Article
Agriculture, Multidisciplinary
Tatsuo Fukao, Hideyuki Suzuki
Summary: In this study, gamma-glutamylvalylglycine was enzymatically synthesized with favorable taste properties, and its production was increased by adjusting reaction conditions and adding metal cations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Crystallography
Hackwon Do, Ying Wang, Chang Woo Lee, Wanki Yoo, Sangeun Jeon, Jisub Hwang, Min Ju Lee, Kyeong Kyu Kim, Han-Woo Kim, Jun Hyuck Lee, T. Doohun Kim
Summary: In this study, a gene encoding LgEstI was cloned from a bacterial fish pathogen, Lactococcus garvieae. The enzymatic activity and crystal structure of LgEstI were analyzed. The results showed that LgEstI has high esterase activity and the crystal structure of LgEstI was successfully determined. These findings contribute to the understanding of the function of LgEstI and provide a basis for protein engineering studies.
Article
Materials Science, Multidisciplinary
Fabien Leonard, Axel Lange, Andreas Kupsch, Bernd Randolf Mueller, Giovanni Bruno
Summary: This study investigates the anisotropy of materials' microstructure and morphology in diesel particulate filter (DPF) materials, quantifies the morphological anisotropy using a simple segmentation algorithm, and correlates the anisotropy of the pore space with the microstructure and crystallographic anisotropy of the material.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Food Science & Technology
Hye-Bin Cho, Jun-Ho Ahn, Hyeon-Gyu Yang, Jaeick Lee, Wu-Jin Park, Young-Wan Kim
Summary: The research findings indicate that the hydrolysis and transpeptidation activities of the recombinant BAGGT enzyme can be controlled by changing pH and the presence of NaCl.
FOOD SCIENCE AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jack B. Greisman, Kevin M. Dalton, Doeke R. Hekstra
Summary: Crystallography utilizes crystal diffraction of X-rays, electrons, or neutrons to obtain valuable data on atomic structure, but analysis of unconventional diffraction experiments can still pose challenges.
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2021)
Article
Biochemistry & Molecular Biology
Ran Zhang, Luhua Zheng, Licheng Zhou, Longbei Xiang, Bo Jiang, Tao Zhang, Jingjing Chen
Summary: This study cloned the GGT gene from Bacillus amyloliquefaciens SK11.001 into Bacillus subtilis WB800 to obtain a highly active alkaline GGT. Further research can be done to construct more favorable L-Theanine producing engineered bacteria through targeted mutations and promoter screening. The optimal activity of GGT enzyme was observed at pH 10.5 and 35 degrees C. GGT remained stable with more than 90% initial activity below 40 degrees C for 1 hour and retained more than 80% initial activity after 12 hours at pH 4.0-11.5. The conversion rate of L-Theanine reached 95% in the optimal reaction system containing 50 mM carbonate buffer (pH 10.5), 200 mM L-Gln, 1600 mM ethylamine hydrochloride, and 2 U/mL GGT, incubated for 5 hours at 35 degrees C and 200 rpm in a shaker, higher than B. amyloliquefaciens BH072 (40%).
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Chiharu Sano, Takafumi Itoh, Putthapong Phumsombat, Junji Hayashi, Mamoru Wakayama, Takao Hibi
Summary: The role of Trp525 in PnGGT was investigated through site-directed mutagenesis and structural analysis, revealing that substitution of Trp525 by nonaromatic residues dramatically reduced hydrolysis activity while increasing transfer activity significantly without affecting acceptor substrate preference. Crystal structure analysis indicated that Trp525 is a key residue limiting the movement of water molecules within the PnGGT active site.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Inorganic & Nuclear
Lucy M. C. Luong, Xian B. Carroll, Christopher D. Lowe, Marilyn M. Olmstead, Alan L. Balch
Summary: The crystallization of two solvates of Au11(PPh3)7(SCN)3 revealed differences in the relative orientations of the thiocyanate ligands.
INORGANICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Nagendar Pendem, Yella-Reddy Nelli, Leonie Cussol, Claude Didierjean, Brice Kauffmann, Christel Dolain, Gilles Guichard
Summary: Oligomers with a helix-turn-helix super-secondary structure were prepared by covalently bridging aliphatic oligourea foldamer helices with either rigid aromatic or flexible aliphatic spacers. The relative orientation of the helices in these dimers was studied using high-resolution X-ray diffraction analysis. Racemic crystallography was employed in some cases to facilitate crystallization and structure determination. All structures were solved using direct methods. Well-defined parallel helical hairpin motifs were observed in all cases when 4,4'-methylene diphenyl diisocyanate was used as a dimerizing agent, regardless of the primary sequence and chain length.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Agriculture, Multidisciplinary
Leonie Benninghaus, Tatjana Walter, Melanie Mindt, Joe Max Risse, Volker F. Wendisch
Summary: This study demonstrates the production of L-theanine using metabolic engineering techniques with Pseudomonas putida KT2440 from various carbon sources, showcasing the efficiency of the process utilizing heterologous enzymes. Fed-batch bioreactor cultures yielded significant titers of L-theanine, proving the feasibility of the approach for commercial production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Physical
Samiya Zaout, Salah Chafaa, Abdelkader Hellal, Ouafa Boukhemis, Latifa Khattabi, Hocine Merazig, Nadjib Chafai, Chawki Bensouici, Lamia Bendjeddou
Summary: This study synthesized and characterized two new alpha-aminophosphonates molecules, MAPH and NAPH, using single-crystal X-ray analysis, UV-Vis, and FT-IR spectroscopy. These molecules displayed excellent antioxidant potential in various in vitro assays, particularly MAPH, and showed promising anti-Alzheimer effects through inhibition of AChE and BCHE. The electronic and thermodynamic properties of the molecules were evaluated theoretically using DFT calculations, with global and local reactivity descriptors calculated and discussed.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Chemistry, Multidisciplinary
Tom Pan, Shikai Jin, Mitchell D. Miller, Anastasios Kyrillidis, George N. Phillips
Summary: This paper develops an initial pathway to a deep learning neural network approach for solving the phase problem in protein crystallography. It uses a synthetic dataset of small fragments derived from a well curated subset of solved structures in the Protein Data Bank (PDB), and produces electron density estimates of simple artificial systems directly from corresponding Patterson maps using a convolutional neural network architecture as a proof of concept.
Article
Chemistry, Physical
Camilo Serrano-Sterling, Diana Becerra, Jaime Portilla, Hugo Rojas, Mario Macias, Juan-Carlos Castillo
Summary: This study reported the stereoselective synthesis of novel (E)-2-cyano-3-(het)arylacrylamides 3 through a Knoevenagel condensation reaction, with high yields and moderate anti-cancer activity against various cancer cell lines.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Biochemistry & Molecular Biology
Davide Capelli, Giulia Cazzaniga, Matteo Mori, Antonio Laghezza, Fulvio Loiodice, Martina Quaglia, Elisa Negro, Fiorella Meneghetti, Stefania Villa, Roberta Montanari
Summary: PPAR gamma is a key target for the treatment of type 2 diabetes and metabolic syndrome. To avoid adverse effects, a new opportunity is the development of molecules that inhibit PPAR gamma phosphorylation by CDK5. The study reports the identification of new PPAR gamma binders that exhibit a non-agonist profile and prevent PPAR gamma phosphorylation by stabilizing the protein and weakly inhibiting CDK5.
Article
Crystallography
Amit Kumar, Xu Zhang, Oscar Vadas, Fisentzos A. Stylianou, Nicolas Dos Santos Pacheco, Sarah L. Rouse, Marc L. Morgan, Dominique Soldati-Favre, Steve Matthews
Summary: A model for parasitic motility has been proposed, suggesting that parasite actin is connected to surface adhesins through a glideosome-associated connector protein (GAC). GAC is responsible for transmitting force to surface adhesins, rather than generating force. Proper connection between actin and adhesins is crucial for motility and invasion.
