Review
Biochemistry & Molecular Biology
Ksenia Maslenkina, Liudmila Mikhaleva, Maxim Naumenko, Rositsa Vandysheva, Michail Gushchin, Dmitri Atiakshin, Igor Buchwalow, Markus Tiemann
Summary: Barrett's esophagus (BE) is a premalignant lesion that can develop into esophageal adenocarcinoma (EAC). The development of BE is caused by biliary reflux and involves various stem cell origins. The concept of healing has been replaced by the cytokine storm and inflammatory microenvironment, leading to intestinal metaplasia. This review discusses the roles of molecular pathways in the pathogenesis of BE and EAC, including NOTCH, hedgehog, NF-?B, and IL6/STAT3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Anshuman Panda, Gyan Bhanot, Shridar Ganesan, Manisha Bajpai
Summary: Stable cell lines derived from primary tissues and tumors are commonly used in medical research. This study evaluated gene expression profiles of esophageal tissues and cell lines, finding discrepancies in the gene expression profiles of Barrett's esophagus (BE) cell lines compared to primary BE tissues and esophageal adenocarcinoma (EAC) tumors. Exposure to an acidic bile environment did not alter the squamous-like gene expression pattern of a BE cell line. The findings underscore the importance of carefully selecting appropriate in vitro tools for studying esophageal adenocarcinoma.
Article
Oncology
Yue Chen, Chenyu Sun, Yile Wu, Xin Chen, Sujatha Kailas, Zeid Karadsheh, Guangyuan Li, Zhichun Guo, Hongru Yang, Lei Hu, Qin Zhou
Summary: The use of PPI is negatively associated with the risk of progression to HGD/EAC in BE patients, and the risk of HGD/EAC can be reduced with the prolonged use of PPI. Sensitivity analysis showed the stable results of this meta-analysis, and no publication bias was detected.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pelin Ergun, Sezgi Kipcak, Serhat Bor
Summary: Barrett's esophagus (BE) is a condition resulting from chronic gastroesophageal reflux disease, which may progress to esophageal adenocarcinoma (EAC). The histological dysplasia grade is commonly used as a biomarker, but its effectiveness is limited due to cost and lack of progression in many BE patients. Clinicians require multiple or more quantitative biomarkers for early diagnosis of EAC, which has high mortality. Epigenetic factors in the early stages of neoplastic transformation show promise as predictive biomarkers. This review summarizes current research on DNA methylations, histone modifications, and noncoding RNAs (miRNAs) during the progression from BE dysplasia to EAC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chengjiao Yao, Yilin Li, Lihong Luo, Qin Xiong, Xiaowu Zhong, Fengjiao Xie, Peimin Feng
Summary: The study identified differentially expressed miRNAs (DEMs) and genes (DEGs) between EAC and BE tissue samples, performed pathway and functional enrichment analysis, and identified enriched functions and pathways. Some hub genes, such as GART and TNFSF11, as well as highly connected miRNAs like hsa-miR-143 and hsa-miR-133b were determined. This research provides insights into the development mechanism of BE and EAC.
Article
Oncology
Lei Chen, Farah Ballout, Heng Lu, Tianling Hu, Shoumin Zhu, Zheng Chen, Dunfa Peng
Summary: In this study, the gene expression of NEK family members in esophageal adenocarcinoma and its precancerous condition was analyzed. The findings suggest that upregulation of certain NEKs may be important in the development of esophageal adenocarcinoma.
Article
Gastroenterology & Hepatology
Tarek Sawas, Saam Dilmaghani, Lovekirat Dhaliwal, Kenneth K. Wang, Prasad G. Iyer, David A. Katzka
Summary: The study found that compared to BE patients, EAC patients had significantly lower rates of heartburn, diabetes, hyperlipidemia, hypertension, nonalcoholic steatohepatitis, and metabolic syndrome. Therefore, optimal strategies for screening for prevalent EAC may need to be adjusted.
AMERICAN JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Genetics & Heredity
Manisha Bajpai, Anshuman Panda, Kristen Birudaraju, James Van Gurp, Amitabh Chak, Kiron M. Das, Parisa Javidian, Hana Aviv
Summary: Barrett's esophagus (BE) is a premalignant metaplasia in patients with chronic gastroesophageal reflux disease (GERD), which can progress to esophageal adenocarcinoma (EA). A novel chromosomal translocation t(10:16) was discovered, and a FISH-EA assay was designed to detect these chromosomal events.
FRONTIERS IN GENETICS
(2021)
Article
Multidisciplinary Sciences
Nan Yi, Hailiang Zhao, Juan He, Xike Xie, Liexin Liang, Guowen Zuo, Mingyue Xiong, Yunxiao Liang, Tingzhuang Yi
Summary: Almost 50% of esophageal adenocarcinoma (EAC) patients are progressed from Barrett's esophagus (BE). However, effective stratification and therapy methods for BE and EAC are still lacking. Differentially expressed genes (DEGs) between BE and EAC were identified through the analysis of two public datasets (GSE26886 and GSE37200), followed by bioinformatics analyses to explore potential biomarkers associated with BE-EAC. The results revealed a number of up- and down-regulated genes, which were highly involved in tumorigenesis according to the GO and KEGG enrichment analysis. In addition, the study identified several potential diagnostic and prognostic biomarkers in BE-EAC.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Yuhan Hao, Ulas Karaoz, Liying Yang, Patrick S. Yachimski, Wenzhi Tseng, Carlos W. Nossa, Weimin Ye, Mengkao Tseng, Michael Poles, Fritz Francois, Morris Traube, Stuart M. Brown, Yu Chen, Manolito Torralba, Richard M. Peek, Eoin L. Brodie, Zhiheng Pei
Summary: The incidence of esophageal adenocarcinoma (EA) in the United States has increased significantly since the 1970s, and the reasons for this are still unclear. This study suggests that the widespread use of antibiotics may have increased the procarcinogenic potential of the orodigestive microbiota, leading to the development of gastroesophageal reflux (GR), Barrett's esophagus (BE), and EA. By analyzing the microbiota in the mouth, esophagus, stomach, and rectum, the researchers discovered significant differences in the abundance of microbial taxa between normal controls (NC) and the three disease phenotypes. They also found that the oral and esophageal microbiota became more diverse along the sequence of GR -> BE -> EA, with certain genera progressively depleted or enriched. In addition, gene functional content analysis revealed an enrichment of genes related to antibiotic resistance, immune response, nitrate-nitrite-nitric oxide pathway, and acetaldehyde metabolism throughout the stages of EA development. These findings suggest that the orodigestive microbiota undergoes dysbiosis and microbial gene changes during the development of EA, and further research is needed to understand their roles in EA pathogenesis.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Multidisciplinary Sciences
N. Carrossini, N. Meireles Da Costa, E. Andrade-Barreto, V. P. L. Sousa, P. Nicolau-Neto, P. T. Souza-Santos, G. R. Mansur, L. Wernersbach, P. T. Bozza, J. P. B. Viola, Luis Felipe Ribeiro Pinto
Summary: Esophageal cancer presents two main histological subtypes, with ESCC mainly associated with tobacco and alcohol abuse, and EAC and BE linked to obesity and chronic GERD. Obesity triggers LD accumulation in non-adipose tissues, which may contribute to the production and action of inflammatory mediators, thus promoting the genesis and progression of BE and EAC.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Mamoru Tokunaga, Kenichiro Okimoto, Naoki Akizue, Kentaro Ishikawa, Yosuke Hirotsu, Kenji Amemiya, Masayuki Ota, Keisuke Matsusaka, Motoi Nishimura, Kazuyuki Matsushita, Tsubasa Ishikawa, Ariki Nagashima, Wataru Shiratori, Tatsuya Kaneko, Hirotaka Oura, Kengo Kanayama, Yuki Ohta, Takashi Taida, Keiko Saito, Tomoaki Matsumura, Tetsuhiro Chiba, Hitoshi Mochizuki, Makoto Arai, Jun Kato, Jun-ichiro Ikeda, Masao Omata, Naoya Kato
Summary: This study investigated the genetic characteristics of nondysplastic Barrett's esophagus (NDBE) to early esophageal adenocarcinoma (EAC) in the Japanese population. The results showed that the genetic variants of TP53 in Japanese early EAC were similar to those in western countries, and TP53 putative drivers were even detected in Japanese patients with nondysplastic short-segment Barrett's esophagus, indicating a potential higher risk of progression. Proper follow-ups may be necessary for patients with short-segment Barrett's esophagus to monitor the risk of progression to high-grade dysplasia or EAC.
SCIENTIFIC REPORTS
(2021)
Review
Oncology
Franz Ludwig Dumoulin, Fabian Dario Rodriguez-Monaco, Alanna Ebigbo, Ingo Steinbruck
Summary: Esophageal adenocarcinoma is the most common subtype of esophageal cancer in Western societies and its incidence is increasing. The development and validation of artificial intelligence systems in the field of gastroenterology may help address unresolved issues in the prevention and surveillance of this cancer.
Review
Gastroenterology & Hepatology
Motasem Alkhayyat, Prabhat Kumar, Krishna O. Sanaka, Prashanthi N. Thota
Summary: The incidence of Barrett's esophagus and esophageal adenocarcinoma has been increasing, leading to a search for chemoprevention strategies. Proton-pump inhibitors and Aspirin have shown effectiveness in reducing cancer incidence in Barrett's patients. Other agents lack sufficient evidence for chemoprevention in these patients.
THERAPEUTIC ADVANCES IN GASTROENTEROLOGY
(2021)
Article
Multidisciplinary Sciences
Chunyang Bao, Richard W. Tourdot, Gregory J. Brunette, Chip Stewart, Lili Sun, Hideo Baba, Masayuki Watanabe, Agoston T. Agoston, Kunal Jajoo, Jon M. Davison, Katie S. Nason, Gad Getz, Kenneth K. Wang, Yu Imamura, Robert Odze, Adam J. Bass, Matthew D. Stachler, Cheng-Zhong Zhang
Summary: By analyzing chromosomal copy-number evolution in early cancers and precancerous lesions of the esophagus, the authors reveal signatures of ongoing chromosomal instability and its role in promoting tumor progression.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Ravindra Uppaluri, Katie M. Campbell, Ann Marie Egloff, Paul Zolkind, Zachary L. Skidmore, Brian Nussenbaum, Randal C. Paniello, Jason T. Rich, Ryan Jackson, Patrik Pipkorn, Loren S. Michel, Jessica Ley, Peter Oppelt, Gavin P. Dunn, Erica K. Barnell, Nicholas C. Spies, Tianxiang Lin, Tiantian Li, David T. Mulder, Youstina Hanna, Iulia Cirlan, Trevor J. Pugh, Tenny Mudianto, Rachel Riley, Liye Zhou, Vickie Y. Jo, Matthew D. Stachler, Glenn J. Hanna, Jason Kass, Robert Haddad, Jonathan D. Schoenfeld, Evisa Gjini, Ana Lako, Wade Thorstad, Hiram A. Gay, Mackenzie Daly, Scott J. Rodig, Ian S. Hagemann, Dorina Kallogjeri, Jay F. Piccirillo, Rebecca D. Chernock, Malachi Griffith, Obi L. Griffith, Douglas R. Adkins
CLINICAL CANCER RESEARCH
(2020)
Correction
Oncology
Ravindra Uppaluri, Katie M. Campbell, Ann Marie Egloff, Paul Zolkind, Zachary L. Skidmore, Brian Nussenbaum, Randal C. Paniello, Jason T. Rich, Ryan Jackson, Patrik Pipkorn, Loren S. Michel, Jessica Ley, Peter Oppelt, Gavin P. Dunn, Erica K. Barnell, Nicholas C. Spies, Tianxiang Lin, Tiantian Li, David T. Mulder, Youstina Hanna, Iulia Cirlan, Trevor J. Pugh, Tenny Mudianto, Rachel Riley, Liye Zhou, Vickie Y. Jo, Matthew D. Stachler, Glenn J. Hanna, Jason Kass, Robert Haddad, Jonathan D. Schoenfeld, Evisa Gjini, Ana Lako, Wade Thorstad, Hiram A. Gay, Mackenzie Daly, Scott J. Rodig, Ian S. Hagemann, Dorina Kallogjeri, Jay F. Piccirillo, Rebecca D. Chernock, Malachi Griffith, Obi L. Griffith, Douglas R. Adkins
CLINICAL CANCER RESEARCH
(2021)
Article
Genetics & Heredity
Zhong Wu, Jin Zhou, Xiaoyang Zhang, Zhouwei Zhang, Yingtian Xie, Jie bin Liu, Zandra Ho, Arpit Panda, Xintao Qiu, Paloma Cejas, Israel Canadas, Fahire Goknur Akarca, James M. McFarland, Ankur K. Nagaraja, Louisa B. Goss, Nikolas Kesten, Longlong Si, Klothilda Lim, Yanli Liu, Yanxi Zhang, Ji Yeon Baek, Yang Liu, Deepa T. Patil, Jonathan P. Katz, Josephine Hai, Chunyang Bao, Matthew Stachler, Jun Qi, Jeffrey J. Ishizuka, Hiroshi Nakagawa, Anil K. Rustgi, Kwok-Kin Wong, Matthew Meyerson, David A. Barbie, Myles Brown, Henry Long, Adam J. Bass
Summary: The transition from normal esophageal tissue to squamous carcinoma is characterized by an altered SOX2 cistrome, leading to transcriptional reprogramming that activates endogenous retroviruses and double-stranded RNA expression, creating a dependency on the RNA editing enzyme ADAR1.
Article
Medicine, Research & Experimental
Panieh Terraf, Lynette M. Sholl, Matthew S. Davids, Mark M. Awad, Elizabeth P. Garcia, Laura E. MacConaill, Paola Dal Cin, Annette Kim, Neal Lindeman, Matthew Stachler, David H. Hwang, Adrian M. Dubuc
Summary: Comprehensive characterization of somatic genomic alterations has led to fundamental shifts in understanding of tumor biology, with clinical studies modifying diagnosis and treatment to fulfill the promise of personalized medicine. This case study of a 78-year-old woman with CLL revealed concurrent lung adenocarcinoma identified through molecular and imaging studies.
COLD SPRING HARBOR MOLECULAR CASE STUDIES
(2021)
Article
Gastroenterology & Hepatology
Mark Redston, Amy Noffsinger, Anthony Kim, Fahire G. Akarca, Marianne Rara, Diane Stapleton, Laurel Nowden, Richard Lash, Adam J. Bass, Matthew D. Stachler
Summary: This study developed a reliable criteria for grading abnormal p53 immunohistochemical expression and tested its utility as a biomarker for progression in Barrett's esophagus (BE). The findings showed that abnormal p53 expression was strongly associated with neoplastic progression in BE, regardless of histologic diagnosis and could predict progression among nondysplastic BE, indefinite for dysplasia, and low-grade dysplasia.
Review
Gastroenterology & Hepatology
Nicola F. Frei, Matthew D. Stachler
Summary: Barrett's esophagus (BE) is the precursor to esophageal adenocarcinoma (EAC), and dysplasia diagnosis is crucial for predicting progression. Recent research suggests that progression from BE to EAC is heterogeneous and can involve genome doubling and catastrophes. To overcome the lack of accurate biomarkers, focusing on genomics, epigenomics, and developing new sampling methods may be necessary for risk stratification.
Article
Genetics & Heredity
Jarrod Moore, Ryan Hekman, Benjamin C. Blum, Matthew Lawton, Sylvain Lehoux, Matthew Stachler, Douglas Pleskow, Mandeep S. Sawhney, Richard D. Cummings, Andrew Emili, Alia Qureshi
Summary: This study used precision mass spectrometry to investigate molecular perturbations in Barrett's esophagus and identified a proteomic signature that accurately classified disease status. Phosphoproteomic analysis revealed dysregulation of specific cellular processes in Barrett's esophagus.
Article
Cell Biology
Robert Odze, Nicola Frei, Amir M. Khoshiwal, Lucas C. Duits, Jacques Bergman, Matthew D. Stachler
Summary: This study aimed to evaluate whether the degree of crypt atypia in non-dysplastic Barrett's oesophagus patients correlates with progression to high-grade dysplasia/adenocarcinoma. The study showed that non-dysplastic crypts in Barrett's oesophagus are biologically abnormal, suggesting that neoplastic progression begins prior to the onset of dysplasia. The degree of crypt atypia in non-dysplastic Barrett's oesophagus patients correlates with disease progression.
Editorial Material
Oncology
Matthew D. Stachler
Summary: The study characterizes and analyzes single-cell RNA-sequencing data of the tubal gastrointestinal system, including various inflammatory conditions and intestinal metaplasia of the stomach and esophagus. The findings reveal similarities in transcript and protein levels between gastric and esophageal intestinal metaplasia. Interestingly, the study shows that individual cells within metaplastic areas can coexpress transcriptional programs of both gastric and intestinal epithelia.
Review
Biotechnology & Applied Microbiology
Annesha Chatterjee, Jordana Maria Azevedo-Mar Tins, Matthew D. Stachler
Summary: Gastric cancer is a significant global health problem, being the fifth most prevalent cancer worldwide and the fourth leading cause of cancer-related mortality. Recent progress in understanding the molecular signalling within gastric cancer, particularly in relation to IL-33, has provided potential therapeutic targets for improving survival and reducing the toxic effects of traditional therapies. IL-33 has been found to have multiple functions in gastric cancer development and progression, including promoting tumor-associated immune cells, metaplasia progression, and inducing stem cell-like and EMT properties. Targeting IL-33 could be a promising strategy for gastric cancer prevention and treatment.
ONCOTARGETS AND THERAPY
(2023)
Meeting Abstract
Medicine, General & Internal
Nicola F. Frei, Amir M. Khoshiwal, Koos H. Zwinderman, Adam J. Bass, Roos E. Pouw, Jacques J. Bergman, Matthew D. Stachler
SWISS MEDICAL WEEKLY
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Robert Odze, Nicola Frei, Amir M. Khoshiwal, Jacques Bergman, Matthew Stachler
Meeting Abstract
Gastroenterology & Hepatology
Nicola F. Frei, Amir M. Khoshiwal, Fahire Goknur Akarca, Marianne Rara, Lucas C. Duits, Michel Hof, Aeilko Zwinderman, Cheng-Zhong Zhang, Roos E. Pouw, Adam Bass, Jacques Bergman, Matthew Stachler
Proceedings Paper
Engineering, Biomedical
Justin Law, Thomas G. Paulson, Carissa A. Sanchez, Patricia C. Galipeau, Marnix Jansen, Matthew D. Stachler, Carlo C. Maley, Yinyin Yuan
Summary: Cell detection in tumor microenvironments is crucial, but automatic detection in histopathology is challenging due to diversity in cell characteristics and staining. Deep learning is effective but often requires laborious annotations. This study shows that training on public datasets can produce competitive networks for pre-cancer tissue detection without manual annotations, accelerating early cancer research.
2021 IEEE 18TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI)
(2021)
Meeting Abstract
Gastroenterology & Hepatology
Mark Redston, Amy Noffsinger, Diane Stapleton, Laurel Stapleton, Richard Lash, Adam Bass, Matthew Stachler
