Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 53, Issue 4, Pages 435-441Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2018.12.006
Keywords
ESBL-E; Metagenomics; Intestinal microbiota; Antibiotic resistance
Funding
- Thailand Research Fund through the Royal Golden Jubilee PhD Program [PHD/0072/2557]
- Siriraj Research Fund [(IO) R015833012]
- Faculty of Medicine Siriraj Hospital, Mahidol University
- Graduate Partnership Program of the U.S. National Institutes of Health (NIH)
- Intramural Research Program of the National Institute of Allergy and Infectious Diseases (NIAID), NIH [ZIA AI00 0904]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [ZICAI001233, ZIAAI000904] Funding Source: NIH RePORTER
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There is increasing recognition that the intestinal microbiota govern human well-being and prevent diseases. Intestinal colonization by antibiotic-resistant pathogens, however, can lead to the spread of resistance as well as serious infections. Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) represent particularly dangerous pathogens, which are known to asymptomatically colonize the intestinal tract in the community. Here, we performed a 16S rRNA metagenomics sequence analysis to analyse differences in the microbiota composition between ESBL-E carriers and non-carriers in Thailand, where ESBL-E carriage rates are notoriously high. The most notable difference detected was that the phylum Bacteroidetes, and in particular, the species Bacteroides uniformis, were significantly more abundant in ESBL-E non-carriers than carriers. The Shannon diversity index in non-carriers (5.10 +/- 0.69) was also lower than that in ESBL-E carriers (5.39 +/- 0.48) without statistical significance (P = 0.13). The overall beta diversity difference of the intestinal microbiota of ESBL-E carriers as compared to non-carriers was statistically significant (Adonis on weighted unifrac: R-2 = 0.14, P = 0.005). Furthermore, ESBL-E carriage was significantly lower in farmers than in those with other occupations. Our findings suggest that a dynamic interaction exists between microbiota diversity and ESBL-E carriage, which is possibly driven by dietary composition and may be exploited using probiotic approaches to control the spread of ESBL-E. (C) 2018 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
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