Article
Agronomy
Top Bahadur Pun, Arjun Neupane, Richard Koech
Summary: This study developed a semi-automated method to quantify root-knot nematodes (RKN) based on size using image analysis, with three novel approaches showing good performance in detecting and quantifying RKN length, with the SG method being the most suitable for this purpose.
Article
Agronomy
Henok Yimer, Julie Burbridge, Janete A. Brito, Steven A. Nadler, John J. Chitambar, Sergei A. Subbotin
Summary: A rapid method using multiplex Real-time PCR was developed to detect the peach root-knot nematode and other related species, and the presence of Seville root-knot nematode in Florida and Mexico was discovered.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Microbiology
Agata Anna Cisek, Iwona Bak, Bozena Cukrowska
Summary: Methanogenic archaea are important but often ignored in studies of human and animal intestinal microbiota. This research focused on improving the quantification of methanogens using qPCR, by refining the protocol and increasing the specificity and sensitivity of the assay. The results showed increased detection range and successful validation parameters, allowing for improved detection and quantification of methanogens in stool samples, such as chicken droppings in this study.
Article
Agronomy
Yuji Oka
Summary: In this study, it was found that the combination of fluensulfone with certain aromatic compounds can attract Meloidogyne juveniles, while fluopyram alone is attractive to Meloidogyne juveniles, particularly M. javanica.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Jin-zhuo Jian, Wen-kun Huang, Long-an Kong, Heng Jian, Sulaiman Abdulsalam, De-liang Peng, Huan Peng
Summary: A TaqMan-minor groove binder (TaqMan-MGB) probe-based fluorescence quantitative real-time PCR (qPCR) method was successfully developed for the efficient detection and quantification of H. filipjevi directly from field soils. The method showed high specificity and sensitivity, capable of detecting very low levels of H. filipjevi DNAs.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Agronomy
Chunshan Wang, Shedong Sun, Chunjiang Zhao, Zhenchuan Mao, Huarui Wu, Guifa Teng
Summary: This study proposes a cucumber root-knot nematode detection model based on the modified YOLOv5s model (YOLOv5-CMS), which enhances the model's ability to extract key features, optimizes the cluster algorithm, and improves the detection precision. Experimental results show that the YOLOv5s-CMS model achieves better performance compared to the original model, providing more intuitive and accurate data sources for breeding cucumber varieties resistant to root-knot nematode.
Article
Multidisciplinary Sciences
Theodore W. Kahn, Nicholas B. Duck, Michael T. McCarville, Laura Cooper Schouten, Kathryn Schweri, Jelena Zaitseva, Julia Daum
Summary: The study reveals that soybean lines expressing the Bacillus thuringiensis protein Cy14Ab offer significant protection against soybean cyst nematode (SCN) in both greenhouse and field trials, showing promising potential for controlling plant-parasitic nematodes (PPNs). The mechanism of action involves damaging the nematodes' intestines, similar to how Cry proteins control insects, resulting in a reduction in cyst numbers and egg counts in transgenic soybean plants expressing Cry14Ab.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Intiaz Amin Chowdhury, Guiping Yan
Summary: A rapid and accurate PCR method was developed for detecting and identifying a new species of root-lesion nematode. Species-specific primers showed high specificity in both conventional and quantitative real-time PCR assays, with high detection sensitivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Hui Feng, Can-rong Zhou, Feng Zhu, Xiu-hu Le, De-dao Jing, Paul Daly, Dong-mei Zhou, Li-hui Wei
Summary: This study provides a detailed characterization of a novel source of resistance to Meloidogyne graminicola in rice. The rice variety Huidao 5 attracts the root nematodes but hinders their movement, resulting in reduced nematode numbers. The resistance is also observed under upland and flooding conditions. Cross breeding experiments suggest that a dominant gene controls the resistance. This study highlights the potential of this resistance source for crop breeding.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Plant Sciences
Shixiao Xu, Pei Tian, Zhimin Jiang, Xiaoxiang Chen, Bo Li, Jutao Sun, Zhiqiang Zhang
Summary: In this study, the transcriptional dynamics and metabolic processes involved in the interaction between RKN-resistant and RKN-susceptible tobacco varieties infected with PVY MSNR were investigated using RNA-seq analysis and functional enrichment analysis. The results revealed significant differences in gene expression and metabolic pathways, providing insights into the resistance mechanisms of tobacco against PVY MSNR.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Miguel Talavera, Tim C. Thoden, Maria D. Vela-Delgado, Soledad Verdejo-Lucas, Sara Sanchez-Moreno
Summary: The novel sulfonamide nematicide fluazaindolizine showed high selectivity towards root-knot nematodes, with minimal impact on other plant-parasitic nematodes. However, there were some slight adverse effects on soil functions as indicated by changes in certain soil food web indices.
PEST MANAGEMENT SCIENCE
(2021)
Article
Plant Sciences
Bisho Ram Lawaju, Guiping Yan, Jonathan Whitworth
Summary: This study developed a droplet digital PCR (ddPCR) assay to detect and quantify the stubby root nematode (Paratrichodorus allius) in soil. The assay showed high sensitivity and specificity, accurately detecting and quantifying nematode populations. The results suggest that ddPCR has potential as a new research tool for diagnosing plant-parasitic nematodes.
Article
Cell Biology
Rahim Hassanaly-Goulamhoussen, Ronaldo De Carvalho Augusto, Nathalie Marteu-Garello, Arthur Pere, Bruno Favery, Martine Da Rocha, Etienne G. J. Danchin, Pierre Abad, Christoph Grunau, Laetitia Perfus-Barbeoch
Summary: The root-knot nematode Meloidogyne incognita adapts rapidly to unfavorable conditions through epigenetic mechanisms, impacting gene expression and sharing similar epigenetic signatures with the model nematode Caenorhabditis elegans. The H3K4me3 histone modification is strongly correlated with high expression of genes during specific developmental stages, providing insights into the dynamic regulation of parasitism genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
M. S. Soliman, M. M. El-Deriny, D. S. S. Ibrahim, H. Zakaria, Y. Ahmed
Summary: The study demonstrates that Arthrobotrys oligospora has significant suppressive and predacious effects against root-knot nematode, M. incognita, while also promoting plant growth.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Plant Sciences
Wentao Wu, Kunhao Ye, Shaofang Zhou, Liwei Guo, Shusheng Zhu, Youyong Zhu, Yang Wang, Xiahong He
Summary: The growth of the Chinese herbal medicine industry has led to the emergence of new pests and diseases. An unidentified root-knot nematode, Meloidogyne hapla, has become a significant pest in Yunnan and Sichuan provinces, affecting monkshood production. This is the first report of M. hapla infecting monkshood in these provinces.
Article
Plant Sciences
A. K. Lees, D. M. Roberts, J. Lynott, L. Sullivan, J. L. Brierley
Article
Soil Science
Sandra Dalla Pasqua, Rosangela Dallemole-Giaretta, Idalmir dos Santos, Drieli A. Reiner, Everaldo A. Lopes
APPLIED SOIL ECOLOGY
(2020)
Article
Zoology
Gerardo Guerrero-Jimenez, Patrizia Elena Vannucchi, Marcelo Silva-Briano, Araceli Adabache-Ortiz, Roberto Rico-Martinez, David Roberts, Roy Neilson, Manuel Elias-Gutierrez
Review
Agronomy
Valeria Orlando, Ivan G. Grove, Simon G. Edwards, Thomas Prior, David Roberts, Roy Neilson, Matthew Back
Article
Zoology
David M. Roberts, Brian Boag, Fraser Hunter, Jamie Tarlton, Katrin Mackenzie, Roy Neilson
Article
Zoology
Valeria Orlando, Simon G. Edwards, Roy Neilson, Tom Prior, David Roberts, Matthew Back
Summary: Comparing six common DNA extraction protocols, it was found that the glass beads-based DNA extraction method was efficient for obtaining high quality DNA samples of Pratylenchus penetrans and other species (P. crenatus, P. neglectus, and P. thornei) at a low cost and with rapid sample throughput.
Article
Environmental Sciences
Stefanie N. Vink, Damian Bienkowski, David M. Roberts, Tim J. Daniell, Roy Neilson
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Soil Science
Roy Neilson, Sandra Caul, Fiona C. Fraser, Dale King, Susan M. Mitchell, David M. Roberts, Madeline E. Giles
APPLIED SOIL ECOLOGY
(2020)
Review
Soil Science
Everaldo Antonio Lopes, Ellen Junia Canedo, Vanessa Alves Gomes, Bruno Sergio Vieira, Douglas Ferreira Parreira, Wania Sntos Neves
Summary: Anaerobic soil disinfestation (ASD) is an ecological alternative to chemical soil fumigation, which is achieved by incorporating easily decomposable organic materials, irrigation to saturation, and soil cover with impermeable plastic to combat plant pathogens. The main mechanisms involve accumulation of toxic anaerobic decomposition products, antagonism by anaerobic organisms, and lack of oxygen.
APPLIED SOIL ECOLOGY
(2022)
Article
Agronomy
Tiago Garcia da Cunha, Ronnie Von dos Santos Veloso, Matheus Miller Mesquita de Araujo, Luciano Gabriel Tavares, Luiz Fernando Borges Ribeiro, Gislaine Pacheco Tormen, Diogo Santos Campos, Marcelo Coutinho Picanco, Everaldo Antonio Lopes, Renata Ramos Pereira, Marcus Alvarenga Soares, Ricardo Siqueira da Silva
Summary: This study identified the environmental parameters that affect the population of the leafhopper Dalbulus maidis and the transmission of diseases. It was found that the off-season planting of corn increased the incidence of virus transmission. The physiological stage of corn was identified as the main factor influencing the dynamics of D. maidis.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Rafael Henrique Fernandes, Derly Jose Henriques da Silva, Fabio Teixeira Delazari, Camilo Elber Vital, Everaldo Antonio Lopes
Summary: Anatomical, physiological, and biochemical analyses were performed to predict graft compatibility between different tomato genotypes. The concentration of abscisic acid (ABA) could be used as a reference phytohormone to predict graft compatibility among tomato genotypes.
Article
Agronomy
Tiago Garcia da Cunha, Liliane Evangelista Visotto, Leticia Mendes Pinheiro, Pedro Ivo Vieira Good God, Juliana Magrinelli Oserio Rosa, Claudio Marcelo Gongalves Oliveira, Craldo Antonio Lopes
Summary: This study investigated the distribution of Meloidogyne species in carrot fields in Brazil, revealing that Meloidogyne incognita is the most common species. A combination of morphological, biochemical, and molecular techniques proved to be an effective approach for species identification.
Article
Agriculture, Multidisciplinary
Mariana Teixeira Pigozzi, Vanessa Mendes Silva, Fabricia Queiroz Mendes, Isadora Reboucas Nolasco de Oliveira, Allan Robledo Fialho e Moraes, Everaldo Antonio Lopes
Summary: Coatings composed of maize starch and PVOH can effectively reduce postharvest losses and prolong the shelf life of papaya, with 3% maize starch coating demonstrating the best performance in this study.
CIENCIA E AGROTECNOLOGIA
(2021)
Article
Plant Sciences
Jamie N. Orr, Roy Neilson, Thomas E. Freitag, David M. Roberts, Keith G. Davies, Vivian C. Blok, Peter J. A. Cock
FRONTIERS IN PLANT SCIENCE
(2020)
