Article
Biochemistry & Molecular Biology
Mohtadin Hashemi, Siddhartha Banerjee, Yuri L. Lyubchenko
Summary: The effects of membranes on the early-stage aggregation of amyloid beta (A beta) have been studied, showing that direct A beta-membrane interactions dramatically enhance the aggregation process. Cholesterol in membranes significantly enhances the aggregation kinetics and accelerates the formation and dissociation of aggregates. Cholesterol binds A beta monomers and changes their conformation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Andressa R. Pereira, Flavio M. Shimizu, Osvaldo N. Oliveira Jr
Summary: The composition of Langmuir monolayers, specifically the relative concentration of cholesterol, plays a crucial role in modulating the effects of the antineoplastic drug paclitaxel. The dependence on cholesterol concentration for these monolayers simulating lipid rafts is non-monotonic, affecting surface pressure and compressibility modulus differently. The physiological action of paclitaxel may vary depending on its interaction with membrane penetration or membrane elasticity changes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Multidisciplinary Sciences
Carmen N. Hernandez-Candia, Sarah Pearce, Chandra L. Tucker
Summary: The DisCo method allows for fast, inducible, and specific disruption of tagged condensates in mammalian cells by using chemical dimerizers to induce the recruitment of a ligand into condensates leading to condensate disassembly.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Minggang Tian, Yaru Sun, Xiuqi Kong, Baoli Dong
Summary: Studying the phase separation of ER membranes is crucial for understanding the fundamental biology of ER. However, NIR ratiometric imaging of ER phase behaviors has not been explored in living cells and diverse tissues. In this study, we developed a polarity-responsive NIR fluorescent probe to visualize the phase behavior in ER membranes and revealed different phase behaviors in different tissues.
ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Dingkang Liu, Haibo Rong, Ye Chen, Qun Wang, Sijia Qian, Yue Ji, Wenbing Yao, Jun Yin, Xiangdong Gao
Summary: The engineered therapeutic protein PAK has been found to reverse multidrug resistance in cancer cells, enhancing their sensitivity to chemotherapy. PAK induces mitochondrial injury by selectively inhibiting mitochondrial complex V, leading to redistribution of ABC transporters and reversal of drug resistance.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biophysics
Martin Girard, Tristan Bereau
Summary: The plasma membrane of eukaryotes exhibits lipid asymmetry, with different lipids primarily located in different leaflets. Using computational simulations, it has been found that saturation asymmetry arises as a result of phospholipid number asymmetry and sphingomyelin contents. This suggests that some asymmetries in lipid composition may naturally result from others and do not require external factors. The asymmetry of cholesterol, on the other hand, is fairly mild and influenced by all the other asymmetries.
BIOPHYSICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Kevin N. Baumann, Greta Sneideriene, Michele Sanguanini, Matthias Schneider, Oded Rimon, Alicia Gonzalez Diaz, Heather Greer, Dev Thacker, Sara Linse, Tuomas P. J. Knowles, Michele Vendruscolo
Summary: Intracellular Afl42 is primarily produced in the endoplasmic reticulum and Golgi apparatus, where lipid bilayers have been shown to promote the aggregation of Afl. The study demonstrates that the ER and Golgi model membranes inhibit the aggregation of Afl42, suggesting an evolutionary optimization of lipid composition to prevent intracellular Afl aggregation.
ACS CHEMICAL NEUROSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Hannah S. Martin, Kira A. Podolsky, Neal K. Devaraj
Summary: Nucleotides, amino acids, sugars, and lipids are predominantly homochiral within individual cells on Earth. While oligonucleotides and proteins exhibit a single natural chirality, two stereoisomers of phospholipids have emerged in archaea and bacteria, leading to an evolutionary divergence known as the lipid divide. Research focuses on the emergence of phospholipid homochirality and compares the stability of synthetic homochiral and heterochiral membranes in vitro.
Article
Chemistry, Multidisciplinary
Luke E. Brennan, Lokesh K. Kumawat, Magdalena E. Piatek, Airlie J. Kinross, Daniel A. Mcnaughton, Luke Marchetti, Conor Geraghty, Conor Wynne, Hua Tong, Oisin N. Kavanagh, Finbarr O'Sullivan, Chris S. Hawes, Philip A. Gale, Kevin Kavanagh, Robert B. P. Elmes
Summary: This study reports the synthesis and structural characterization of a new class of fluorescent anionophores that effectively kill Gram-positive bacteria by disrupting normal Na+ and Cl- concentrations. The most active transporter shows potent inhibitory activity against Staphylococcus aureus (SA) and methicillin-resistant Staphylococcus aureus (MRSA). Cell-based assays and label-free quantitative proteomic profiling suggest that the mode of action is directly related to the anion-transport ability.
Article
Chemistry, Physical
Ricki Chairil, Noah Malmstadt
Summary: Curvature-mediated lipid-protein interactions play a crucial role in cellular reactions and mechanisms. This study investigates the membrane curvature partitioning behavior of cube-shaped CsPbBr3 quantum dots (QDs) embedded within deformed giant unilamellar vesicles (GUVs). The results show that the distribution of CsPbBr3 QDs differs significantly from that of conventional fluorophores and quasispherical CdSe core/ZnS shell QDs, suggesting that the geometry of both QDs and lipid membranes affect the curvature preferences of QDs. These findings provide insights into the aggregation of proteins induced by curvature and the interactions between lipid membranes and intercalating particles.
Article
Gastroenterology & Hepatology
Oishika Das, Aaheli Masid, Mainak Chakraborty, Animesh Gope, Shanta Dutta, Moumita Bhaumik
Summary: This study found that butyrate treatment reduces membrane cholesterol and disrupts lipid rafts, leading to a decrease in enteric bacterial invasion in cell. The study also demonstrated that butyrate treatment provides protection against pathogen invasion and reduces inflammation in mice.
Article
Food Science & Technology
Chontida Tangsongcharoen, Jose L. Toca-Herrera, Boonhiang Promdonkoy, Sudarat Tharad
Summary: This study investigated the importance of the conserved T144 residue in the alpha D-beta 4 loop of Cyt2Aa2 protein for lipid binding on fluid lipid membranes. The results showed that replacing threonine with alanine hinders the binding of Cyt2Aa2 on liquid lipid membranes, providing a possibility to modify the protein to specific cells via lipid phase selection.
Review
Agriculture, Multidisciplinary
Fan Zhang, Junhua Li, Cuihua Chang, Luping Gu, Wen Xiong, Yujie Su, Yanjun Yang
Summary: This review discusses the contradictory conclusions about the relationship between egg consumption and cardiovascular disease (CVD) risk, as well as the effects of egg cholesterol intake on cholesterol homeostasis. The study finds that moderate egg consumption has no substantial overall impact on cholesterol homeostasis in healthy individuals. However, excessive cholesterol intake is not recommended for individuals with metabolic disorders. In the future, the impact of egg consumption on CVD risk should be considered from different perspectives.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Polymer Science
Yuhang Song, Yu Geng, Lei Shen
Summary: This study provides new insights into the behavior of Aβ chains along important biological nanowire structures and highlights the significance of cellular shapes in manipulating peptide amyloidosis.
Article
Biochemistry & Molecular Biology
Yu-Shiuan Cheng, Tianyi Zhang, Xiang Ma, Sarida Pratuangtham, Grace C. Zhang, Alexander A. Ondrus, Amirhossein Mafi, Brett Lomenick, Jeffrey J. Jones, Alison E. Ondrus
Summary: Oxysterols, hydroxylated cholesterol metabolites, have important roles in health and disease. The chemoproteomics probe for 20(S)-hydroxycholesterol identified specific interactions with proteins involved in immune response and cancer. 20(S)-OHC was found to be a highly selective ligand for the protein transmembrane protein 97, providing insights into its biological activity and potential therapeutic targets.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Elyse S. Fischer, Warren A. Campbell, Shihui Liu, Rodolfo Ghirlando, Rasem J. Fattah, Thomas H. Bugge, Stephen H. Leppla
Article
Neurosciences
Warren A. Campbell, Ameya Deshmukh, Sydney Blum, Levi Todd, Ninoshka Mendonca, Jessica Weist, Joshua Zent, Thanh V. Hoang, Seth Blackshaw, Jennifer Leight, Andy J. Fischer
EXPERIMENTAL NEUROLOGY
(2019)
Article
Multidisciplinary Sciences
Thanh Hoang, Jie Wang, Patrick Boyd, Fang Wang, Clayton Santiago, Lizhi Jiang, Sooyeon Yoo, Manuela Lahne, Levi J. Todd, Meng Jia, Cristian Saez, Casey Keuthan, Isabella Palazzo, Natalie Squires, Warren A. Campbell, Fatemeh Rajaii, Trisha Parayil, Vickie Trinh, Dong Won Kim, Guohua Wang, Leah J. Campbell, John Ash, Andy J. Fischer, David R. Hyde, Jiang Qian, Seth Blackshaw
Article
Neurosciences
Warren A. Campbell, Amanda Fritsch-Kelleher, Isabella Palazzo, Thanh Hoang, Seth Blackshaw, Andy J. Fischer
Summary: Studies show that MDK expression in the retina is regulated by Muller glia, leading to effects on neuronal survival and the formation of MGPCs. MDK plays a crucial role in mediating glial activity and promoting the reprogramming of Muller glia into proliferating MGPCs.
Article
Neurosciences
Warren A. Campbell, Sydney Blum, Alana Reske, Thanh Hoang, Seth Blackshaw, Andy J. Fischer
Summary: The study investigated the role of endocannabinoids in the transition of Muller glia cells and the formation of retinal cells, finding that endocannabinoids influence the reactivity of MG and the formation of MGPCs but do not affect immune cells in the retina.
Article
Developmental Biology
Warren A. Campbell, Allen Tangeman, Heithem M. El-Hodiri, Evan C. Hawthorn, Maddie Hathoot, Sydney Blum, Thanh Hoang, Seth Blackshaw, Andy J. Fischer
Summary: A recent study on chick retinas found that fatty acid binding proteins (FABPs) are highly expressed and play a role in regulating the function of glial cells and microglia. Inhibition of FABPs leads to transitions of glial cells to reactive states and suppresses the formation of progenitor cells, while inhibition of fatty acid synthase (FASN) increases microglia proliferation and suppresses progenitor cell formation.
Article
Neurosciences
Heithem M. El-Hodiri, Warren A. Campbell, Lisa E. Kelly, Evan C. Hawthorn, Maura Schwartz, Archana Jalligampala, Maureen A. McCall, Kathrin Meyer, Andy J. Fischer
Summary: The regenerative potential of Muller glia varies across species, with fish showing extraordinary potential and mammals showing limited potential. NFIs are identified as cell-intrinsic factors that suppress the neurogenic potential of MGPCs in the chick retina, and their expression patterns in developing and mature retinas have been characterized.
JOURNAL OF COMPARATIVE NEUROLOGY
(2022)
Article
Neurosciences
Warren A. Campbell, Heithem M. El-Hodiri, Diego Torres, Evan C. Hawthorn, Lisa E. Kelly, Leo Volkov, David Akanonu, Andy J. Fischer
Summary: Chromatin access and epigenetic gene silencing play important roles in regulating gene expression during developmental processes. This study investigates the expression and functions of S-adenosylhomocysteine hydrolase (SAHH) and histone methyltransferases (HMTs) in the formation of Muller glia-derived progenitor cells (MGPCs) in chick and mouse retinas. In chick, inhibition of SAHH blocks the formation of MGPCs by reducing H3K27me3 levels, while in the mouse retina, SAHH inhibition has no effect on neuron-like cell differentiation. These findings suggest that SAHH and HMTs are required for the reprogramming of MG into MGPCs in chick retinas.
Article
Chemistry, Multidisciplinary
Maxmore Chaibva, Xiang Gao, Pranav Jain, Warren A. Campbell, Shelli L. Frey, Justin Legleiter