Article
Reproductive Biology
F. Magata, M. Urakawa, F. Matsuda, Y. Oono
Summary: The use of sexed semen to produce bovine embryos results in embryos with slower growth, reduced cell numbers, and decreased viability compared to embryos produced with unsorted semen. These differences may be attributed to abnormalities during the first cleavage and could explain the reduced fertility observed in embryos derived from sexed semen.
Article
Cell Biology
Amy K. Webster, Rojin Chitrakar, Seth M. Taylor, L. Ryan Baugh
Summary: This study investigates the gene expression changes and regulatory strategies in the somatic and germline cells of C. elegans under starvation. The results show that relative expression of germline-enriched genes increases during starvation, while somatic transcription is required for survival. This work reveals the importance of transcriptional regulation during starvation and the different strategies employed by somatic and germline cells.
Article
Genetics & Heredity
Pierre Gonczy, Fernando R. Balestra
Summary: While both sperm and egg contribute nuclear genetic material to the zygote in metazoan organisms, the distribution of other cellular constituents, particularly centrioles, is unequal between the two gametes. This study investigated the distribution of sperm-contributed centrioles in Caenorhabditis elegans embryos and found that their distribution is stochastic. These findings suggest that, unlike in some asymmetric cell divisions, centrioles contributed by the male germ line are randomly distributed in C. elegans embryos.
Article
Obstetrics & Gynecology
A. Cardona Barberan, D. Bonte, A. Boel, V Thys, R. Paredis, F. Machtelinckx, P. De Sutter, I De Croo, L. Leybaert, D. Stoop, P. Coucke, F. Vanden Meerschaut, B. Heindryckx
Summary: The study aimed to investigate whether assisted oocyte activation (AOA) could overcome recurrent embryo developmental problems after ICSI. The results showed that AOA did not improve blastocyst formation in patients with recurrent embryo developmental problems after ICSI.
HUMAN REPRODUCTION
(2023)
Article
Ecology
Phillip L. Davidson, Haobing Guo, Jane S. Swart, Abdull J. Massri, Allison Edgar, Lingyu Wang, Alejandro Berrio, Hannah R. Devens, Demian Koop, Paula Cisternas, He Zhang, Yaolei Zhang, Maria Byrne, Guangyi Fan, Gregory A. Wray
Summary: Changes in developmental gene regulatory networks contribute to the diversity of life, and the evolutionary mechanisms behind these regulatory interactions are still not clear. By analyzing the genomes, epigenomes, and transcriptomes of sea urchin species, researchers found positive selection and chromatin accessibility modifications in putative regulatory elements on the branch leading to the derived life history. Single-cell transcriptomes revealed delayed cell fate specification and fewer open chromatin regions in the derived state. Experimentally perturbing key transcription factors disrupted conserved regulatory interactions in early embryonic patterning events. These results demonstrate that natural selection can reshape developmental gene expression rapidly, even in highly conserved networks and mechanisms.
NATURE ECOLOGY & EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Wen-Sui Lo, Marianne Roca, Mohannad Dardiry, Marisa Mackie, Gabi Eberhardt, Hanh Witte, Ray Hong, Ralf J. Sommer, James W. Lightfoot
Summary: TGF-beta signaling is essential in nematodes, and it exhibits gene frequency variability and functional divergence, contributing to the generation of novel functions through genetic mutations.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Review
Genetics & Heredity
Barbara J. Meyer
Summary: Abnormalities in chromosome number can disrupt gene expression balance and decrease fitness. The difference in X-chromosome dose used to determine sexual fate is well tolerated across species. The nematode Caenorhabditis elegans provides insights into the chromosome counting and dosage compensation mechanisms and how small quantitative differences in signals can lead to different fates. Understanding X-chromosome dosage compensation reveals the interplay between chromatin modification and chromosome structure in regulating gene expression.
Article
Reproductive Biology
Ana P. Flores Bragulat, Isabel Ortiz, Jaime Catalan, Jesus Dorado, Manuel Hidalgo, Luis Losinno, Marta Dordas-Perpinya, Ivan Yanez-Ortiz, Iris Martinez-Rodero, Jordi Miro, Andres Gambini
Summary: This study investigated the effect of supplementation with jenny preovulatory follicular fluid (PFF) on oocyte developmental competence in donkeys. The results showed that IVM media supplemented with FBS or donkey PFF can promote nuclear maturation and early embryo development in donkeys. This study is the first report of ICSI, time-lapse imaging, and in vitro blastocyst production in donkeys.
Article
Cell Biology
Hongyu Qin, Pengxiang Qu, Huizhong Hu, Wenbin Cao, Hengchao Liu, Yanru Zhang, Jinpeng Zhao, Fatima Nazira, Enqi Liu
Summary: This study demonstrated the important role of sperm-borne small RNAs in embryo development and provided a new strategy for improving the efficiency of SCNT in rabbits.
Article
Obstetrics & Gynecology
Sai Liu, Guoxuan Wu, Li Wang, Yanyan Zhao, Yongqing Lv, Nannan Dang, Yuanqing Yao
Summary: This study aimed to explore the influence of sperm preincubation time on fertilization rate, embryo development and clinical outcomes in in vitro fertilization cycles. The results showed that the optimal preincubation time for prepared sperm before insemination in IVF is one to four hours at 37 degrees C with 6% CO2, which can improve sperm fertilizing ability, blastocyst formation, embryo implantation and cumulative live birth rate.
GINEKOLOGIA POLSKA
(2023)
Article
Agriculture, Dairy & Animal Science
H. Nazari, N. Esfandabadi, A. Shirazi, A. M. Ahadi, E. Ahmadi, A. Safdarinejad
Summary: This study found that the maturation duration of bovine oocytes in vitro affects the sex ratio of resulting embryos. Prolonging the maturation period can increase the blastocyst development rate, and a maturation period of 36 hours generates more male embryos.
IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE
(2022)
Review
Developmental Biology
Gina Broitman-Maduro, Morris F. Maduro
Summary: This study reveals the construction of the gut specification network in Caenorhabditis elegans and related species. It was found that the more complex network in C. elegans differs from a simpler network in more distant species. This research expands our understanding of the regulatory networks in animal embryonic development.
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2023)
Review
Obstetrics & Gynecology
Chinatsu Nagata, Keiko Mekaru, Keiya Gibo, Rie Nakamura, Sugiko Oishi, Maho Miyagi, Kozue Akamine, Yoichi Aoki
Summary: The study showed that differences in fertilization methods, transfer time, and sperm motility rate did not affect the sex ratio of live births through IVF. However, as the number of ICSI and blastocyst transfer cycles increases, factors potentially affecting the sex ratio need further investigation.
JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA
(2021)
Article
Biochemistry & Molecular Biology
Malgorzata Borowiak, Wallis Nahaboo, Martin Reynders, Katharina Nekolla, Pierre Jalinot, Jens Hasserodt, Markus Rehberg, Marie Delattre, Stefan Zahler, Angelika Vollmar, Dirk Trauner, Oliver Thorn-Seshold
Article
Biochemistry & Molecular Biology
Reza Farhadifar, Charles F. Baer, Aurore-Cecile Valfort, Erik C. Andersen, Thomas Mueller-Reichert, Marie Delattre, Daniel J. Needleman
Article
Cell Biology
Wallis Nahaboo, Melissa Zouak, Peter Askjaer, Marie Delattre
MOLECULAR BIOLOGY OF THE CELL
(2015)
Article
Biochemistry & Molecular Biology
Aurore-Cecile Valfort, Caroline Launay, Marie Semon, Marie Delattre
Article
Multidisciplinary Sciences
David Cluet, Pierre-Nicolas Stebe, Soizic Riche, Martin Spichty, Marie Delattre
Article
Biophysics
Helene Bouvrais, Laurent Chesneau, Sylvain Pastezeur, Danielle Fairbrass, Marie Delattre, Jacques Pecreaux
BIOPHYSICAL JOURNAL
(2018)
Article
Multidisciplinary Sciences
Manon Grosmaire, Caroline Launay, Marion Siegwald, Thibault Brugiere, Lilia Estrada-Virrueta, Duncan Berger, Claire Burny, Laurent Modolo, Mark Blaxter, Peter Meister, Marie-Anne Felix, Pierre-Henri Gouyon, Marie Delattre
Article
Evolutionary Biology
Caroline Launay, Marie-Anne Felix, Joris Dieng, Marie Delattre
BMC EVOLUTIONARY BIOLOGY
(2020)
Article
Genetics & Heredity
Hana E. Littleford, Karin Kiontke, David H. A. Fitch, Iva Greenwald
Summary: The bHLH protein HLH-12 is essential for promoting gonadal regulatory cell migration, while its evolution may underlie differences in female gonadal morphology among nematode species. Despite the absence of hlh-12 orthologs in other nematode species, a comprehensive phylogenetic analysis revealed that hlh-12 evolved within the Caenorhabditis clade.
Article
Developmental Biology
Dureen Samandar Eweis, Marie Delattre, Julie Plastino
Summary: The polarity of Diploscapter pachys embryo is achieved during meiosis, seemingly based on the location of the meiotic spindle, by a mechanism that may be present but suppressed in Caenorhabditis elegans.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Dhruv Khatri, Thibault Brugiere, Chaitanya A. Athale, Marie Delattre
Summary: This study observed spindle activities in six different nematode species and found a tradeoff relationship between cytoplasmic viscosity and cortical pulling forces. The study provides a framework for understanding the evolutionary diversification of spindle mobility.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Multidisciplinary Sciences
Irma Tandingan De Ley, Karin Kiontke, Wim Bert, Walter Sudhaus, David H. A. Fitch
Summary: Recently, researchers have focused on the nematode group Phasmarhabditis as a potential source of biocontrol agents for invasive slugs. Pellioditis pelhamensis n. sp. and Pellioditis pellio are two morphologically similar but reproductively isolated species within this group. Phylogenetic analysis confirms that Phasmarhabditis is a junior synonym of Pellioditis. Additionally, Pellioditis bohemica is found to be a junior synonym of Pellioditis pellio, further supporting its association with both slugs and earthworms. These findings suggest that Pellioditis species have a wider range of hosts than just slugs.
Meeting Abstract
Cell Biology
R. Farhadifar, C. F. Baer, A. Valfort, E. Andersen, T. Mueller-Reichert, M. Delattre, D. J. Needleman
MOLECULAR BIOLOGY OF THE CELL
(2014)