Article
Biochemistry & Molecular Biology
Brianna D. Young, Kristen M. Varney, Paul T. Wilder, Brianna K. Costabile, Edwin Pozharski, Mary E. Cook, Raquel Godoy-Ruiz, Oliver B. Clarke, Filippo Mancia, David J. Weber
Summary: The interaction between calmodulin (CaM) and the retinol uptake receptor STRA6 involves binding of a peptide derived from BP2 to the C-lobe of CaM in low free Ca2+ concentrations. As free Ca2+ levels increase, BP2 also interacts with the N-lobe of CaM. This structural rearrangement could be relevant to vitamin A transport by the full-length (CaM)-Ca-Ca-STRA6 complex as intracellular Ca2+ concentrations approach those typical of a Ca2+-signaling event.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Franziska Greulich, Michael Wierer, Aikaterini Mechtidou, Omar Gonzalez-Garcia, N. Henriette Uhlenhaut
Summary: The SETD1A/COMPASS histone methyltransferase complex is highly enriched in activated mouse macrophages and cooperates with the glucocorticoid receptor (GR) to mediate anti-inflammatory effects. Their interaction leads to transcriptional changes in subsets of macrophage target genes.
Article
Microbiology
Yiran Ren, Chi Zhang, Ziqing Chen, Ling Lu
Summary: The life cycle of filamentous fungi involves hyphal growth and asexual reproduction, which are crucial for invasion, propagation, and virulence in pathogens, as well as for secondary metabolite production or biomass accumulation. The CCAAT-binding complex (CBC) is a transcription factor that plays a role in regulating gene expression related to sterol metabolism and conidiation in fungi. Dysfunction of CBC can lead to abnormal conidiation in submerged cultures, highlighting its importance in the transition between vegetative growth and asexual development.
Article
Cell Biology
Ayse Malci, Xiao Lin, Rodrigo Sandoval, Eckart D. Gundelfinger, Michael Naumann, Constanze Seidenbecher, Rodrigo Herrera-Molina
Summary: The study demonstrates that Np65 coordinates the functions of PMCA and iGluRs to modulate the duration and amplitude of cytosolic Ca2+ transients in dendrites and spines of hippocampal neurons.
Review
Pharmacology & Pharmacy
Jiaxing Wang, Duane D. Miller, Wei Li
Summary: This review summarizes the crystal structures of tubulin in complexes with various CBSIs, aiming to facilitate the discovery of new generations of tubulin inhibitors.
DRUG DISCOVERY TODAY
(2022)
Article
Chemistry, Inorganic & Nuclear
Loic Daronnat, Vanessa Holfeltz, Nathalie Boubals, Thomas Dumas, Philippe Guilbaud, Diego Moreno Martinez, Philippe Moisy, Sandrine Sauge-Merle, David Lemaire, Pier Lorenzo Solari, Laurence Berthon, Catherine Berthomieu
Summary: In order to enhance the understanding of the transport and accumulation mechanisms of plutonium in the body, the complexation of Pu-(IV) with the EF-hand motif of calmodulin, the most common protein calcium-binding motif in cells, was studied. The speciation of plutonium was investigated using visible and X-ray absorption spectroscopies (XAS) in solution at physiological pH (pH 7.4) and pH 6, with two variants of the calmodulin Ca binding site I, and in different media of carbonate, chloride, and nitrate solutions. Three different species of Pu were identified in the samples, including 1:1 Pu-(IV):calmodulin peptide complexes, Pu-(IV) reduction, and peptide-mediated Pu-(IV) hexanuclear clusters.
INORGANIC CHEMISTRY
(2023)
Article
Cell Biology
Zhenfen Li, Ziyue Yang, Yuanyuan Zhu, Chunmeng Fu, Ning Li, Fang Peng
Summary: This study found that Sorcin is highly expressed in HCC and negatively regulates pyroptosis, promoting the malignant behavior of HCC. The results of this study provide important scientific basis for Sorcin as a new biomarker and potential therapeutic target for HCC.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Zachary Maschmann, Siddarth Chandrasekaran, Teck Khiang Chua, Brian R. Crane
Summary: Bacterial chemoreceptors regulate the activity of the cytosolic histidine kinase CheA through mechanisms that are not well understood. This study investigates the role of peptide linkers in CheA activity and finds that substitutions in these linkers affect CheA's basal activity and activation. Extension and alteration of the linkers can rescue CheA's autophosphorylation activity. Furthermore, the linkers also influence the mobility of the substrate domains of CheA. The findings reveal the importance of linkers in CheA's structure and function.
Editorial Material
Biochemistry & Molecular Biology
Daniele Dell'Orco
Summary: The study investigated the interaction between calmodulin and peptides mimicking different structural regions of the cardiac ryanodine receptor at varying calcium concentrations. It identified the specific CaM domains that bind to the CaM-binding domain in RyR2, showing that the interaction occurs under Ca2+-saturating conditions. This methodology may have broad applicability to the study of Ca2+ signaling and diseases associated with mutations in CaM-encoding genes.
BIOCHEMICAL JOURNAL
(2021)
Article
Multidisciplinary Sciences
Guilherme G. Moreira, Francois-Xavier Cantrelle, Andrea Quezada, Filipa S. Carvalho, Joana S. Cristovao, Urmi Sengupta, Nicha Puangmalai, Ana P. Carapeto, Mario S. Rodrigues, Isabel Cardoso, Guenter Fritz, Federico Herrera, Rakez Kayed, Isabelle Landrieu, Claudio M. Gomes
Summary: The study demonstrates that S100B protein acts as an extracellular chaperone for Tau, inhibiting its aggregation and providing evidence for neuro-protective functions.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Luis Borges-Araujo, Marina E. Monteiro, Dalila Mil-Homens, Nuno Bernardes, Maria J. Sarmento, Ana Coutinho, Manuel Prieto, Fabio Fernandes
Summary: Despite its low abundance, PI(4,5)P-2 plays a key role in membrane-associated signaling events in eukaryotic cells. The presence of physiological divalent cations can affect the clustering of PI(4,5)P-2 and its interactions with proteins, which can have a significant impact on the organization and downstream interactions of PI(4,5)P-2-binding proteins in the plasma membrane. This study characterizes the effects of Ca2+ on the organization and protein-protein interactions of PI(4,5)P-2-binding proteins, providing insights into the complex regulation of PI(4,5)P-2 in cell signaling.
Review
Agronomy
Takanori Kuronuma, Hitoshi Watanabe
Summary: The occurrence of calcium (Ca2+) deficiency disorders is a severe issue in horticulture crop production. Research has shown the impact of gene expression on the occurrence of these disorders, particularly genes related to Ca2+ movement. Studying gene expression and its relation to Ca2+ deficiency disorders can help reveal causative genes in different horticulture crops.
Article
Biochemical Research Methods
Xingyue Guan, Cheng Tan, Wenfei Li, Wei Wang, D. Thirumalai
Summary: This study reveals the crucial role of water-bridged interactions in metal ion coupled protein allostery, which reduces the ruggedness of ligand exchange landscape and facilitates allosteric communication. The asymmetric response of symmetrically arranged EF-hands upon Ca2+ binding is also highlighted as essential for efficient allosteric communication.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biology
Hong Wang, Aiping Duan, Jing Zhang, Qi Wang, Yuexian Xing, Zhaohui Qin, Zhihong Liu, Jingping Yang
Summary: The study elucidates the transcription mediated by glucocorticoid receptor (GR) in a podocyte cell line model. The researchers found that direct GR bindings are enriched at super enhancers, suggesting a strategy used by GR to regulate transcription through super-enhancers and long-range interactions.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kazuo Yamasaki, Takashi Daiho, Satoshi Yasuda, Stefania Danko, Jun-ichi Kawabe, Hiroshi Suzuki
Summary: The interactions between phospholipids and Arg324 play a crucial role in modulating the function of sarcoplasmic reticulum Ca2+-ATPase. These interactions have different effects on various reaction steps, depending on their type and composition.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Krisna C. Duong-Ly, Yin-Ming Kuo, Matthew C. Johnson, Joy M. Cote, Justin M. Kollman, Jonathan Soboloff, Glenn F. Rall, Andrew J. Andrews, Jeffrey R. Peterson
JOURNAL OF CELL SCIENCE
(2018)
Review
Cell Biology
Christina K. Go, Scott Gross, Robert Hooper, Jonathan Soboloff
Article
Biochemistry & Molecular Biology
Christina K. Go, Robert Hooper, Matthew R. Aronson, Bryant Schultz, Taha Cangoz, Neeharika Nemani, Yi Zhang, Muniswamy Madesh, Jonathan Soboloff
Article
Biochemistry & Molecular Biology
Jayati Mookerjee-Basu, Robert Hooper, Scott Gross, Bryant Schultz, Christina K. Go, Elsie Samakai, Jonathan Ladner, Emmanuelle Nicolas, Yuanyuan Tian, Bo Zhou, M. Raza Zaidi, Warren Tourtellotte, Shan He, Yi Zhang, Dietmar J. Kappes, Jonathan Soboloff
Article
Cell Biology
Rabia R. Khawaja, Amit Agarwal, Masahiro Fukaya, Hey-Kyeong Jeong, Scott Gross, Estibaliz Gonzalez-Fernandez, Jonathan Soboloff, Dwight E. Bergles, Shin H. Kang
Summary: Regulation of AMPAR GluA2 content in oligodendrocyte precursor cells affects cell proliferation and regenerative behavior, serving as a potential target for myelin repair.
Article
Microbiology
Olivia R. Pilling, Joao L. Reis-Cunha, Cooper K. Grace, Alexander S. F. Berry, Matthew S. Mitchell, Jane F. Yu, Clara S. Malekshahi, Elise P. Krespan, Christina M. Go, Claudia Lombana, Yun Song, Camila C. Amorim, Alexsandro P. Lago, Lucas Carvalho, Edgar Carvalho, Dustin Brisson, Phillip Scott, Daniel Jeffares, Daniel Beiting
Summary: The authors used selective whole genome amplification (SWGA) to explore the genomic diversity of L. braziliensis directly from patient biopsies. They found high diversity among L. braziliensis genomes from Bahia, Brazil, which correlated with geographic location. By integrating these data with publicly available genome sequences from other studies, they identified variants unique to Northeast Brazil that may be linked to high regional rates of treatment failure.
Review
Biochemistry & Molecular Biology
Scott Gross, Lauren Womer, Dietmar J. Kappes, Jonathan Soboloff
Summary: This review focuses on the novel mechanisms through which STIM controls Ca2+ signals in T cell activation, and its impact on mitochondrial function and transcriptional activation for T cell differentiation and function. It also highlights areas that require further research, including the roles of PMCA and POST in controlling Orai function, and the independence of T cell development from STIM and Orai. Resolving these issues will contribute to the advancement of this field.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Meeting Abstract
Immunology
Christina K. Go, James Grayczyk, Igor E. Brodsky, Phillip A. Scott
JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
Christina K. Go, Fernanda O. Novais, Phillip Scott
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2019)
Meeting Abstract
Hematology
Ying Wang, Shan He, Yongnian Liu, Robert Hooper, Hongshuang Yu, Yuanyuan Tian, Hongxing Sun, Ran Reshef, Elizabeth O. Hexner, Jonathan Soboloff, Yi Zhang
Article
Oncology
Xuan Mo, Hanghang Zhang, Sarah Preston, Kayla Martin, Bo Zhou, Nish Vadalia, Ana M. Gamero, Jonathan Soboloff, Italo Tempera, M. Raza Zaidi
Article
Cell Biology
Dominic Tscherrig, Rajesh Bhardwaj, Daniel Biner, Jan Dernic, Daniela Ross-Kaschitza, Christine Peinelt, Matthias A. Hediger, Martin Lochner
Summary: Changes in Ca2+ levels regulate many physiological functions, and the endoplasmic reticulum is the major Ca2+ store in cells. Store-operated calcium entry (SOCE) is a refilling process that involves protein-protein interactions between Ca2+ sensing STIM in the ER and Orai proteins in the plasma membrane, forming selective Ca2+ channels. Abnormal SOCE is associated with diseases and cancers, making STIM and Orai important therapeutic targets. Novel GSK-7975A-based molecular probes have been developed for studying SOCE in different cells and settings.
Article
Cell Biology
Nicola Fameli, Cornelis van Breemen, Klaus Groschner
Summary: The study provides evidence for the importance of junctional membrane architecture in cellular function. Through modeling and prediction, it is shown that nano-scale membrane spacing plays a crucial role in junctional ER Ca2+ refilling and signaling efficiency. Additionally, interactions between Ca2+ and the phospholipid membrane surface are suggested to support interfacial Ca2+ transport and receptor targeting. This research introduces a new concept in pathophysiology.
Article
Cell Biology
Satomi Ohtsuka, Yumi Miyai, Hiroyuki Mima, Masaki Magari, Yoichi Chiba, Futoshi Suizu, Hiroyuki Sakagami, Masaki Ueno, Hiroshi Tokumitsu
Summary: This study characterized the splice variants of mouse CaMKKI3/2 and found that CaMKKI3-3 and I3-3x were functionally active and likely to be bona fide CaMKIV kinases involved in the regulation of spermiogenesis.
