Review
Endocrinology & Metabolism
Yun-Bo Shi
Summary: Thyroid hormone T3 plays a critical role in organ function, metabolism, and animal development, especially during the neonatal period in mammals. Studies on anuran metamorphosis in amphibians have shed light on how T3 regulates vertebrate development. The absence of T3 receptors in tadpoles leads to premature development of adult tissues and eventually lethality, highlighting the importance of TRs in mediating T3 effects.
Article
Endocrinology & Metabolism
Rikke Poulsen, Shireen H. Jackman, Martin Hansen, Caren C. Helbing
Summary: Anuran metamorphosis is associated with changes in thyroid hormone levels and gene expression. New technology allows for measuring TH levels and gene transcription during metamorphosis. The concentration profile of T2 during metamorphosis suggests its role in anuran development.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Environmental Sciences
ZhiChao Dang
Summary: Both AMA and LAGDA are used to detect thyroid-mediated modality and adversity based on morphological changes during thyroid hormone-dependent metamorphosis. The EU Guidance recommends their use for investigating the effects of thyroid hormone system disrupting chemicals. However, there is debate over the necessity of using LAGDA as a follow-up to positive AMA results. This study analyzes the similarities, differences, and sensitivity of these two assays in detecting TDCs.
ENVIRONMENTAL POLLUTION
(2022)
Article
Multidisciplinary Sciences
Pauline Salis, Natacha Roux, Delai Huang, Anna Marcionetti, Pierick Mouginot, Mathieu Reynaud, Oceane Salles, Nicolas Salamin, Benoit Pujol, David M. Parichy, Serge Planes, Vincent Laudet
Summary: It was found that the developmental timing of white bar formation in juvenile clownfish during metamorphosis is influenced by the sea anemone species they are recruited in, possibly dependent on thyroid hormone. Transcriptomic analysis revealed that higher TH levels in fish recruited in certain sea anemone species were associated with faster white bar formation, with the duox gene playing a crucial role in regulating iridophore pattern timing.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell Biology
Bidisha Paul, Zachary R. Sterner, Daniel R. Buchholz, Yun-Bo Shi, Laurent M. Sachs
Summary: Development in multicellular organisms relies on the integration of communication systems, particularly the neuroendocrine axes. The hypothalamic-pituitary-thyroid and hypothalamic-pituitary-adrenal/interrenal axes play central roles in coordinating body morphogenesis. Thyroid hormones and corticosteroids have critical functions in metamorphosis of anuran amphibians and are involved in the developmental transition seen in vertebrates.
Article
Biochemistry & Molecular Biology
Shouhong Wang, Yuki Shibata, Liezhen Fu, Yuta Tanizaki, Nga Luu, Lingyu Bao, Zhaoyi Peng, Yun-Bo Shi
Summary: Animal regeneration is a natural process that relies on the level of thyroid hormone (T3), which is responsible for the loss of regenerative ability in many organs during development. The metamorphic stages in amphibians, similar to postembryonic development in humans, provide a good model to study the gradual loss of regenerative ability and the role of T3 in this process.
CELL AND BIOSCIENCE
(2023)
Article
Cell Biology
Yuki Shibata, Yuta Tanizaki, Hongen Zhang, Hangnoh Lee, Mary Dasso, Yun-Bo Shi
Summary: Research shows that TR genes play crucial roles in larval epithelial cell death and adult stem cell formation during anuran metamorphosis. Lack of these processes in TRDKO tadpoles results in inhibition of metamorphosis progression.
Article
Environmental Sciences
Lauris Evariste, Antoine Mottier, Eric Pinelli, Emmanuel Flahaut, Laury Gauthier, Florence Mouchet
Summary: The interest in graphene-based nanomaterials (GBMs) is increasing globally due to their innovative applications, but concerns about their potential adverse effects on the environment and endocrine disruption potential are also growing. A study found that graphene oxide (GO) and reduced graphene oxide (rGO) have different effects on amphibian endocrine systems, with GBMs potentially increasing the bioavailability and toxicity of other pollutants through hormone adsorption. This highlights the need for further research on the environmental impact and potential risks associated with GBMs.
Article
Toxicology
Douglas J. Fort, M. Annegaaike Leopold, Jeffrey C. Wolf, Kevin J. Todhunter, Peter J. J. M. Weterings
Summary: The present study evaluated the impact of dietary quality on the effects of chemical stressors on premetamorphic development and metamorphosis in tadpoles. The results showed that suboptimal dietary quality negatively affected both growth and metamorphic development, and iodine supplementation only partially compensated for the effects on metamorphosis.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Cell Biology
Takashi Hasebe, Kenta Fujimoto, Atsuko Ishizuya-Oka
Summary: Recent research has shown that the Hippo signaling pathway plays an important role in intestinal remodeling during amphibian metamorphosis. This study found that the expression of genes involved in the Hippo pathway is regulated by thyroid hormone (TH) in the metamorphosing intestine of Xenopus laevis. The transcriptional co-activator YAP1, a major effector of the Hippo signaling, is expressed in adult stem cells and surrounding connective tissue cells. The interaction between YAP1 and its binding partner TEAD1 is inhibited by verteporfin (VP), and this inhibition leads to decreased stem cell number and proliferation, resulting in the failure of adult epithelial development.
CELL AND TISSUE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Muriel Rigolet, Nicolas Buisine, Marylou Scharwatt, Evelyne Duvernois-Berthet, Daniel R. Buchholz, Laurent M. Sachs
Summary: Thyroid hormones and glucocorticoids play a role in cell-cycle regulation through the joint regulation of genes in the liver of tadpoles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
Natalya Zahn, Christina James-Zorn, Virgilio G. Ponferrada, Dany S. Adams, Julia Grzymkowski, Daniel R. Buchholz, Nanette M. Nascone-Yoder, Marko Horb, Sally A. Moody, Peter D. Vize, Aaron M. Zorn
Summary: Normal tables and illustrations of embryonic development play a crucial role in research and teaching. However, due to copyright restrictions, the use of classic illustrations is no longer possible. To address this, we have created a new resource consisting of 133 high-quality illustrations and a landmarks table, which will support future Xenopus research and teaching.
Article
Anatomy & Morphology
Brian P. Heubel, Carson A. Bredesen, Thomas F. Schilling, Pierre Le Pabic
Summary: Background Endochondral ossification is a major bone forming mechanism in vertebrates, defects in which can result in skeletal dysplasia or craniofacial anomalies in humans. The zebrafish holds great potential to advance our understanding of endochondral growth zone development and genetics, yet several important aspects of its biology remain unexplored. Here we provide a comprehensive description of endochondral growth zones in the pharyngeal skeleton, including their developmental progression, cellular activity, and adult fates. Results Postembryonic growth of the pharyngeal skeleton is supported by endochondral growth zones located either at skeletal epiphyses or synchondroses.Col2a1aandcol10a1ain situ hybridization and anti-PCNA immunostaining identify resting-, hypertrophic- and proliferative zones, respectively, in pharyngeal synchondroses. Cellular hypertrophy and matrix deposition contribute little, if at all, to axial growth in most skeletal elements. Zebrafish endochondral growth zones develop during metamorphosis and arrest in adults. Conclusions Two endochondral growth zone configurations in the zebrafish pharyngeal skeleton produce either unidirectional (epiphyses) or bidirectional (synchondroses) growth. Cell proliferation drives endochondral growth and its modulation, in contrast to mammalian long bones in which bone length depends more on cell enlargement during hypertrophy and intramembranous ossification is the default mechanism of bone growth in zebrafish adults.
DEVELOPMENTAL DYNAMICS
(2021)
Review
Endocrinology & Metabolism
Yuta Tanizaki, Yuki Shibata, Wonho Na, Yun-Bo Shi
Summary: Amphibian metamorphosis, especially in anurans like Xenopus laevis and Xenopus tropicalis, is a valuable model to study how T3 regulates adult organ development due to its similarity to mammalian postembryonic development and its independence from maternal influence. Studies on intestinal remodeling have provided important insights into the molecular mechanisms of T3 regulation of cell fate during development, suggesting that T3-induced activation of the cell cycle program is crucial for larval epithelial cell death and the formation of adult intestinal stem cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Ecology
Karine Rousseau, Sylvie Dufour, Laurent M. M. Sachs
Summary: This review focuses on the metamorphoses and developmental transitions in vertebrates, highlighting the important roles played by neuroendocrine axes in regulating these life transitions. It also discusses the impact of global climate change and environmental factors on developmental processes.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Review
Anatomy & Morphology
Christopher S. Rose, Danny Murawinski, Virginia Horne
JOURNAL OF ANATOMY
(2015)
Article
Zoology
Christopher S. Rose
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY
(2014)
Article
Multidisciplinary Sciences
Christopher S. Rose
Summary: Cartilage plays a crucial role in the development and evolution of vertebrate body shapes, especially in amphibians. This study provides a cell-level description of the larval growth and postembryonic shape change of major cartilages in a metamorphosing amphibian. The results offer insights into hormone-mediated remodeling, cartilage regeneration, and intrinsic shape regulating mechanisms, and suggest new directions for investigating skeletal shape and metamorphic ontogenies.