Journal
CANCER AND METASTASIS REVIEWS
Volume 37, Issue 4, Pages 655-663Publisher
SPRINGER
DOI: 10.1007/s10555-018-9767-4
Keywords
Drug resistance; Heterogeneity; Cancer stem cells; Proliferation; Transient quiescence; Epithelial-to-mesenchymal transition (EMT); Integrity of membranous subcellular structures; Drug screening strategy; Cell death; Cell survival; Cancer strength; Cancer vulnerability; Maspin; Scheme 2D; 3D; mammosphere; Docetaxel; MS-275; Salinomycin
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Funding
- NIH [P30CA022453]
- Ruth Sager Memorial Fund
- KCI Pilot Project Grant [25S5Z]
- KCI Prostate Cancer Research Pilot Project Grant
- KCI Tumor Biology and Microenvironment Program Pilot Project
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The predominant cause of cancer mortality is metastasis. The major impediment to cancer cure is the intrinsic or acquired resistance to currently available therapies. Cancer is heterogeneous at the genetic, epigenetic, and metabolic levels. And, while a molecular-targeted drug may be pathway-precise, it can still fail to achieve wholesome cancer-precise toxicity. In the current review, we discuss the strategic differences between targeting the strengths of cancer cells in phenotypic plasticity and heterogeneity and targeting shared vulnerabilities of cancer cells such as the compromised integrity of membranous organelles. To better recapitulate subpopulations of cancer cells in different phenotypic and functional states, we developed a schematic combination of 2-dimensional culture (2D), 3-dimmensional culture in collagen I (3D), and mammosphere culture for stem cells (mammosphere), designated as Scheme 2D/3D/mammosphere. We investigated how the tumor suppressor maspin may limit carcinoma cell plasticity and affect their context-dependent response to drugs of different mechanisms including docetaxel, histone deacetylase (HDAC) inhibitor MS-275, and ionophore antibiotic salinomycin. We showed that tumor cell phenotypic plasticity is not an exclusive attribute to cancer stem cells. Nonetheless, three subpopulations of prostate cancer cells, enriched through Scheme 2D/3D/mammosphere, show qualitatively different drug responses. Interestingly, salinomycin was the only drug that effectively killed all three cancer cell subpopulations, irrespective of their capacity of stemness. Further, Scheme 2D/3D/mammosphere may be a useful model to accelerate the screening for curative cancer drugs while avoiding costly characterization of compounds that may have only selective toxicity to some, but not all, cancer cell subpopulations.
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