Article
Agriculture, Dairy & Animal Science
Seok Hee Lee, Saul Lira-Albarran, Islam M. Saadeldin
Summary: This study provides a comprehensive proteomic analysis of OC-EVs in dogs, revealing the involvement of these proteins in key cellular metabolic processes related to oocyte maturation and embryonic development. These findings offer valuable insights for understanding canine reproductive physiology and establishing an efficient in vitro embryo production system.
Article
Biochemistry & Molecular Biology
Lorena Martin-Jaular, Nathalie Nevo, Julia P. Schessner, Mercedes Tkach, Mabel Jouve, Florent Dingli, Damarys Loew, Kenneth W. Witwer, Matias Ostrowski, Georg H. H. Borner, Clotilde Thery
Summary: This study developed a proteomic profiling approach to study EV subtype composition and identified changes in cellular protein profiles in EVs upon HIV infection. The research also found that SERINC3 plays a role in controlling the surface composition of EVs.
Article
Microbiology
Shih-Chia Yeh, Tania Strilets, Wei-Lian Tan, David Castillo, Hacene Medkour, Felix Rey-Cadilhac, Idalba M. Serrato-Pomar, Florian Rachenne, Avisha Chowdhury, Vanessa Chuo, Sasha R. Azar, Moirangthem Kiran Singh, Rodolphe Hamel, Dorothee Misse, R. Manjunatha Kini, Linda J. Kenney, Nikos Vasilakis, Marc A. Marti-Renom, Guy Nir, Julien Pompon, Mariano A. Garcia-Blanco
Summary: Mosquitoes transmit dengue viruses to humans via infection of skin resident cells at the biting site. In this study, high levels of anti-immune subgenomic flaviviral RNA (sfRNA) were discovered in dengue virus 2-infected mosquito saliva. Salivary sfRNA was found to be protected in detergent-sensitive compartments, likely extracellular vesicles. The presence of sfRNA in salivary vesicles inhibited innate immunity and enhanced dengue virus transmission.
Review
Immunology
Xiaonan Jia, Yiqian Yin, Yiwen Chen, Lingxiang Mao
Summary: Exosomes are membrane-bound vesicles of endocytic origin that contain various biological components and can be regulated by external stimuli, including viruses. Viruses can utilize exosomes to cloak their viral genomes and infect cells, but the mechanisms by which viruses regulate exosomes remain unclear and require further investigation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Oncology
Alicja Gluszko, Miroslaw J. Szczepanski, Theresa L. Whiteside, Torsten E. Reichert, Jacek Siewiera, Nils Ludwig
Summary: Tissue hypoxia is a common feature in HNSCC, leading to alterations in tumor cells. The study found that sEVs released by HNSCC cells under hypoxic conditions have a unique protein composition, which can potentially serve as biomarkers for different degrees of tissue hypoxia and as a resource for anti-angiogenic therapies.
Review
Biochemistry & Molecular Biology
Brett Vahkal, Jamie Kraft, Emanuela Ferretti, Minyoung Chung, Jean-Francois Beaulieu, Illimar Altosaar
Summary: Proteomics can map extracellular vesicles (EVs), such as exosomes, across disease states and cell types, but careful consideration must be given to avoid the impact of contaminants in samples. Human milk EVs and exosomes are increasingly important in health and disease research, yet experimental characterization and functional assays still need further refinement.
Article
Chemistry, Multidisciplinary
Qingfu Zhu, Jiaxin Luo, Hui-Ping Li, Wen Ye, Ke-Qing Shi, Rui Yang, Hao Xu, Hengrui Li, Luke P. Lee, Fei Liu
Summary: We propose a robust AP identification and diagnosis (RAPIDx) method by analyzing the proteomic fingerprints of nanoscale extracellular vesicles (EVs) from clinical samples. We track the circulating biological nano-particles (EVs) released by cells through bottom-up proteomics and establish close phenotype connections between EVs, cell types, and multiple tissues based on their specific proteomes. We identify serum amyloid A (SAA) proteins on EVs as potential biomarkers differentially expressed in AP patients. The RAPIDx platform enables the timely diagnosis and treatment of AP and promotes precision diagnostics and early diagnosis of pancreatic cancer.
Article
Parasitology
Karina P. De Sousa, Jeremy Potriquet, Jason Mulvenna, Javier Sotillo, Penny L. Groves, Alex Loukas, Simon H. Apte, Denise L. Doolan
Summary: This study identified the protein cargo of sEVs in the plasma of P. yoelii-infected mice. The identified proteins include vaccine candidates, potential targets of T cell immunoreactivity, and proteins involved in metabolic processes, regulation, homeostasis, and immunity. These findings enhance our understanding of the host-parasite interactions and provide potential targets for malaria vaccination.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cecilia Lasser, Yasunari Kishino, Kyong-su Park, Ganesh Vilas Shelke, Nasibeh Karimi, Shintaro Suzuki, Lilit Hovhannisyan, Madeleine Radinger, Jan Lotvall
Summary: Studying the proteomes of tissue-derived extracellular vesicles can help identify disease biomarkers and understand cell-to-cell communication. This study isolated EVs from mouse lungs and found changes in their proteomes during allergen-induced airway inflammation, with proteins mainly associated with immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Arada Vinaiphat, Siu Kwan Sze
Summary: Extracellular vesicles (EVs) are lipid bilayer particles released by almost every cell type, carrying proteins, RNAs, etc. that reflect the cellular state and participate in intercellular communication. Recent studies have shown that EVs play a role in neuroprotection and contribute to the onset and progression of neurodegenerative diseases. Therefore, studying EV proteome can be used for the diagnosis and treatment of neurodegenerative diseases.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Article
Biochemistry & Molecular Biology
Kenji Ono, Mikio Niwa, Hiromi Suzuki, Nahoko Bailey Kobayashi, Tetsuhiko Yoshida, Makoto Sawada
Summary: Signal peptides (SPs) play a crucial role in controlling cell functions and can be secreted from cells via extracellular vesicles (EV), potentially influencing intercellular communication.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Zivile Useckaite, Lauren A. Newman, Ashley M. Hopkins, Sonja Klebe, Alex D. Colella, Nusha Chegeni, Ruth Williams, Michael J. Sorich, A. David Rodrigues, Tim K. Chataway, Andrew Rowland
Summary: The study aimed to compare the proteomic profiles of extracellular vesicles (EVs) isolated from liver tissue and the extracellular space. The results showed the potential of liver-derived EVs as a liquid biopsy for understanding the functional status of the liver.
Article
Virology
Victor Acevedo-Sanchez, Roy S. Martinez-Ruiz, Sergio R. Aguilar-Ruiz, Honorio Torres-Aguilar, Pedro Chavez-Olmos, Efrain Garrido, Rafael Baltierrez-Hoyos, Maria de los A. Romero-Tlalolini
Summary: This study compared the proteomic content of extracellular vesicles (EVs) derived from cervical cancer cells and normal cells, and found that EVs play important roles in cell-cell interactions. They are involved in processes such as cell adhesion, proteolysis, lipid metabolism, and immune responses. The role of EVs in cancer may include migration, invasion, metastasis, and the activation or suppression of immune system cells.
Article
Oncology
Magdalena Surman, Urszula Jankowska, Magdalena Wilczak, Malgorzata Przybylo
Summary: This study used shotgun nanoLC-MS/MS to obtain protein profiles of melanoma cells and exosomes. The results provide a deeper understanding of the role of exosomes in melanoma progression and suggest potential biomarkers for circulating melanoma.
