Review
Chemistry, Multidisciplinary
Artem A. Sizikov, Marianna V. Kharlamova, Maxim P. Nikitin, Petr I. Nikitin, Eugene L. Kolychev
Summary: Magnetic nanoparticles are widely used in nanobiomedicine for diagnostics, treatments, and drug delivery. Their unique magnetic properties allow for targeted delivery to tumors or tissues when a magnetic field is applied. The combination of magnetic drug targeting and gene delivery, known as magnetofection, is a promising approach for efficient delivery of genetic material to cells using controlled external magnetic fields.
Review
Pharmacology & Pharmacy
Artem A. Sizikov, Petr I. Nikitin, Maxim P. Nikitin
Summary: Nanoparticle-based technologies are being rapidly developed in various fields including biomedicine and molecular science. Magnetic nanoparticles have unique abilities to respond to magnetic fields, making them attractive for in vivo applications such as magnetofection. However, data on in vivo magnetofection processes after systemic injection of magnetic nanostructures are often limited, poorly studied, and sometimes contradictory. Emphasis has been placed on analyzing the structure and coating of nanomagnetic vectors, as well as considering current challenges and future trends in method development.
Article
Chemistry, Analytical
Jennifer Lien, Tawni Bull, Richard W. Michelmore, Ting Guo
Summary: Fluorescence titration using magnetic nanoparticles (FTMN) is a rapid, inexpensive, and simple method for quantifying the amount of fluorophore-intercalated plasmid DNA on DNA attractive nanoparticles. By confirming the binding of propidium iodide (PI)-intercalated DNA to polyethylenimine (PEI)-coated monodisperse iron oxide magnetic nanoparticles (PEI-MNs), the FTMN method provides a way to determine the amount of DNA on MNs, the dissociation constant, and binding energy. This method is validated by utilizing quantitative polymerase chain reaction to measure the amount of free PI/DNA in solution.
ANALYTICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Luis Daniel Terrazas-Armendariz, Cynthia Aracely Alvizo-Baez, Itza Eloisa Luna-Cruz, Becky Annette Hernandez-Gonzalez, Ashanti Concepcion Uscanga-Palomeque, Mitchel Abraham Ruiz-Robles, Eduardo Gerardo Perez Tijerina, Cristina Rodriguez-Padilla, Reyes Tamez-Guerra, Juan Manuel Alcocer-Gonzalez
Summary: In this study, the Magnetogene nanoparticle vector was developed to increase selective gene expression in tumor cells and reduce toxicity in healthy cells by taking advantage of the hypoxic microenvironments of solid tumors. The vector was guided by a magnet to the tumor tissue, demonstrating its selectivity and efficiency in both in vitro and in vivo experiments.
Article
Multidisciplinary Sciences
Martin Donnelley, Patricia Cmielewski, Kaye Morgan, Juliette Delhove, Nicole Reyne, Alexandra McCarron, Nathan Rout-Pitt, Victoria Drysdale, Chantelle Carpentieri, Kathryn Spiers, Akihisa Takeuchi, Kentaro Uesugi, Naoto Yagi, David Parsons
Summary: By studying the behavior of lentiviral-magnetic particles in the trachea of rats, it was found that lentiviral-magnetic particles and magnetic fields can improve gene vector targeting and transduction levels in the conducting airways.
SCIENTIFIC REPORTS
(2022)
Article
Nanoscience & Nanotechnology
S. S. Rohiwal, T. D. Nguyen, E. Kamenna, J. Klima, M. Vaskovicova, D. Sekac, M. Slouf, E. Pavlova, P. Stepanek, D. Babuka, H. Benes, O. Pop-Georgievski, P. Ecorchard, P. Bezdicka, D. Smrzova, K. Stieger, Z. Ellederova
Summary: Huntington's disease (HD) is a genetic disorder that mainly affects neurons in the striatum due to the presence of a toxic protein. Nanoparticles (NPs) have been developed as a promising delivery system for therapeutic RNA in treating HD. In this study, magnetic NPs (MNPs) were generated and characterized, and they showed low cytotoxicity and high transfection efficiency in delivering interfering RNA into cells. The MNPs were able to downregulate the production of the toxic protein, suggesting their potential as a treatment strategy for HD.
ACS APPLIED NANO MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Viktor Maurer, Selin Altin, Didem Ag Seleci, Ajmal Zarinwall, Bilal Temel, Peter M. Vogt, Sarah Strauss, Frank Stahl, Thomas Scheper, Vesna Bucan, Georg Garnweitner
Summary: The use of non-ionic surfactant vesicles as a nanocarrier for LFG-specific siRNA successfully downregulated the LFG gene in breast cancer cells, leading to increased efficacy of chemotherapeutics. The application of an external magnetic field enhanced siRNA internalization and activation of apoptotic signaling pathways.
Review
Materials Science, Biomaterials
Behnam Azadpour, Nazli Aharipour, Amirhosein Paryab, Hamed Omid, Sorosh Abdollahi, Hamidreza Madaah Hosseini, Adrine Malek Khachatourian, Muhammet S. Toprak, Alexander M. Seifalian
Summary: Gene therapy involves replacing or adding genes to cure diseases or improve the body's ability to fight diseases. Recent studies have shown that using viral vectors and magnetic nanoparticles can increase gene transfection efficiency, leading to sustained and successful gene therapy.
BIOMATERIALS ADVANCES
(2023)
Article
Nanoscience & Nanotechnology
Ya Wang, Giovanni Boero, Xiaosheng Zhang, Juergen Brugger
Summary: Researchers have demonstrated a fully biodegradable composite membrane made from silk fibroin and magnetic nanoparticles. The membrane's porosity can be remotely modified using an alternating magnetic field, allowing for controlled drug release.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biophysics
Mhd Anas Tomeh, Roja Hadianamrei, Defeng Xu, Stephen Brown, Xiubo Zhao
Summary: This study developed a new method for producing peptide-functionalized magnetic silk nanoparticles for targeted delivery to cancer cells. The functionalized nanoparticles showed higher selectivity towards cancer cells, increased cellular uptake and improved anticancer activity of the drug ASC-J9.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biophysics
Chi-Hsien Liu, Cheng-Han Lin, Yi-Jun Chen, Wei-Chi Wu, Chun-Chao Wang
Summary: Nucleic acids have potential for treating diseases but face challenges in cellular uptake. This study developed a new carrier, MNP-CA-PEI, for efficient delivery of nucleic acids into cells, enhancing gene silencing efficiency. The nanoparticles could be magnetically triggered to deliver DNA plasmids and siRNA, improving gene knockdowns in different cell lines.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Rene Stein, Felix Pfister, Bernhard Friedrich, Pascal-Raphael Blersch, Harald Unterweger, Anton Arkhypov, Andriy Mokhir, Mikhail Kolot, Christoph Alexiou, Rainer Tietze
Summary: Nanoformulations for delivering nucleotides into cells have gained attention for vaccinations and treatment. This study focuses on synthesizing superparamagnetic iron oxide nanoparticles for potential magnetofection applications. The functionalized nanoparticles showed improved transfection efficiency and stability, making them a promising candidate for magnetofection agents.
Article
Chemistry, Multidisciplinary
Liang Liu, Mengying Wang, Chaobing Liu, Zhaojun Yang, Xin Chen
Summary: This study developed ternary nanoparticles by coating silver nanoparticles with a polymeric layer of dopamine and grafting polyethyleneimine onto the surface. The nanoparticles showed good stability, low cytotoxicity, and high transfection efficiency, indicating their potential as safe and efficient gene vectors.
JOURNAL OF NANOPARTICLE RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Nicole B. Day, William C. Wixson, C. Wyatt Shields
Summary: Immunotherapy is a rapidly developing area in cancer treatment, with magnetic systems offering advantages in improving performance by increasing spatiotemporal control, reducing off-target effects, and enhancing efficacy. These systems enable various methods for programming immune responses, including magnetic hyperthermia, targeted delivery of drug carriers, structual changes in biomaterials driven by magnets, and interactions with cellular receptors using magnetic particles for promoting antitumor activity.
ACTA PHARMACEUTICA SINICA B
(2021)
Review
Chemistry, Multidisciplinary
Jens Casper, Susanne H. Schenk, Elahehnaz Parhizkar, Pascal Detampel, Ali Dehshahri, Jorg Huwyler
Summary: This review summarizes the applications of Polyethlyenimine (PEI) in DNA delivery, discusses the obstacles to its clinical use, and highlights potential future prospects. The review is divided into three sections, covering the mechanism of action, cellular toxicity, in vivo performance, and clinical trials of PEI-based formulations.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Physical
Roja Hadianamrei, Mhd Anas Tomeh, Stephen Brown, Jiqian Wang, Xiubo Zhao
Summary: The study introduced a new library of short cationic amphiphilic α-helical anticancer peptides with selective cytotoxicity against colorectal and cervical cancer, and investigated their anticancer mechanisms through a series of experiments.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Materials Science, Biomaterials
Ting Dai, Jiayi Ma, Su Ni, Chun Liu, Yan Wang, Siyu Wu, Jun Liu, Yiping Weng, Dong Zhou, Ana Jimenez-Franco, Hongbin Zhao, Xiubo Zhao
Summary: Electrospun PCL scaffolds, widely used in tissue engineering, have the potential to mimic the natural extracellular matrix structure. However, their small pore size and low bioactivity hinder cell migration and tissue formation. In this study, different materials were used to fabricate porous scaffolds, and the removal of water-soluble fibers increased the porosity. Biocompatibility and osteogenesis were evaluated, and the results showed that washed PCL/PEG/ATP scaffolds promoted better cell infiltration and differentiation into osteoblasts compared to PCL scaffolds. In vivo studies also demonstrated enhanced bone regeneration in rat cranium defects using ATP-doped electrospun PCL scaffolds.
BIOMATERIALS ADVANCES
(2022)
Article
Pharmacology & Pharmacy
Ruicheng Xu, Mhd Anas Tomeh, Siyuan Ye, Peng Zhang, Songwei Lv, Rongrong You, Nan Wang, Xiubo Zhao
Summary: The study demonstrated the use of microfluidic swirl mixers for large-scale production of liposomes with high productivity and better size control. These liposomes effectively delivered curcumin into cancer cells, showing promising potential for drug delivery systems.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Engineering, Biomedical
Wen Qin, Chenkai Li, Chun Liu, Siyu Wu, Jun Liu, Jiayi Ma, Wenyang Chen, Hongbin Zhao, Xiubo Zhao
Summary: In this study, 3D printed GO/ATP/COL composite scaffolds were developed. The composite scaffolds showed excellent printability and mechanical strength. In vitro and in vivo experiments demonstrated that the composite scaffolds exhibited good biocompatibility and osteogenic ability, promoting bone tissue regeneration.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2022)
Article
Chemistry, Analytical
Songwei Lv, Dong Zheng, Zhaoxian Chen, Bin Jia, Peng Zhang, Jiaxuan Yan, Xiubo Zhao, Wanlan Jiang, Jing-Juan Xu
Summary: This study designed a near-infrared light-responsive microfluidic chip for biocompatible single-cell manipulation and study of CTC heterogeneity. The chip combined immunomagnetic labeling and a gradient magnetic field to distribute CTCs in different regions. The lateral flow microarray chip achieved high capture efficiency and purity, and the captured single CTC cell can be released individually by near-infrared at a specific point.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Xinyu Xu, Yujie Ding, Roja Hadianamrei, Songwei Lv, Rongrong You, Fang Pan, Peng Zhang, Nan Wang, Xiubo Zhao
Summary: This study demonstrates the potential application of functionalized gold nanorods with antimicrobial peptides in photothermal therapy for bacterial infections. The conjugation of peptides increased selectivity, antimicrobial activity, and biocompatibility of the gold nanorods. The experiments conducted in vitro and in a mouse model of wound infection highlight the effective antimicrobial effects and wound healing properties of the peptide-functionalized gold nanorods with and without laser irradiation.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biophysics
Fang Pan, Yueping Li, Yujie Ding, Songwei Lv, Rongrong You, Roja Hadianamrei, Mhd Anas Tomeh, Xiubo Zhao
Summary: This study systematically designed a series of new alpha-helical short peptides with different anticancer activities and demonstrated their interaction mechanism with cancer cells. The peptides exhibited high selectivity towards cancer cells due to their balanced amphiphilicity and cationic nature, while showing low toxicity to normal cells. Furthermore, the peptides not only damaged the mitochondria and induced apoptosis in cancer cells, but also activated the Caspase enzyme-linked reaction. The results suggest the potential of these peptides for clinical applications and provide guidance for the development of highly selective anticancer medications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Yi Jin, Mhd Anas Tomeh, Peng Zhang, Mingzhu Su, Xiubo Zhao, Zhiqiang Cai
Summary: This study utilized a newly developed microfluidic swirl mixer to fabricate temperature-sensitive liposomes (TSL) for the treatment of breast cancer through near-infrared stimulation. The results demonstrated that AP-3/ICG@TSL significantly inhibited the growth of breast cancer both in vitro and in vivo without causing damage to other organs. These findings suggest that the AP-3/ICG@TSL manufactured using the microfluidic swirl mixer is a promising drug delivery system for breast cancer therapy.
Review
Materials Science, Multidisciplinary
Nan Wang, Xiubo Zhao
Summary: This article systematically introduces the important role of atomic force microscopy (AFM) in studying contact electrification (CE) phenomena, especially in researching the CE mechanism at the nanoscale. In addition, the working mechanism of metal-semiconductor direct current triboelectric nanogenerators based on conductive atomic force microscopy (C-AFM) studies is also introduced. The potential utility and limitations of AFM in future TENG technology development and its improvement direction are analyzed. This review provides an updated overview and understanding of the existing challenges and opportunities, and it is hoped to contribute to the advancement of TENG technologies.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Engineering, Biomedical
David Alexander Gregory, Annabelle T. R. Fricker, Peter Mitrev, Meghna Ray, Emmanuel Asare, Daniel Sim, Soponvit Larpnimitchai, Zixuan Zhang, Jinge Ma, Santosh S. V. Tetali, Ipsita Roy
Summary: In recent decades, Additive Manufacturing has made great advancements and has become increasingly important in biomedical applications. This study focuses on various biomedical devices and proposes CAD designs for bone implants, tooth implants, tissue repair patches, nerve guidance conduits, and coronary artery stents. The study demonstrates the ability to create patient-specific implants using real CT scans and the use of a blend of biocompatible and bioresorbable natural polymers for fabrication. The findings suggest that FDM is useful for a wide range of soft tissue applications, but more elastomeric materials may be needed.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Roja Hadianamrei, Mhd Anas Tomeh, Jiqian Wang, Stephen Brown, Xiubo Zhao
Summary: In this study, two new surfactant-like peptides (SLPs), named (IA)4K and (IG)4K, were developed for selective delivery of antisense oligodeoxynucleotides (ODNs) and small interfering RNA (siRNA) to cancer cells. The interaction of the peptides with nucleic acids, their transfection efficiency, and cytotoxicity were investigated. Both SLPs showed high transfection efficiency and selectivity for cancer cells, and exhibited low cytotoxicity. This study provides insights into the structural features required for nucleic acid complexation and delivery, and serves as a guide for the design of new SLPs for selective gene delivery to minimize adverse effects in healthy tissues.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Materials Science, Biomaterials
Yu Xia, Ruicheng Xu, Siyuan Ye, Jiaxuan Yan, Piyush Kumar, Peng Zhang, Xiubo Zhao
Summary: Current anticancer research suggests that combining multiple treatment methods can enhance the killing of tumor cells. In this study, we developed multi-responsive targeted antitumor nanoparticles (NPs) using microfluidic swirl mixer technology. The NPs were made of folate-functionalized gelatin NPs, CuS NPs, Fe3O4 NPs, and curcumin. The results showed that the combination of photothermal-ablation therapy, chemotherapy, and targeted delivery of the NPs efficiently killed tumor cells.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biophysics
Piyush Kumar, Ana Jimenez Franco, Xiubo Zhao
Summary: 3D cell culture is a technique that mimics in vivo environment for cells to grow in vitro. This study proposes two independent 3D cell culture models for different applications. One uses porous spherical structures as carriers to retain cell shape, while the other uses 3D printed silk fibroin structures for directed cell growth. These models have advantages in cell research, such as drug discovery and treatment of neuropathies.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Physical
Jinguo Qiu, Mhd Anas Tomeh, Yi Jin, Bo Zhang, Xiubo Zhao
Summary: This study presented a high-performance microfluidic device to fabricate ACP loaded nanoparticles (NPs) with controllable properties. The encapsulated ACP showed reduced toxicity, controlled release, and increased cell uptake, leading to enhanced anticancer effects. The development of At3 loaded NPs provides a promising approach for ACP-based cancer therapy.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Materials Science, Biomaterials
Songwei Lv, Jingfang Wang, Rongrong You, Suyu Liu, Yujie Ding, Roja Hadianamrei, Mhd Anas Tomeh, Fang Pan, Zhiqiang Cai, Xiubo Zhao
Summary: This study investigated the relationship between the chemical structure and biological activity of AMPs derived from a naturally occurring AMP from ants. The designed peptides showed enhanced antibacterial activity by replacing cationic amino acids with hydrophobic amino acids in the hydrophobic region, and improved selectivity for bacteria by increasing the number of cationic amino acids in the hydrophilic region. Four of the designed peptides displayed significant antibacterial activity and high selectivity for bacteria. The results can provide a better understanding of the structural features required for strong antibacterial activity and selectivity, and guide the future rational design of AMPs.
BIOMATERIALS SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
