Article
Cell Biology
Marie Claes, Lieve Moons
Summary: In this study, the global number and density of RGCs in different substrains of laboratory mice were determined using the RGCode tool. It was found that the number and density of RGCs vary between substrains but are not sex-dependent. Additionally, the vulnerability of RGC populations to glaucomatous injury did not differ between the two substrains in the parameters evaluated.
Article
Cell Biology
Ben Mead, Alicia Kerr, Naoki Nakaya, Stanislav I. Tomarev
Summary: This study aimed to characterize the miRNA profile of purified retinal ganglion cells (RGC) from healthy and diseased rat retina. Over 100 different miRNAs were identified in diseased retina compared to healthy retina. Manipulating candidate mRNAs after retinal injury revealed significant neuroprotection and neuritogenesis effects in RGC.
Article
Immunology
Francesca Lazzara, Rosario Amato, Chiara Bianca Maria Platania, Federica Conti, Tsung-Han Chou, Vittorio Porciatti, Filippo Drago, Claudio Bucolo
Summary: The study found that treatment with 1,25(OH)(2)D-3 can improve retinal function in age-related glaucomatous mice, reduce retinal ganglion cell death, and decrease the expression of inflammatory cytokines. This may help alleviate retinal damage in glaucoma patients and merits further clinical evaluation for the treatment of optic neuropathies.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Neurosciences
Clivia Erb, Sabrina Reinehr, Carsten Theiss, H. Burkhard Dick, Stephanie C. Joachim
Summary: Age-related diseases such as glaucoma, a leading cause of blindness, are increasing due to an aging society. A study found that heat shock protein 27 (HSP27) is associated with glaucoma, and aged mice may be more susceptible to HSP27-induced damage compared to younger mice.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Pharmacology & Pharmacy
Giovanni Luca Romano, Lucia Gozzo, Oriana Maria Maurel, Serena Di Martino, Valentina Riolo, Vincenzo Micale, Filippo Drago, Claudio Bucolo
Summary: The topical ocular administration of FLX was found to have a neuroprotective effect in a mouse model of acute retinal damage, as it counteracted the damage of retinal ganglion cells and preserved retinal function. FLX treatment also reduced the production of pro-inflammatory molecules elicited by retinal injury. Further studies are needed to support the use of FLX as a neuroprotective agent in retinal degenerative diseases.
Review
Immunology
Estefani Garcia-Rios, Marcos Nuevalos, Francisco J. Mancebo, Pilar Perez-Romero
Summary: This article provides an overview of the impact of using CMV-specific T-cell adoptive transfer on the control of CMV infection in SOT recipients, discussing different approaches to stimulate, isolate and expand CMV-specific T-cells developed over the years, and exploring the possible use of CMV adoptive cellular therapy in this SOT population.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Ophthalmology
Won-Kyu Ju, Guy A. Perkins, Keun-Young Kim, Tonking Bastola, Woo-Young Choi, Soo-Ho Choi
Summary: Glaucoma is a leading cause of irreversible blindness, involving the degeneration of retinal ganglion cells (RGCs) and their axons. Intraocular pressure (IOP) is a treatable risk factor, but lowering IOP alone is not sufficient to prevent disease progression. Understanding the structural and functional impairment of mitochondria in RGCs and their axons is an important area of research, as oxidative phosphorylation stress-mediated mitochondrial dysfunction is associated with glaucomatous neurodegeneration.
PROGRESS IN RETINAL AND EYE RESEARCH
(2023)
Article
Cell Biology
Meng Ye, Jingqiu Huang, Qianxue Mou, Jing Luo, Yuanyuan Hu, Xiaotong Lou, Ke Yao, Bowen Zhao, Qiming Duan, Xing Li, Hong Zhang, Yin Zhao
Summary: The study demonstrates that CD82 plays a neuroprotective role in glaucoma treatment, offering potential therapeutic strategies for the disease by promoting optic nerve regeneration and ameliorating axon degeneration.
CELL DEATH & DISEASE
(2021)
Article
Immunology
Anna Wiszniewsky, Laura E. Layland, Kathrin Arndts, Lisa M. Wadephul, Ruth S. E. Tamadaho, Dennis Borrero-Wolff, Valerine C. Chunda, Chi Anizette Kien, Achim Hoerauf, Samuel Wanji, Manuel Ritter
Summary: This study conducted adoptive immune cell transfer experiments into RAG2IL-2R gamma-deficient C57BL/6 mice, revealing that CD4(+) T cells have the ability to eliminate parasites and prevent inflammation at the site of infection. This provides important insights for deciphering characteristics of distinct immune cells crucial for immunity against rodent and human filarial infections.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Engineering, Biomedical
Tingting Wang, Yan Dong, Li Yao, Fan Lu, Chenxi Wen, Zhuo Wan, Li Fan, Zhelong Li, Te Bu, Mengying Wei, Xuekang Yang, Yi Zhang
Summary: This study found that diabetes aggravated atherosclerosis, and increased expression of long-chain acyl-CoA synthetase 1 (Acsl1) in macrophages played a key role. Reprogramming macrophages by knocking down Acsl1 expression had therapeutic effects on atherosclerosis in diabetic mice.
BIOACTIVE MATERIALS
(2022)
Article
Immunology
Dan Zhang, Jie Yang, Yuanhui Zhao, Jinjun Shan, Lingling Wang, Guang Yang, Susu He, Erguang Li
Summary: Respiratory syncytial virus (RSV) infection can lead to the accumulation of eosinophils and other inflammatory cells in the lungs of neonatal mice, exacerbating the pathological changes of allergic asthma. Adoptive transfer of eosinophils from asthmatic mice with RSV infection can further enhance the pulmonary inflammatory response, but this can be effectively reduced by treatment with an anti-inflammatory drug.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ewa Fuc, Dagmara Zlotkowska, Ewa Wasilewska, Barbara Wroblewska
Summary: The transfer of OVA-experienced CD4(+) T cells ameliorated the negative immune response to OVA in mice with chicken meat allergy, indicating the potential role of specific T cells in food allergies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Hina Raja, Taimur Hassan, Muhammad Usman Akram, Naoufel Werghi
Summary: This study introduces a novel strategy focusing on RGC atrophy for screening and grading the severity of glaucomatous pathologies. The proposed framework utilizes a hybrid convolutional network to extract specific regions in the retina, allowing for quantitative screening of glaucomatous subjects. The performance of this framework is rigorously tested and clinically validated, achieving high accuracy in diagnosing and grading glaucomatous progression.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Review
Neurosciences
Jennifer H. Pham, Dorota L. Stankowska
Summary: Glaucoma is a leading cause of blindness worldwide and is commonly associated with elevated intraocular pressure. Recent studies suggest that mitochondrial dysfunction plays a crucial role in glaucomatous neurodegeneration. The retina, especially the retinal ganglion cells (RGCs), relies heavily on mitochondrial energy for signal transduction, making them vulnerable to oxidative damage. Mitochondrial dysfunction and ER stress have been observed in RGCs in glaucoma models, and the potential cross-signaling and roles of MAMs need to be further investigated.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Anatomy & Morphology
Alejandro Gallego-Ortega, Maria Norte-Munoz, Johnny Di Pierdomenico, Marcelino Aviles-Trigueros, Pedro de la Villa, Francisco Javier Valiente-Soriano, Manuel Vidal-Sanz
Summary: Using specific antibodies, alpha retinal ganglion cells (RGCs) and their subtypes were identified and studied in the adult pigmented mouse retina. The alpha RGCs are distributed throughout the retina, with higher density in the temporal region. The sustained ON and OFF response subtypes are mainly located in the periphery, while the transient ON and OFF response subtypes are found in the central regions of the retina.
FRONTIERS IN NEUROANATOMY
(2022)
Article
Ophthalmology
Matthew M. Harper, Erin A. Boese, Randy H. Kardon, Johannes Ledolter, Markus H. Kuehn
Summary: There was no significant difference in the expression of BDNF and TrkB between glaucomatous and control retinas, but BDNF expression was associated with the use of prostaglandin analogs, while TrkB expression was correlated with factors such as intraocular pressure, drug usage, and treatment outcomes.
CURRENT EYE RESEARCH
(2021)
Article
Veterinary Sciences
Sinisa D. Grozdanic, Tatjana Lazic, Helga Kecova, Kabhilan Mohan, Grazyna Adamus, Markus H. Kuehn
Summary: The study found that IMR-CAR patients exhibited significant immune-mediated damage in the eyes. After immunosuppressive therapy, 44% of treated patients had their blindness reversed, with 61% of all treated patients recovering and/or maintaining vision.
VETERINARY OPHTHALMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Matthew R. Cring, Kacie J. Meyer, Charles C. Searby, Adam Hedberg-Buenz, Michael Cave, Michael G. Anderson, Kai Wang, Val C. Sheffield
Summary: Bardet-Biedl syndrome (BBS) is a rare ciliopathy with no current effective treatments. This study shows that ectopic expression of the BBS1 gene rescues male infertility in BBS patients, but does not protect against retinal degeneration. BBS is a genetically heterogeneous disease with various clinical features.
Article
Ophthalmology
Markus H. Kuehn, Janice A. Vranka, David Wadkins, Thomas Jackson, Lin Cheng, Johannes Ledolter
Summary: This study found significant differences in TM cell density around the human eye, which are not related to segmental flow, and although eyes with POAG have reduced TM cellularity, they do not exhibit higher cell variability compared to normal eyes.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Carly van der Heide, Wes Goar, Kacie J. Meyer, Wallace L. M. Alward, Erin A. Boese, Nathan C. Sears, Ben R. Roos, Young H. Kwon, Adam P. DeLuca, Owen M. Siggs, Claudia Gonzaga-Jauregui, Val C. Sheffield, Kai Wang, Edwin M. Stone, Robert F. Mullins, Michael G. Anderson, Bao Jian Fan, Robert Ritch, Jamie E. Craig, Janey L. Wiggs, Todd E. Scheetz, John H. Fingert
Summary: This study aimed to discover genes causing pigment dispersion syndrome (PDS) by analyzing exome data of patients and controls, but did not find significant enrichment of mutations in melanosome-related genes in PDS patients. Conflicting data about the pathogenicity of MRAP mutations were also found, indicating the complex genetic basis of PDS that is not easily elucidated through exome analyses.
Article
Biochemistry & Molecular Biology
Oliver W. Gramlich, Cheyanne R. Godwin, David Wadkins, Benjamin W. Elwood, Markus H. Kuehn
Summary: The study suggests that early functional and molecular differences in the retina of glaucoma patients may manifest before significant RGC loss, possibly due to reduced GABA receptor signaling and neuroinflammation. These initial changes could offer new targets for early detection and intervention in glaucoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Nickolas A. Boehme, Adam Hedberg-Buenz, Nicole Tatro, Michael Bielecki, William C. Castonguay, Todd E. Scheetz, Michael G. Anderson, Laura M. Dutca
Summary: In experiments, blast exposure was found to cause damage to retinal ganglion cells in mice, starting with the loss of RGC markers and damage to RGC axons. Over time, this damage worsened, ultimately leading to the loss of RGC somas and axons.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Carly J. van der Heide, Kacie J. Meyer, Adam Hedberg-Buenz, Danielle Pellack, Nicholas Pomernackas, Hannah E. Mercer, Michael G. Anderson
Summary: The first part of the study focused on the rapid loss of BRN3A+ nuclei in nee mice, a model of congenital glaucoma. The second part explored the correlation between RGC density and nuclear size in glaucomatous mice, revealing two events influencing nuclear dynamics. High RGC density was associated with larger nuclei, while low RGC density correlated with smaller nuclei, particularly in glaucomatous nee mutants.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Endocrinology & Metabolism
Jingwei Jiang, John J. Reho, Sajag Bhattarai, Ioana Cherascu, Adam Hedberg-Buenz, Kacie J. Meyer, Fariba Tayyari, Adam J. Rauckhorst, Deng Fu Guo, Donald A. Morgan, Eric B. Taylor, Michael G. Anderson, Arlene Drack, Kamal Rahmouni
Summary: The study demonstrates that deficiency of BBSome in endothelial cells leads to endothelial dysfunction, increased body weight, abnormal hepatic lipid metabolism, and structural and functional abnormalities in the retina.
MOLECULAR METABOLISM
(2021)
Article
Ophthalmology
Wenxiang Deng, Adam Hedberg-Buenz, Dana A. Soukup, Sima Taghizadeh, Kai Wang, Michael G. Anderson, Mona K. Garvin
Summary: This study used deep learning methods to segment and quantify axons from cross-sections of mouse optic nerve, and achieved better performance than existing baseline automated methods, similar to experts. The semi-supervised approach performed superiorly and was implemented into AxonDeep.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Huilan Zeng, Alina V. Dumitrescu, David Wadkins, Benjamin W. Elwood, Oliver W. Gramlich, Markus H. Kuehn
Summary: Neuroinflammation is a significant factor in the pathophysiology of neurodegenerative diseases, including glaucoma. Pioglitazone, an agonist of PPAR gamma, shows potential in protecting retinal ganglion cells and reversing vision loss in glaucomatous retina.
Article
Medicine, General & Internal
Eabha O'Driscoll, Emily Hughes, Mustapha Irnaten, Markus Kuehn, Deborah Wallace, Colm O'Brien
Summary: Optic nerve head cupping, a feature of glaucoma, is associated with extracellular matrix remodeling and lamina cribrosa fibrosis. Peripapillary atrophy is common in glaucoma and involves the loss of retinal pigment epithelium adjacent to the optic nerve head. Under pro-fibrotic conditions, epithelial cells can undergo epithelial-to-mesenchymal transition and contribute to extracellular matrix fibrosis. This study investigates the role of this process in glaucoma and peripapillary atrophy, demonstrating the presence of retinal pigment epithelium cells in glaucomatous optic nerve heads and the potential of these cells to differentiate into fibroblast-like cells.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Ophthalmology
Colleen M. McDowell, Krishnakumar Kizhatil, Michael H. Elliott, Darryl R. Overby, Joseph Van Batenburg-Sherwood, J. Cameron Millar, Markus H. Kuehn, Gulab Zode, Ted S. Acott, Michael G. Anderson, Sanjoy K. Bhattacharya, Jacques A. Bertrand, Terete Borras, Diane E. Bovenkamp, Lin Cheng, John Danias, Michael Lucio De Ieso, Yiqin Du, Jennifer A. Faralli, Rudolf Fuchshofer, Preethi S. Ganapathy, Haiyan Gong, Samuel Herberg, Humberto Hernandez, Peter Humphries, Simon W. M. John, Paul L. Kaufman, Kate E. Keller, Mary J. Kelley, Ruth A. Kelly, David Krizaj, Ajay Kumar, Brian C. Leonard, Raquel L. Lieberman, Paloma Liton, Yutao Liu, Katy C. Liu, Navita N. Lopez, Weiming Mao, Timur Mavlyutov, Fiona McDonnell, Gillian J. McLellan, Philip Mzyk, Andrews Nartey, Louis R. Pasquale, Gaurang C. Patel, Padmanabhan P. Pattabiraman, Donna M. Peters, Vijaykrishna Raghunathan, Ponugoti Vasantha Rao, Naga Rayana, Urmimala Raychaudhuri, Ester Reina-Torres, Ruiyi Ren, Douglas Rhee, Uttio Roy Chowdhury, John R. Samples, E. Griffen Samples, Najam Sharif, Joel S. Schuman, Val C. Sheffield, Cooper H. Stevenson, Avinash Soundararajan, Preeti Subramanian, Chenna Kesavulu Sugali, Yang Sun, Carol B. Toris, Karen Y. Torrejon, Amir Vahabikashi, Janice A. Vranka, Ting Wang, Colin E. Willoughby, Chen Xin, Hongmin Yun, Hao F. Zhang, Michael P. Fautsch, Ernst R. Tamm, Abbot F. Clark, C. Ross Ethier, W. Daniel Stamer
Summary: This article highlights the importance of using mice as a valuable model system for studying conventional outflow resistance and intraocular pressure. It also outlines the minimum acceptable standards for developing, characterizing, and utilizing mouse models of open-angle ocular hypertension. These standards are expected to enhance scientific rigor and replicability of research findings.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Kacie J. Meyer, Danielle Pellack, Adam Hedberg-Buenz, Nicholas Pomernackas, Dana Soukup, Kai Wang, John H. Fingert, Michael G. Anderson
Summary: The study used a slit lamp system to photodocument the ocular tissues of mice injected with Ad5, revealing corneal damage and prolonged mobilization of clump cells on the iris surface in virus-injected eyes. These clump cells were identified as macrophages, indicating long-lasting changes in the eye environment caused by Ad5 injection.
Article
Ophthalmology
Matthew M. Harper, Nickolas Boehme, Laura M. Dutca, Michael G. Anderson
Summary: This study examined the influence of genetic background on the retinal ganglion cell (RGC) response to blast-mediated traumatic brain injury (TBI) in mice. It found significant differences in RGC response between different genetic backgrounds, indicating a strong dependency of RGC response on genetic factors.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)