Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 134, Issue -, Pages 335-342Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2019.01.017
Keywords
RNA-Seq; Ontogeny; Mouse liver; Antioxidant
Funding
- National Institutes of Health (NIH) [GM111381, ES019487, ES025708, GM118367]
- University of Washington Center for Exposures, Diseases, Genomics, and Environment [P30 ES0007033]
- Murphy Endowment
Ask authors/readers for more resources
Mammals have developed a variety of antioxidant systems to protect them from the oxygen environment and toxic stimuli. Little is known about the mRNA abundance of antioxidant components during postnatal development of the liver. Therefore, the purpose of this study was to compare the mRNA abundance of antioxidant components during liver development. Livers from male C57BL/6J mice were collected at 12 ages from prenatal to adulthood. The transcriptome was determined by RNA-Seq with transcript abundance estimated by Cufflinks. RNA-Seq provided a complete, more accurate, and unbiased quantification of the transcriptome. Among 33 known antioxidant components examined, three ontogeny patterns of liver antioxidant components were observed: (1) Prenatal-enriched, in which the mRNAs decreased from fetal livers to adulthood, such as metallothionein and heme oxygenase-1; (2) adolescent-rich and relatively stable expression, such as peroxiredoxins; and (3) adult-rich, in which the mRNA increased with age, such as catalase and superoxide dismutase. Alternative splicing of several antioxidant genes, such as Keap1, Glrx2, Gpx3, and Txnrd1, were also detected by RNA-Seq. In summary, RNA-Seq revealed the relative abundance of hepatic antioxidant enzymes, which are important in protecting against the deleterious effects of oxidative stress.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available