Article
Biochemistry & Molecular Biology
James F. Markworth, Kristoffer B. Sugg, Dylan C. Sarver, Krishna Rao Maddipati, Susan V. Brooks
Summary: This study found that tendon overuse can lead to a delayed and prolonged local inflammatory response, characterized by an increase in pro-inflammatory eicosanoids and a relative lack of specialized pro-resolving lipid mediators.
Article
Multidisciplinary Sciences
Xiaoyu Zhou, Zhuofan Wang, Jingjin Fan, Zheng Ouyang
Summary: Ion mobility is utilized in mass spectrometers for analyzing the structure of biomolecules. Researchers demonstrate that ion mobility analysis in an ion trap with ultra-high fields can achieve resolutions over 10,000 for isomer separation, as shown for disaccharide, phospholipid, and peptide isomers. Elucidating complex structures of biomolecules is crucial in chemistry and life sciences. In the last decade, ion mobility coupled with mass spectrometry has become a unique tool for distinguishing isomers and isoforms of biomolecules. This study presents a concept for ion mobility analysis using an ion trap, enabling ultra-high field isomer separation and achieving resolutions over 10,000. The potential of this technology is demonstrated for analyzing isomers of disaccharides, phospholipids, and peptides with post-translational modifications.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Sanaz C. Habibi, Gabe Nagy
Summary: In recent years, ion mobility spectrometry-mass spectrometry (IMS-MS) has advanced omics-based research with high-resolution platforms, but most separations have been qualitative. The rotational averaged ion neutral collision cross section (CCS) is a quantitative metric for characterizing biomolecules in IMS-MS. Determining CCS in multipass IMS systems has been challenging due to a lack of methods for calculating CCS and the difficulty of requiring identical passes for calibrants and unknown compounds. This study presents a method using average ion velocities to calculate CCS values in cyclic IMS-MS separations, demonstrating good agreement with previous literature and potential applicability to other IMS-MS platforms.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Vijaykumar Patra, Natalie Bordag, Yohann Clement, Harald Koefeler, Jean-Francois Nicolas, Marc Vocanson, Sophie Ayciriex, Peter Wolf
Summary: Skin metabolites (< 1500 Da) are crucial for various skin functions and their interactions with microbiome and UV exposure were investigated in this study. Germ-free, disinfected, and control mice were exposed to immunosuppressive doses of UVB radiation, and lipidome and metabolome profiling of their skin tissue were performed. UV differentially regulated metabolites and membrane lipid species, showing a microbiome-dependent effect. These findings provide insights into the dynamics of skin metabolome, microbiome, and UV exposure, and have implications for the development of skin health applications based on metabolites or lipids.
SCIENTIFIC REPORTS
(2023)
Article
Ophthalmology
Simin Masoudi, Todd W. Mitchell, Mark D. Willcox
Summary: This study found that levels of certain TAG and WE species decreased during the day without contact lens wear, while levels of CE and WE increased from morning to evening with contact lens wear. The level of non-polar lipids did not correlate with ocular comfort.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Engineering, Biomedical
Bin Jiao, Wei Zhou, Yikun Liu, Wenpeng Zhang, Zheng Ouyang
Summary: In this study, a polyporous polymeric membrane (PPM) microprobe was developed for efficient sampling of lipids directly from tissue samples. The microprobe has high recoveries and chemical stability, making it suitable for biomedical applications.
MATERIALS TODAY BIO
(2022)
Review
Biochemistry & Molecular Biology
Neetika Jaisinghani, Jessica C. Seeliger
Summary: Studying bacterial lipids poses challenges due to their differences from eukaryotic lipids, requiring specific methods for analysis. Recent research has made progress in this area but still faces challenges.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Matias Cabruja, Josefina Priotti, Pablo Domizi, Katharina Papsdorf, Deanna L. Kroetz, Anne Brunet, Kevin Contrepois, Michael P. Snyder
Summary: TriP-MS3 is a mass spectrometry-based method that allows for comprehensive qualitative and semiquantitative profiling of over 6,700 TAG species. This method demonstrated excellent reproducibility and linearity, detecting 285 individual TAG species in human plasma. The accuracy of the method was validated by comparison with a state-of-the-art reverse phase liquid chromatography (RPLC)-MS.
ANALYTICA CHIMICA ACTA
(2021)
Review
Biochemical Research Methods
Sven Heiles
Summary: Metabolomics and lipidomics are driving forces in the omics era, with tandem mass spectrometry methods playing a crucial role in characterizing the structural details of metabolites and lipids. Recent advancements in alternative tandem MS methodologies aim to provide full structural characterization of these compounds. The review critically examines the principles, capabilities, drawbacks, and initial applications of these advanced tandem mass spectrometry strategies in the fields of metabolomics and lipidomics.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Taiga Iwama, Kuniyuki Kano, Daisuke Saigusa, Kim Ekroos, Gerhild van Echten-Deckert, Johannes Vogt, Junken Aoki
Summary: The novel on-tissue derivatization method using Phos-tag in combination with MALDI-MSI enables visualization of low-abundance bioactive lipids like LPA and S1P in the brain, providing a simple and optimal way to reveal the spatial localization of these important molecules in tissues.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dong Hao Wang, Hui Gyu Park, Zhen Wang, R. J. Scott Lacombe, Vadim V. Shmanai, Andrei Bekish, Karsten Schmidt, Mikhail S. Shchepinov, J. Thomas Brenna
Summary: This study introduces a novel method for quantitatively determining the deuterium content along a hydrocarbon chain. By utilizing deuteration reactions and analysis techniques, the method enables accurate quantification of deuterium abundance at different positions of D-HUFA.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Hongwei Li, Xue Li, Qian Wu, Xing Wang, Zhonghua Qin, Yaguo Wang, Yanbin He, Qi Wu, Li Li, Huaiyong Chen
Summary: In COVID-19 survivors, about 41% reported at least one clinical symptom and 26.5% exhibited lung imaging abnormalities 6 months after discharge. Plasma proteomics and metabolomics analysis showed that COVID-19 survivors differed from healthy controls in terms of extracellular matrix, immune response, and hemostasis pathways. Moreover, COVID-19 survivors also exhibited abnormal lipid metabolism, disordered immune response, and changes in pulmonary fibrosis-related proteins.
CELL DEATH & DISEASE
(2022)
Review
Food Science & Technology
Wei Ren, Mengqi Sun, Xiaoyuan Shi, Tianqi Wang, Yonghui Wang, Changfa Wang, Mengmeng Li
Summary: Lipids are crucial for biological functions, but their complex structure and limited analytical techniques have hindered the understanding of their mechanisms. Advances in mass spectrometry and bioinformatics have enabled rapid detection and analysis of lipids using MS-based lipidomic techniques. Milk lipids, as complex metabolites, are important for human health. This review discusses the applications of lipidomic techniques in dairy products, including compositional analysis, quality and authenticity identification, and origin identification, aiming to provide technical support for dairy product development.
Article
Microbiology
Ekaterina R. Kotlova, Svetlana V. Senik, Bairta S. Manzhieva, Anna A. Kiyashko, Natalia V. Shakhova, Roman K. Puzansky, Sergei V. Volobuev, Alexander D. Misharev, Eugeny B. Serebryakov, Nadezhda V. Psurtseva
Summary: Phosphatidylcholines (PC) are the main membrane lipid constituents, synthesized and remodeled in basidiomycetes. The molecular profile of PC is determined by genetic features and environment, with a diverse range of molecular species. Hierarchical cluster and principal component analyses revealed four main types of PC profiles, independent of taxonomic position and ecology.
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Ophthalmology
Padmapriya Ramamoorthy, Safal Khanal, Jason J. Nichols
Summary: This study found that the tear film of individuals with contact lens-related dry eye (CLDE) contains increased levels of several inflammatory mediators. These results support the potential role of inflammation in CLDE.
CONTACT LENS & ANTERIOR EYE
(2022)
Article
Ophthalmology
Nathan Efron, Philip B. Morgan, Lyndon W. Jones, Jason J. Nichols
Summary: This study utilized bibliometrics to analyze the impact of keratoconus literature, identifying highly cited papers, impactful authors, institutions, countries, and journals in the field. Keratoconus is a topic of great interest in clinical and scientific research, with significant contributions from authors, institutions, and countries, particularly in the United States.
CLINICAL AND EXPERIMENTAL OPTOMETRY
(2022)
Article
Ophthalmology
Jason J. Nichols, Philip B. Morgan, Nathan Efron, Lyndon W. Jones
Summary: This study identified and ranked a group of outstanding scientists in the field of optometry, highlighting their significant contributions to optometric research. Additionally, the distribution of optometrists from different countries worldwide and the prominent role of certain universities in this field were demonstrated in the results.
CLINICAL AND EXPERIMENTAL OPTOMETRY
(2022)
Article
Immunology
Kwaku A. Osei, Joshua L. Mieher, Manisha Patel, Jason J. Nichols, Champion Deivanayagam
Summary: This study investigates the adhesion of tear protein Gp340 and its domains on contact lens materials and its effects on bacterial adherence, finding that Gp340 and its SRCR domains play an important role in bacterial adhesion to contact lenses.
INFECTION AND IMMUNITY
(2022)
Article
Ophthalmology
Safal Khanal, Yuqiang Bai, William Ngo, Kelly K. Nichols, Landon Wilson, Stephen Barnes, Jason J. Nichols
Summary: The study evaluated the presence and roles of cholesteryl esters (CEs) and wax esters (WEs) from human tear film and meibum in meibomian gland dysfunction (MGD). Results showed that the abundances of CEs and WEs from human precorneal tear film and meibum were independent of MGD disease status and tear film thinning rates. Changes in CEs and WEs do not contribute to alterations in tear film dynamics in MGD.
Article
Ophthalmology
Nathan Efron, Lyndon W. Jones, Phillip B. Morgan, Jason J. Nichols
Summary: Publication metrics were derived for silicone hydrogel (SH) and daily disposable (DD) contact lenses fields, identifying the most impactful authors, institutions, countries, and journals in each category. The study found that the impact of SH field is significantly higher than that of the DD field.
JOURNAL OF OPTOMETRY
(2022)
Article
Cell & Tissue Engineering
Jasmin Taubenschmid-Stowers, Maria Rostovskaya, Fatima Santos, Sebastian Ljung, Ricard Argelaguet, Felix Krueger, Jennifer Nichols, Wolf Reik
Summary: This study discovered human 8-cell-like cells (8CLCs) among naive embryonic stem cells, which transcriptionally resemble the 8-cell human embryo. These cells express markers and elements associated with embryonic development, providing important insights into early events in human embryogenesis.
Article
Ophthalmology
Eugene Appenteng Osae, Lyndon Jones, Jason J. Nichols
Summary: This review summarizes the evidence on how contact lens wear affects the morphology and secretion of the meibomian glands, with key factors such as duration, type, design, and material of the lenses influencing the extent of these changes.
Article
Ophthalmology
Yuqiang Bai, William Ngo, Safal Khanal, Jason J. Nichols
Summary: The thickness of the tear film lipid layer (TFLL) in meibomian gland dysfunction (MGD) was evaluated using a high-resolution optical microscope. The results showed that the MGD and mixed groups had the largest percentages of TFLL thicknesses fall within the thinnest modes. Measures of central tendency do not fully appreciate the variable distributions of TFLL across disease spectra.
Article
Ophthalmology
Jianzhong Chen, Dongfeng Cao, Seth D. Fortmann, Christine A. Curcio, Richard M. Feist, Jason N. Crosson
Summary: Understanding the protein composition of subretinal fluid could lead to new treatment strategies for blindness. The study found that subretinal fluid contains abundant proteins, with transthyretin (TTR) being one of the major proteins. The research also highlighted the importance of glutathionylation in the normal function of TTR.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Biology
Sophie Kraunsoe, Takuya Azami, Yihan Pei, Graziano Martello, Kenneth Jones, Thorsten Boroviak, Jennifer Nichols
Summary: We investigated the roles of STAT3 and TFCP2L1 in maintaining naive pluripotency and found that their protein levels decline during blastocyst expansion but increase in the embryonic region after diapause induction. Embryos lacking STAT3 or TFCP2L1 showed catastrophic loss of the inner cell mass during diapause, indicating additional signals are required for sustaining pluripotency. Blocking MEK/ERK signaling allowed efficient derivation of embryonic stem cells from STAT3 null embryos, highlighting an unknown role of TFCP2L1 in the transition to embryonic stem cells in vitro.
Article
Developmental Biology
Elena Corujo-Simon, Arthur H. Radley, Jennifer Nichols
Summary: The rapid establishment of three founder lineages, namely trophectoderm, epiblast and hypoblast, is crucial for successful human pregnancy. Different models have been proposed to explain the lineage segregation. This study investigates the expression order of genes associated with hypoblast emergence and supports the model of sequential segregation of founder lineages. Specifically, PDGFRA is the first marker identifying presumptive hypoblast, followed by SOX17, FOXA2, and GATA4.
Editorial Material
Ophthalmology
Kwaku A. Osei, Champion Deivanayagam, Jason J. Nichols
Article
Ophthalmology
Jason J. Nichols, Philip B. Morgan, Lyndon W. Jones, Nathan Efron
Summary: This study conducted a bibliometric analysis of ophthalmic journals and identified the most highly cited articles, leading journals, authors, institutions, and countries. It provides guidance for individuals or institutions regarding their contributions to the field of ophthalmology.
JAMA OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Nathan Efron, Philip B. Morgan, Lyndon W. Jones, Jason J. Nichols
Summary: This study identifies the most impactful journals, papers, authors, institutions, and countries that cite optometry journal articles, confirming the utility and influence of optometry journals.
JOURNAL OF OPTOMETRY
(2023)
