Article
Biochemistry & Molecular Biology
Suyu Yue, Peng Xu, Zhihe Cao, Min Zhuang
Summary: In this study, a new proximity labeling method called PUP-IT2 was established. Compared to traditional methods, PUP-IT2 replaces a larger enzyme with a smaller protein, which avoids interference with the bait protein function. The activity of PUP-IT2 was tested in vitro and in cells, and compared with the original method. Finally, PUP-IT2 coupled mass spectrometry was used to map protein-protein interactions.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Medicinal
Cong Li, Song Liu, Baoyu Dong, Chungen Li, Lunan Jian, Juan He, Jumei Zeng, Qiao Zhou, Da Jia, Youfu Luo, Qingxiang Sun
Summary: In this study, a mutant of Mtb PafA that enabled large-scale purification of active PafA was designed, and two PafA inhibitors were discovered using a high-throughput screening assay. This research provides new tools for tuberculosis research and lays the foundation for PafA-targeted drug development for treating tuberculosis.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Marissa B. Guzzo, Qing Li, Hoang Nguyen, W. Henry Boom, Liem Nguyen
Summary: The study reveals the importance of the Pup-proteasome system in mediating nitric oxide resistance and virulence of Mycobacterium tuberculosis, as well as intrinsic antifolate resistance in both M. tuberculosis and nonpathogenic M. smegmatis. Disruption of Pup or PafA expression enhances susceptibility to antifolates in M. smegmatis, while cross-species expression of M. tuberculosis homologs restores antifolate resistance in M. smegmatis mutants. Targeted deletion of structural components of PPS proteolytic core also reduces antifolate resistance, highlighting the role of PPS in protecting mycobacteria from antimicrobial antifolates.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Matthias F. Block, Cyrille L. Delley, Lena M. L. Keller, Timo T. Stuehlinger, Eilika Weber-Ban
Summary: Pupylation is a post-translational modification found in Mycobacterium tuberculosis and other Actinobacteria, which involves the attachment of prokaryotic ubiquitin-like protein (Pup) to lysines on target proteins by the ligase PafA. Proteomic studies suggest that hundreds of potential pupylation targets are modified by PafA. This study shows that the availability of surface lysines alone is not sufficient for interaction between PafA and target proteins, and identifies the structural recognition motif involved in PafA's recognition of authentic substrates.
NATURE COMMUNICATIONS
(2023)
Article
Endocrinology & Metabolism
Henning Tim Langer, Samuel R. Taylor, Mujmmail Ahmed, Tiffany Perrier, Tanvir Ahmed, Marcus D. Goncalves
Summary: Inhibition of the UPS can block weight loss and tissue catabolism during fasting in mice. This is achieved through a combined effect of degradation and synthesis of biomolecules.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2023)
Article
Dermatology
Julie Briot, Eric Arbey, Dominique Goudouneche, Dominique Bernard, Michel Simon, Marie-Claire Mechin
Summary: The absence of a functional proteasome in the suprabasal layers of the epidermis causes keratosis linearis with ichthyosis congenita and sclerosing keratoderma syndrome. Inhibited proteasome proteolytic activity leads to down-regulation of filaggrin expression and perturbation of urocanic acid and pyrrolidone carboxylic acid production. Accumulation of small cytoplasmic vesicles and activation of autophagy markers were observed upon proteasome inhibition.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Indrajit Sahu, Monika Bajorek, Xiaolin Tan, Madabhushi Srividya, Daria Krutauz, Noa Reis, Pawel A. Osmulski, Maria E. Gaczynska, Michael H. Glickman
Summary: The active sites of the 26S proteasome are located in the catalytic chamber of its 20S core particle. Access to this chamber is through a narrow channel formed by the seven outer alpha subunits. The gate blocking this channel is formed by the N-termini of neighboring alpha subunits, and attachment of activators rearranges these N-termini, allowing entry of substrates. A hydrogen bond between an invariant tyrosine (Y) in each subunit and a conserved aspartate (D) in its counterclockwise neighbor stabilizes the open state, except for the alpha 1-alpha 2 pair which leaves a gap in the ring. The third residue (X) of the YD(X) motif aligns with the open channel, and deletion of the alpha 2 N-terminal tail attenuates proteolysis despite the appearance of an open gate state.
Review
Biochemistry & Molecular Biology
Anjali Pande, Bong-Gyu Mun, Murtaza Khan, Waqas Rahim, Da-Sol Lee, Geun-Mo Lee, Tiba Nazar Ibrahim Al Azawi, Adil Hussain, Byung-Wook Yun
Summary: The versatility of nitric oxide as a signaling molecule in plant biology involves post-translational modifications of target proteins and their subsequent proteasomal degradation through ubiquitylation. The close link between NO-mediated PTMs and ubiquitylation highlights the intricate mechanisms of NO signal perception and transduction. This review provides insights into the pathways connecting NO-PTMs with ubiquitylation, shedding light on the regulation of protein degradation and activity in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shion Osana, Yasuo Kitajima, Suzuki Naoki, Hiroaki Takada, Kazutaka Murayama, Yutaka Kano, Ryoichi Nagatomi
Summary: The proteasome and aminopeptidases are thought to be involved in protein synthesis through intracellular energy production by recycled-amino acid metabolism, thereby maintaining myoblast integrity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Plant Sciences
Yi Sun, R. Paul Jarvis
Summary: Chloroplasts are plant organelles responsible for photosynthesis and other vital functions, and their complex proteome is controlled by diverse processes of protein translocation and degradation. Most chloroplast proteins are imported from the cytosol and then sorted to different organellar subcompartments. Chloroplast proteolysis involves proteases of prokaryotic origin and the nucleocytosolic ubiquitin-proteasome system, which regulate protein import and reconfigure chloroplast functions in response to developmental and environmental signals.
ANNUAL REVIEW OF PLANT BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Philip Coffino, Yifan Cheng
Summary: Allostery-regulation at distant sites is a crucial concept in biology, and the proteasome provides multiple examples of this regulation. It can span a long distance, influencing the interactions and assembly of proteasome modules, and explain how diverse populations of proteasome assemblies are built and maintained in cells.
Review
Pharmacology & Pharmacy
Boya Chen, Haiying Zhu, Bo Yang, Ji Cao
Summary: Immunoproteasome is a variant of proteasome optimized for producing antigenic peptides with higher binding affinity and plays a dual role in different types of cancer. It is involved in antigen presentation and degradation of proteins, including tumor suppressor proteins, contributing to tumor progression. Targeting immunoproteasome could be a potential therapeutic intervention in cancer treatment and understanding its role is crucial for cancer prevention and treatment.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Multidisciplinary Sciences
Hengjun Cui, Andreas U. Mueller, Marc Leibundgut, Jiawen Tian, Nenad Ban, Eilika Weber-Ban
Summary: This study presents crystal structures of Dop in different functional states, providing insights into the catalytic mechanism of this enzyme. Pupylation and depupylation are reversible protein modification processes mainly acting in members of Actinobacteria.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Guido Janssen, Susan Zhang, Remco Merkx, Christa Schiesswohl, Champak Chatterjee, K. Heran Darwin, Paul P. Geurink, Gerbrand J. van der Heden van Noort, Huib Ovaa
Summary: Tuberculosis is a global health problem caused by Mycobacterium tuberculosis infection. New drugs targeting Mtb are urgently needed due to the existence of drug resistant strains. The Pup proteasome system has been identified as a potential target for drug development, with inhibitors of Dop and PafA showing promising results in inhibiting Mtb activity.
Article
Medicine, Research & Experimental
Carlos Alberto Arcaro, Renata Pires Assis, Juliana Oriel Oliveira, Neusa Maria Zanon, Silvia Paula-Gomes, Luiz Carlos Carvalho Navegantes, Isis Carmo Kettelhut, Iguatemy Lourenco Brunetti, Amanda Martins Baviera
Summary: The study showed that rolipram, a selective PDE4 inhibitor, can inhibit protein breakdown in skeletal muscles of diabetic rats by activating cAMP downstream effectors, ultimately leading to the preservation of muscle mass.
Article
Biochemistry & Molecular Biology
Alexander Aranovich, Shani Braier-Marcovitz, Esti Ansbacher, Rony Granek, Abraham H. Parola, Itzhak Fishov
BIOSCIENCE REPORTS
(2015)
Review
Microbiology
Kouji Matsumoto, Hiroshi Hara, Itzhak Fishov, Eugenia Mileykovskaya, Vic Norris
FRONTIERS IN MICROBIOLOGY
(2015)
Article
Microbiology
Pramod Kumar, Amarjeet Yadav, Itzhak Fishov, Mario Feingold
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Biophysics
Galina Reshes, Sharon Vanounou, Itzhak Fishov, Mario Feingold
BIOPHYSICAL JOURNAL
(2008)
Article
Biochemistry & Molecular Biology
Shirley Mazor, Sharon Vanounou, Itzhak Fishov
CHEMISTRY AND PHYSICS OF LIPIDS
(2012)
Review
Microbiology
Itzhak Fishov, Vic Norris
CURRENT OPINION IN MICROBIOLOGY
(2012)
Article
Microbiology
Oreste Piro, Gideon Carmon, Mario Feingold, Itzhak Fishov
ENVIRONMENTAL MICROBIOLOGY
(2013)
Article
Optics
G. Carmon, I. Fishov, M. Feingold
Article
Multidisciplinary Sciences
Tomer Regev, Nadav Myers, Raz Zarivach, Itzhak Fishov
Article
Microbiology
Carmen Mata Martin, Arieh Zaritsky, Itzhak Fishov, Elena C. Guzman
Article
Biophysics
Itay Gelber, Alexander Aranovich, Mario Feingold, Itzhak Fishov
Summary: The process of segregating the replicating chromosome from one to two nucleoid bodies in bacterial cells is regulated by interactions with cellular processes, with internal stochasticity possibly contributing to cell-to-cell phenotypic variability. Comparing dividing cell lineages with filamentous cells, it was found that removing boundaries between nucleoids can reduce variability in chromosome segregation dynamics.
BIOPHYSICAL JOURNAL
(2021)
Editorial Material
Multidisciplinary Sciences
Itzhak Fishov, Sharanya Namboodiri
Summary: The type three secretion system (T3SS) is a membrane-anchored nano-machine used by pathogenic bacteria to inject effector proteins and control host cells. Recent research shows the colocalization of T3SS-encoding locus, its transcriptional activators, protein products, and the complete structure at the cell membrane, providing evidence for a mechanism called 'transertion'.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Anteneh Hailu Abebe, Alexander Aranovich, Itzhak Fishov
FEMS MICROBIOLOGY LETTERS
(2017)
Article
Biochemistry & Molecular Biology
R. Tsukanov, G. Reshes, G. Carmon, E. Fischer-Friedrich, N. S. Gov, I. Fishov, M. Feingold
