Article
Biochemical Research Methods
Hua Zhang, Xudong Shi, Yuan Liu, Bin Wang, Meng Xu, Nathan Welham, Lingjun Li
Summary: Spatial visualization of N-glycans in clinical tissue samples is achieved through a novel on-tissue derivatization strategy. This strategy utilizes the amidation reaction between sialic acid residues and aniline to stabilize and enhance the signal intensity of sialylated N-glycans. Proof-of-concept experiments and mass spectrometry imaging demonstrate the improved detection sensitivity and characterization of N-glycan dysregulation in cancer tissue samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Spectroscopy
Alba Hykollari, Katharina Paschinger, Iain B. H. Wilson
Summary: In the past 20 years, the approaches for N-glycan analysis have undergone significant changes, with mass spectrometry emerging as the predominant method in modern glycomics. Improved resolution and detection using MALDI-TOF MS have greatly contributed to the discovery of a wide range of N-glycan structures, aiding in distinguishing glycan variations.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Biochemical Research Methods
Yuanyuan Liu, Yutong Han, Wenjie Zhu, Qingming Luo, Jing Yuan, Xin Liu
Summary: Glycosylation, a common post-translational modification in mammals, particularly N-glycosylation, plays a crucial role in various processes during the development of the mammalian central nervous system. N-glycosylation changes have been observed in neuropathological conditions such as epilepsy and Alzheimer's disease. In this study, N-glycan chains were extracted from different regions of the mouse brain and analyzed using high-throughput MALDI-TOF/MS combined with Fmoc-OSU derivatization to improve glycan detection sensitivity. The study identified 96 N-glycan moieties, including high mannose, fucosylated, sialylated, and galactosylated N-glycans, with significant differences between regions. The comprehensive mapping of glycans in the brain can provide insights into glycan-mediated mechanisms and potential biomarkers for brain-associated pathologies.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yueyue Li, Ye Peng, Haojie Lu
Summary: The translation explores the significance of protein glycosylation in diversifying protein function and highlights the importance of analyzing N-glycans and linked isomers of sialylated N-glycans. The review focuses on the role of α-2,3 and α-2,6-linked sialylated N-glycans in various physiological and pathological processes, as well as the application of mass spectrometry in biomedical research.
ACTA CHIMICA SINICA
(2021)
Article
Biochemical Research Methods
Martina Zahradnikova, Ivana Ihnatova, Erika Lattova, Lukas Uhrik, Eliska Stuchlikova, Rudolf Nenutil, Dalibor Valik, Monika Nalezinska, Josef Chovanec, Zbynek Zdrahal, Borivoj Vojtesek, Lenka Hernychova, Milos Novotny
Summary: Investigating N-glycans in non-mucinous ovarian cancer patients may identify potential markers associated with resistance to platinum/taxane chemotherapy. Detection sensitivity to chemotherapy is crucial for these patients.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemical Research Methods
Jennyfer Tena, Izumi Maezawa, Mariana Barboza, Maurice Wong, Chenghao Zhu, Michael Russelle Alvarez, Lee-Way Jin, Angela M. Zivkovic, Carlito B. Lebrilla
Summary: The proteins in the cell membrane of the brain are modified by glycans, and these modifications play a crucial role in cell-cell interactions. This study analyzed the N-glycome and N-glycoproteome of the brain in different regions, and discovered regional variations in glycan types. Furthermore, differences in the glycomic and glycoproteomic profiles were observed between individuals with Alzheimer's disease, hippocampal sclerosis, and no cognitive impairment.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Chemistry, Analytical
Mengxia Cheng, Hong Shu, Ye Peng, Xiaoxiao Feng, Guoquan Yan, Lei Zhang, Jun Yao, Huimin Bao, Haojie Lu
Summary: A comprehensive strategy combining linkage-specific derivatization and charge-sensitive separation method was developed for specific analysis of alpha-2,3-sialylated N-glycan linkage isomers. This new approach demonstrated successful sensitive profiling and reproducible quantitation of serum alpha-2,3-sialylated N-glycome from ovarian cancer patients, identifying significant changes in certain glycan structures.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Rabia Javeed, Dilshad Hussain, Fahmida Jabeen, Muhammad Salman Sajid, Batool Fatima, Muhammad Naeem Ashiq, Muhammad Najam-ul-Haq
Summary: Apo-H is a plasma glycoprotein with diagnostic value for disease detection. An efficient method for analyzing Apo-H N-glycans was developed, involving sample preparation, enrichment, and detection. Selective derivatization of sialic acids was used to stabilize them for analysis, with potential applications for large clinical cohorts.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Davide Ret, Linda Stefenatti, Alessio Gentile, Johanna Rohrhofer, Simone Knaus, Eva Untersmayr
Summary: This study presents a method for stabilizing sialic acid through modification, allowing for fast and cost-effective structural analysis of N-glycans in serum glycoproteins. The method was validated in murine sera and intestinal lavages.
Article
Biochemistry & Molecular Biology
Kayla Templeton, Meiby Ramos, Jacqueline Rose, Bryan Le, Qingwen Zhou, Amin Cressman, Stephanie Ferreyra, Carlito B. Lebrilla, Fernando Antonio Fierro
Summary: The study demonstrates that sialylation of N-glycans in MSCs is tightly regulated in response to environmental cues, with the inhibition of sialylation by 3F-Neu5Ac resulting in increased adhesion, migration, and survival of MSCs, while also inhibiting osteogenic and adipogenic differentiation. Additionally, the secretion of key trophic factors by MSCs is variably affected upon exposure to 3F-Neu5Ac, suggesting glycoengineering MSCs to reduce sialylated N-glycans could enhance their therapeutic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Guillaume Font, Marie-Laure Walet-Balieu, Marie Petit, Carole Burel, Maud Maho-Vaillant, Vivien Hebert, Philippe Chan, Manuel Freret, Olivier Boyer, Pascal Joly, Sebastien Calbo, Muriel Bardor, Marie-Laure Golinski
Summary: This study found that patients with pemphigus have specific changes in their IgG N-glycome, but these changes may be more related to susceptibility to autoimmunity rather than signs of inflammation.
Article
Chemistry, Analytical
Yiting Wang, Yang Hu, Ru Wang, Wei Zhang, Huiting Mao, Chuanjun Yuan, Ruinian Hua
Summary: In this study, a highly sensitive non-proteinuria assay platform was developed using urinary sialic acid as a biomarker to accurately diagnose diabetic nephropathy within a short time period. This platform incorporated the ninhydrin reaction and the inner filter effect, resulting in stable and high sensitivity with 100% positive prediction for urine samples.
Article
Chemistry, Analytical
Yiting Wang, Yang Hu, Ru Wang, Wei Zhang, Huiting Mao, Chuanjun Yuan, Ruinian Hua
Summary: Diabetic nephropathy (DN), the most common microvascular complication associated with incurable diabetes, can now be accurately diagnosed within 20 minutes using an ultrasensitive non-proteinuria assay platform. This platform utilizes urinary sialic acid as an effective biomarker for DN and combines mimetic immunoassay and inner filter effect to convert imperceptible color into highly sensitive fluorescence signals.
Article
Chemistry, Analytical
Xiaoxiao Feng, Hong Shu, Shu Zhang, Ye Peng, Lei Zhang, Xinyi Cao, Liming Wei, Haojie Lu
Summary: A novel quantitative strategy was developed in this study to unambiguously identify and relatively quantify linkage-specific N-glycopeptides using ion mobility mass spectrometry (IM-MS). The method showed good linearity and reproducibility in relative quantification of sialic acid isomers, and aberrant sialylation of haptoglobin (Hp) in hepatocellular carcinoma (HCC) was demonstrated.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Tana V. Palomino, David C. Muddiman
Summary: This study presents a new rule that can predict the number of sialic acids on a glycan. By utilizing infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in negative-ion mode mass spectrometry, the number of sialic acids on the glycan can be predicted based on the experimental isotopic distribution of a detected glycan. This new rule improves the capability of IR-MALDESI to analyze sialylated N-linked glycans within biological tissues, providing confident glycan annotations and compositions.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Jia Guo, Changmei Liu, Xiaoman Zhou, Xiaoqiang Xu, Linhong Deng, Xiang Li, Feng Guan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Review
Cell Biology
Minxing Ma, Yutong Fu, Xiaoman Zhou, Feng Guan, Yi Wang, Xiang Li
CELLULAR SIGNALLING
(2019)
Review
Cell Biology
Xiaoman Zhou, Ganglong Yang, Feng Guan
Article
Cell Biology
Xiaoman Zhou, Yanhong Zhai, Changmei Liu, Ganglong Yang, Jia Guo, Guang Li, Chengwen Sun, Xiaowei Qi, Xiang Li, Feng Guan
CELL COMMUNICATION AND SIGNALING
(2020)
Article
Chemistry, Analytical
Xinyue Zhao, Lulu Ning, Xiaoman Zhou, Zhihui Song, Jianjian Zhang, Feng Guan, Xiao-Feng Yang
Summary: Hydrogen sulfide (H2S) is a vital endogenous signal molecule with critical physiological functions, but its site-specific delivery and release in biological systems remains a challenge. The newly developed Pro-S donor shows excellent release efficiency and live-cell compatibility, allowing real-time capturing of H2S and demonstrating anti-inflammatory effects in cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Zengqi Tan, Yazhuo Jiang, Liang Liang, Jinpeng Wu, Lin Cao, Xiaoman Zhou, Zhihui Song, Zhenyu Ye, Ziyan Zhao, Hui Feng, Zewen Dong, Shuai Lin, Zhangjian Zhou, Yili Wang, Xiang Li, Feng Guan
Summary: This study reveals the pro-metastatic and proliferative function of upregulated glycosyltransferase C1GLAT1, and provides preliminary data on mechanisms underlying dysregulation of C1GALT1 via miR-1-3p / cHP1BP3 axis in BLCA.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Food Science & Technology
Shijie Fu, Yanting Dang, Huilin Xu, Aimin Li, Xiaoman Zhou, Xiaodong Gao, Zijie Li
Summary: Aloe vera-fermented beverages (AFB) have been found to prevent diet-induced obesity and associated complications. They reduce lipid droplets in cells, inhibit weight gain and fat accumulation, and lower the levels of total cholesterol, alanine aminotransferase, and interleukin 10. Additionally, AFB alters the composition of gut microbiota.
Article
Cell Biology
Yi-Shi Liu, Yicheng Wang, Xiaoman Zhou, Linpei Zhang, Ganglong Yang, Xiao-Dong Gao, Yoshiko Murakami, Morihisa Fujita, Taroh Kinoshita
Summary: Liu et al. demonstrate that precursors of specific GPI-anchored proteins, in collaboration with ER-resident ARV1, contribute to the upregulation of GPI biosynthesis. This study provides insights into the mechanism by which GPI biosynthesis is regulated.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biology
Hui-Lin Xu, Xiaoman Zhou, Shuai Chen, Si Xu, Zijie Li, Hideki Nakanishi, Xiao-Dong Gao
Summary: The rare sugar L-sorbose has been found to decrease cell viability and increase apoptotic cells in culture, and it enhances the effect of tumor chemotherapy in mouse xenograft models. It acts as a C-3 epimer of d-fructose, and it induces apoptosis by inactivating glycolytic enzyme and impairing mitochondrial function, while also downregulating the transcription of KHK-A, which attenuates the antioxidant defense mechanism in cancer cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biology
Kaijun Chi, Huilin Xu, Hanjie Li, Ganglong Yang, Xiaoman Zhou, Xiao-Dong Gao
Summary: This study compared the adaptability and yield of expressing Siglec9-Fc fusion protein in HEK293 and CHO cell lines, and optimized the culture conditions. The glycosylation, yield, dimerization, and sialic acid binding activity of the Siglec9-Fc protein produced in HEK293 and CHO were analyzed. The study found that the protein yield was slightly higher in CHO compared to HEK293, and the recombinant protein from HEK293 had more fucosylation, while CHO showed more sialylation. Both products exhibited high dimerization ratio and sialic acid binding activity. Furthermore, the study analyzed the potential ligands on cancer cell lines and bladder cancer tissue. The results are important for selecting suitable Siglec9-Fc protein expression systems and detecting related ligands.
Review
Biology
Xiaoman Zhou, Kaijun Chi, Chairui Zhang, Quan Liu, Ganglong Yang
Summary: Tumor cells accumulate sialylation in tissues, which blocks receptor and ligand binding and inhibits immune activation through binding to the Siglec receptor. Blocking the sialylation-Siglec axis in tumor tissues could alleviate the immune suppression. This review provides updated insights into the function of sialylation in tumor biology and the latest developments in sialylation-targeted tumor therapeutics.
Article
Cell Biology
Zengqi Tan, Lin Cao, Yurong Wu, Bowen Wang, Zhihui Song, Juhong Yang, Lanming Cheng, Xiaomin Yang, Xiaoman Zhou, Zhijun Dai, Xiang Li, Feng Guan
JOURNAL OF EXTRACELLULAR VESICLES
(2020)
Article
Chemistry, Multidisciplinary
Xiaoman Zhou, Jianjian Zhang, Zhihui Song, Shuxian Lu, Yuan Yu, Jing Tian, Xiang Li, Feng Guan
CHEMICAL COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Qian Wu, Yubo Cui, Guifeng Xia, Jinlong Yang, Shuming Du, Xinhong Xiong, Li Yang, Dong Xu, Xu Deng, Jiaxi Cui
Summary: Passive daytime radiative cooling (PDRC) technology, while promising for global spacing cooling, faces limitations in practical application due to reduced cooling effectiveness caused by wear and dirt contamination. Researchers have developed a renewable armor structure using fluorinated hollow glass microspheres to enhance the anti-fouling and cooling properties of PDRC coatings. The coatings exhibit high solar reflectance and thermal emittance, leading to significant temperature reduction below ambient temperature.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Qiang Bei, Bei Zhang, Kaifeng Wang, Shiming Zhang, Guichuan Xing, Clement Cabanetos
Summary: The power conversion efficiency (PCE) of organic solar cells (OSCs) has reached 20% in recent years, thanks to the advantages of organic photoactive materials. Benzothiadiazole (BT) has attracted significant research attention as a building block of OSCs. OSCs based on BT structure have shown diversity, and the increasing PCE values indicate a promising future for OSCs. This review analyzes the progress made in the last decades, focusing on effective BT small-molecules and BT polymers for OSCs. The structure-property relationships, donor-acceptor matching criteria, and morphology control approaches are discussed. The remaining challenges and a personal perspective on the future advancement of OSCs are summarized.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Rong He, Dandan Tang, Ningge Xu, Heng Liu, Kun Dou, Xuejun Zhou, Fabiao Yu
Summary: Nasopharyngeal carcinoma (NPC) is a common malignant tumor of the head and neck, with a high incidence in China and Southeast Asian countries. In this study, a novel near-infrared fluorescent probe, SNAFL-GSH, was designed and synthesized for the detection of glutathione (GSH). The probe showed selective detection of GSH and the ability to distinguish normal and cancer cells. It has great potential for application in the diagnosis and evaluation of NPC.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Zhukang Guo, Baijiang Jin, Yile Fang, Lian Jin, Song Li, Yan Deng, Zhu Chen, Hui Chen, Yuanying Zhang, Rabia Usman, Nongyue He
Summary: This study isolated primary cells that can be stably passaged from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice and used an autonomously developed automated screening instrument for efficient nucleic acid aptamer screening. The obtained aptamers were affinity verified at the cellular level. The study also investigated the impact of cell growth environment difference on the recognition ability of aptamers and confirmed the targeting ability and drug loading ability of the aptamer.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Zhongshan Yang, Qiqi Zhang, Hui Song, Xin Chen, Jiwei Cui, Yanhui Sun, Lequan Liu, Jinhua Ye
Summary: Methane chemistry is a significant area of research in catalysis. Converting methane into value-added chemicals is desirable but challenging due to its high C-H bonding energy and lack of pi bonding or unpaired electrons. Photocatalytic partial oxidation of methane to liquid oxygenates is a promising technology for achieving high efficiency and selectivity under mild conditions. The choice and activation of oxidants are crucial for its performance.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Peng Shan, Jing Liao, Jiayi Li, Chengyan Wang, Jie Zhou, Linqiang Mei, Yunlu Dai, Qiang Wang, Wenyan Yin
Summary: This study reports an oral radioprotectant Gel@GYY that combines a gelatin-based hydrogel and a pH-responsive hydrogen sulfide donor. Gel@GYY has excellent adhesion and sustained release properties, allowing it to release H2S in the gastrointestinal tract and effectively scavenge free radicals induced by radiation. It also reduces inflammation and has biodegradable properties.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Lu Liu, Ziyi Liu, Jingnan Cui, Guiling Ning, Weitao Gong
Summary: The development of new metal-free heterogeneous catalysts has been the subject of extensive research. In this study, a new Troger's base (TB)-derived porous organic polymer (POPs) was designed and prepared by integrating multifunctional monomers. The resulting polymer, which combines the advantages of pillar[5]arene macrocycle and TB moiety, exhibited enhanced catalytic performance in Knoevenagel condensation and CO2 conversion, serving as an effective metal-free catalyst. This work provides a new strategy for fabricating metal-free heterogeneous catalysts based on macrocyclic POPs.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Leyong Zhou, Changyin Yang, Weitao Dou, Tongxia Jin, Haibo Yang, Lin Xu
Summary: This review summarizes the application of micro/nanofluidic techniques in constructing supramolecular assemblies of different scales, and highlights the advantages of these techniques over conventional synthesis methods. Furthermore, it emphasizes the potential of micro/nanofluidic systems in improving our understanding of supramolecular self-assembly processes.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Yanchao Jin, Suixiaochen Chen, Peiwen Huang, Xiongjian Chen, Chun-Yan Lin, Li-Ping Li, Xiao Chen, Rui Ding, Jianxi Liu, Riyao Chen
Summary: In this study, magnesium and coconut shell carbon were used to disinfect water by adsorbing copper(II) and converting dissolved oxygen to hydrogen peroxide through Mg corrosion. The copper was enriched on the carbon surface and efficiently catalyzed the hydrogen peroxide to inactivate E.coli. The process showed promising disinfection performance both in laboratory experiments and real wastewater treatment.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Haihong Lai, Zehong Chen, Hao Zhuo, Yijie Hu, Xuan Zhao, Jiwang Yi, Hongzhi Zheng, Ge Shi, Yifan Tong, Ling Meng, Xinwen Peng, Linxin Zhong
Summary: By altering the pyrolysis route of cellulose nanofiber, the structural defects of carbon aerogels can be reduced, leading to improved compressibility and stress retention, as well as higher stress sensor sensitivity and temperature coefficient.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Lina Guo, Jinsong Ding, Wenhu Zhou
Summary: Bacteria-based tumor therapy has resurfaced due to the revolution of tumor immunotherapy, offering unique solutions for traditional tumor treatments. Bacteria with active tropism can selectively colonize tumor sites and suppress tumor growth through various mechanisms, making them attractive tools for tumor treatment. This critical review introduces recent advances in bacteria-based tumor therapy, including bacterial properties, their benefits for tumor therapy, anti-tumor mechanisms, challenges, and future perspectives.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Lan Liang, Chuanbin Wang, Xukai Lu, Yunan Sun, Beibei Yan, Ning Li, Guanyi Chen, Li'an Hou
Summary: This study investigates the interactions between phenol and benzaldehyde in the ECH process through experiments and theoretical calculations. The results show that phenol can accelerate the conversion of benzaldehyde, while benzaldehyde inhibits the conversion of phenol. The research provides a theoretical basis for the application of ECH in practical bio-oil upgrading.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Pengcheng Fan, Yuhao He, Junan Pan, Ning Sun, Qiyu Zhang, Chen Gu, Kang Chen, Weinan Yin, Longlu Wang
Summary: This study focuses on the recent progress in hydrogen evolution reaction (HER) based on photothermal effect. It introduces the main pathways of photothermal conversions applied in H2 evolution, summarizes the functions of the photothermal effect, and presents a method to improve the catalytic system through thermal means. The study also discusses the challenges and outlook in this promising area.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Leiduo Lai, Hongyu Zhou, Yichen Hong, Mengfan Luo, Yang Shi, Heng Zhang, Zhaokun Xiong, Gang Yao, Bo Lai
Summary: In this study, a facile ammonia reduction strategy was proposed and a novel FeVO3-x catalyst was synthesized for the activation of peroxymonosulfate (PMS) to degrade the pharmaceutical carbamazepine (CBZ). The results showed that the system could rapidly degrade CBZ and generate various active species involved in the degradation process.
CHINESE CHEMICAL LETTERS
(2024)
Article
Chemistry, Multidisciplinary
Tangxin Xiao, Xiuxiu Li, Liangliang Zhang, Kai Diao, Zheng-Yi Li, Xiao-Qiang Sun, Leyong Wang
Summary: This study presents the construction of a supramolecular light harvesting system based on sequential energy transfer. The system demonstrates tunable fluorescence emission through self-assembly of polymers into nanoparticles, achieving efficient two-step light harvesting.
CHINESE CHEMICAL LETTERS
(2024)
