin Brown, Courtney Rolen, Shomonique Hankins, Brianna Punturi, Maya Patterson, Chase Chambers, Brandon Kirby, Jonathan Butchar
Summary: The study showed that oral feeding of AHCC in stressed mice reduced shedding of C. muridarum, decreased plasma catecholamines, promoted immune cell activation and cytokine production, and enhanced clearance of C. muridarum infection in the murine stress model.
INFECTION AND IMMUNITY
(2021)
Article
Environmental Sciences
Timothy E. Egbo, Alexander Johs, Rajnish Sahu, Yazeed Abdelmageed, Jeffrey Ogbudu, Boakai K. Robertson
Summary: The study evaluates the impact of Serratia marcescens and Burkholderia thailandensis on the immobilization of mercury by sorbents, finding that common soil bacteria do not significantly affect mercury sorption by sorbent amendments. This suggests that sorbent amendments are an effective strategy for remediating mercury contamination in natural ecosystems.
WATER AIR AND SOIL POLLUTION
(2021)
Article
Virology
Richard S. Bennett, Elena N. Postnikova, Janie Liang, Robin Gross, Steven Mazur, Saurabh Dixit, Gregory Kocher, Shuiqing Yu, Shalamar Georgia-Clark, Dawn Gerhardt, Yingyun Cai, Lindsay Marron, Vladimir V. Lukin, Michael R. Holbrook
Summary: As the SARS-CoV-2 pandemic escalated, the need for effective neutralizing antibody testing became evident for the development of plasma-based therapies. By modifying and optimizing an existing fluorescence reduction neutralization assay, a high-quality neutralization assay for SARS-CoV-2 was achieved, allowing for quantitative assessment of infected cells and proficient screening of samples.
Article
Immunology
Rajnish Sahu, Saurabh Dixit, Richa Verma, Skyla A. Duncan, Lula Smith, Guillermo H. Giambartolomei, Shree R. Singh, Vida A. Dennis
Summary: The PLGA-rMOMP nanovaccine demonstrated protective efficacy in mice against genital Chlamydia challenges by enhancing immune responses and inducing specific Th1 cytokines, antibodies, and T-cell phenotypes. The vaccine also showed potential for developing a nano-based Chlamydia vaccine by triggering cellular and humoral immune effectors through extended-releasing PLGA encapsulation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Medicinal
Sameer Joshi, Rajnish Sahu, Vida A. Dennis, Shree R. Singh
Summary: The research combines alginate and montmorillonite to produce a soft non-gelatin modified-release capsule, which was critically analyzed for appearance, functionality, and durability. These capsules offer unique advantages in terms of appearance, functionality, and durability.
Review
Chemistry, Medicinal
Desh Deepak Singh, Ravi Verma, Subhash K. Tripathi, Rajnish Sahu, Poonam Trivedi, Dharmendra Kumar Yadav
Summary: Breast cancer is the second most commonly diagnosed cancer in the world, with treatment options remaining a challenge and a need for a comprehensive understanding of the interplay between genes and the development of more effective treatments. The use of CRISPR/Cas9 technology to generate BC-specific transgenic cell lines and animal models has been successfully employed, with significant potential for early BC diagnostic tools and treatments.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2021)
Article
Immunology
Shaji Daniel, Alexander Pichugin, Holly Torano, Jonathan P. Renn, Jennifer Kwan, Matthew Cowles, Solomon Conteh, Lynn E. Lambert, Nada Alani, Nicholas J. MacDonald, Weili Dai, Kendrick Highsmith, Charles Anderson, J. Patrick Gorres, Javonn Musgrove, Brandi Butler, Nouf Althubaiti, Saurabh Dixit, Stasya Zarling-Bejma, Urszula Krzych, Patrick E. Duffy
Summary: Preerythrocytic vaccines target parasites in the clinically silent sporozoite and liver stages to prevent malaria. Combining CSP with novel antigens like PEVAs may enhance sterile protective immunity.
INFECTION AND IMMUNITY
(2021)
Article
Multidisciplinary Sciences
Cherrelle Dacon, Courtney Tucker, Linghang Peng, Chang-Chun D. Lee, Ting-Hui Lin, Meng Yuan, Yu Cong, Lingshu Wang, Lauren Purser, Jazmean K. Williams, Chul-Woo Pyo, Ivan Kosik, Zhe Hu, Ming Zhao, Divya Mohan, Andrew J. R. Cooper, Mary Peterson, Jeff Skinner, Saurabh Dixit, Erin Kollins, Louis Huzella, Donna Perry, Russell Byrum, Sanae Lembirik, David Drawbaugh, Brett Eaton, Yi Zhang, Eun Sung Yang, Man Chen, Kwanyee Leung, Rona S. Weinberg, Amarendra Pegu, Daniel E. Geraghty, Edgar Davidson, Iyadh Douagi, Susan Moir, Jonathan W. Yewdell, Connie Schmaljohn, Peter D. Crompton, Michael R. Holbrook, David Nemazee, John R. Mascola, Ian A. Wilson, Joshua Tan
Summary: This study identified six monoclonal antibodies that bind to spike proteins from all seven human-infecting coronaviruses. Two of these antibodies, COV44-62 and COV44-79, showed broad neutralizing activity against alpha- and betacoronaviruses, including the Omicron subvariants of SARS-CoV-2. The fusion peptide region in the spike protein was found to be a potential candidate epitope for next-generation coronavirus vaccine development.
Review
Microbiology
Amber Grace, Rajnish Sahu, Donald R. R. Owen, Vida A. A. Dennis
Summary: Pseudomonas aeruginosa is a common pathogen that has been identified as a multi-drug resistant strain. PAO1 and PA14, two laboratory strains, have contributed significantly to our understanding of the bacterium. However, there are discrepancies between their behaviors and therapeutic susceptibilities observed in human infections. This review aims to compare the two strains and propose methods to improve their clinical relevance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Cherrelle Dacon, Linghang Peng, Ting-Hui Lin, Courtney Tucker, Chang-Chun D. Lee, Yu Cong, Lingshu Wang, Lauren Purser, Andrew J. R. Cooper, Jazmean K. Williams, Chul-Woo Pyo, Meng Yuan, Ivan Kosik, Zhe Hu, Ming Zhao, Divya Mohan, Mary Peterson, Jeff Skinner, Saurabh Dixit, Erin Kollins, Louis Huzella, Donna Perry, Russell Byrum, Sanae Lembirik, Michael Murphy, Yi Zhang, Eun Sung Yang, Man Chen, Kwanyee Leung, Rona S. Weinberg, Amarendra Pegu, Daniel E. Geraghty, Edgar Davidson, Benjamin J. Doranz, Iyadh Douagi, Susan Moir, Jonathan W. Yewdell, Connie Schmaljohn, Peter D. Crompton, John R. Mascola, Michael R. Holbrook, David Nemazee, Ian A. Wilson, Joshua Tan
Summary: In this study, 55 monoclonal antibodies were identified from COVID-19 convalescent donors that can bind diverse betacoronavirus spike proteins. Most antibodies targeted a non-neutralizing S2 epitope, but 11 antibodies targeting the stem helix showed neutralizing activity against different betacoronavirus lineages. These findings revealed a class of IGHV1-46/IGKV3-20 antibodies that can broadly neutralize betacoronaviruses by targeting the stem helix.
CELL HOST & MICROBE
(2023)
Article
Immunology
Duncan G. G. Ithinji, David W. W. Buchholz, Shahrzad Ezzatpour, I. Abrrey Monreal, Yu Cong, Julie Sahler, Amandip Singh Bangar, Brian Imbiakha, Viraj Upadhye, Janie Liang, Andrew Ma, Birgit Bradel-Tretheway, Benjamin Kaza, Yao Yu Yeo, Eun Jin Choi, Gunner P. P. Johnston, Louis Huzella, Erin Kollins, Saurabh Dixit, Shuiqing Yu, Elena Postnikova, Victoria Ortega, Avery August, Michael R. R. Holbrook, Hector C. C. Aguilar
Summary: This study demonstrates that replication-incompetent multivalent viral particle vaccines can provide protection against multiple deadly zoonotic viruses and have the potential for application in tropical regions.
Meeting Abstract
Immunology
Rajnish Sahu, Richa Verma, Homa Nath Sharma, Vanella Tadjuidje, Sameer Joshi, Othreniel A. Forte, Shree R. Singh, Vida A. Dennis
JOURNAL OF IMMUNOLOGY
(2022)