Article
Cell Biology
Wei Xu, Chirag H. Patel, Liang Zhao, Im-Hong Sun, Min-Hee Oh, Im-Meng Sun, Rachel S. Helms, Jiayu Wen, Jonathan D. Powell
Summary: GOT1 plays a crucial role in CD8+ T cell effector differentiation and function by maintaining redox balance and promoting purine nucleotide biosynthesis, glycolytic programming, and cytotoxic function, possibly through posttranslational regulation of HIF protein. Conversely, genetic deletion of GOT1 promotes the generation of memory CD8+ T cells.
Article
Multidisciplinary Sciences
Daniel M. Kremer, Barbara S. Nelson, Lin Lin, Emily L. Yarosz, Christopher J. Halbrook, Samuel A. Kerk, Peter Sajjakulnukit, Amy Myers, Galloway Thurston, Sean W. Hou, Eileen S. Carpenter, Anthony C. Andren, Zeribe C. Nwosu, Nicholas Cusmano, Stephanie Wisner, Nneka E. Mbah, Mengrou Shan, Nupur K. Das, Brian Magnuson, Andrew C. Little, Milan R. Savani, Johanna Ramos, Tina Gao, Stephen A. Sastra, Carmine F. Palermo, Michael A. Badgley, Li Zhang, John M. Asara, Samuel K. McBrayer, Marina Pasca di Magliano, Howard C. Crawford, Yatrik M. Shah, Kenneth P. Olive, Costas A. Lyssiotis
Summary: Cancer metabolism is rewired to support cell survival, with targeting metabolic dependencies following GOT1 inhibition leading to ferroptosis, an iron-dependent form of cell death. The study identifies a biochemical connection between GOT1, iron regulation, and ferroptosis in pancreatic cancer cells.
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Yueying Yang, Mengzhu Zheng, Fei Han, Lei Shang, Mingxue Li, Xiaoxia Gu, Hua Li, Lixia Chen
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a fatal malignant tumor for which effective treatment is lacking. Researchers have found that Ziprasidone, by inhibiting GOT1, can disrupt glutamine metabolism and redox state in PDAC cells, thereby inhibiting proliferation, preventing migration, and inducing apoptosis. Experimental results demonstrate that Ziprasidone exhibits significant anti-tumor effects in vivo.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Article
Chemistry, Medicinal
Shan Yan, Changxing Qi, Wei Song, Qianqian Xu, Lianghu Gu, Weiguang Sun, Yonghui Zhang
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal digestive system cancer. This study discovered three butyrolactone derivatives from a marine-derived fungus Aspergillus terreus, with one compound AH showing strong in vitro inhibitory activity and promising in vivo antitumor effects in a xenograft model. The findings suggest that AH could be a potential lead compound for developing anti-PDAC agents.
Article
Oncology
Ping Ren, Na Wu, Shichen Fu, Wenbin Wang, Qi Li, Qingzhou Cheng
Summary: MiR-122-5p may function as a tumor suppressor by inhibiting proliferation and glutamine metabolism, and inducing apoptosis in pancreatic cancer cells through altering the expression of ASCT2.
ANTICANCER RESEARCH
(2023)
Article
Biology
Ke Zhang, Yu-Jie Li, Lin-Jia Peng, Hui-Feng Gao, Lu-Ming Liu, Hao Chen
Summary: This study explores the role of M2 macrophage-derived exosomes in pancreatic cancer (PC). It is found that exosomal miR-193b-3p derived from M2 macrophages enhances the proliferation, migration, invasion, and glutamine uptake of PC cells by targeting TRIM62, resulting in the decrease of c-Myc ubiquitination.
Article
Cell Biology
Xiaowen Chen, Yixin Chen, Min Zhang, Hui Cheng, Huirong Mai, Meng Yi, Huanli Xu, Xiuli Yuan, Sixi Liu, Feiqiu Wen
Summary: The miR-146a-5p/USP6/GLS1 signaling pathway plays an important role in the chemoresistance of leukemia by regulating GLS1 ubiquitination and increasing GLS protein. Human umbilical cord mesenchymal stem cell exosomes also promote cell apoptosis through this signaling pathway, providing new therapeutic strategies.
CELL DEATH & DISEASE
(2022)
Article
Gastroenterology & Hepatology
Tuo Hu, Surendra K. Shukla, Enza Vernucci, Chunbo He, Dezhen Wang, Ryan J. King, Kanupriya Jha, Kasturi Siddhanta, Nicholas J. Mullen, Kuldeep S. Attri, Divya Murthy, Nina Chaika, Ravi Thakur, Scott E. Mulder, Camila G. Pacheco, Xiao Fu, Robin R. High, Fang Yu, Audrey Lazenby, Clemens Steegborn, Ping Lan, Kamiya Mehla, Dante Rotili, Sarika Chaudhary, Sergio Valente, Marco Tafani, Antonello Mai, Johan Auwerx, Eric Verdin, David Tuveson, Pankaj K. Singh
Summary: SIRT5 functions as a key tumor suppressor in PDAC, inhibiting tumor cell proliferation and correlating with a favorable prognosis. Loss of SIRT5 promotes PDAC development and malignant transformation.
Article
Multidisciplinary Sciences
Bo Wang, Long Zhao, Changjiang Yang, Yilin Lin, Shan Wang, Yingjiang Ye, Jianyuan Luo, Zhanlong Shen
Summary: This study investigates the effect of IDH1 hyperacetylation on the acidic microenvironment in colorectal cancer (CRC). The results show that increased alpha-KG affects the hydroxylation of Ago2 and mediates miR-9-5p targeting NHE1 protein. Knockdown of NHE1 inhibits CRC cell proliferation and migration by restricting intracellular H+ transport. This study reveals a novel mechanism through which IDH1 deacetylation regulates the cellular acidic microenvironment and inhibits CRC metastasis.
Article
Neurosciences
Xue-biao Wei, Wen-qiang Jiang, Ju-hao Zeng, Lin-qiang Huang, Hong-guang Ding, Yuan-wen Jing, Yong-li Han, Yi-chen Li, Sheng-long Chen
Summary: This study found that sepsis can cause severe sepsis-associated encephalopathy (SAE) and exacerbate the disease by promoting ferroptosis. The blood-brain barrier is damaged, leading to the accumulation of iron ions in the brain, oxidative stress, and ultimately cell death. NEAT1 in exosomes enhances ferroptosis stress by regulating the miR-9-5p/TFRC and GOT1 axis, further exacerbating sepsis-associated encephalopathy.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Maristella Canovai, Monica Evangelista, Alberto Mercatanti, Romina D'Aurizio, Letizia Pitto, Francesca Marrocolo, Valentina Casieri, Marco Pellegrini, Vincenzo Lionetti, Sergio Bracarda, Milena Rizzo
Summary: In this study, we found that miR-96-5p, miR-183-5p, and miR-210-3p released by DCT resistant PCa clones decrease the efficacy of DCT in PCa cells. These miRNAs were found to interact with genes including FOXO1, IGFBP3, PDCD4, PPP2CB, and INSIG1, affecting the sensitivity of DCT. Additionally, circulating miR-183-5p and miR-21-5p were identified as potential biomarkers for predicting DCT resistance in PCa patients.
CELL DEATH DISCOVERY
(2023)
Article
Oncology
Xiaoxiao Zhou, Kun Liu, Jing Cui, Jiongxin Xiong, Heshui Wu, Tao Peng, Yao Guo
Summary: The study revealed that circ-MBOAT2 regulates the development of pancreatic cancer and glutamine metabolism through the miR-433-3p/GOT1 axis, suggesting a potential therapeutic target for inhibiting tumor formation.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Qiang Zhu, Hong Zhou, Liming Wu, Zhenyuan Lai, Didi Geng, Weiwei Yang, Jie Zhang, Zhiya Fan, Weijie Qin, Yong Wang, Ruhong Zhou, Wen Yi
Summary: This study reveals that O-GlcNAcylation contributes to the growth of pancreatic cancer by regulating the metabolic activity of MDH1.
NATURE CHEMICAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Chang Liu, Changfeng Li, Yuanda Liu
Summary: Pancreatic cancer is a highly malignant disease with hidden onset and early metastasis. Chemotherapy is the primary treatment, but chemoresistance has become an urgent clinical problem. Recent studies have shown that metabolic reprogramming, including glucose, lipid, and amino acid metabolism, plays a crucial role in tumorigenesis and drug tolerance. Additionally, mitochondria, as central players in nutrient and energy metabolism, are also involved in drug resistance development. This review summarizes the metabolic changes during pancreatic cancer progression, their impact on chemoresistance, and current studies on metabolic inhibitors.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Yingying Xu, Zhiguo Yu, Hao Fu, Yuedong Guo, Ping Hu, Jianlin Shi
Summary: The new strategy of dual inhibition on glucose and glutamine metabolisms by silencing mutated gene Kras and glutaminase 1 (GLS1) via nanomaterial-based siKras and siGLS1 delivery can enhance pancreatic cancer treatment efficacy.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)