Journal
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Volume 74, Issue 2, Pages 342-348Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jac/dky451
Keywords
-
Funding
- Dennis and Mireille Gillings Foundation
- Pasteur Foundation US
- MSD AVENIR
- Monaco Department of International Cooperation
- Institut Pasteur
Ask authors/readers for more resources
Background: Salmonella enterica is a leading cause of human gastroenteritis. S. enterico strains that produce ESBLs (ESBL-Salm) remain rare in Europe and North America, but less is known about their prevalence among animal-derived foods in countries with weaker food safety practices and unregulated veterinary antibiotic use. Objectives: To examine the prevalence and characteristics of ESBL-Saim from retail meats in Phnom Penh, Cambodia, Methods: We tested fish, pork and chicken from two markets for ESBL- and carbapenemase-producing Salmonella from September-December 2016, using cefotaxime- and ertapenem-supplemented media, respectively. ESBL-Salm were sequenced and their genomes characterized. We performed plasmid conjugation experiments to assess the co-transferability of ESBL-encoding genes and MDR phenotypes. Results: Twenty-six of 150 fish and meat samples (17%) were positive for ESBL-Salm, including 10/60 fish (17%), 15/60 pork (25%) and 1/30 chicken (3%). Carbapenemase-producing Salmonella strains were not detected. Pork-origin ESBL-Salm were primarily serotypes Rissen (10/15) or a monophasic variant of Typhimurium 4,5,12:i: (3/15), whereas Saintpau( (3/10) and Newport (4/10) were more common among fish. Most ESBL enzymes were encoded by bla(CTX-M-55) genes (24/26) harboured on conjugative IncA/C2 m 14) or IncHI2 (n 10) plasmids. Resistance to up to six additional drug classes was co-transferred by each plasmid type. ESBL-Salm were resistant to almost every antibiotic recommended for severe salmonellosis treatment. Conclusions: CTX-M-55-type S. enterica are highly prevalent among pork and fish from Phnom Penh markets and their spread appears to be mediated by MDR IncA/C2 and IncHI2 plasmids. Food safety must be improved and veterinary antibiotic use should be regulated to protect public health.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available