Article
Pharmacology & Pharmacy
Judith Bockstiegel, Silas L. Wurnig, Jonas Engelhardt, Jana Enns, Finn K. Hansen, Guenther Weindl
Summary: The deficiency of HDAC6 does not affect the release of IL-1β mediated by NLRP3 in human immune cells, indicating that the inhibition of HDAC6 is not necessary for the function of the NLRP3 pathway. In addition, HDAC6 inhibitors have the potential to suppress inflammatory responses and inhibit the release of IL-1β by regulating Toll-like receptor (TLR) signaling pathway.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tomomi Kurane, Tetsuro Matsunaga, Tomoaki Ida, Kazuko Sawada, Akira Nishimura, Masayuki Fukui, Masayuki Umemura, Masaaki Nakayama, Naoya Ohara, Sohkichi Matsumoto, Takaaki Akaike, Goro Matsuzaki, Giichi Takaesu
Summary: The study reveals the previously unrecognized role of GRIM-19 as an essential regulator of NLRP3 inflammasome and a molecular mechanism underlying Zmp1-mediated suppression of IL-1 beta production during mycobacterial infection.
Article
Pharmacology & Pharmacy
Jiaqi Lin, Jinxuan Ren, Bin Zhu, Yi Dai, Dave Schwinn Gao, Suyun Xia, Zhenzhen Cheng, Yangyuxin Huang, Lina Yu
Summary: This study reveals the analgesic effects of Dimethyl Itaconate (DI), a derivative of itaconate, on inflammatory pain. DI can reduce mechanical allodynia and thermal hyperalgesia, inhibit neuroinflammation in peripheral and central sites, and regulate the expression of inflammatory mediators and NLRP3 inflammasome complex.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Veterinary Sciences
Pei Gao, Shiyuan Zhang, Xinxin Zhang, Chenggang Xu, Libin Chen, Lei Fan, Jinlian Ren, Qiuyan Lin, Bin Xiang, Tao Ren
Summary: This study investigates the expression and tissue distribution of S1PR1 after NDV infection and demonstrates that S1PR1 regulates IL-1 beta expression mainly through the NLRP3/caspase-1 inflammasome. The findings reveal the molecular mechanism underlying the inflammatory response induced by NDV infection.
VETERINARY RESEARCH
(2022)
Article
Microbiology
Dengjin Chen, Tianbei Tuo, Yongning Zhang, Lei Zhou, Xinna Ge, Jun Han, Xin Guo, Hanchun Yang
Summary: PRRSV and CSFV are common infectious pathogens in pig populations, posing significant threats to the healthy development of the pig industry. Vaccine immunization is the main method to prevent and control these two diseases. Studies have shown that PRRSV co-infection affects the immune response to CSFV vaccine. In this study, it was found that PRRSV and CSFV co-infection significantly increased the expression of NLRP3 and ASC, activated Caspase-1, and promoted IL-1 beta maturation, leading to the inhibition of CSFV proliferation. It was also determined that the activation of NLRP3 inflammasome is regulated by the TLR4-MyD88-NF-kappa B pathways.
VETERINARY MICROBIOLOGY
(2023)
Article
Immunology
Zi-Teng Zhang, Xiao-Lei Gu, Xin Zhao, Xian He, Hao-Wei Shi, Kun Zhang, Yi-Ming Zhang, Yi-Nan Su, Jiang-Bo Zhu, Zhi-Wei Li, Guo-Bao Li
Summary: Prior infection simulated by LPS/LTA injection resulted in latent inflammation with high cytokine levels, but did not affect animal survival or body temperature. Heat treatment induced heat stroke and death in animals without prior infection, showing no significant systemic or neuroinflammation. NLRP3 inflammasome-induced neuroinflammation was detected in animals with prior infection, and Nlrp3 knockout mice showed enhanced heat tolerance and reduced hypothalamus IL-1 beta production. IL-1 beta neutralizing antibody injection extended endotoxemic mice survival under heat stroke.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Chemistry, Multidisciplinary
Kai Chen, Qiuping Hu, Zhongcong Xie, Guang Yang
Summary: This study reveals that surgery leads to neuronal hypoactivity and impairs learning-dependent dendritic spine formation, resulting in deficits in multiple learning tasks. These neuronal and synaptic alterations are mediated by peripheral monocytes through NLRP3 inflammasome-dependent IL-1β production. Decreasing peripheral monocytes or inactivating NLRP3 inflammasomes can reduce IL-1β levels and improve neuronal and behavioral deficits.
Article
Immunology
Chaoqing Zhong, Ruiqing Wang, Mingqiang Hua, Chen Zhang, Fengjiao Han, Miao Xu, Xinyu Yang, Guosheng Li, Xiang Hu, Tao Sun, Chunyan Ji, Daoxin Ma
Summary: NLRP3 inflammasome is over-expressed and highly activated in AML bone marrow leukemia cells, promoting leukemia cell proliferation, inhibiting apoptosis and increasing resistance to chemotherapy. IL-1 beta plays a crucial role in this process. Targeting NLRP3 inflammasome may offer a novel therapeutic option for AML.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Allergy
Arinna Bertoni, Federica Penco, Hilaria Mollica, Paola Bocca, Ignazia Prigione, Anna Corcione, Davide Cangelosi, Francesca Schena, Genny Del Zotto, Adriana Amaro, Noemi Paladino, Emanuele Pontali, Marcello Feasi, Sara Signa, Marta Bustaffa, Roberta Caorsi, Serena Palmeri, Paola Contini, Raffaele De Palma, Ulrich Pfeffer, Paolo Uva, Anna Rubartelli, Marco Gattorno, Stefano Volpi
Summary: This study reveals the crucial role of IL-1 beta in driving inflammatory phenotypes in severe COVID-19 patients, whose maturation and secretion are regulated by inflammasomes. The findings suggest that targeting IL-1 beta could be an effective strategy for treating COVID-19 and provide a mechanistic explanation for the strong inflammatory manifestations associated with the disease.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Yufei Luo, Bojun Xiong, Haiping Liu, Zehong Chen, Huihui Huang, Changxi Yu, Jian Yang
Summary: Koumine inhibits NLRP3 inflammasome activation and reduces neutrophil infiltration and IL-1β production, both in vitro and in vivo. The inhibitory effect involves blocking the ROS/NF-kappa B/NLRP3 signal axis, suggesting potential clinical applications for NLRP3 inflammasome-related diseases.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, General & Internal
Hye-Mi Jang, Ji-Yeon Park, Yeon-Ji Lee, Min-Jung Kang, Sung-Gang Jo, Yu-Jin Jeong, Nam-Pyo Cho, Sung-Dae Cho, Dong-Jae Kim, Jong-Hwan Park
Summary: The study found that TLR2 is crucial for IL-1β generation and NLRP3 activation in response to P. nigrescens, with NLRP3 inflammasome playing a key role in processing pro-IL-1β into mature IL-1β. Furthermore, the production of reactive oxygen species, P2X7R activity, and release of cathepsin B are also involved in IL-1β production in BMDCs in response to P. nigrescens.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Huijeong Ahn, Gilyoung Lee, Byung-Cheol Han, Seung-Ho Lee, Geun-Shik Lee
Summary: This study found that maltol can inhibit the activation of inflammasomes, thus reducing inflammation. By inhibiting ROS production and Casp1 activity, maltol exerts its anti-inflammatory effects.
Article
Medicine, Research & Experimental
Ching-Chun Yang, Chih-Hsing Wu, Ta-Chun Lin, Yi-Ning Cheng, Chin-Sung Chang, Kuo-Ting Lee, Pei-Jane Tsai, Yau-Sheng Tsai
Summary: The study reveals an additional anti-inflammatory role for PPAR gamma in suppressing NLRP3 inflammasome activation through interaction with NLRP3.
Article
Immunology
Cara Lang, Sohini Roy, Yu Wang, Diana Graves, Yaomin Xu, C. Henrique Serezani, Michael Korrer, Young J. Kim
Summary: The expression of inflammasome-associated genes, NLRP3 and IL-1β, is enriched in specific monocyte and macrophage subtypes in tumor-infiltrating myeloid cells in Head and Neck Squamous Cell Carcinoma (HNSCC). Deletion of NLRP3 gene promotes tumor progression, while caspase-1 signaling in myeloid cells increases myeloid survival and drives tumor growth through phagocyte-mediated inflammasome signaling.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Prapakorn Wisitpongpun, Pachuen Potup, Kanchana Usuwanthim
Summary: This study investigated the effects of oleamide on macrophage polarization and NLRP3 inflammasome activation. The results showed that oleamide promoted M1 macrophage polarization and increased IL-1 beta production. Additionally, oleamide appeared to activate the NLRP3 inflammasome as a second signal. These findings reveal a new function for oleamide and potential targets for treating NLRP3-related inflammatory disorders.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Allergy
Ke Cao, Jinghua Wu, Xuemei Li, Hua Xie, Chenling Tang, Xiaodong Zhao, Shiyu Wang, Liping Chen, Wei Zhang, Yunfei An, Xin Li, Liya Lin, Ruonan Chai, Mingyan Fang, Yuanhang Yue, Xie Wang, Yuan Ding, Lina Zhou, Qin Zhao, Huanming Yang, Jian Wang, Shaoheng He, Xiao Liu
Article
Biochemical Research Methods
Wei Zhang, Longlong Wang, Ke Liu, Xiaofeng Wei, Kai Yang, Wensi Du, Shiyu Wang, Nannan Guo, Chuanchuan Ma, Lihua Luo, Jinghua Wu, Liya Lin, Fan Yang, Fei Gao, Xie Wang, Tao Li, Ruifang Zhang, Nitin K. Saksena, Huanming Yang, Jian Wang, Lin Fang, Yong Hou, Xun Xu, Xiao Liu
Article
Immunology
Xu Jiang, Shiyu Wang, Chen Zhou, Jinghua Wu, Yuhao Jiao, Liya Lin, Xin Lu, Bo Yang, Wei Zhang, Xinyue Xiao, Yueting Li, Xunyao Wu, Xie Wang, Hua Chen, Lidan Zhao, Yunyun Fei, Huaxia Yang, Wen Zhang, Fengchun Zhang, Hui Chen, Jianmin Zhang, Bin Li, Huanming Yang, Jian Wang, Xiao Liu, Xuan Zhang
JOURNAL OF AUTOIMMUNITY
(2020)
Article
Cell Biology
Xingqi Wang, Yuzhi Gao, Yu Li, Yuqing Huang, Yawen Zhu, Wei Lv, Ruzeng Wang, Lingshan Gou, Chao Cheng, Zhaojun Feng, Jun Xie, Jun Tian, Ruiqin Yao
CELL DEATH & DISEASE
(2020)
Article
Agriculture, Multidisciplinary
Xingqi Wang, Yuzhi Gao, Lei Wang, Di Yang, Wei Bu, Lingshan Gou, Jinjin Huang, Xinyue Duan, Yue Pan, Shuyan Cao, Zixuan Gao, Chao Cheng, Zhaojun Feng, Jun Xie, Ruiqin Yao
Summary: Using bioinformatics and network pharmacology, this study identified troxerutin as a compound that effectively improves ulcerative colitis in mice by reducing oxidative stress and inflammation, protecting intestinal epithelial cells and colon tissue, and regulating signaling pathways. The antioxidant and anti-inflammatory effects of troxerutin also inhibit cell apoptosis and alleviate colonic fibrosis, showcasing its potential in disease treatment.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Shiyu Wang, Longlong Wang, Yang Liu, Yonggang Zhu, Ya Liu
Summary: The study found that Tscm T cells have increased diversity, skewed gene usage, and shorter length distribution in the CDR3 region compared to other memory T cells. The top1000 clonotypes in Tscm were more public and grouped in more clusters, suggesting a wider recognition of epitope types. Additionally, self-reactive clonotypes were found to be more abundant in Tscm from type one diabetes patients.
Article
Medicine, Research & Experimental
Xing-qi Wang, Hao Chen, Yu-zhi Gao, Yan-xiu Huang, Rui-juan Zhang, Jun Xie, Yu Li, Yu-qing Huang, Ling-shan Gou, Rui-qin Yao
Summary: Levornidazole, with immunomodulatory properties, showed great therapeutic potential in treating ulcerative colitis induced by DSS by attenuating intestinal inflammation through reducing cytokine expression and promoting anti-inflammatory activity, highlighting its crucial role in ameliorating the intestinal inflammatory process.
CURRENT MEDICAL SCIENCE
(2021)
Article
Chemistry, Analytical
Shiyu Wang, Yang Liu, Yijian Li, Menghua Lv, Kai Gao, Ying He, Wenbo Wei, Yonggang Zhu, Xuan Dong, Xun Xu, Zida Li, Longqi Liu, Ya Liu
Summary: The study presents a droplet-based microfluidic platform for efficient screening and sorting of tumor antigen-specific T cells, demonstrating high throughput capability and validation through single-cell RNA sequencing. The platform offers a precise and efficient means for T cell immunotherapy, potentially promoting T cell-based anti-tumor therapies.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Menghua Lyu, Xuyang Shi, Xiaopan Liu, Yang Liu, Xijun Zhu, Lijuan Liao, Hongyan Zhao, Na Sun, Shiyu Wang, Linzhe Chen, Linyuan Fan, Qumiao Xu, Qianqian Zhu, Kai Gao, Huaying Chen, Yonggang Zhu, Zida Li, Weijin Guo, Yue Zheng, Ying Gu, Longqi Liu, Meiniang Wang, Ya Liu
Summary: Nanobodies, derived from the serum of Camelidae, offer advantages over conventional antibodies and hold promise for immunotherapy and antibody-drug development. A high-throughput nanobody screening platform is crucial for their rapid development. While droplet-based microfluidic systems have shown improved performance compared to traditional phage display technology, directly applying this technology to nanobody screening faces challenges. This study overcame these obstacles by constructing a eukaryotic display system and designing a signal capture system, resulting in successful screening of target cells expressing anti-PD-L1 nanobodies.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Run Xie, Yang Liu, Shiyu Wang, Xuyang Shi, Zhantao Zhao, Longqi Liu, Ya Liu, Zida Li
Summary: Combinatorial perturbation sequencing (CP-seq) is a high-throughput and comprehensive technology for screening combinatorial drug treatments on single cells. It utilizes the advantages of droplet microfluidics to enable efficient and multiplexed drug perturbation and transcriptome analysis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Shengyan Pu, Wenwen Wu, Dongsheng Shi, Yanqi Dai, Jun Zhang, Mingyan Zhao, Shuangyu Liu, Guihong Zheng, Xingqi Wang, Yu Yan, Jun Xie
Summary: In this study, high-performance nanorealgar quantum dots (NR QDs) with aggregation-induced emission enhancement of crystal units (CUs) were designed by adding hydrochloric acid. The enhanced fluorescence characteristics of NR QDs are associated with an optimized crystal structure obtained by accelerating the growth process of NR CUs. To improve theranostic efficiency, NR QDs were synthesized with tumor neovascular targeting peptide arginine-glycine-aspartic acid (RGD). The functional NR@RGD QDs effectively anchor into tumor endothelial cells, inhibit angiogenesis, and cut-off the nutrient-rich blood supply to the tumor, showing potential clinical value.
JOURNAL OF NANOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Yang Liu, Shiyu Wang, Menghua Lyu, Run Xie, Weijin Guo, Ying He, Xuyang Shi, Yang Wang, Jingyu Qi, Qianqian Zhu, Hui Zhang, Tao Luo, Huaying Chen, Yonggang Zhu, Xuan Dong, Zida Li, Ying Gu, Longqi Liu, Xun Xu, Ya Liu
Summary: The rapid advancement of single-cell omics technology is crucial for addressing challenges in precision medicine, with the development of an all-in-droplet method proving to be a labor-saving approach for monitoring TCR-T cells.
BIOENGINEERING-BASEL
(2022)
Article
Endocrinology & Metabolism
Yujia Liu, Yue Cheng, Na Xiang, Zhiyuan Wang, Siyu Li, Lijing Gong, Xingqi Wang
Summary: This study investigates the effects of aerobic exercise training on BKCa channel in diabetic vascular smooth muscle and the underlying mechanism. The results show that diabetic mice have increased vascular medium thickness, impaired BKCa-mediated vasodilation, higher levels of ROS, and lower expression of BKCa alpha, beta 1, Nrf2, and HO-1. Exercise training increases the expression of these markers. Furthermore, AMPK deletion leads to lower ROS levels and expression of BKCa alpha, beta 1, Nrf2, and HO-1. Therefore, aerobic exercise can promote the expression of the BKCa channel and improve vascular dysfunction in diabetic vascular smooth muscle cells through the AMPK/Nrf2/HO-1 signaling pathway.
ACTA DIABETOLOGICA
(2023)
Article
Chemistry, Analytical
Menghua Lyu, Xuyang Shi, Xiaopan Liu, Yang Liu, Xijun Zhu, Lijuan Liao, Hongyan Zhao, Na Sun, Shiyu Wang, Linzhe Chen, Linyuan Fan, Qumiao Xu, Qianqian Zhu, Kai Gao, Huaying Chen, Yonggang Zhu, Zida Li, Weijin Guo, Yue Zheng, Ying Gu, Longqi Liu, Meiniang Wang, Ya Liu
Summary: Nanobodies, derived from Camelidae serum, have advantages over conventional antibodies and hold promise for immunotherapy and antibody-drug development. A high-throughput nanobody screening platform is crucial. This study developed a eukaryotic display system for nanobody screening and used single-cell transcriptomic profiling to uncover intercellular heterogeneity.
ANALYTICAL CHEMISTRY
(2022)
Review
Pediatrics
Yue Cheng, Zhenjia Sun, Xudong Ya, Linlin Zhou, Mingxia Wang, Xingqi Wang, Yujia Liu
Summary: The aim of this study was to investigate the effect of exercise training on arterial stiffness in obese and overweight children. The results showed that exercise training improved vascular endothelial function and vascular sclerosis in overweight and obese children, especially in those with a BMI < 30 and younger than 14 years. AE had a more favorable effect on arterial stiffness.
EUROPEAN JOURNAL OF PEDIATRICS
(2022)
Article
Pharmacology & Pharmacy
Paola Orlandi, Marta Banchi, Francesca Vaglini, Marco Carli, Stefano Aringhieri, Arianna Bandini, Carla Pardini, Cristina Viaggi, Michele Lai, Greta Ali, Alessandra Ottani, Eleonora Vandini, Patrizia Guidi, Margherita Bernardeschi, Veronica La Rocca, Giulio Francia, Gabriella Fontanini, Mauro Pistello, Giada Frenzilli, Daniela Giuliani, Marco Scarselli, Guido Bocci
Summary: This study investigates the role of MC4R in melanoma and the use of the selective antagonist ML in combination with vemurafenib. The results show that ML can inhibit melanoma cell proliferation and induce apoptosis through the inhibition of ERK1/2 phosphorylation and reduction of BCL-XL expression. The combination of vemurafenib and ML exhibits a synergistic effect in vitro and inhibits tumor growth in vivo without causing adverse effects.
BIOCHEMICAL PHARMACOLOGY
(2024)
Article
Pharmacology & Pharmacy
Conor J. Bloxham, Katina D. Hulme, Fabrizio Fierro, Christian Fercher, Cassandra L. Pegg, Shannon L. O'Brien, Simon R. Foster, Kirsty R. Short, Sebastian G. B. Furness, Melissa E. Reichelt, Masha Y. Niv, Walter G. Thomas
Summary: Bitter taste receptors (T2Rs) are a type of G protein-coupled receptors that allow humans to detect aversive and toxic substances. This study characterized the functional properties of previously identified T2Rs in human cardiac tissues and their naturally occurring polymorphisms. The results showed differences in signaling among different T2R variants, and revealed a potential association between the T2R50 Tyr203 variant and cardiovascular disease.
BIOCHEMICAL PHARMACOLOGY
(2024)
Article
Pharmacology & Pharmacy
Lu Chen, Huanying Shi, Wenxin Zhang, Yongjun Zhu, Haifei Chen, Zimei Wu, Huijie Qi, Jiafeng Liu, Mingkang Zhong, Xiaojin Shi, Tianxiao Wang, Qunyi Li
Summary: This study demonstrates that Carfilzomib exhibits potent anti-tumor activity against esophageal squamous cell carcinoma (ESCC) by triggering mitochondrial apoptosis and reprogramming cellular metabolism. It has been identified that activating transcription factor 3 (ATF3) plays a crucial role as a cellular target in ESCC cells treated with Carfilzomib. Overexpression of ATF3 effectively counteracts the effects of Carfilzomib on ESCC cell proliferation, apoptosis, and metabolic reprogramming. Furthermore, ATF3 mediates the anti-tumor activity of Carfilzomib, suggesting its potential as a therapeutic agent for ESCC.
BIOCHEMICAL PHARMACOLOGY
(2024)
Review
Pharmacology & Pharmacy
Xing Zhang, Xiang Li, Ran Xia, Hong-Sheng Zhang
Summary: This review summarizes recent progress on the mechanisms of ferroptosis resistance in cancer and highlights the role of redox status and metabolism. Combination therapy for ferroptosis has great potential in treating resistant malignant tumors.
BIOCHEMICAL PHARMACOLOGY
(2024)
