Article
Agriculture, Multidisciplinary
Yixin An, Ziqi Wang, Baijian Liu, Yuwei Cao, Lin Chen
Summary: This study investigates the translation regulation of plant genes under salt stress and finds that salt stress inhibits gene translation efficiency. It also identifies various small ORFs in the genome of Medicago truncatula, which play a role in the translation regulation of genes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Yixin An, Haotian Su, Qichen Niu, Shuxia Yin
Summary: Salt stress response in Medicago truncatula involves differentially expressed coding and non-coding RNA, including mRNAs, lncRNAs, circRNAs, and miRNAs, which function in various pathways. The interactions between different RNAs were predicted based on the ceRNA theory, revealing insights into salt stress response in leguminous plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yichen Kang, Xinyu Yang, Yuhui Liu, Mingfu Shi, Weina Zhang, Yanling Fan, YanHong Yao, Junlian Zhang, Shuhao Qin
Summary: This study characterized the morphological, physiological, and transcriptome changes of tissue culture seedlings of potato variety under alkali stress, identifying key pathways and specific miRNAs and mRNAs involved in the response. The results provide new insights into the molecular mechanisms of potato's response to alkali stress, offering potential candidates for molecular-assisted screening and salt-alkali resistance breeding.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Plant Sciences
Shuwei Dong, Lijun Sang, Hongli Xie, Maofeng Chai, Zeng-Yu Wang
Summary: This study investigated the impact of salt stress on leaf senescence in Medicago truncatula, identifying numerous senescence-associated genes (SAGs) at different time points and linking them to processes such as protein metabolism, photosynthesis, chlorophyll metabolism, and hormone signaling. Additionally, various transcription factor families, including NAC, bHLH, MYB, and ERF, were found to be associated with salt stress-induced leaf senescence, providing new insights for further genetic research and breeding in forage crops.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Lei Xu, Lixia Zhang, Yajiao Liu, Bilig Sod, Mingna Li, Tianhui Yang, Ting Gao, Qingchuan Yang, Ruicai Long
Summary: This study identified EF genes in Medicago truncatula and investigated the salt stress response function of MtEF1A1. The results showed that overexpression of MtEF1A1 enhanced plant resistance to salt stress and reduced oxidative stress levels. Additionally, the expression levels of stress-responsive and calcium-binding genes were upregulated in MtEF1A1-overexpressing M. truncatula.
Article
Plant Sciences
Rui Tian, Xiaohan Sun, Cuimei Liu, Jinfang Chu, Mingui Zhao, Wen-Hao Zhang
Summary: Increasing evidence suggests that lncRNAs play a role in regulating plant tolerance to abiotic stress. This study characterized a salt stress-responsive lncRNA, MtCIR1, from M. truncatula and found that it negatively regulates salt stress response by affecting ABA metabolism and signaling during seed germination and Na+ transport under salt stress during seedling growth in Arabidopsis thaliana. These findings advance our understanding of the regulatory roles of lncRNAs in plant response to salt stress.
Article
Biochemistry & Molecular Biology
Yaying Xia, Junfeng Yang, Lin Ma, Su Yan, Yongzhen Pang
Summary: The study identified 346 cytochrome P450 genes and found multiple hub genes involved in plant growth and development under drought/salt stresses. These hub genes were induced by the stresses and mainly involved in secondary metabolism and fatty acid pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Forestry
Zhiquan Wang, Fengjiao Zhang, Qin Shi, Rui Zhang, Yunlong Yin, Chaoguang Yu
Summary: This study aimed to discover the miRNAs responding to salt stress. Small RNA sequencing was performed in the salt-tolerant Taxodium hybrid 'Zhongshanshan 405' and 52 differentially expressed miRNAs were found. Enrichment analysis of target genes showed that miRNAs may play key roles in regulating the tolerance to salt stress in 'Zhongshanshan 405'. The study also identified two important genes regulated by miRNAs under salt treatment, which can be selected for future research.
Article
Agronomy
Shuwen Li, Hong Xie, Yinglong Chen, Wei Jia, Di Dong, Mengdi Wang, Yinruizhi Li, Tiejun Zhang, Liebao Han, Yuehui Chao
Summary: This study cloned a gene called MtTGA1 from Medicago truncatula and found that it was highly expressed in the root. The expression of MtTGA1 was upregulated after SA and BR treatments, but downregulated after GA and ABA treatments. Transgenic plants with MtTGA1 showed longer root lengths and increased activity of antioxidant enzymes. MtTGA1 also interacted with SPX1 protein. These findings are important for understanding the regulatory network of plant growth and development mediated by the TGA1 transcription factor family.
Article
Plant Sciences
Zhengmei Yang, Tingting Dong, Xibin Dai, Yiliang Wei, Yujie Fang, Lei Zhang, Mingku Zhu, Ghazala Nawaz, Qinghe Cao, Tao Xu
Summary: This study identified and compared miRNAs in sweetpotato cultivars with different salt sensitivity, and found differentially expressed miRNAs and salt-responsive miRNAs. The target genes of these miRNAs were mainly transcription factors and proteins related to metabolism and stress response. These findings contribute to understanding the regulatory pattern of miRNAs in different sweetpotato genotypes and provide references for improving the agronomic traits of sweetpotato through miRNA manipulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Medicine, General & Internal
Xuejun He, Ningzhi Zhang, Wenye Cao, Yiqiao Xing, Ning Yang
Summary: Advancements in high-throughput sequencing (HTS) technology have provided new insights into the etiology, signaling pathways, and biomarkers of ocular diseases, aiding in improving their diagnosis and research.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Plant Sciences
Qihui Gui, Zhengyu Yang, Chao Chen, Feng Yang, Song Wang, Rui Dong
Summary: This study revealed the important regulatory role of lncRNAs in the response to aluminum stress in the legume model plant Medicago truncatula. The lncRNAs were found to be involved in regulating plant hormone signal transduction, cell wall modification, and the tricarboxylic acid cycle. Additionally, two lncRNAs associated with aluminum-activated malate transporter were shown to enhance aluminum stress tolerance. These findings enhance our understanding of the role of lncRNAs in the response to aluminum stress in legumes and provide potential lncRNA candidates for further studies.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Huan Du, Zhicheng Jiao, Junjie Liu, Wei Huang, Liangfa Ge
Summary: A new strategy using deep-coverage whole-genome sequencing, polymorphism calling, and customized filtering was developed to sensitively identify mutation sites for fast neutron bombardment (FNB) mutants. This method demonstrated robustness in identifying causative genes for white nodule and yellow leaves mutants in Medicago truncatula, showing potential for efficient mutation site identification in FNB mutants.
Article
Plant Sciences
Maowei Guo, Hongyan Li, Lin Zhu, Zinian Wu, Jun Li, Zhiyong Li
Summary: The study explored genes conferring drought resistance to Medicago ruthenica through analyzing miRNAs and their potential target genes under osmotic stress conditions. The study identified 591 miRNAs and predicted 11 target genes, showing that miR319-TCP4 module may act as a homeostasis factor in M. ruthenica roots following drought injury. This module is conserved among plant species.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Genetics & Heredity
Jiefei Nai, Tieming Ma, Yingjie Liu, Yunwei Zhou
Summary: In this study, high-throughput sequencing was used to identify known and new miRNAs in the Chrysanthemum variety Niu 9717. The miRNAs were found to form miRNA-target pairs with mRNAs in the root and leaf, and were involved in pathways related to hormone signaling during plant growth and development.
Article
Multidisciplinary Sciences
Ruicai Long, Yanli Gao, Hao Sun, Tiejun Zhang, Xiao Li, Mingna Li, Yan Sun, Junmei Kang, Zhen Wang, Wang Ding, Qingchuan Yang
SCIENTIFIC REPORTS
(2018)
Article
Plant Sciences
Ruicai Long, Hao Sun, Chunyu Cao, Tiejun Zhang, Junmei Kang, Zhen Wang, Mingna Li, Yanli Gao, Xiao Li, Qingchuan Yang
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2019)
Article
Biochemical Research Methods
Yanli Gao, Ruicai Long, Junmei Rang, Zhen Wang, Tiejun Zhang, Hao Sun, Xiao Li, Qingchuan Yang
JOURNAL OF PROTEOME RESEARCH
(2019)
Article
Biochemical Research Methods
Hao Sun, Ruicai Long, Fan Zhang, Tiejun Zhang, Junmei Kang, Zhen Wang, Chunyu Cao, Jie Yu, Qingchuan Yang
PLANT MOLECULAR BIOLOGY REPORTER
(2019)
Article
Biochemistry & Molecular Biology
Jie Yu, Hao Sun, Jiaju Zhang, Yiyao Hou, Tiejun Zhang, Junmei Kang, Zhen Wang, Qingchuan Yang, Ruicai Long
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Hao Sun, Jie Yu, Fan Zhang, Junmei Kang, Mingna Li, Zhen Wang, Wenwen Liu, Jiaju Zhang, Qingchuan Yang, Ruicai Long
Article
Biochemistry & Molecular Biology
Hao Sun, Huiting Cui, Jiaju Zhang, Junmei Kang, Zhen Wang, Mingna Li, Fengyan Yi, Qingchuan Yang, Ruicai Long
Summary: The study identified mutant lines with a dwarf phenotype due to Tnt1 retrotransposon insertion in the gibberellin 3-beta-dioxygenase 1 gene (GA3ox1), inhibiting the synthesis of bioactive gibberellic acids (GAs). Analysis revealed shorter plant height, root, and petiole length in mutants compared to the wild type, with smaller leaf size attributed to cell-size diminution. Proteomic and metabolomic analyses indicated up-regulation of flavonoid isoflavonoid biosynthesis and down-regulation of nitrogen metabolism in the mutants, suggesting a critical role of GAs in regulating nitrogen metabolism positively and flavonoid biosynthesis negatively in leaves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)