Article
Medicine, General & Internal
R. Van Rijn, I. J. Schurink, Y. de Vries, A. P. van den Berg, M. Cortes Cerisuelo, S. Darwish Murad, J. I. Erdmann, N. Gilbo, R. J. de Haas, N. Heaton, B. van Hoek, V. A. L. Huurman, I. Jochmans, O. B. van Leeuwen, V. E. de Meijer, D. Monbaliu, W. G. Polak, J. J. G. Slangen, R. I. Troisi, A. Vanlander, J. de Jonge, R. J. Porte
Summary: This study demonstrated that hypothermic oxygenated machine perfusion can reduce the risk of nonanastomotic biliary strictures following liver transplantation from donors after circulatory death, with lower rates of complications compared to conventional static cold storage.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Urology & Nephrology
Richard X. Liu, Neel Koyawala, Heather R. Thiessen-Philbrook, Mona D. Doshi, Peter P. Reese, Isaac E. Hall, Sumit Mohan, Chirag R. Parikh
Summary: In this study, we found that changes in metabolite levels before and after kidney transplantation were associated with graft failure. Alpha-ketoglutarate, 3-carboxy-4-methyl-5-propyl-2-furanpropanoate, 1-carboxyethylphenylalanine, and three glycerol-phosphatidylcholines showed significant increase in post-transplant metabolites and were significantly associated with an increased risk of graft failure. Further research on these metabolites can guide organ perfusion and reduce the discard of potentially viable organs.
KIDNEY INTERNATIONAL
(2023)
Article
Surgery
Catherine Parmentier, Samrat Ray, Laura I. Mazilescu, Masataka Kawamura, Yuki Noguchi, Emmanuel Nogueira, Sujani Ganesh, Bhranavi Arulratnam, Sangeetha N. Kalimuthu, Markus Selzner, Trevor W. Reichman
Summary: Pancreas transplantation is a curative treatment for complicated diabetes, but there is a shortage of organs. Normothermic ex vivo perfusion of the pancreas may be a solution to expand the donor pool. A study perfused six human pancreases using a previously established method, and all grafts were successfully perfused with minimal damage. This technique shows potential for safe and feasible expansion of the donor pool.
TRANSPLANT INTERNATIONAL
(2023)
Article
Immunology
Wen Hou, Shuang Yang, Jiansen Lu, Yuan Shi, Jing Chen, Decheng Chen, Fei Wang, Lei Liu
Summary: In this study, a stable intestinal hypothermic machine perfusion (HMP) system was established and it was found that HMP could significantly alleviate intestinal ischemia-reperfusion injury (IRI) and improve the outcome after intestinal transplantation (IT).
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Rebecca Panconesi, Mauricio Flores Carvalho, Janina Eden, Marilena Fazi, Fariha Ansari, Leandro Mancina, Nadia Navari, Richard Xavier Sousa Da Silva, Daniele Dondossola, Lucia Bautista Borrego, Matthias Pietzke, Adriano Peris, David Meierhofer, Paolo Muiesan, Alexander Galkin, Fabio Marra, Philipp Dutkowski, Andrea Schlegel
Summary: This study investigated the effects of NRP and HOPE on DCD liver transplantation using an animal model. The results showed that HOPE can reduce mitochondrial injury and inflammation, and improve post-transplant survival.
Article
Surgery
John F. Mulvey, Sadr ul Shaheed, Philip D. Charles, Corinna Snashall, Maria Letizia Lo Faro, Christopher W. Sutton, Ina Jochmans, Jacques Pirenne, Cees van Kooten, Henri G. D. Leuvenink, Maria Kaisar, Rutger J. Ploeg
Summary: Perfusate profiling by mass spectrometry provided biologically meaningful proteomic changes that partially explained the clinical observations of the COMPARE trial.
Article
Medicine, General & Internal
Fabian Horne, Moritz Drefs, Malte Joachim Schirren, Dominik Thomas Koch, Ganildo Cepele, Severin Johannes Jacobi, Elnaz Payani, Nikolaus Boerner, Jens Werner, Markus Otto Guba, Dionysios Koliogiannis
Summary: The study demonstrates that pre-transplant graft preconditioning with HOPE can reduce the incidence of PRS and EAD in liver transplantation, as well as improve hemodynamic stability. HOPE can also mitigate ES and prevent hyperpotassemic complications.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Sodai Sakamoto, Hiroki Bochimoto, Kengo Shibata, Nur Khatijah Mohd Zin, Moto Fukai, Kosei Nakamura, Takahisa Ishikawa, Masato Fujiyoshi, Tsuyoshi Shimamura, Akinobu Taketomi
Summary: This study aimed to verify the optimal pH of hypothermic machine perfusion (HMP) for donation after circulatory death (DCD) livers. The results showed that the HMP group with pH 7.8 displayed significant graft protection, as evidenced by lower enzyme levels, bile production, diminished tissue injury, and well-preserved mitochondrial structure. Therefore, HMP with a pH of 7.8 enhances protective effects by preserving mitochondrial structure and function, reducing reperfusion injury in DCD livers.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Immunology
Natalie Vallant, Nienke Wolfhagen, Bynvant Sandhu, Karim Hamaoui, Terence Cook, Charles Pusey, Vassilios Papalois
Summary: The study compared the effects of HMP and NMP on porcine kidneys in an ex vivo perfusion model. Results showed that HMP had better urinary output, oxygen consumption, and perfusate flow rates compared to NMP, with higher rates of cell apoptosis observed in kidneys after NMP.
Article
Surgery
Michal Grat, Marcin Morawski, Andriy Zhylko, Pawel Rykowski, Maciej Krasnodebski, Anya Wyporski, Jan Borkowski, Zbigniew Lewandowski, Konrad Kobryn, Rafal Stankiewicz, Jan Stypulkowski, Waclaw Holowko, Waldemar Patkowski, Magdalena Mielczarek-Puta, Marta Struga, Benedykt Szczepankiewicz, Barbara Gornicka, Marek Krawczyk
Summary: This study suggests that routine use of end-ischemic hypothermic oxygenated machine perfusion (HOPE) in liver transplantations from donors after brain death (DBD) is not justified, as the clinical benefits are limited to high-risk donors.
Article
Biology
Francesco Vasuri, Giuliana Germinario, Carmen Ciavarella, Michele Carroli, Ilenia Motta, Sabrina Valente, Matteo Cescon, Antonia D'Errico, Gianandrea Pasquinelli, Matteo Ravaioli
Summary: Little is known about the functions and mechanisms of endothelial cells in liver grafts, especially the effects of machine perfusion techniques. In this study, different endothelial markers were analyzed in liver grafts using immunohistochemistry and gene expression analysis. The results showed severe depression of endothelial trophism in liver grafts, which was restored after reperfusion. The execution of hypothermic oxygenated perfusion seemed to improve this recovery. These findings may contribute to improving knowledge on graft tissues and customizing perfusion techniques to improve liver transplant outcomes.
Article
Biochemistry & Molecular Biology
Karol Tejchman, Adam Nowacki, Katarzyna Kotfis, Edyta Skwirczynska, Maciej Kotowski, Labib Zair, Marek Ostrowski, Jerzy Sienko
Summary: Analysis of biomarkers during reperfusion of transplanted kidneys from brain dead donors showed that kidneys preserved with hypothermic machine perfusion had lower relative increase of certain biomarkers and better kidney function compared to those preserved with static cold storage. The long-term beneficial impact of hypothermic machine perfusion on transplanted kidneys may be due to the early modified proceedings and intensity of ischemia-reperfusion injury.
Article
Surgery
Thomas Prudhomme, Delphine Kervella, Ann Etohan Ogbemudia, Vanessa Gauttier, Stephanie Le Bas-Bernardet, David Minault, Jeremy Hervouet, Diego Cantarovich, Georges Karam, Karine Renaudin, Gilles Blancho, Julien Branchereau
Summary: This experimental study compared static cold storage (SCS) to hypothermic machine perfusion (HMP) for pancreas preservation and found that HMP may reduce exocrine injury markers. In the allotransplantation experiment, there was no significant difference in recipient survival and function between HMP and SCS groups, regardless of the preservation duration.
TRANSPLANT INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Leonie van Leeuwen, Leonie H. Venema, Raphael Heilig, Henri G. D. Leuvenink, Benedikt M. Kessler
Summary: This study investigated the effects of doxycycline on protein degradation and the urinary proteome during hypothermic machine perfusion (HMP). The results showed that doxycycline altered protein degradation during HMP, indicating its protective effects. The study also revealed metabolic and complement coagulation pathway alterations during machine perfusion, which could be targeted to attenuate ischemia-reperfusion injury (IRI).
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Review
Medicine, General & Internal
Fabio Melandro, Riccardo De Carlis, Francesco Torri, Andrea Lauterio, Paolo De Simone, Luciano De Carlis, Davide Ghinolfi
Summary: This review summarizes the current literature on viability criteria during normothermic and hypothermic machine perfusion, and discusses the future steps and evolution of these technologies.
MEDICINA-LITHUANIA
(2022)
Article
Immunology
Christophe Masset, Simon Ville, Franck Halary, Benjamin Gaborit, Celine Bressolette-Bodin, Clement Deltombe, Amaury Dujardin, Lola Jacquemont, Sabine Lebot, Delphine Kervella, Lucille Figueres, Diego Cantarovich, Magali Giral, Maryvonne Hourmant, Gilles Blancho, Claire Garandeau, Aurelie Meurette, Jacques Dantal
Summary: This study reported two cases of BKV replication in kidney transplant recipients after non-severe SARS-CoV-2 infection. Even without severe symptoms, SARS-CoV-2 infection may disrupt the immune system leading to increased viral replication, highlighting the need for monitoring in kidney transplant recipients post-infection.
TRANSPLANT INFECTIOUS DISEASE
(2021)
Article
Surgery
Thomas Prudhomme, Delphine Kervella, Ann Etohan Ogbemudia, Vanessa Gauttier, Stephanie Le Bas-Bernardet, David Minault, Jeremy Hervouet, Diego Cantarovich, Georges Karam, Karine Renaudin, Gilles Blancho, Julien Branchereau
Summary: This experimental study compared static cold storage (SCS) to hypothermic machine perfusion (HMP) for pancreas preservation and found that HMP may reduce exocrine injury markers. In the allotransplantation experiment, there was no significant difference in recipient survival and function between HMP and SCS groups, regardless of the preservation duration.
TRANSPLANT INTERNATIONAL
(2021)
Letter
Urology & Nephrology
Delphine Kervella, Lola Jacquemont, Agnes Chapelet-Debout, Clement Deltombe, Simon Ville
KIDNEY INTERNATIONAL
(2021)
Article
Urology & Nephrology
Benoit Mesnard, Maxime Leroy, James Hunter, Delphine Kervella, Marc-Olivier Timsit, Lionel Badet, Pascal Glemain, Emmanuel Morelon, Fanny Buron, Moglie Le Quintrec-Donnette, Vincent Pernin, Marc Ladriere, Sophie Girerd, Christophe Legendre, Antoine Sicard, Laeticia Albano, Stephane De Vergie, Clarisse Kerleau, Thomas Prudhomme, Jerome Rigaud, Diego Cantarovich, Gilles Blancho, Georges Karam, Magali Giral, Simon Ville, Julien Branchereau
Summary: The study assessed the impact of expanded criteria donors on urinary complications in kidney transplantation, finding that ECD donors are associated with an increased risk of urological complications, suggesting closer monitoring and preventive measures for recipients of grafts from ECD donors.
Editorial Material
Urology & Nephrology
Christophe Masset, Karine Renaudin, Delphine Kervella, Agnes Chapelet, Clement Deltombe, Simon Ville
JOURNAL OF NEPHROLOGY
(2022)
Article
Urology & Nephrology
Aurelie Hummel, Julie Oniszczuk, Delphine Kervella, Marina Charbit, Dominique Guerrot, Angelo Testa, Carole Philipponnet, Cecile Chauvet, Thomas Guincestre, Karine Brochard, Ariane Benezech, Lucile Figueres, Xavier Belenfant, Andrea Guarnieri, Nathalie Demoulin, Elisa Benetti, Marius Miglinas, Kathleen Dessaix, Johann Morelle, Andrea Angeletti, Anne-Laure Sellier-Leclerc, Bruno Ranchin, Guillaume Goussard, Laurent Hudier, Justine Bacchetta, Aude Servais, Vincent Audard
Summary: This study suggests that COVID-19 vaccination may trigger relapse of INS in rare cases, but the relapse is generally easy to control.
CLINICAL KIDNEY JOURNAL
(2022)
Review
Immunology
Delphine Kervella, Stephanie Le Bas-Bernardet, Sarah Bruneau, Gilles Blancho
Summary: The long-term survival of allografts in allotransplantation is mainly limited by antibody-mediated rejection. Chronic endothelial damages caused by this rejection are often irreversible. However, in certain cases such as ABO-incompatible transplantation and xenotransplantation, the presence of antibodies targeting graft antigens is not always associated with rejection. This resistance is believed to be associated with changes in endothelial cell phenotype and modification of the immune response. Endothelial cell protection against anti-HLA antibodies has been observed in vitro and in animal models but is not always present in immunized allograft recipients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Thibault Letellier, Delphine Kervella, Abderrahmane Sadek, Christophe Masset, Claire Garandeau, Cynthia Fourgeux, Victor Gourain, Jeremie Poschmann, Gilles Blancho, Simon Ville
Summary: This study analyzed the effects of a time-limited strategy of belatacept therapy/withdrawal of calcineurin inhibitors in kidney transplant recipients. The results showed that eGFR remained stable after belatacept withdrawal, and patients who resumed belatacept had a better eGFR trend. Additionally, the withdrawal of belatacept did not lead to rebound in alloimmune response.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Urology & Nephrology
Aurelie Moreau, Delphine Kervella, Laurence Bouchet-Delbos, Cecile Braudeau, Soraya Saiagh, Pierrick Guerif, Sophie Limou, Anne Moreau, Sylvain Bercegeay, Mathias Streitz, Birgit Sawitzki, Ben James, Paul N. Harden, David Game, Qizhi Tang, James F. Markmann, Ian S. D. Roberts, Edward K. Geissler, Brigitte Dreno, Regis Josien, Maria-Cristina Cuturi, Gilles Blancho
Summary: Survival rates of kidney transplant patients are affected by chronic rejection and side effects of immunosuppressive drugs. Cell-based immunotherapy using tolerogenic dendritic cells has shown promise in reducing general immunosuppression. A phase I clinical trial using human autologous tolerogenic dendritic cells in kidney transplantation has demonstrated the safety and effectiveness of this approach in improving graft survival.
KIDNEY INTERNATIONAL
(2023)
Review
Medicine, General & Internal
Delphine Kervella, Gilles Blancho
Summary: Immunosuppressive agents have played an important role in allogenic transplantation, but there are issues such as toxicity and long-term immune response. Current research focuses on costimulation blockade for more effective long-term immunosuppression. Treatment for antibody mediated rejection currently lacks effective immunosuppressive agents, but new drugs targeting antibodies removal and inhibiting B cells are being developed.
Article
Medicine, General & Internal
Leo Drapeau, Mathilde Beaumier, Julie Esbelin, Francois Comoz, Lucile Figueres, Giorgina Barbara Piccoli, Delphine Kervella
Summary: Pregnant patients with diabetic nephropathy are at risk of rapid kidney function decline. Guidelines for managing early diabetic nephropathy in pregnancy exist, but there is a lack of data for patients with severe nephrotic syndrome and kidney function impairment, making the decision on whether and when to start dialysis uncertain.
JOURNAL OF CLINICAL MEDICINE
(2022)
Letter
Urology & Nephrology
Christophe Masset, Claire Garandeau, Aurelie Houzet, Delphine Kervella, Simon Ville, Diego Cantarovich, Alice Leclech, Claire Leman, Raphael Gaisne, Cecile Guillot-Gueguen, Oceane Salomon, Clarisse Kerleau, Magali Giral, Jacques Dantal, Gilles Blancho
KIDNEY INTERNATIONAL REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Delphine Kervella, Benoit Mesnard, Thomas Prudhomme, Sarah Bruneau, Christophe Masset, Diego Cantarovich, Gilles Blancho, Julien Branchereau
Summary: The pancreas is highly vulnerable to ischemia-reperfusion injury, which leads to early graft losses and sterile inflammation. Controlling sterile inflammation is crucial to decrease allograft loss and improve long-term survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Transplantation
Delphine Kervella, Julien Branchereau, Thomas Prudhomme, Veronique Nerriere-Daguin, Karine Renaudin, David Minault, Jeremy Hervouet, Bernard Martinet, Sarah Bruneau, Stephanie Le Bas-Bernardet, Gilles Blancho
Summary: Presensitized patients awaiting kidney transplant have lower graft survival and longer waiting times. Ex vivo treatment of transplants by masking MHC I with the antibody JM1E3 before transplantation was found to have a partial protective effect in vitro, but was not sufficient to prevent or delay AMR in highly sensitized recipients in a pig kidney transplant model.
TRANSPLANTATION DIRECT
(2023)
Article
Surgery
Christophe Masset, Clarisse Kerleau, Gilles Blancho, Maryvonne Hourmant, Alexandre Walencik, Simon Ville, Delphine Kervella, Diego Cantarovich, Aurelie Houzet, Magali Giral, Claire Garandeau, Jacques Dantal
Summary: The choice between Basiliximab (BSX) or Anti-Thymocyte Globulin (ATG) as induction therapy in non-immunized kidney transplant recipients is still uncertain. This study compared the outcomes of non-immunized patients receiving a very low dosage of ATG versus BSX as induction therapy. The results showed that induction with a very low dose of ATG in non-immunized recipients was safe and associated with a lower rate of biopsy proven acute rejection (BPAR) and post-transplant diabetes (PTD) without increasing infectious complications.
TRANSPLANT INTERNATIONAL
(2023)